PBP10 

PBP10 is a decapeptide. PBP10 selectively binds to lipoteichoic acid (LTA), lipopolysaccharide (LPS) and phosphatidylinositol-4,5-bisphosphate (PIP₂). PBP10 penetrates cell membranes and possesses bactericidal, anti-inflammatory, cell motility-inhibiting and actin assembly-regulating activities. PBP10 is applicable to relevant research on bacterial infections, microbe-induced inflammation, skin and soft tissue infections, as well as sepsis^[1][2][3].

IC50: formyl peptide receptor 2 (FPR2)^[1]

PBP10 (2 μM; 15 min) selectively binds to LTA, LPS, and PIP₂, but does not bind to PE^[1].
PBP10 (1-18 h) exhibits bactericidal activity against E. coli SG13009 with an MIC of 12.5 μg/mL, and also shows bactericidal activity against B. subtilis ATCC 6051 with an MIC of 3.125 μg/mL. Its bactericidal activity is strongly inhibited by LPS and LTA^[1].
PBP10 (0-50 μM) inhibits the directional migration of NIH3T3 fibroblasts, human A7 melanoma cells and human neutrophils in a concentration-dependent manner^[2].
PBP10 (1-50 μM) potently inhibits thrombin-stimulated human platelet aggregation and platelet actin assembly in a concentration-dependent manner^[2].
PBP10 (5-20 μM) concentration-dependently inhibits the migration speed and actin assembly of human neutrophils stimulated by fMLP^[2].
PBP10 (0-100 µg/mL; 24 h) exhibits extremely low cytotoxicity against human keratinocyte HaCaT cells^[3].
PBP10 (2-10 µg/mL; 1 h) exhibits potent dose-dependent bactericidal activity against E. coli RS218 and S. aureus A1^[3].
PBP10 (2-10 µg/mL; 24 h) dose-dependently inhibits the production of NO and ROS in human keratinocyte HaCaT cells stimulated by LPS (HY-D1056), LTA, heat-inactivated E. coli or heat-inactivated S. aureus^[3].
PBP10 (2-10 µg/mL; 24 h) reduces IL-8 release in LPS- and LTA-stimulated human keratinocyte HaCaT cells in a dose-dependent manner^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1713.06

C₈₄H₁₂₇N₂₄O₁₅⁺

794466-43-6

Solid

RhB-Gln-Arg-Leu-Phe-Gln-Val-Lys-Gly-Arg-Arg-OH

RhB-QRLFQVKGRR-OH

Room temperature in continental US; may vary elsewhere.

Sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

• [1]. Bucki R, et al. Interaction of the gelsolin-derived antibacterial PBP 10 peptide with lipid bilayers and cell membranes. Antimicrob Agents Chemother. 2006;50(9):2932-2940.

  [2]. Cunningham CC, et al. Cell permeant polyphosphoinositide-binding peptides that block cell motility and actin assembly. J Biol Chem. 2001;276(46):43390-43399.  [Content Brief]

  [3]. Piktel E, et al. Inhibition of inflammatory response in human keratinocytes by magnetic nanoparticles functionalized with PBP10 peptide derived from the PIP2-binding site of human plasma gelsolin. J Nanobiotechnology. 2019;17(1):22. Published 2019 Feb 2.  [Content Brief]