PD-1/PD-L1-IN-63
Based on 1 Customer Validation
PD-1/PD-L1-IN-63 is an orally active PD-1/PD-L1 inhibitor with an IC50 of 9.1 nM. PD-1/PD-L1-IN-63 blocks the PD-1/PD-L1 interaction, induces cancer cell death and inhibits tumor growth. PD-1/PD-L1-IN-63 can be used in the research of melanoma.
For research use only. We do not sell to patients.
- Purity: 98.03%
- CAS No.: 2691796-92-4
- Formula: C37H41Cl3N4O3
- Molecular Weight:696.11
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Storage:
4°C, stored under nitrogen, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HeLa | IC50 |
4.1 μM
Compound: P18
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Antiproliferative activity against human HeLa cells assessed as cell viability after 48 hrs by CCK-8 assay
Antiproliferative activity against human HeLa cells assessed as cell viability after 48 hrs by CCK-8 assay
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[PMID: 33264007] |
PD-1/PD-L1-IN-63 potently inhibits PD-1/PD-L1 protein interaction with an IC50 of 9.1 nM in an HTRF binding assay[1].
PD-1/PD-L1-IN-63 binds to both purified human PD-L1 (KD = 88.3 nM) and murine PD-L1 (KD = 174 nM) proteins with similar high affinities as measured by ITC[1].
PD-1/PD-L1-IN-63 (0.05-5 μM) binds dose-dependently to purified human PD-L1 (KD = 202 nM) and murine PD-L1 (KD = 471 nM) proteins as measured by SPR[1].
PD-1/PD-L1-IN-63 (37-1000 nM; 48 h) promotes HepG2 cell death in a dose-dependent manner in a HepG2/hPD-L1 and Jurkat/hPD-1 coculture cell model, increasing HepG2 mortality by 58.2% at 1000 nM[1].
PD-1/PD-L1-IN-63 (48 h) inhibits the proliferation of MCF-7, H1299, HeLa, HepG2, and Jurkat cells in vitro with IC50 values ranging from 2.3 μM to 4.1 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HepG2/hPD-L1, Jurkat/hPD-1
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Concentration:37, 111, 333, 1000 nM
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Incubation Time:48 h
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Result:Promoted HepG2 cell death in a dose-dependent manner.
At 1000 nM, elevated the mortality rate of HepG2 cells by 58.2%, which was significantly higher than the positive control BMS-202.
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Cell Line:MCF-7, H1299, HeLa, HepG2, Jurkat
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Concentration:/
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Incubation Time:48 h
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Result:Had IC50 values of MCF-7 (3.2 μM), H1299 (3.7 μM), HeLa (4.1 μM), HepG2 (2.3 μM), Jurkat (2.8 μM).
PD-1/PD-L1-IN-63 (50-100 mg/kg; i.g.; 14 days) does not induce cardiotoxicity or myelosuppression in healthy BALB/c mice when administered via oral gavage at doses of 50 or 100 mg/kg once daily for 14 days[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6J-Pdcdem1(hPDCD1)/Smoc (n=5 per group)[1]
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Dosage:50 mg/kg; 100 mg/kg
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Administration:i.g.; daily; 14 days
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Result:Reduced tumor weight by 69.7% and tumor volume by 89.6% at 50 mg/kg compared to vehicle control.
Reduced tumor weight by 78.1% and tumor volume by 94.1% at 100 mg/kg compared to vehicle control.
Did not cause significant changes in body weight, spleen weight, or thymus weight compared to vehicle control.
Significantly increased tumor mRNA expression of CXCR3, CXCL9, and CXCL10 in the 100 mg/kg group.
Significantly reduced PD-L1 mRNA expression in both treatment groups.
Significantly increased tumor-infiltrating CD3+CD4+ and CD3+CD8+ T cell percentages in a dose-dependent manner in tumor tissue (but not spleen).
Significantly reduced tumor PD-L1 protein expression in both treatment groups.
Showed no morphological aberrations in liver and kidney via H&E staining compared to vehicle control.
Showed no significant differences in serum liver and kidney function markers compared to vehicle control.
Chemical Information
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CAS No. 2691796-92-4
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Appearance Powder
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Molecular Weight 696.11
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Formula C37H41Cl3N4O3
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Color White to off-white
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SMILES
N#CC1=CC=CC(COC2=CC(OCC3=CC=CC(C4=CC=CC=C4)=C3C)=C(C=C2CN5CCCCC5C(NCCN)=O)Cl)=C1.Cl.Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, stored under nitrogen, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
Solvent & Solubility
DMSO : 104 mg/mL (149.40 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (276 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.4366 mL | 7.1828 mL | 14.3655 mL | 35.9139 mL |
| 5 mM | 0.2873 mL | 1.4366 mL | 2.8731 mL | 7.1828 mL | |
| 10 mM | 0.1437 mL | 0.7183 mL | 1.4366 mL | 3.5914 mL | |
| 15 mM | 0.0958 mL | 0.4789 mL | 0.9577 mL | 2.3943 mL | |
| 20 mM | 0.0718 mL | 0.3591 mL | 0.7183 mL | 1.7957 mL | |
| 25 mM | 0.0575 mL | 0.2873 mL | 0.5746 mL | 1.4366 mL | |
| 30 mM | 0.0479 mL | 0.2394 mL | 0.4789 mL | 1.1971 mL | |
| 40 mM | 0.0359 mL | 0.1796 mL | 0.3591 mL | 0.8978 mL | |
| 50 mM | 0.0287 mL | 0.1437 mL | 0.2873 mL | 0.7183 mL | |
| 60 mM | 0.0239 mL | 0.1197 mL | 0.2394 mL | 0.5986 mL | |
| 80 mM | 0.0180 mL | 0.0898 mL | 0.1796 mL | 0.4489 mL | |
| 100 mM | 0.0144 mL | 0.0718 mL | 0.1437 mL | 0.3591 mL |
PD-1/PD-L1-IN-63 Related Classifications
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