2. Apoptosis
  3. Apoptosis
  4. Periplocin

Periplocin is a cardiotonic steroid isolated from root-bark Periploca sepium Bunge. Periplocin promotes tumor cell apoptosis and inhibits tumor growth. Periplocin has the potential to facilitate wound healing through the activation of Src/ERK and PI3K/Akt pathways mediated by Na/K-ATPase.

For research use only. We do not sell to patients.

CAS No. : 13137-64-9

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Solution
10 mM * 1 mL in DMSO In-stock
Solid
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10 mg In-stock
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Based on 6 publication(s) in Google Scholar

Other Forms of Periplocin:

Periplocin Related Antibodies

Top Publications Citing Use of Products

    Periplocin purchased from MedChemExpress. Usage Cited in: J Cancer. 2025 Jun 23;16(9):2970-2983.  [Abstract]

    Proliferation of K562 and THP-1 cells after 48 h and 72 h treatment with different concentrations of Periplocin (50, 100 nM).

    Periplocin purchased from MedChemExpress. Usage Cited in: J Cancer. 2025 Jun 23;16(9):2970-2983.  [Abstract]

    Flow cytometry analysis of apoptosis in K562 and THP-1 cells treated with 100 nM Periplocin for 72 h.

    Periplocin purchased from MedChemExpress. Usage Cited in: J Cancer. 2025 Jun 23;16(9):2970-2983.  [Abstract]

    Quantitative real-time PCR analysis of HDAC10 mRNA levels in K562 and THP-1 cells treated with DMSO (control) or Periplocin (Peri) (100 nM).

    Periplocin purchased from MedChemExpress. Usage Cited in: J Cancer. 2025 Jun 23;16(9):2970-2983.  [Abstract]

    Western blot analysis of HDAC10 protein expression in K562 and THP-1 cells treated with DMSO or Periplocin (100 nM).

    Periplocin purchased from MedChemExpress. Usage Cited in: Biomol Biomed. 2025 Mar 7;25(4):857-868.  [Abstract]

    The proliferation of HepG2/OXA cells treated with OXA (10 µM), Periplocin (PPLN) (80 nM), or OXA+PPLN was detected using EDU staining.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Periplocin is a cardiotonic steroid isolated from root-bark Periploca sepium Bunge. Periplocin promotes tumor cell apoptosis and inhibits tumor growth. Periplocin has the potential to facilitate wound healing through the activation of Src/ERK and PI3K/Akt pathways mediated by Na/K-ATPase[1][2].

    IC50 & Target

    Apoptosis[2]
    In Vitro

    Periplocin (5-20 μM; 48 hours; L929 cells) treatment shows increased proliferation up to 131% at 20 μM[1].
    Periplocin (5-20 μM; 30-120 minutes; L929 cells) increases phosphorylation of Src, ERK, PI3K and Akt at active sites in a dosedependent and time-dependent manner. Periplocin (5-20 μM; 48 hours) significantly promotes migration of fibroblast cell[1].
    Periplocin (5-20 μM; 48 hours) increases collagen production in L929 fibroblast[1].
    Periplocin induces Na/KATPase mediates the activation of Src/ERK and PI3K/Akt pathways[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Periplocin Related Antibodies

    Cell Viability Assay[1]

    Cell Line: L929 cells
    Concentration: 5 μM, 10 μM, 20 μM
    Incubation Time: 48 hours
    Result: Showed increased proliferation up to 131% at 20 μM.

    Western Blot Analysis[1]

    Cell Line: L929 cells
    Concentration: 5 μM, 10 μM, 20 μM
    Incubation Time: 30 minutes, 60 minutes, 120 minutes
    Result: Increased phosphorylation of Src, ERK, PI3K and Akt at active sites in a dosedependent and time-dependent manner.
    In Vivo

    Periplocin (5-20 mg/kg; intraperitoneal injection; daily; for 14 days; female SCID mice) treatment represses the growth of hepatocellular carcinoma (HCC) in xenograft tumor model in mice[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Female SCID mice (6-8 weeks old) injected with Huh-7 cells[2]
    Dosage: 5 mg/kg, 20 mg/kg
    Administration: Intraperitoneal injection; daily; for 14 days
    Result: Repressed the growth of hepatocellular carcinoma (HCC) in xenograft tumor model in mice.
    Molecular Weight

    696.82

    Formula

    C36H56O13
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C1OCC([C@H]2CC[C@]3(O)[C@]([C@]4([H])CC[C@@]32C)([H])CC[C@]([C@]4(C)CC5)(O)C[C@H]5O[C@@](O[C@@H]6C)([H])C[C@H](OC)[C@@H]6O[C@]7([H])O[C@H](CO)[C@@H](O)[C@H](O)[C@H]7O)=C1
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (143.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.4351 mL 7.1755 mL 14.3509 mL
    5 mM 0.2870 mL 1.4351 mL 2.8702 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (3.59 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (3.59 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.81%

    SDS (643 KB)

    COA (254 KB) HNMR (148 KB) LCMS (92 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.4351 mL 7.1755 mL 14.3509 mL 35.8773 mL
    5 mM 0.2870 mL 1.4351 mL 2.8702 mL 7.1755 mL
    10 mM 0.1435 mL 0.7175 mL 1.4351 mL 3.5877 mL
    15 mM 0.0957 mL 0.4784 mL 0.9567 mL 2.3918 mL
    20 mM 0.0718 mL 0.3588 mL 0.7175 mL 1.7939 mL
    25 mM 0.0574 mL 0.2870 mL 0.5740 mL 1.4351 mL
    30 mM 0.0478 mL 0.2392 mL 0.4784 mL 1.1959 mL
    40 mM 0.0359 mL 0.1794 mL 0.3588 mL 0.8969 mL
    50 mM 0.0287 mL 0.1435 mL 0.2870 mL 0.7175 mL
    60 mM 0.0239 mL 0.1196 mL 0.2392 mL 0.5980 mL
    80 mM 0.0179 mL 0.0897 mL 0.1794 mL 0.4485 mL
    100 mM 0.0144 mL 0.0718 mL 0.1435 mL 0.3588 mL
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    Periplocin Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Periplocin13137-64-9ApoptosisCrdiotonicsteroidPI3KAktSrcERKwoundhealingapoptosistumorInhibitorinhibitorinhibit

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