PolQi2

Name: PolQi2
Brand: MedChemExpress
SKU: HY-150279
Rating: 5.0 (3 reviews)

Cat. No.: HY-150279 Purity: 98.91%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

PolQi2 is a PolΘ inhibitor that targets and inhibits alt-EJ (alternative end-joining) repair by inhibiting the helicase domain at the N-terminus of PolΘ. PolQi2 enhances the precision and integration efficiency of gene editing at different loci and in various cell lines. Furthermore, the combined use of PolQi2 with DNA-PK inhibitors reduces the off-target effects of Cas9. PolQi2 can be used in gene editing research.

For research use only. We do not sell to patients.

CAS No. : 2565638-16-4

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 3 publication(s) in Google Scholar

3 Publications Citing Use of MCE PolQi2

Others

Flow Cytometry

: PolQi2 purchased from MedChemExpress. Usage Cited in: Nat Biotechnol. 2024 Nov 27. [Abstract]; Quantification by long-read sequencing of the fraction of total reads with a length greater than a defined size and the quantified frequency of deletions larger than 1 kb in edited RPE-1 p53^−/− cells was performed. Editing with combined AZD7648 and PolQi2 (3 µM; 3 d) reduced AZD7648-induced kilobase-scale deletions.

: PolQi2 purchased from MedChemExpress. Usage Cited in: Nat Biotechnol. 2024 Nov 27. [Abstract]; Editing with combined AZD7648 and PolQi2 (3 μM; 3 d) maintained elevated HDR levels. Frequencies of HDR and indels detected by short-read sequencing in RPE-1 p53^−/− and RPE-1 p53^+/+ cells edited at HBB, CCR5, and GAPDH were recorded.

: PolQi2 purchased from MedChemExpress. Usage Cited in: bioRxiv. 2025 May 13:2025.05.10.653283. [Abstract]; Quantification of Ec78 retron precise editing was performed in comparison with no reverse transcriptase control (No RT), in the absence (Ec78) or the presence (Ec78 + inhib) of inhibitors, AZD7648 and PolQi2 (3 μM; 48 h), and the same experiments were conducted with the Mestre-1531 retron (RT 1531) using the same inhibitors.

: PolQi2 purchased from MedChemExpress. Usage Cited in: bioRxiv. 2025 Nov 29.; PolQi2, a potent Polθ inhibitor, suppresses the MMEJ repair pathway and enhances HDR efficiency during CRISPR/Cas9-mediated gene editing. Splenic T cells from transgenic Cas9 mice were activated with Dynabeads and transduced with the specified MOI of AAV after 48 hours in 1 μM AZD7468 and 3 μM PolQi2. Percentage of cells expressing GFP above baseline level was determined by flow cytometry 5 days after transduction.

: PolQi2 purchased from MedChemExpress. Usage Cited in: bioRxiv. 2025 Nov 29.; Knock-in efficiency was assessed by flow cytometry as the percentage of GFP+ cells among CD3- cells. Treatment with AZD7648 and PolQi2 (3 μM) resulted in the highest editing efficiency, achieving a knock-in rate of up to 80% under AAV transduction conditions.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Helicase

In Vitro

PolQi2 (3 μM, 14 days), in combination with DNA-PK inhibitors (1 μM, 14 days), efficiently and stably achieves targeted gene insertion in HeLa and Jurkat cells, without adversely affecting cell proliferation [1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

453.90

Formula

C₂₁H₁₆ClN₅O₃S

CAS No.

2565638-16-4

Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=CC=NC=C1C2=C(OC)C=CC=C2)NC3=NN=C(S3)OCC4=NC=C(C=C4)Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 70 mg/mL (154.22 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2031 mL	11.0156 mL	22.0313 mL
5 mM	0.4406 mL	2.2031 mL	4.4063 mL
10 mM	0.2203 mL	1.1016 mL	2.2031 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (5.51 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 2.5 mg/mL (5.51 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.91%

Select Batch:

Data Sheet (274 KB) SDS (254 KB)

COA (250 KB) HNMR (138 KB) RP-HPLC (169 KB) LCMS (202 KB)

Handling Instructions (2659 KB)

References

[1]. imberger S, et al. Simultaneous inhibition of DNA-PK and Polθ improves integration efficiency and precision of genome editing. Nat Commun. 2023 Aug 14;14(1):4761. [Content Brief]

[2]. Antoniou P, et al. Improved nuclease-based prime editing by DNA repair modulation and pegRNA engineering[J]. BioRxiv, 2024: 2024.02. 01.578377.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.2031 mL	11.0156 mL	22.0313 mL	55.0782 mL
	5 mM	0.4406 mL	2.2031 mL	4.4063 mL	11.0156 mL
	10 mM	0.2203 mL	1.1016 mL	2.2031 mL	5.5078 mL
	15 mM	0.1469 mL	0.7344 mL	1.4688 mL	3.6719 mL
	20 mM	0.1102 mL	0.5508 mL	1.1016 mL	2.7539 mL
	25 mM	0.0881 mL	0.4406 mL	0.8813 mL	2.2031 mL
	30 mM	0.0734 mL	0.3672 mL	0.7344 mL	1.8359 mL
	40 mM	0.0551 mL	0.2754 mL	0.5508 mL	1.3770 mL
	50 mM	0.0441 mL	0.2203 mL	0.4406 mL	1.1016 mL
	60 mM	0.0367 mL	0.1836 mL	0.3672 mL	0.9180 mL
	80 mM	0.0275 mL	0.1377 mL	0.2754 mL	0.6885 mL
	100 mM	0.0220 mL	0.1102 mL	0.2203 mL	0.5508 mL

PolQi2 Related Classifications

Others

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PolQi22565638-16-4PolQi 2PolQi-2DNA/RNA SynthesishelicasePolθgene editingInhibitorinhibitorinhibit

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PolQi2

Customer Review

PolQi2 Related Antibodies

3 Publications Citing Use of MCE PolQi2

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All DNA/RNA Synthesis Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

PolQi2 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

PolQi2 Related Classifications

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