3,6-Diaminoacridine

Name: 3,6-Diaminoacridine
Brand: MedChemExpress
SKU: HY-B1741
Rating: 5.0 (3 reviews)

Cat. No.: HY-B1741 Purity: 98.78%: Data Sheet
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3,6-Diaminoacridine is a versatile acridine compound. 3,6-Diaminoacridine exerts antibacterial activity by intercalating into bacterial DNA, interfering with replication and transcription, and inducing bacterial lysis. 3,6-Diaminoacridine is an acridine dye and also a DNA intercalator.

For research use only. We do not sell to patients.

3,6-Diaminoacridine Chemical Structure

CAS No. : 92-62-6

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of 3,6-Diaminoacridine:

3,6-Diaminoacridine hemisulfate In-stock
3,6-Diaminoacridine dihydrochloride Get quote
3,6-Diaminoacridine hydrochloride, 98% Get quote

3 Publications Citing Use of MCE 3,6-Diaminoacridine

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•Related Small Molecules:

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RNASE L DNA Polymerases RNA Polymerases Helicases DNA Ligase

Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A2780	IC₅₀	0.47 μM Compound: Acridine-3,6-diamine	Concentration required to inhibit the cell growth by 50 % after 96 hr A2780 leukemic cells. Concentration required to inhibit the cell growth by 50 % after 96 hr A2780 leukemic cells.	[PMID: 7658436]
A2780	IC₅₀	0.47 μM Compound: Proflavine	Compound was evaluated for cytotoxicity against A2780 cell lines. Compound was evaluated for cytotoxicity against A2780 cell lines.	10.1016/0960-894X(96)00130-8
A2780	IC₅₀	0.47 μM Compound: Proflavine	Cytotoxic potency required to inhibit A2780 cell growth by 50% after cell drug contact for 96 hrs Cytotoxic potency required to inhibit A2780 cell growth by 50% after cell drug contact for 96 hrs	[PMID: 9371240]
A549	CC₅₀	3.1 μM Compound: 23	Cytotoxicity against human A549 cells expressing ACE2 assessed as cell viability Cytotoxicity against human A549 cells expressing ACE2 assessed as cell viability	[PMID: 35620927]
CHO	IC₅₀	0.52 μM Compound: 1	Phototoxicity in transformed CHO cells after 24 hrs of UV irradiation by WST1 assay Phototoxicity in transformed CHO cells after 24 hrs of UV irradiation by WST1 assay	[PMID: 19217190]
CHO	IC₅₀	1.1 μM Compound: 1	Phototoxicity in transformed CHO cells after 24 hrs incubation in dark by WST1 assay Phototoxicity in transformed CHO cells after 24 hrs incubation in dark by WST1 assay	[PMID: 19217190]
Keratinocyte	IC₅₀	> 50 μM Compound: 1	Phototoxicity in normal human keratinocytes after 24 hrs incubation in dark by WST1 assay Phototoxicity in normal human keratinocytes after 24 hrs incubation in dark by WST1 assay	[PMID: 19217190]
Keratinocyte	IC₅₀	> 50 μM Compound: 1	Phototoxicity in normal human keratinocytes after 24 hrs of UV irradiation by WST1 assay Phototoxicity in normal human keratinocytes after 24 hrs of UV irradiation by WST1 assay	[PMID: 19217190]
L1210	IC₅₀	2.7 μM Compound: Acridine-3,6-diamine	Concentration required to inhibit the cell growth by 50 % after 48 hr in L1210 leukemic cells. Concentration required to inhibit the cell growth by 50 % after 48 hr in L1210 leukemic cells.	[PMID: 7658436]
L1210	IC₅₀	2.7 μM Compound: Proflavine	Cytotoxic potency required to inhibit L1210 cell growth by 50% after cell drug contact for 48 hrs Cytotoxic potency required to inhibit L1210 cell growth by 50% after cell drug contact for 48 hrs	[PMID: 9371240]
SK-MEL-2	IC₅₀	1.6 μM Compound: Proflavine	Cytotoxicity against human SK-MEL-2 cells expressing NRAS mutant assessed as cell growth inhibition incubated for 48 hrs by MTT assay Cytotoxicity against human SK-MEL-2 cells expressing NRAS mutant assessed as cell growth inhibition incubated for 48 hrs by MTT assay	[PMID: 36961300]
SK-MEL-2	IC₅₀	5.31 μM Compound: Proflavine	Cytotoxicity against human SK-MEL-2 cells assessed as cell growth inhibition incubated for 24 hrs by MTT assay Cytotoxicity against human SK-MEL-2 cells assessed as cell growth inhibition incubated for 24 hrs by MTT assay	[PMID: 36961300]
THP-1	IC₅₀	2.21 μM Compound: proflavine	Cytotoxicity against human THP1 cells Cytotoxicity against human THP1 cells	[PMID: 17418916]
Vero	CC₅₀	3.4 μM Compound: 23	Cytotoxicity against African green monkey Vero cells assessed as cell viability Cytotoxicity against African green monkey Vero cells assessed as cell viability	[PMID: 35620927]

In Vitro

3,6-Diaminoacridine induces cytotoxicity in transformed Chinese hamster ovary cells, with an IC₅₀ of 1.1 μM^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

209.25

Formula

C₁₃H₁₁N₃

CAS No.

92-62-6

Appearance

Solid

Color

Light yellow to yellow

SMILES

NC1=CC2=NC3=CC(N)=CC=C3C=C2C=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (597.37 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.7790 mL	23.8949 mL	47.7897 mL
5 mM	0.9558 mL	4.7790 mL	9.5579 mL
10 mM	0.4779 mL	2.3895 mL	4.7790 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

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Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.78%

Select Batch:

Data Sheet (271 KB) SDS (393 KB)

COA (244 KB) HNMR (230 KB) LCMS (83 KB)

Handling Instructions (2659 KB)

References

[1]. Kubota Y, et al. Fluorescence decay studies of the DNA-3,6-diaminoacridine complexes. Biophys Chem. 1984;19(1):25-37. [Content Brief]

[2]. Gamage SA, et al. Synthesis and in vitro evaluation of 9-anilino-3,6-diaminoacridines active against a multidrug-resistant strain of the malaria parasite Plasmodium falciparum. J Med Chem. 1994;37(10):1486-1494. [Content Brief]

[3]. Sabolova D, et al. Proflavine/acriflavine derivatives with versatile biological activities. J Appl Toxicol. 2020;40(1):64-71. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.7790 mL	23.8949 mL	47.7897 mL	119.4743 mL
	5 mM	0.9558 mL	4.7790 mL	9.5579 mL	23.8949 mL
	10 mM	0.4779 mL	2.3895 mL	4.7790 mL	11.9474 mL
	15 mM	0.3186 mL	1.5930 mL	3.1860 mL	7.9650 mL
	20 mM	0.2389 mL	1.1947 mL	2.3895 mL	5.9737 mL
	25 mM	0.1912 mL	0.9558 mL	1.9116 mL	4.7790 mL
	30 mM	0.1593 mL	0.7965 mL	1.5930 mL	3.9825 mL
	40 mM	0.1195 mL	0.5974 mL	1.1947 mL	2.9869 mL
	50 mM	0.0956 mL	0.4779 mL	0.9558 mL	2.3895 mL
	60 mM	0.0796 mL	0.3982 mL	0.7965 mL	1.9912 mL
	80 mM	0.0597 mL	0.2987 mL	0.5974 mL	1.4934 mL
	100 mM	0.0478 mL	0.2389 mL	0.4779 mL	1.1947 mL