Pseudoprotodioscin

Name: Pseudoprotodioscin
Brand: MedChemExpress
SKU: HY-N0686
Rating: 4.5 (1 reviews)

Cat. No.: HY-N0686 Purity: 98.76%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Pseudoprotodioscin, a furostanoside, inhibits SREBP1/2 and microRNA 33a/b levels and reduces the gene expression regarding the synthesis of cholesterol and triglycerides.

For research use only. We do not sell to patients.

Pseudoprotodioscin Chemical Structure

CAS No. : 102115-79-7

Size	Price	Stock	Quantity
1 mg	USD 47	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 99	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 168	In-stock	Estimated Time of Arrival: December 31
20 mg	USD 286	In-stock	Estimated Time of Arrival: December 31
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100 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE Pseudoprotodioscin

•Int J Mol Sci. 2021 May 31;22(11):5951. [Abstract]

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Pseudoprotodioscin, a furostanoside, inhibits SREBP1/2 and microRNA 33a/b levels and reduces the gene expression regarding the synthesis of cholesterol and triglycerides^[1].

In Vitro

In Hep G2 cells, Pseudoprotodioscin increases ABCA1 protein and mRNA levels, and promotes the effluxion of ApoA-1-mediated cholesterol. Pseudoprotodioscin inhibits SREBP1c and SREBP2 transcription by decreasing microRNA 33a/b levels. This procedure reciprocally lead to the increase of ABCA1 levels. In THP-1 macrophages, Pseudoprotodioscin shows the similar effect, which reduces HMGCR, FAS and ACC mRNA levels and promotes low density lipoprotein receptor by decreasing the PCSK9 levels^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

1031.18

Formula

C₅₁H₈₂O₂₁

CAS No.

102115-79-7

Appearance

Solid

Color

Off-white to light yellow

SMILES

C[C@@]1([C@](C(C)=C(CC[C@@H](C)CO[C@@H]([C@@H]([C@@H](O)[C@@H]2O)O)O[C@@H]2CO)O3)([H])[C@]3([H])C4)[C@]4([H])[C@@](CC=C5[C@@]6(CC[C@H](O[C@@](O[C@H](CO)[C@@H](O[C@@](O[C@@H](C)[C@H](O)[C@H]7O)([H])[C@@H]7O)[C@@H]8O)([H])[C@@H]8O[C@@](O[C@@H](C)[C@H](O)[C@H]9O)([H])[C@@H]9O)C5)C)([H])[C@]6([H])CC1

Structure Classification

Others

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

H₂O : ≥ 100 mg/mL (96.98 mM)

DMSO : 100 mg/mL (96.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.9698 mL	4.8488 mL	9.6976 mL
5 mM	0.1940 mL	0.9698 mL	1.9395 mL
10 mM	0.0970 mL	0.4849 mL	0.9698 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (2.42 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (2.42 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (2.42 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 98.76%

Select Batch:

Data Sheet (275 KB) SDS (251 KB)

COA (252 KB) HNMR (279 KB) RP-HPLC (123 KB) MS (82 KB)

Handling Instructions (2659 KB)

References

[1]. Gai Y, et al. Pseudoprotodioscin inhibits SREBPs and microRNA 33a/b levels and reduces the gene expression regarding the synthesis of cholesterol and triglycerides. Fitoterapia. 2019 Nov;139:104393. [Content Brief]

[2]. Liang ZZ, et al. Oligofurostanosides from Asparagus cochinchinensis. Planta Med. 1988 Aug;54(4):344-6. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	0.9698 mL	4.8488 mL	9.6976 mL	24.2441 mL
	5 mM	0.1940 mL	0.9698 mL	1.9395 mL	4.8488 mL
	10 mM	0.0970 mL	0.4849 mL	0.9698 mL	2.4244 mL
	15 mM	0.0647 mL	0.3233 mL	0.6465 mL	1.6163 mL
	20 mM	0.0485 mL	0.2424 mL	0.4849 mL	1.2122 mL
	25 mM	0.0388 mL	0.1940 mL	0.3879 mL	0.9698 mL
	30 mM	0.0323 mL	0.1616 mL	0.3233 mL	0.8081 mL
	40 mM	0.0242 mL	0.1212 mL	0.2424 mL	0.6061 mL
	50 mM	0.0194 mL	0.0970 mL	0.1940 mL	0.4849 mL
	60 mM	0.0162 mL	0.0808 mL	0.1616 mL	0.4041 mL
	80 mM	0.0121 mL	0.0606 mL	0.1212 mL	0.3031 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.