Denifanstat (Synonyms: TVB-2640; FASN-IN-2; ASC-40)

Name: Denifanstat
Brand: MedChemExpress
SKU: HY-112829
Rating: 5.0 (7 reviews)

Cat. No.: HY-112829 Purity: 99.58%: Data Sheet
COA

SDS
Handling Instructions
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Denifanstat (TVB-2640) is an orally active and potent Fatty Acid Synthase (FASN) inhibitor with an IC₅₀ of 0.052 μM and an EC₅₀ of 0.072 μM. Denifanstat has the potential for fatty liver disease and cancer research.

For research use only. We do not sell to patients.

CAS No. : 1399177-37-7

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 7 publication(s) in Google Scholar

7 Publications Citing Use of MCE Denifanstat

•bioRxiv. 2024 August 23.

In Vivo Efficacy Study

RT-PCR

: Denifanstat purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2025 Aug;45(8):1010-1037. [Abstract]; Denifanstat (10 mg/kg; i.g.; every day; from day7 to day20); Lovastatin(10 mg/kg; i.g.; every day; from day7 to day20); Simvastatin (50 mg/kg; i.g.; every day; from day7 to day20). Tumor growth curve of each group (4 mice/group). ns, not significant; * P < 0.05, **P < 0.01, ***P < 0.001.

: Denifanstat purchased from MedChemExpress. Usage Cited in: Nat Metab. 2021 Nov;3(11):1466-1475. [Abstract]; Fatty acid synthase inhibitors suppress SARS-CoV-2 virus replication. Representative fluorescence images of SARS-CoV-2-mNG infected multiple cell lines. Indicated cells were pre-treated with Denifanstat (TVB-2640) (1-2 µM) at the following concentration: NCI-H1355 and NCI-1437 (2 µM); Caco-2 (2 µM); MEF-hACE2 (1 µM), from 16 h before infection and then infected with SARS-CoV-2-mNG at an MOI = 0.1(NCI-H1355, NCI-1437), MOI = 0.05 (Caco-2), MOI = 0.01 (MEF-hACE2). Fluorescence images were acquired at 48 hpi. Hoechst 33342 was used for nuclear staining. Scale bar: 200 μm.

: Denifanstat purchased from MedChemExpress. Usage Cited in: Nat Metab. 2021 Nov;3(11):1466-1475. [Abstract]; Palmitic acid reverses the effects of knockout and inhibition of fatty acid synthase on viral replication. Representative fluorescence images of palmitoylated protein assay. HEK293T-hACE2 and NCI-H1355 cells were treated with Denifanstat (TVB-2640)100 nM; NCI-H1355 cells: Denifanstat (TVB-2640) 2 µM for 24 h and the EZClick palmitic acid label was added to the medium for another 24 h. Cells were then processed and analyzed by microscopy following the manufacturer's instruction. Scale bar: 40 μm.

: Denifanstat purchased from MedChemExpress. Usage Cited in: Nat Metab. 2021 Nov;3(11):1466-1475. [Abstract]; Denifanstat (TVB-2640) reduces body weight loss and prolongs mouse survival after SARS-CoV-2 infection. K18-hACE2 transgenic mice were infected with SARS-CoV-2 virus and i.p. injected with TVB-2640 (8 mg/kg; i.p.; from day 0 to day 5.

: Denifanstat purchased from MedChemExpress. Usage Cited in: Nat Metab. 2021 Nov;3(11):1466-1475. [Abstract]; Fatty acid synthase inhibitors suppress the replication of SARS-CoV-2 variants. NCI-H1355 cells were pre-treated with Denifanstat (TVB-2640) (2 µM) for 16 h and infected with SARS-CoV-2 variants with an MOI of 0.1. The cells were analyzed by qPCR for viral RNA at 48 hpi.

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Description

IC₅₀ & Target

IC50: 0.052 μM (FASN)^[1]

In Vitro

Denifanstat (compound 152) is a potent FASN inhibitor^[2].
Fatty Acid Synthase (FASN) inhibition prevents palmitoylation of SARS-CoV2 spike protein^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

439.55

Formula

C₂₇H₂₉N₅O

CAS No.

1399177-37-7

Appearance

Solid

Color

White to off-white

SMILES

N#CC1=CC=C(C2CCN(C(C3=CC(C4=NC(C)=NN4)=C(C5CCC5)C=C3C)=O)CC2)C=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (227.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2751 mL	11.3753 mL	22.7505 mL
5 mM	0.4550 mL	2.2751 mL	4.5501 mL
10 mM	0.2275 mL	1.1375 mL	2.2751 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.69 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.69 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.69 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.58%

Select Batch:

Data Sheet (272 KB) SDS (252 KB)

COA (247 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Minhyoung Lee, et al. Fatty Acid Synthase inhibition prevents palmitoylation of SARS-CoV2 Spike Protein and improves survival of mice infected with murine hepatitis virus. BioRxiv, December 21, 2020.

[2]. Johan D., et al. Heterocyclic modulators of lipid synthesis. WO2012122391A1.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.2751 mL	11.3753 mL	22.7505 mL	56.8764 mL
	5 mM	0.4550 mL	2.2751 mL	4.5501 mL	11.3753 mL
	10 mM	0.2275 mL	1.1375 mL	2.2751 mL	5.6876 mL
	15 mM	0.1517 mL	0.7584 mL	1.5167 mL	3.7918 mL
	20 mM	0.1138 mL	0.5688 mL	1.1375 mL	2.8438 mL
	25 mM	0.0910 mL	0.4550 mL	0.9100 mL	2.2751 mL
	30 mM	0.0758 mL	0.3792 mL	0.7584 mL	1.8959 mL
	40 mM	0.0569 mL	0.2844 mL	0.5688 mL	1.4219 mL
	50 mM	0.0455 mL	0.2275 mL	0.4550 mL	1.1375 mL
	60 mM	0.0379 mL	0.1896 mL	0.3792 mL	0.9479 mL
	80 mM	0.0284 mL	0.1422 mL	0.2844 mL	0.7110 mL
	100 mM	0.0228 mL	0.1138 mL	0.2275 mL	0.5688 mL

Denifanstat Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Denifanstat1399177-37-7TVB-2640 FASN-IN-2 ASC-40TVB2640TVB 2640ASC40ASC 40ASC-40Fatty Acid Synthase (FASN)SARS-CoV2spikeproteinCOVID-19Inhibitorinhibitorinhibit

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Denifanstat (Synonyms: TVB-2640; FASN-IN-2; ASC-40)

Customer Review

Denifanstat Related Antibodies

7 Publications Citing Use of MCE Denifanstat

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Denifanstat Related Antibodies

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Complete Stock Solution Preparation Table

Denifanstat Related Classifications

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