2. GPCR/G Protein MAPK/ERK Pathway
  3. Ras
  4. (R)-Ketorolac

(R)-Ketorolac  (Synonyms: (+)-Ketorolac)

Cat. No.: HY-B0580B Purity: 99.65%
COA
SDS
Handling Instructions Technical Support

(R)-Ketorolac is an orally active Cdc42 and Rac1 inhibitor. (R)-Ketorolac inhibits GTPase. (R)-Ketorolac alters ovarian cancer cell behaviors critical for invasion and metastasis. (R)-Ketorolac ameliorates cancer-associated cachexia.

For research use only. We do not sell to patients.

CAS No. : 66635-93-6

Size Price Stock Quantity
Free Sample (0.1 - 0.2 mg)   Apply Now  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
5 mg In-stock
10 mg In-stock
25 mg In-stock
50 mg In-stock
100 mg In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of (R)-Ketorolac:

(R)-Ketorolac Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE (R)-Ketorolac

Flow Cytometry

    (R)-Ketorolac purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2023 Dec;299(12):105377.  [Abstract]

    R-Ketorolac (1-100 μM; 48 h). MDA-MB-231 cells are cultured in poly-HEMA-coated dishes in the presence or absence of R-Ketorolac for 48 h, and the cells are collected for anoikis analysis by Annexin V/PI staining. Control and 1 μM R-Ketorolac (R-Ket)-treated groups are used for quantification, and graph data are presented as mean ± SD (n = 3).

    View All Ras Isoform Specific Products:

    View All Isoforms
    K-Ras H-Ras N-Ras Ras

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    (R)-Ketorolac is an orally active Cdc42 and Rac1 inhibitor. (R)-Ketorolac inhibits GTPase. (R)-Ketorolac alters ovarian cancer cell behaviors critical for invasion and metastasis. (R)-Ketorolac ameliorates cancer-associated cachexia[1][2][3].

    In Vitro

    (R)-Ketorolac substantially inhibits growth factor dependent activation of Cdc42 and Rac1 in a dose-dependent manner with an EC50 value of 2.577 μM for Cdc42 and 0.587 μM for Rac1 in SKOV3ip cells[2].
    (R)-Ketorolac (1-10 μM) decreases numbers length of filopodia in SKOV3ip cells and reduces SKOV3ip cells adherence to both fibronectin and collagen[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    (R)-Ketorolac Related Antibodies
    In Vivo

    (R)-Ketorolac (2 mg/kg, p.o., daily) ameliorates cancer-associated cachexia and prolongs survival of C26 tumour-bearing mice[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: C26 tumour-bearing mice[3]
    Dosage: 2 mg/kg
    Administration: Oral gavage (p.o.), every day after cachexia onset
    Result: Prolonged survival and alleviates C26-induced weight loss independent of tumour growth and food intake.
    Showed a tumour weight of 0.7 g and a weight loss of 0.61 g.
    Did not affect C26-associated hepatosplenomegaly and splenic lymphocyte numbers.
    Prevented C26-induced T-lymphopenia.
    Molecular Weight

    255.27

    Formula

    C15H13NO3
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(C1=CC=CC=C1)C2=CC=C3N2CC[C@H]3C(O)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 100 mg/mL (391.74 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.9174 mL 19.5871 mL 39.1742 mL
    5 mM 0.7835 mL 3.9174 mL 7.8348 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 5 mg/mL (19.59 mM); Clear solution

      This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 5 mg/mL (19.59 mM); Clear solution

      This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.65%

    SDS (393 KB)

    COA (255 KB) LCMS (92 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.9174 mL 19.5871 mL 39.1742 mL 97.9355 mL
    5 mM 0.7835 mL 3.9174 mL 7.8348 mL 19.5871 mL
    10 mM 0.3917 mL 1.9587 mL 3.9174 mL 9.7936 mL
    15 mM 0.2612 mL 1.3058 mL 2.6116 mL 6.5290 mL
    20 mM 0.1959 mL 0.9794 mL 1.9587 mL 4.8968 mL
    25 mM 0.1567 mL 0.7835 mL 1.5670 mL 3.9174 mL
    30 mM 0.1306 mL 0.6529 mL 1.3058 mL 3.2645 mL
    40 mM 0.0979 mL 0.4897 mL 0.9794 mL 2.4484 mL
    50 mM 0.0783 mL 0.3917 mL 0.7835 mL 1.9587 mL
    60 mM 0.0653 mL 0.3265 mL 0.6529 mL 1.6323 mL
    80 mM 0.0490 mL 0.2448 mL 0.4897 mL 1.2242 mL
    100 mM 0.0392 mL 0.1959 mL 0.3917 mL 0.9794 mL
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    (R)-Ketorolac Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    (R)-Ketorolac66635-93-6(+)-KetorolacRasovarian cancerInhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Requested Quantity *

    Applicant Name *

    		 

    Salutation

    Email Address *

    		 

    Phone Number *

    Department

    		 

    Organization Name *

    City

    State

    Country or Region *

    		      

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    (R)-Ketorolac
    Cat. No.:
    HY-B0580B
    Quantity:
    MCE Japan Authorized Agent:

    Customer Review

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Lot #

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Country or Region *

    Remarks

    SAFETY DATA SHEET (SDS)

    English - EN (393 KB) Français - FR (393 KB) Deutsch - DE (393 KB) Norwegian - NO (393 KB) Español - ES (393 KB) Swedish - SV (393 KB) Italian - IT (393 KB) Korean - KR (393 KB) Portuguese - PT (393 KB)

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.