RCC-36 hydrochloride 

RCC-36 hydrochloride is an L-type calcium channel inhibitor and competitive muscarinic receptor antagonist. RCC-36 hydrochloride inhibits L-type calcium currents in voltage- and concentration-dependent fashion with no effect on cardiac K⁺ currents. RCC-36 hydrochloride suppresses maximum acetylcholine-induced contractile responses, inhibits detrusor muscle contractions induced by potassium chloride, calcium chloride, and electric field stimulation, including atropine-resistant contractions. RCC-36 hydrochloride can be used for the research of urinary frequency, urinary incontinence, and bladder overactivity^[1][2][3].

RCC-36 (10 μM; 5 min) hydrochloride inhibits L-type calcium currents in isolated guinea pig detrusor smooth muscle cells in vitro in a voltage- and concentration-dependent manner, with an IC₅₀ of 6.4 µM at a holding potential of -60 mV and 1.2 µM at -40 mV, and decreases the calcium window current by shifting steady-state inactivation curves hyperpolarizingly without altering activation curves^[1].
RCC-36 (1-100 µM) hydrochloride potently inhibits L-type Ca²⁺ currents in isolated guinea pig ventricular myocytes with an IC₅₀ of 27.0 µM^[2].
RCC-36 (10 nM-3 μM; at least 30 minutes) hydrochloride exhibits competitive anticholinergic activity on isolated human detrusor smooth muscle, with a pA₂ value of 7.20, and suppresses the maximum acetylcholine-induced contraction at 3 μM^[3].
RCC-36 (1 μM-1 mM; at least 30 minutes) hydrochloride inhibits 80 mM KCl-induced contractions in isolated human detrusor smooth muscle with a maximum inhibition of 87.1% and an IC₅₀ of 13.0 μM^[3].
RCC-36 (100 nM-1 mM; at least 30 minutes) hydrochloride potently inhibits 5 mM CaCl₂-induced contractions in isolated human detrusor smooth muscle with a maximum inhibition of 98.5% and an IC₅₀ of 1.34 μM^[3].
RCC-36 (1 nM-200 μM; at least 30 minutes) hydrochloride inhibits electric field-induced contractions in isolated human detrusor smooth muscle with a maximum inhibition of 81.1% and an IC₅₀ of 2653 μM^[3].
RCC-36 (10 nM-300 μM; at least 30 minutes) hydrochloride potently inhibits Atropine (HY-B1205)-resistant electric field-induced contractions in isolated human detrusor smooth muscle with a maximum inhibition of 82.3% and an IC₅₀ of 2.65 μM^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

393.95

C₂₂H₃₂ClNO₃

129927-37-3

O=C(C(C1=CC=CC=C1)(C2CCCCC2)O)OC(C)(C)C#CCNCC.Cl

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Hayashi S, et al. Inhibitory effects of NS-21, a novel drug for urinary incontinence, and its active metabolite, RCC-36, on L-type calcium currents in isolated guinea pig detrusor smooth muscle cells. Naunyn Schmiedebergs Arch Pharmacol. 1997;355(5):659-666.

  [2]. Hayashi S, et al. Cardiac electrophysiological actions of NS-21 and its active metabolite, RCC-36, compared with terodiline. Naunyn Schmiedebergs Arch Pharmacol. 1997;355(5):651-658.

  [3]. Kikukawa H, et al. Pharmacologic actions of temiverine (p-INN) and its active metabolite, RCC-36, on isolated human urinary bladder muscle. Int J Urol. 1998;5(3):268-275.  [Content Brief]