LAMTOR1 Protein, Human (His)

The LAMTOR1 protein is an important component of the Ragulator complex and plays dual roles in amino acid sensing and mTORC1 activation. It acts as a guanine nucleotide exchange factor (GEF) for Rag GTPases and mediates their recruitment to the lysosomal membrane. LAMTOR1 Protein, Human (His) is the recombinant human-derived LAMTOR1 protein, expressed by E. coli , with N-His labeled tag. The total length of LAMTOR1 Protein, Human (His) is 160 a.a., with molecular weight of ~24 kDa.

LAMTOR1 stands as a crucial component within the Ragulator complex, a multifaceted assembly involved in amino acid sensing and the activation of mTORC1, a signaling nexus orchestrating cell growth in response to diverse stimuli, including growth factors, energy levels, and amino acids. Serving a dual role for the small GTPases Rag, the Ragulator acts as both a guanine nucleotide exchange factor (GEF) activating Rag GTPases and a mediator for their recruitment to the lysosome membrane. In this intricate interplay, activated Ragulator and Rag GTPases synergistically act as a scaffold, orchestrating the recruitment and activation of mTORC1 at lysosomes. LAMTOR1, pivotal to this mechanism, directly anchors the Ragulator complex to the lysosomal membrane, assuming a critical role in holding together other subunits of the Ragulator complex. Additionally, LAMTOR1's interaction with Rag GTPases plays a central role in the recruitment of the mTORC1 complex to lysosomes. Beyond its role in mTOR signaling, LAMTOR1 demonstrates its versatility by participating in processes such as the control of embryonic stem cell differentiation, regulation of late endosomes/lysosomes biogenesis, and potential involvement in cholesterol homeostasis and RHOA activation. As part of the Ragulator complex and various interacting networks, LAMTOR1 emerges as a key orchestrator in cellular responses to diverse stimuli, contributing to the regulation of essential cellular processes.

Human

E. coli

N-His

Q6IAA8 (2G-161P)

55004  [NCBI]

GCCYSSENEDSDQDREERKLLLDPSSPPTKALNGAEPNYHSLPSARTDEQALLSSILAKTASNIIDVSAADSQGMEQHEYMDRARQYSTRLAVLSSSLTHWKKLPPLPSLTSQPHQVLASEPIPFSDLQQVSRIAAYAYSALSQIRVDAKEELVVQFGIP

Approximately 24 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized after extensive dialysis against solution in PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.