2. Metabolic Enzyme/Protease NF-κB Immunology/Inflammation
  3. Epoxide Hydrolase Reactive Oxygen Species (ROS) Interleukin Related NF-κB
  4. sEH-IN-23

sEH-IN-23 is a soluble epoxide hydrolase inhibitor with a IC50 of 0.8 nM against human sEH and 0.7 nM against murine sEH. sEH-IN-23 inhibits inflammatory factor production mediated by NF-κB activation, reactive oxygen species (ROS) generation, and the release of pro-inflammatory cytokines IL-1β, IL-6 and TNF-α. sEH-IN-23 exhibits anti-inflammatory activity in acute lung injury models. sEH-IN-23 can be used for the research of acute lung injury.

For research use only. We do not sell to patients.

sEH-IN-23

sEH-IN-23 Chemical Structure

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sEH-IN-23 Related Antibodies

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Description

sEH-IN-23 is a soluble epoxide hydrolase inhibitor with a IC50 of 0.8 nM against human sEH and 0.7 nM against murine sEH. sEH-IN-23 inhibits inflammatory factor production mediated by NF-κB activation, reactive oxygen species (ROS) generation, and the release of pro-inflammatory cytokines IL-1β, IL-6 and TNF-α. sEH-IN-23 exhibits anti-inflammatory activity in acute lung injury models. sEH-IN-23 can be used for the research of acute lung injury[1].

IC50 & Target[1]

EH1

 

IL-6

 

IL-1β

 

In Vitro

sEH-IN-23 (compound 20K) (100 μg/mL; 0-60 min) has a half-life of 189.00 min in in vitro rat liver microsome stability assays[1].
Following incubation for 48 h in the CCK-8 assay, sEH-IN-23 (12.5-100 μM; 48 h) maintains the viability of RAW264.7 macrophages above 75%[1].
sEH-IN-23 (10-20 μM; 1-2 h pre-incubation) dose-dependently inhibits LPS (HY-D1056)-induced M1 polarization, reduces the release of IL-6 and TNF-α, and decreases ROS production in RAW264.7 macrophages[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

sEH-IN-23 Related Antibodies

Cell Viability Assay[1]

Cell Line: RAW264.7 macrophages
Concentration: 12.5, 25, 50, 100 μM
Incubation Time: 48 h
Result: Maintained cell viability higher than 75% at all tested concentrations.
Parmacokinetics
Species Dose Route Tmax Cmax T1/2 CL Vz AUC0-12 AUC0-∞ F
Rat[1] 8 mg/kg i.v. 0.12 h 14.38 μM 9.77 h 0.33 L/h 4.68 L 12.11 μM·h 24.07 μM·h 125.9 %
Rat[1] 50 mg/kg i.p. 3.00 h 35.35 μM 1.09 h 0.27 L/h 0.44 L 186.87 μM·h 189.49 μM·h /
In Vivo

sEH-IN-23 (15-30 mg/kg; i.p.; once daily; for 5 consecutive days) exhibits dose-dependent anti-inflammatory and antioxidant activities in a mouse model of LPS (HY-D1056)-induced acute lung injury[1].
sEH-IN-23 (2 g/kg; p.o.; daily; for 14 consecutive days) is well tolerated in mice, with no toxicity observed in monitoring indicators or histopathology of major organs[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 (male, 20-22 g, intraperitoneal injection of LPS)[1]
Dosage: 15 mg/kg; 30 mg/kg
Administration: i.p.; once daily; 5 consecutive days
Result: Reduced lung histopathology scores.
Alleviated alveolar obstruction, alveolar wall thickening, and neutrophil exudation in lung tissue.
Inhibited serum inflammatory cytokines in a dose-dependent manner, reduced levels of IL-1β, IL-6, and TNF-α.
Reduced CD68-positive macrophage infiltration in lung tissue.
Inhibited LPS-induced nuclear translocation of p65 (NF-κB pathway component) in lung tissue and in F4/80-marked lung macrophages.
Reduced lung tissue myeloperoxidase (MPO) activity in a dose-dependent manner.
Reduced reactive oxygen species (ROS) levels in lung tissue.
Molecular Weight

514.88

Formula

C22H22ClF3N4O5
SMILES

O=C(O)C1=CC=C(CNC(N2CCC(NC(NC3=CC=C(OC(F)(F)F)C(Cl)=C3)=O)CC2)=O)C=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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sEH-IN-23 Related Classifications

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

sEH-IN-23Epoxide HydrolaseReactive Oxygen Species (ROS)Interleukin RelatedNF-κBILNuclear factor-κBNuclear factor-kappaBmyeloperoxidaseacute lung injuryreactive oxygen speciesRAW264.7 macrophagesIL-1βM1 macrophage polarizationIL-6soluble epoxide hydrolaseTNF-αInhibitorinhibitorinhibit

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