1. Apoptosis
  2. Apoptosis
  3. Sophoridine

Sophoridine 

Cat. No.: HY-N1373 Purity: >98.0%
Handling Instructions

Sophoridine is a quinolizidine alkaloid isolated from leafs of Leguminous plant Sophora alopecuroides.L. Sophoridine induces apoptosis. Sophoridine has the potential to be a novel, potent and selective antitumor drug candidate for pancreatic cancer with well-tolerated toxicity.

For research use only. We do not sell to patients.

Sophoridine Chemical Structure

Sophoridine Chemical Structure

CAS No. : 6882-68-4

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10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
5 mg USD 50 In-stock
Estimated Time of Arrival: December 31
10 mg USD 80 In-stock
Estimated Time of Arrival: December 31
20 mg USD 140 In-stock
Estimated Time of Arrival: December 31
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Description

Sophoridine is a quinolizidine alkaloid isolated from leafs of Leguminous plant Sophora alopecuroides.L. Sophoridine induces apoptosis. Sophoridine has the potential to be a novel, potent and selective antitumor drug candidate for pancreatic cancer with well-tolerated toxicity[1].

In Vitro

Sophoridine (0-500 μM; 48 hours) exhibits remarkable inhibition effects to the growth of human pancreatic, gastric, liver, colon, gallbladder, and prostate carcinoma cells with IC50 values of about 20 μM to 200 μM[1].
Sophoridine (0-20 μM; 48 hours) increases S phase cell population from 26.23% (control) to 38.67% in Miapaca-2 cells and from 29.56% (control) to 39.16% in PANC-1 cells, about a 1.5-fold and a 1.3-fold increase, respectively[1].
Sophoridine (0-20 μM; 48 hours) significantly increases bad and bax levels, and decreases bcl-2 and bcl-xl levels in contrast, with a significant increase in Bax/Bcl-2 ratio[1].

Cell Viability Assay[1]

Cell Line: Normal cells: IOSE144, HL-7702 and LO2, BEAS-2B, GES-1, HEK 293 T, HPDE, FHC, Human cancer cells: PANC-1, Mapaca-1, hepG2, SGC-7901, CBC-SD,SGC-996,PC-3,MKN-45,MGC-803,Hela and HCT116 cells
Concentration: 0, 3.9, 7.8, 15.5, 31, 62.5, 125, 250, 500 μM
Incubation Time: 48 hours
Result: Exhibited the most potent cytotoxicity to cancer cells.

Cell Cycle Analysis[1]

Cell Line: PANC-1 cells; Miapca-2 cells
Concentration: 20 μM
Incubation Time: 48 hours
Result: Led to accumulated population in the S phase.

Western Blot Analysis[1]

Cell Line: PANC-1 cells; Miapca-2 cells
Concentration: 20 μM
Incubation Time: 48 hours
Result: Induced the activation of intrinsic apoptosis pathway.
In Vivo

Sophoridine (intraperitoneal injection; 20 or 40 mg/kg; 21 days) can inhibit the growth of xenograft pancreatic tumors[1].

Animal Model: BALB/c homozygous (nu/nu) nude mice[1]
Dosage: 20 or 40 mg/kg
Administration: Intraperitoneal injection; 20 or 40 mg/kg; 21 days
Result: Decreased xenograft pancreatic tumors mass.
Molecular Weight

248.36

Formula

C₁₅H₂₄N₂O

CAS No.

6882-68-4

SMILES

O=C1CCC[[email protected]]2([H])[[email protected]@]3([H])CCCN4[[email protected]@]3([H])[[email protected]@](CCC4)([H])CN21

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (201.32 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.0264 mL 20.1321 mL 40.2641 mL
5 mM 0.8053 mL 4.0264 mL 8.0528 mL
10 mM 0.4026 mL 2.0132 mL 4.0264 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.17 mg/mL (8.74 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.17 mg/mL (8.74 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.17 mg/mL (8.74 mM); Clear solution

*All of the co-solvents are provided by MCE.
References

Purity: >98.0%

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Keywords:

SophoridineApoptosisInhibitorinhibitorinhibit

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