Cat. No.: HY-N10472

STAT3-IN-14

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Based on 1 Customer Validation

STAT3-IN-14 (Compound 1) is a STAT3 inhibitor and has STAT3 phosphorylation inhibitory activity. STAT3-IN-14 (Compound 1) can directly bind to the hinge region of STAT3.

For research use only. We do not sell to patients.

Purity: 95.89%
CAS No.: 123297-90-5
Formula: C₁₄H₁₀O₅
Molecular Weight:258.23

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

STAT3-IN-14 Chemical Structure

CAS No.: 123297-90-5

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Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

STAT3-IN-14 (Compound 1) is a STAT3 inhibitor and has STAT3 phosphorylation inhibitory activity. STAT3-IN-14 (Compound 1) can directly bind to the hinge region of STAT3^[1].

IC₅₀ & Target

IC50: 0.42 μM (MDA-MB-231); 0.50 μM (MCF7); 0.13 μM (A549) ^[1]

All STAT Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	EC₅₀	0.78 μM Compound: 1	Antiproliferative activity against human A549 cells after 72 hrs by trypan blue exclusion assay Antiproliferative activity against human A549 cells after 72 hrs by trypan blue exclusion assay	[PMID: 19674905]
A549	EC₅₀	0.96 μM Compound: 1	Cytotoxicity against human A549 cells assessed as cell viability by tryptan blue staining method Cytotoxicity against human A549 cells assessed as cell viability by tryptan blue staining method	[PMID: 17950604]
A549	IC₅₀	0.13 μM Compound: 1	Cytotoxicity against human A549 cells assessed as reduction in cell proliferation measured after 72 hrs by WST8 assay Cytotoxicity against human A549 cells assessed as reduction in cell proliferation measured after 72 hrs by WST8 assay	[PMID: 32044231]
A549	IC₅₀	0.16 μM Compound: 1; NQ801	Antiproliferative activity against human A549 cells assessed as inhibition of cell growth measured after 72 hrs by WST-8 method Antiproliferative activity against human A549 cells assessed as inhibition of cell growth measured after 72 hrs by WST-8 method	[PMID: 37343498]
Fibroblast	EC₅₀	11.1 μM Compound: 1	Antiproliferative activity against human skin fibroblast after 72 hrs by trypan blue exclusion assay Antiproliferative activity against human skin fibroblast after 72 hrs by trypan blue exclusion assay	[PMID: 19674905]
Hepatocyte	EC₅₀	11.1 μM Compound: 1	Antiproliferative activity against human hepatocytes after 72 hrs by trypan blue exclusion assay Antiproliferative activity against human hepatocytes after 72 hrs by trypan blue exclusion assay	[PMID: 19674905]
MCF7	EC₅₀	0.51 μM Compound: 1	Antiproliferative activity against human MCF7 cells after 72 hrs by trypan blue exclusion assay Antiproliferative activity against human MCF7 cells after 72 hrs by trypan blue exclusion assay	[PMID: 19674905]
MCF7	EC₅₀	3.5 μM Compound: 1	Cytotoxicity against human MCF7 cells assessed as cell viability by tryptan blue staining method Cytotoxicity against human MCF7 cells assessed as cell viability by tryptan blue staining method	[PMID: 17950604]
MCF7	IC₅₀	0.5 μM Compound: 1	Cytotoxicity against human MCF7 cells assessed as reduction in cell proliferation measured after 72 hrs by WST8 assay Cytotoxicity against human MCF7 cells assessed as reduction in cell proliferation measured after 72 hrs by WST8 assay	[PMID: 32044231]
MCF7	IC₅₀	2 μM Compound: 1	Inhibition of IL-6 induced STAT3 phosphorylation at Tyr705 residue in human MCF7 cells preincubated for 1 hr followed by IL-6 stimulation and measured after 1 hr by Western blot analysis Inhibition of IL-6 induced STAT3 phosphorylation at Tyr705 residue in human MCF7 cells preincubated for 1 hr followed by IL-6 stimulation and measured after 1 hr by Western blot analysis	[PMID: 32044231]
MDA-MB-231	IC₅₀	0.35 μM Compound: 1; NQ801	Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth measured after 72 hrs by WST-8 method Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth measured after 72 hrs by WST-8 method	[PMID: 37343498]
MDA-MB-231	IC₅₀	0.41 μM Compound: 1	Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell proliferation measured after 72 hrs by WST8 assay Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell proliferation measured after 72 hrs by WST8 assay	[PMID: 32044231]
PC-3	EC₅₀	0.14 μM Compound: 1	Cytotoxicity against human PC3 cells assessed as cell viability by tryptan blue staining method Cytotoxicity against human PC3 cells assessed as cell viability by tryptan blue staining method	[PMID: 17950604]
PC-3	EC₅₀	0.14 μM Compound: 1	Antiproliferative activity against human PC3 cells after 72 hrs by trypan blue exclusion assay Antiproliferative activity against human PC3 cells after 72 hrs by trypan blue exclusion assay	[PMID: 19674905]
Raji	IC₅₀	33.2 μM Compound: 1	Inhibition of 12-O-tetradecanoylphorbol-13-acetate-induced EBV-early antigen activation in human Raji cells after 48 hrs by immunofluorescence technique Inhibition of 12-O-tetradecanoylphorbol-13-acetate-induced EBV-early antigen activation in human Raji cells after 48 hrs by immunofluorescence technique	[PMID: 19674905]
Raji	IC₅₀	33.2 μM Compound: 1	Inhibition of TPA-induced EBV-EA activation in Raji cells Inhibition of TPA-induced EBV-EA activation in Raji cells	[PMID: 17950604]

In Vitro

STAT3-IN-14 (Compound 1) shows inhibitory activity against phosphor-STAT3.
STAT3-IN-14 (Compound 1) reduces the phosphorylation inhibitory activity against MDA-MB-231 cells (JAK/STAT pathway constitutively active) in does dependent manner, but no inhibitory activities against JAK kinases.
STAT3-IN-14 (Compound 1) has growth inhibitory activity with with IC₅₀ values of 0.42 μM, 0.50 μM and 0.13 μM in MDA-MB-231, MCF7 and A549cells, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

MCF7 cells; MDA-MB-231 cells
Concentration:

1 or 10 μM; 0.5, 1, 2, 4 μM
Incubation Time:

1 h; 2 h
Result:

Completely inhibited STAT3 phosphorylation at 10 μM, and partial inhibition at 1 μM.
Reduced the phosphorylation of STAT3 in does dependent manner.

Cell Proliferation Assay^[1]

Cell Line:

MDA-MB-231, MCF7 and A549 cells
Concentration:

0-30 μM
Incubation Time:

72 h
Result:

Showed moderate to strong growth inhibitory activity among the three cancer cell lines.

Chemical Information

CAS No. 123297-90-5
Appearance Solid
Molecular Weight 258.23
Formula C₁₄H₁₀O₅
Color Light brown to yellow
SMILES

O=C(C1=C2OC([C@@H](O)C)=C1)C3=C(C2=O)C=CC=C3O
Structure Classification
- Quinones
- Naphthalene Quinones
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 120 mg/mL (464.70 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.8725 mL

19.3626 mL

38.7252 mL

5 mM

0.7745 mL

3.8725 mL

7.7450 mL

10 mM

0.3873 mL

1.9363 mL

3.8725 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

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Dilution Calculator

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Teruyuki Tahara, et al. STAT3 inhibitory activity of naphthoquinones isolated from Tabebuia avellanedae. Bioorg Med Chem. 2020 Mar 15;28(6):115347. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.8725 mL	19.3626 mL	38.7252 mL	96.8129 mL
	5 mM	0.7745 mL	3.8725 mL	7.7450 mL	19.3626 mL
	10 mM	0.3873 mL	1.9363 mL	3.8725 mL	9.6813 mL
	15 mM	0.2582 mL	1.2908 mL	2.5817 mL	6.4542 mL
	20 mM	0.1936 mL	0.9681 mL	1.9363 mL	4.8406 mL
	25 mM	0.1549 mL	0.7745 mL	1.5490 mL	3.8725 mL
	30 mM	0.1291 mL	0.6454 mL	1.2908 mL	3.2271 mL
	40 mM	0.0968 mL	0.4841 mL	0.9681 mL	2.4203 mL
	50 mM	0.0775 mL	0.3873 mL	0.7745 mL	1.9363 mL
	60 mM	0.0645 mL	0.3227 mL	0.6454 mL	1.6135 mL
	80 mM	0.0484 mL	0.2420 mL	0.4841 mL	1.2102 mL
	100 mM	0.0387 mL	0.1936 mL	0.3873 mL	0.9681 mL

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STAT3-IN-14

Natural Product Library Plus

All STAT Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

STAT3-IN-14 Related Classifications

Cat. No.: HY-N10472