2. JAK/STAT Signaling Stem Cell/Wnt
  3. STAT
  4. STAT3-IN-15

STAT3-IN-15 is a potent and orally active STAT3 inhibitor against idiopathic pulmonary fibrosis (IPF). STAT3-IN-15 inhibits STAT3 phosphorylation. STAT3-IN-15 also inhibits the migration and deformation of epithelial cells induced by TGF-β1 and inhibit epithelial-mesenchymal transition (EMT).

For research use only. We do not sell to patients.

STAT3-IN-15 Chemical Structure

STAT3-IN-15 Chemical Structure

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 390 In-stock
Solution
10 mM * 1 mL in DMSO USD 390 In-stock
Solid
1 mg USD 190 In-stock
5 mg USD 420 In-stock
10 mg USD 630 In-stock
25 mg USD 1200 In-stock
50 mg USD 1800 In-stock
100 mg USD 2700 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

STAT3-IN-15 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE STAT3-IN-15

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STAT3 STAT5 STAT6 STAT1

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

STAT3-IN-15 is a potent and orally active STAT3 inhibitor against idiopathic pulmonary fibrosis (IPF). STAT3-IN-15 inhibits STAT3 phosphorylation. STAT3-IN-15 also inhibits the migration and deformation of epithelial cells induced by TGF-β1 and inhibit epithelial-mesenchymal transition (EMT)[1].

IC50 & Target[1]

STAT3

 

In Vitro

STAT3-IN-15 (compound 10k) inhibits NIH-3T3 cell proliferation with an IC50 of 0.47 μM[1].
STAT3-IN-15 forms hydrogen bonds with Lys591 and Ser636, occupying the pY subpocket of STAT3[1].
STAT3-IN-15 (0-100 nM, 72 h) inhibits fibroblast activation and proliferation[1].
STAT3-IN-15 (50 nM, 24 h) inhibited TGF-β1 (5 ng/mL)-induced activation of NIH-3T3 cells[1].
STAT3-IN-15 (200 nM, 24 h) blocks TGF-β1 induced EMT process in A549 cells, determined by morphological changes[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

STAT3-IN-15 Related Antibodies

Cell Viability Assay[1]

Cell Line: NIH-3T3 cells
Concentration: 0, 6.25, 12.5, 25, 50, 100 nM
Incubation Time: 72 h
Result: Inhibited NIH-3T3 cell viability dose-dependently.

Western Blot Analysis[1]

Cell Line: NIH-3T3 cells
Concentration: Inhibited NIH-3T3 cell viability dose-dependently.
Incubation Time: 24 h
Result: Inhibited the expression of α-SMA and collagen I and the phosphorylation of STAT3.
In Vivo

STAT3-IN-15 (compound 10k) (30 and 60 mg/kg, intragastric administration) alleviates Bleomycin.html" class="link-product" target="_blank"> Bleomycin (HY-108345)-induced pulmonary fibrosis in mouse[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BLM-induced pulmonary fibrosis mouse model[1]
Dosage: 30 and 60 mg/kg
Administration: Intragastric administration
Result: Recovered the lung structure and reduced the hydroxyproline content.
Reduced the expression of the p-Stat3Ty705 protein in the lung tissue.
Improved BLM-induced imbalance of immune microenvironment.
Molecular Weight

422.42

Formula

C20H17F3N2O3S
Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(N1CCN(CC1)CC2=CC(F)=C(C=C2F)F)C3=CC4=CC=CC=C4S3(=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (236.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3673 mL 11.8366 mL 23.6731 mL
5 mM 0.4735 mL 2.3673 mL 4.7346 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (5.92 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.3673 mL 11.8366 mL 23.6731 mL 59.1828 mL
5 mM 0.4735 mL 2.3673 mL 4.7346 mL 11.8366 mL
10 mM 0.2367 mL 1.1837 mL 2.3673 mL 5.9183 mL
15 mM 0.1578 mL 0.7891 mL 1.5782 mL 3.9455 mL
20 mM 0.1184 mL 0.5918 mL 1.1837 mL 2.9591 mL
25 mM 0.0947 mL 0.4735 mL 0.9469 mL 2.3673 mL
30 mM 0.0789 mL 0.3946 mL 0.7891 mL 1.9728 mL
40 mM 0.0592 mL 0.2959 mL 0.5918 mL 1.4796 mL
50 mM 0.0473 mL 0.2367 mL 0.4735 mL 1.1837 mL
60 mM 0.0395 mL 0.1973 mL 0.3946 mL 0.9864 mL
80 mM 0.0296 mL 0.1480 mL 0.2959 mL 0.7398 mL
100 mM 0.0237 mL 0.1184 mL 0.2367 mL 0.5918 mL
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STAT3-IN-15 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

STAT3-IN-15STATIdiopathic pulmonary fibrosisIPFNIH-3T3TGF-βInhibitorinhibitorinhibit

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Cat. No.:
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