STAT3-IN-15
Based on 2 publication(s) in Google Scholar
STAT3-IN-15 is a potent and orally active STAT3 inhibitor against idiopathic pulmonary fibrosis (IPF). STAT3-IN-15 inhibits STAT3 phosphorylation. STAT3-IN-15 also inhibits the migration and deformation of epithelial cells induced by TGF-β1 and inhibit epithelial-mesenchymal transition (EMT).
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit : 98.99%
- CAS. Nr.: 3037596-35-0
- Formel: C20H17F3N2O3S
- Molecular Weight:422.42
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Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) STAT3-IN-15
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Biologische Aktivität
Beschreibung
IC50 & Target
[1]|
STAT3 |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| NIH3T3 | IC50 |
0.47 μM
Compound: 10K
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Antiproliferative activity against mouse NIH3T3 cells assessed as cell growth inhibition incubated for 24 to 72 hrs by CCK-8 assay
Antiproliferative activity against mouse NIH3T3 cells assessed as cell growth inhibition incubated for 24 to 72 hrs by CCK-8 assay
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[PMID: 36463728] |
In Vitro
STAT3-IN-15 (compound 10k) inhibits NIH-3T3 cell proliferation with an IC50 of 0.47 μM[1].
STAT3-IN-15 forms hydrogen bonds with Lys591 and Ser636, occupying the pY subpocket of STAT3[1].
STAT3-IN-15 (0-100 nM, 72 h) inhibits fibroblast activation and proliferation[1].
STAT3-IN-15 (50 nM, 24 h) inhibited TGF-β1 (5 ng/mL)-induced activation of NIH-3T3 cells[1].
STAT3-IN-15 (200 nM, 24 h) blocks TGF-β1 induced EMT process in A549 cells, determined by morphological changes[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:NIH-3T3 cells
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Concentration:0, 6.25, 12.5, 25, 50, 100 nM
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Incubation Time:72 h
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Result:Inhibited NIH-3T3 cell viability dose-dependently.
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Cell Line:NIH-3T3 cells
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Concentration:Inhibited NIH-3T3 cell viability dose-dependently.
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Incubation Time:24 h
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Result:Inhibited the expression of α-SMA and collagen I and the phosphorylation of STAT3.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BLM-induced pulmonary fibrosis mouse model[1]
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Dosage:30 and 60 mg/kg
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Administration:Intragastric administration
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Result:Recovered the lung structure and reduced the hydroxyproline content.
Reduced the expression of the p-Stat3Ty705 protein in the lung tissue.
Improved BLM-induced imbalance of immune microenvironment.
Chemical Information
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CAS. Nr. 3037596-35-0
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Appearance Solid
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Molecular Weight 422.42
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Formel C20H17F3N2O3S
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Color Off-white to light yellow
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SMILES
O=C(N1CCN(CC1)CC2=CC(F)=C(C=C2F)F)C3=CC4=CC=CC=C4S3(=O)=O
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (2)
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Journal Impact Factor
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Most Recent
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iScience
Roquin-1 resolves sepsis-associated acute liver injury by regulating inflammatory profiles via miRNA cargo in extracellular vesicles. [Abstract]2023 Jul 12;26(8):107295. PMID: 37554446
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 100 mg/mL (236.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
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Data Sheet (277 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3673 mL | 11.8366 mL | 23.6731 mL | 59.1828 mL |
| 5 mM | 0.4735 mL | 2.3673 mL | 4.7346 mL | 11.8366 mL | |
| 10 mM | 0.2367 mL | 1.1837 mL | 2.3673 mL | 5.9183 mL | |
| 15 mM | 0.1578 mL | 0.7891 mL | 1.5782 mL | 3.9455 mL | |
| 20 mM | 0.1184 mL | 0.5918 mL | 1.1837 mL | 2.9591 mL | |
| 25 mM | 0.0947 mL | 0.4735 mL | 0.9469 mL | 2.3673 mL | |
| 30 mM | 0.0789 mL | 0.3946 mL | 0.7891 mL | 1.9728 mL | |
| 40 mM | 0.0592 mL | 0.2959 mL | 0.5918 mL | 1.4796 mL | |
| 50 mM | 0.0473 mL | 0.2367 mL | 0.4735 mL | 1.1837 mL | |
| 60 mM | 0.0395 mL | 0.1973 mL | 0.3946 mL | 0.9864 mL | |
| 80 mM | 0.0296 mL | 0.1480 mL | 0.2959 mL | 0.7398 mL | |
| 100 mM | 0.0237 mL | 0.1184 mL | 0.2367 mL | 0.5918 mL |
STAT3-IN-15 Related Classifications
HY-151976 Related Classifications
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