Cat. No.: HY-128588

STAT3-IN-3

7 Cited Publications

Customer Review

Based on 7 publication(s) in Google Scholar

STAT3-IN-3 is a potent and selective inhibitor of signal transducer and activator of transcription 3 (STAT3), with anti-proliferative activity. STAT3-IN-3 induces apoptosis in breast cancer cells. STAT3-IN-3 acts as a promising mitochondria-targeting STAT3 inhibitor for cancer research.

For research use only. We do not sell to patients.

Purity: 98.40%
CAS No.: 2361304-26-7
Formula: C₂₇H₂₆BrN₃O₆S
Molecular Weight:600.48

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

STAT3-IN-3 Chemical Structure

CAS No.: 2361304-26-7

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (7)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

STAT3

All STAT Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	IC₅₀	1.89 μM Compound: 7a	Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 31048139]
HepG2	IC₅₀	2.88 μM Compound: 7a	Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 31048139]
L02	IC₅₀	35.6 μM Compound: 7a	Cytotoxicity against human LO2 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human LO2 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 31048139]
MCF-10A	IC₅₀	14.62 μM Compound: 7a	Cytotoxicity against human MCF10A cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human MCF10A cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 31048139]
MCF7	IC₅₀	3.33 μM Compound: 7a	Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 31048139]
MDA-MB-231	IC₅₀	1.43 μM Compound: 7a	Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 31048139]

In Vitro

STAT3-IN-3 has no influence on the phosphorylation levels of STAT1, JAK2, Src and Erk1/2^[1].
? STAT3-IN-3 inhibits the growth of MDA-MB-231, HCT-116, HepG2, and MCF-7 cells with IC₅₀s of 1.43 μM, 1.89 μM, 2.88 μM, and 3.33 μM, respectively^[1].
? STAT3-IN-3 down-regulates the expression of STAT3 target genes Bcl-2 and Cyclin D1^[1].
? STAT3-IN-3 inhibits STAT3 tyrosine phosphorylation and serine phosphorylation^[1].
? STAT3-IN-3 inhibits STAT3 DNA-binding activity^[1].
? STAT3-IN-3 (1-4 μM; 24 hours) increases ROS production and remarkably reduces the mitochondrial membrane potential to induce mitochondrial apoptotic pathway^[1].
? STAT3-IN-3 (1-4 μM; 24 hours) can induce the cleavage of caspase-9, caspase-3 and PARP^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

MDA-MB-231 cells, HCT-116 cells, HepG2 cells, MCF-7cells
Concentration:

MTT assay
Incubation Time:

48 hours
Result:

Exhibited anti-proliferative activity.

Apoptosis Analysis^[1]

Cell Line:

MDA-MB-231 cells
Concentration:

0 μM, 1 μM, 2 μM, 4 μM
Incubation Time:

24 hours
Result:

Induced the apoptosis of MDA-MB-231 cells dose-dependently and the apoptosis rates.

Western Blot Analysis^[1]

Cell Line:

MDA-MB-231 cells
Concentration:

0 μM, 1 μM, 2 μM, 4 μM
Incubation Time:

24 hours
Result:

Induced the cleavage of caspase-9, caspase-3 and PARP.

In Vivo

STAT3-IN-3 (10mg/kg-20 mg/kg; i.p.; daily; for 14 days) possesses potent antitumor activity against implanted 4T1 breast tumors growth^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Adult female BALB/c mice (6 weeks of age)^[1]
Dosage:

10 mg/kg, 20 mg/kg
Administration:

Intraperitoneal injection, daily, for 14 days
Result:

Significantly inhibited tumor volume.

Chemical Information

CAS No. 2361304-26-7
Appearance Solid
Molecular Weight 600.48
Formula C₂₇H₂₆BrN₃O₆S
Color Light yellow to yellow
SMILES

BrC1=C2C(S(C(C(N3CCN(C(C4=CC5=CC=C(N(CC)CC)C=C5OC4=O)=O)CC3)=O)=C2)(=O)=O)=CC=C1
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (7)

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Journal Impact Factor

Cell Rep Med

STAT3 couples activated tyrosine kinase signaling to the oncogenic core transcriptional regulatory circuitry of anaplastic large cell lymphoma. [Abstract]

2024 Mar 19;5(3):101472. PMID: 38508140
Phytomedicine

Hypocrellin A against intrahepatic Cholangiocarcinoma via multi-target inhibition of the PI3K-AKT-mTOR, MAPK, and STAT3 signaling pathways. [Abstract]

2024 Sep 7:135:156022. PMID: 39284270
J Cell Mol Med

Exosomal HMGB1 Orchestrates NSCLC Progression and Immunosuppressive Macrophage Polarisation Through the TLR4/NF-κB/IL-6/STAT3 Signalling Cascade. [Abstract]

2026 Feb;30(3):e71050. PMID: 41645802
Sci Rep

Downregulation of CIAPIN1 regulates the proliferation, migration and glycolysis of breast cancer cells via inhibition of STAT3 pathway. [Abstract]

2024 Sep 5;14(1):20794. PMID: 39242716
Biochem Biophys Res Commun

Suppression of PAPP-A mitigates atherosclerosis by mediating macrophage polarization via STAT3 signaling. [Abstract]

2021 Mar 5:543:29-37. PMID: 33508770
Research Square Preprint

2021 Sep.
Research Square Preprint

2021 Apr.

Solvent & Solubility

In Vitro:

DMSO : 5 mg/mL (8.33 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.6653 mL

8.3267 mL

16.6533 mL

5 mM

0.3331 mL

1.6653 mL

3.3307 mL

10 mM

---

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Cai G, et al. Discovery of fluorescent coumarin-benzo[b]thiophene 1, 1-dioxide conjugates as mitochondria-targeting antitumor STAT3 inhibitors. Eur J Med Chem. 2019 Jul 15;174:236-251. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.6653 mL	8.3267 mL	16.6533 mL	41.6334 mL
DMSO	5 mM	0.3331 mL	1.6653 mL	3.3307 mL	8.3267 mL

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STAT3-IN-3

Apoptosis Compound Library

Immunology/Inflammation Compound Library

JAK/STAT Compound Library

Kinase Inhibitor Library

Stem Cell Signaling Compound Library

Anti-Cancer Compound Library

Small Molecule Immuno-Oncology Compound Library

Anti-Aging Compound Library

Covalent Screening Library

Differentiation Inducing Compound Library

Anti-Breast Cancer Compound Library

Anti-Lung Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Obesity Compound Library

Transcription Factor-Targeted Library

Anti-Liver Cancer Compound Library

Anti-Prostate Cancer Compound Library

Cancer Stem Cells Compound Library

Highly Selective Inhibitors Library

Highly Selective Activators Library

Cell Death Library

Anti-Hematopathy Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Covalent Screening Library Plus

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Lactylation Compound Library

Publications Citing Use of MedChemExpress (MCE) STAT3-IN-3

All STAT Isoforms