2. Metabolic Enzyme/Protease
  3. Phosphodiesterase (PDE)
  4. STF-1084

STF-1084 is a specific, cell-impermeable, competitive inhibitor of ENPP1 (Ki = 33 nM). STF-1084 increases extracellular cGAMP concentrations by preventing its degradation by ENPP1, thereby enhancing immune infiltration. STF-1084 acts synergistically with ionizing radiation (IR) and cGAMP to delay tumor progression. STF-1084 can be used to study cancers with low immunogenicity.

For research use only. We do not sell to patients.

STF-1084

STF-1084 Chemical Structure

CAS No. : 2298390-71-1

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
5 mg In-stock
10 mg In-stock
25 mg In-stock
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

STF-1084 Related Antibodies

Top Publications Citing Use of Products

View All Phosphodiesterase (PDE) Isoform Specific Products:

View All Isoforms
PDE1 PDE2 PDE3 PDE4 PDE5 PDE6 PDE7 PDE9 PDE10 PDE11 PDE12 PDE8 Autotaxin

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

STF-1084 is a specific, cell-impermeable, competitive inhibitor of ENPP1 (Ki = 33 nM). STF-1084 increases extracellular cGAMP concentrations by preventing its degradation by ENPP1, thereby enhancing immune infiltration. STF-1084 acts synergistically with ionizing radiation (IR) and cGAMP to delay tumor progression. STF-1084 can be used to study cancers with low immunogenicity[1].

In Vitro

STF-1084 (0.1-106 nM) inhibits extracellular cGAMP degradation (IC50 = 340 nM), but does not affect intracellular cGAMP in human ENPP1 transfected into 293T cGAS ENPP1−/− cells[1].

STF-1084 (10 μM) induces IFNB1 expression in CD14+ human ENPP1 transfected into 293T cGAS ENPP1−/− cells[1].

STF-1084 (50 μM, 48 h) increases extracellular cGAMP in 4T1-luc cells, E0771 cells, MC38 cells, MDA-MB-231 cells, MCF-7 cells and NMuMG cells via IR stimulation [1].

STF-1084 (1-106 nM) has low activity towards the ectonucleotidases alkaline phosphatase (Ki,app > 100 μM) and ENPP2 (Ki,app = 5.5 μM)[1].

STF-1084 (0.1-106 nM, 16 h) is non-toxic to primary human PBMCs[1].

STF-1084 (50 μM, 16 h) has no effect on cytokine production when cGAMP was electroporated into primary human PBMCs[1].

STF-1084 shows high stability (t1/2; 159 min) in both human and mouse liver microsomes[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

STF-1084 Related Antibodies
In Vivo

STF-1084 (100 μL of 1 mM, intratumoral injections, once, on day 0, 2, 4, and 7 after IR) increases expression of tumor-associated CD11c+ population, delays tumor progression synergized with IR and cGAMP in WT BALB/cJ mice bearing 4T1-luc cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: WT BALB/cJ mice (Five- to nine-week-old female mice) bearing 4T1-luc cells ((1x106) were orthotopically injected on day 0)[1]
Dosage: 100 μL of 1 mM
Administration: intratumoral injections, once, on day 0, 2, 4, and 7 after IR
Result: Increased expression of tumor-associated CD11c+ population, delayed tumor progression synergized with IR and cGAMP.
Molecular Weight

381.36

Formula

C17H24N3O5P
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OP(O)(CCC1CCN(C2=C3C=C(OC)C(OC)=CC3=NC=N2)CC1)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 12 mg/mL (31.47 mM; ultrasonic and adjust pH to 1 with 1 M HCl; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6222 mL 13.1110 mL 26.2219 mL
5 mM 0.5244 mL 2.6222 mL 5.2444 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.2 mg/mL (3.15 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.2 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 1.2 mg/mL (3.15 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.2 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.92%

SDS (615 KB)

COA (252 KB) HNMR (299 KB) RP-HPLC (254 KB) MS (72 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.6222 mL 13.1110 mL 26.2219 mL 65.5549 mL
5 mM 0.5244 mL 2.6222 mL 5.2444 mL 13.1110 mL
10 mM 0.2622 mL 1.3111 mL 2.6222 mL 6.5555 mL
15 mM 0.1748 mL 0.8741 mL 1.7481 mL 4.3703 mL
20 mM 0.1311 mL 0.6555 mL 1.3111 mL 3.2777 mL
25 mM 0.1049 mL 0.5244 mL 1.0489 mL 2.6222 mL
30 mM 0.0874 mL 0.4370 mL 0.8741 mL 2.1852 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

STF-1084 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

STF-10842298390-71-1STF1084STF 1084Phosphodiesterase (PDE)InhibitorPBMCs293T cGAS ENPP1?/? cellsWT BALB/cJ mice4T1-luc cellsinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
STF-1084
Cat. No.:
HY-141831
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (615 KB) Français - FR (615 KB) Deutsch - DE (615 KB) Norwegian - NO (615 KB) Español - ES (615 KB) Swedish - SV (615 KB) Italian - IT (615 KB) Korean - KR (615 KB) Portuguese - PT (615 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.