1. Anti-infection
  2. Fungal
  3. Swinholide A

Swinholide A 

Cat. No.: HY-111009
Handling Instructions

Swinholide A is the actin-binding marine polyketide and dimerizes actin with the Kd of ~ 50 nM. Swinholide A is a microfilament disrupting marine toxin that stabilizes actin dimers and severs actin filaments. Swinholide A disrupts the actin cytoskeleton of cells.Antifungal activity.

For research use only. We do not sell to patients.

Swinholide A Chemical Structure

Swinholide A Chemical Structure

CAS No. : 95927-67-6

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Description

Swinholide A is the actin-binding marine polyketide and dimerizes actin with the Kd of ~ 50 nM[1]. Swinholide A is a microfilament disrupting marine toxin that stabilizes actin dimers and severs actin filaments. Swinholide A disrupts the actin cytoskeleton of cells.Antifungal activity[2].

In Vitro

Swinholide A, first isolated from the Okinawan marine sponge Theonella swinhoei, dimerizes actin[1].
Swinholide A, isolated from the marien sponge Theonella swinhoei, is highly cytotoxic to a variety of cancer cell lines[2].
Swinholide A disrupts the actin cytoskeleton of cells grown in culture, sequesters actin dimers in vitro in both polymerizing and non-polymerizing buffers with a binding stoichiometry of one swinholide A molecule per actin dimer, and rapidly severs F-actin in vitro with high cooperativity[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[2]

Cell Line: Balb/c 3T3 and Swiss 3T3 cells
Concentration: 5-100 nM
Incubation Time: 1-24 h
Result: Exponentially growing cells exposed to 10 nM for 24 h became arborized with diffuse cytoplasmic staining and fluorescent punctate structures.
Partial cell retraction or arborization and diminution of microfilament bundles began after 2-4 h, with complete loss of stress fibers by 5-7 h at concentrations of 10-50 nM.
Caused rounding of cultured mouse embryo 3T3 fibroblast cells within 1 h at concentration of 80 nM.
Molecular Weight

1389.87

Formula

C78H132O20

CAS No.
SMILES

C[[email protected]]([[email protected]](OC(/C=C/C(C)=C/C[[email protected]@H](C[[email protected]@](C=CC1)([H])O[[email protected]]1([H])C[[email protected]@H]([[email protected]]([[email protected]](C[[email protected]]([[email protected]@H]2C)O)O)C)OC)O)=O)([H])[[email protected]]([[email protected]@H](C[[email protected]@H]([[email protected]@H]([[email protected]](C[[email protected]@]3([H])O[[email protected]@](C[[email protected]](C/C=C(/C=C/C(O[[email protected]]2([H])[[email protected]@H](C)[[email protected]@H](O)[[email protected]@H](C)CC[[email protected]](O[[email protected]]4C)C[[email protected]@H](C4)OC)=O)C)O)([H])C=CC3)OC)C)O)O)C)[[email protected]@H](O)[[email protected]@H](C)CC[[email protected]](O[[email protected]]5C)C[[email protected]@H](C5)OC

Structure Classification
Source

Marine sponges

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Swinholide A
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