2. Cell Cycle/DNA Damage Cytoskeleton Apoptosis Autophagy
  3. Microtubule/Tubulin Apoptosis Autophagy
  4. TN-16

TN-16 is a Microtubule polymerization inhibitor. TN-16 induces G2/M cell cycle arrest, metaphase mitotic arrest and Apoptotic cell death in cells, and blocks late Autophagic flux by inhibiting autophagosome-lysosome fusion. TN-16 suppresses tumor growth in syngeneic mouse breast cancer models. TN-16 can be used in research related to neuroblastoma, cervical cancer, breast cancer and other tumors.

For research use only. We do not sell to patients.

TN-16

TN-16 Chemical Structure

CAS No. : 33016-12-5

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
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TN-16 Related Antibodies

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Description

TN-16 is a Microtubule polymerization inhibitor. TN-16 induces G2/M cell cycle arrest, metaphase mitotic arrest and Apoptotic cell death in cells, and blocks late Autophagic flux by inhibiting autophagosome-lysosome fusion. TN-16 suppresses tumor growth in syngeneic mouse breast cancer models. TN-16 can be used in research related to neuroblastoma, cervical cancer, breast cancer and other tumors[1][2][3].

In Vitro

TN-16 (1 μM; 72 h) potently reduces the viability of neuroblastoma SH-SY5Y cells, with an EC50 of 68 nM[1].
TN-16 exhibits cytotoxicity against cervical cancer HeLa cells[1].
TN-16 (200 nM-1 μM; 16 h) induces G2/M phase cell cycle arrest in neuroblastoma SH-SY5Y cells[1].
TN-16 (1 μM; 16 h) causes only an extremely slight change in the ratio of tubulin polymerization/depolymerization in neuroblastoma SH-SY5Y cells[1].
TN-16 (1 μM-10 μM; 61 min) inhibits the polymerization of purified tubulin in a dose-dependent manner, and a strong inhibitory effect is observed at a concentration of 10 μM[1].
TN-16 (1.25 µmol/L; 24-48 h) enhances apoptosis activation, which is manifested as increased cleavage levels of Caspase-3 and PARP in Atg7-knockdown HCT-116 (Bax+/-) cells[2].
Incubation with TN-16 (10 μM; up to 60 min) at 30°C for up to 60 min inhibits the assembly of porcine brain tubulin in vitro, as determined by turbidity measurement at 350 nm[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

TN-16 Related Antibodies

Cell Cytotoxicity Assay[1]

Cell Line: human neuroblastoma SH-SY5Y cells
Concentration: 1 μM
Incubation Time: 72 h
Result: Exhibited cytotoxic activity with an EC50 of 68 nM.
Reduced cell viability to 34.7% of control.

Cell Cycle Analysis[1]

Cell Line: human neuroblastoma SH-SY5Y cells
Concentration: 200 nM-1 μM
Incubation Time: 16 h
Result: Induced a distinct G2/M cell cycle arrest at both tested concentrations.

Western Blot Analysis[2]

Cell Line: Atg7-knockdown human HCT-116 (Bax+/-) colon cancer cells
Concentration: 1.25 µmol/L
Incubation Time: 24, 48 h
Result: Induced enhanced cleavage of caspase-3 and PARP in Atg7-knockdown cells relative to autophagy-proficient scrambled control cells.
In Vivo

TN-16 (1 mg/kg; i.p.; daily; for 11 consecutive days) significantly inhibits breast tumor growth in orthotopic mouse models, with its mechanism of action being the blockade of autophagic flux and induction of apoptosis[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Crl:CD1-Foxn1nu (5- to 6-week-old female; orthotopic syngeneic breast cancer model via transplantation of 1×106 4T1 mouse mammary carcinoma cells into the mammary fat pad)[2]
Dosage: 1 mg/kg
Administration: i.p.; daily; 11 days
Result: Reduced tumour volume to ~270 mm3 by day 11 post-treatment initiation versus ~520 mm3 in controls.
Lowered average harvested tumour weight significantly compared to controls.
Increased levels of cleaved PARP and cleaved caspase-3 in tumour tissue lysates.
Induced accumulation of p62 and increased LC3-II turnover, indicating blockade of autophagic flux and enhanced apoptosis.
Molecular Weight

306.36

Formula

C19H18N2O2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(NC(C/1=O)CC2=CC=CC=C2)C1=C(C)/NC3=CC=CC=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (163.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2641 mL 16.3207 mL 32.6413 mL
5 mM 0.6528 mL 3.2641 mL 6.5283 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation

Purity: 99.78%

SDS (597 KB)

COA (250 KB) HNMR (260 KB) LCMS (95 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.2641 mL 16.3207 mL 32.6413 mL 81.6033 mL
5 mM 0.6528 mL 3.2641 mL 6.5283 mL 16.3207 mL
10 mM 0.3264 mL 1.6321 mL 3.2641 mL 8.1603 mL
15 mM 0.2176 mL 1.0880 mL 2.1761 mL 5.4402 mL
20 mM 0.1632 mL 0.8160 mL 1.6321 mL 4.0802 mL
25 mM 0.1306 mL 0.6528 mL 1.3057 mL 3.2641 mL
30 mM 0.1088 mL 0.5440 mL 1.0880 mL 2.7201 mL
40 mM 0.0816 mL 0.4080 mL 0.8160 mL 2.0401 mL
50 mM 0.0653 mL 0.3264 mL 0.6528 mL 1.6321 mL
60 mM 0.0544 mL 0.2720 mL 0.5440 mL 1.3601 mL
80 mM 0.0408 mL 0.2040 mL 0.4080 mL 1.0200 mL
100 mM 0.0326 mL 0.1632 mL 0.3264 mL 0.8160 mL
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TN-16 Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

TN-1633016-12-5TN16TN 16Microtubule/TubulinApoptosisAutophagymicrotubule assemblyG2/M cell cycle arresthuman cervical cancer HeLa cellssyngeneic mouse breast cancer modelhuman neuroblastoma SH-SY5Y cellsporcine tubulinα-tubulin acetylationautophagic fluxapoptotic cell deathcolchicine-sensitive siteInhibitorinhibitorinhibit

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