1. Cell Cycle/DNA Damage
  2. DNA/RNA Synthesis
  3. Synucleozid hydrochloride

Synucleozid hydrochloride (Synonyms: NSC 377363 hydrochloride)

Cat. No.: HY-135902A Purity: 98.33%
Handling Instructions

Synucleozid hydrochloride (NSC 377363 hydrochloride) is a potent inhibitor of the SNCA mRNA that encodes α-synuclein protein (IC50=1.5 μM). Synucleozid selectively targets the α-synuclein mRNA 5′ UTR at the designed IRE site, decreases the amount of SNCA mRNA loaded into polysomes and thereby inhibits SNCA translation. Synucleozid has the potential for the investigation of Parkinson’s disease.

For research use only. We do not sell to patients.

Synucleozid hydrochloride Chemical Structure

Synucleozid hydrochloride Chemical Structure

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 308 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 308 In-stock
Estimated Time of Arrival: December 31
Solid
5 mg USD 280 In-stock
Estimated Time of Arrival: December 31
10 mg USD 440 In-stock
Estimated Time of Arrival: December 31
50 mg USD 1240 In-stock
Estimated Time of Arrival: December 31
100 mg USD 2240 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

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Description

Synucleozid hydrochloride (NSC 377363 hydrochloride) is a potent inhibitor of the SNCA mRNA that encodes α-synuclein protein (IC50=1.5 μM). Synucleozid selectively targets the α-synuclein mRNA 5′ UTR at the designed IRE site, decreases the amount of SNCA mRNA loaded into polysomes and thereby inhibits SNCA translation. Synucleozid has the potential for the investigation of Parkinson’s disease[1].

IC50 & Target

IC50: 1.5 μM (SNCA IRE RNA)[1]

In Vitro

Synucleozid (0.25-1 μM; 24 hours) abrogates cytotoxicity induced by α-synuclein preformed fibrils, which act as seeds and recruit endogenous α-synuclein to aggregate[1].
Synucleozid (0-1 μM; 24 hours) binds to the A bulge near the base of the IRE hairpin, reduced levels of α-synuclein in a dose-dependent manner with an IC50 of 500 nM, and inhibits α-synuclein protein expression in SH-SY5Y neuroblastoma cells[1].
Synucleozid (100 nM-100 μM; 24 hours) binds to 2-AP-labeled and native IRE RNA with similar affinities. It decreases 2-AP emission with an EC50 value of 2.7 μM, recovery of 2-AP emissions is observed as a function of unlabeled SNCA IRE RNA (RNA-0) concentration, affording a competitive Kd of 1.5 μM[1].
Synucleozid (0.25-1 μM; 24 hours) decreases α-synuclein and other proteins that have IREs in their mRNA’s UTR including APP, PrP, Ferritin and TfR as a dose-dependent mannner. All panels is completed in SH-SY5Y cells, except for PrP protein which is assessed in Neuro-2A cells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: SH-SY5Y neuroblastoma cells
Concentration: 0.25 μM, 0.5 μM, 1 μM
Incubation Time: 24 hours
Result: Decreased LDH release as a dose-dependent manner.

Western Blot Analysis[1]

Cell Line: SH-SY5Y neuroblastoma cells
Concentration: 0.25 μM, 0.5 μM, 1 μM
Incubation Time: 24 hours
Result: Decreased α-synuclein expression as a concentration-dependent manner.
Molecular Weight

404.90

Formula

C22H21ClN6

SMILES

N=C(C1=CC2=C(C=C1)C=C(C3=CC=C(NC4=CC=C(C(N)=N)C=C4)C=C3)N2)N.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (308.72 mM; Need ultrasonic)

H2O : 2 mg/mL (4.94 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4697 mL 12.3487 mL 24.6975 mL
5 mM 0.4939 mL 2.4697 mL 4.9395 mL
10 mM 0.2470 mL 1.2349 mL 2.4697 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (5.14 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (5.14 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 98.33%

References
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Product Name:
Synucleozid hydrochloride
Cat. No.:
HY-135902A
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