Uridine 5'-O-thiodiphosphate
Uridine 5'-O-thiodiphosphate (UDP-β-S) is a P2Y6 receptor agonist with an EC50 of 25 nM. Uridine 5'-O-thiodiphosphate resists degradation by extracellular nucleotidases and stimulates the accumulation of inositol phosphates. Uridine 5'-O-thiodiphosphate stimulates contractile responses in endothelium-denuded rat mesenteric arteries. As a mitogen, Uridine 5'-O-thiodiphosphate stimulates DNA synthesis, [3H] thymidine incorporation, protein synthesis, [3H]leucine incorporation, and increases the number of vascular smooth muscle cells. Uridine 5'-O-thiodiphosphate can be used in the research of cardiovascular diseases such as atherosclerosis.
For research use only. We do not sell to patients.
- CAS No.: 221010-51-1
- Formula: C9H14N2O11P2S
- Molecular Weight:420.23
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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P2Y6 Receptor 25 nM (EC50) |
Uridine 5'-O-thiodiphosphate is a potent and selective agonist of the human P2Y6 receptor expressed in 1321N1 cells, with an EC50 of 28 nM, and shows no activity against human P2Y1, P2Y2 or P2Y4 receptors[2].
Uridine 5'-O-thiodiphosphate (1-30 μM; 19 h) stimulates DNA synthesis in rat aortic vascular smooth muscle cells, with an Emax that is 73% higher than that of the control group and a pEC50 of 5.42[2].
Uridine 5'-O-thiodiphosphate (30 μM; 96 h) increases protein synthesis in rat aortic vascular smooth muscle cells by 25% compared with the control group[2].
Uridine 5'-O-thiodiphosphate (30 μM; 0-120 min) is resistant to degradation by nucleotideases in 1321N1 astrocytoma cells over a 120-minute incubation period[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:rat aorta vascular smooth muscle cells (VSMC)
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Concentration:1-30 μM
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Incubation Time:19 h
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Result:Induced a concentration-dependent increase in [3H]thymidine incorporation, with an Emax of 73% above control and a pEC50 of 5.42.
Stimulated [3H]thymidine incorporation by 36% above control at 1 μM, and showed an additive effect when combined with 1 μM UTPγS.
Stimulated [3H]thymidine incorporation to levels comparable to UDP at 30 μM.
Chemical Information
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CAS No. 221010-51-1
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Molecular Weight 420.23
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Formula C9H14N2O11P2S
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SMILES
O[C@H]1[C@@H](O)[C@H](N2C=CC(NC2=O)=O)O[C@@H]1COP(OP(O)(S)=O)(O)=O
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Synonyms
UDP-β-S
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Malmsjö, et al. Characterization of contractile P2 receptors in human coronary arteries by use of the stable pyrimidines uridine 5′-O-thiodiphosphate and uridine 5′-O-3-thiotriphosphate. Journal of Pharmacology and Experimental Therapeutics 293.3 (2000): 755-760. [Content Brief]
[2]. Hou M, et al. UDP acts as a growth factor for vascular smooth muscle cells by activation of P2Y(6) receptors. Am J Physiol Heart Circ Physiol. 2002;282(2):H784-H792. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Uridine 5'-O-thiodiphosphate Related Classifications
HY-137608 Related Classifications
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