Uridine 5'-O-thiodiphosphate  (Synonyms: UDP-β-S)

Uridine 5'-O-thiodiphosphate (UDP-β-S) is a P2Y₆ receptor agonist with an EC₅₀ of 25 nM. Uridine 5'-O-thiodiphosphate resists degradation by extracellular nucleotidases and stimulates the accumulation of inositol phosphates. Uridine 5'-O-thiodiphosphate stimulates contractile responses in endothelium-denuded rat mesenteric arteries. As a mitogen, Uridine 5'-O-thiodiphosphate stimulates DNA synthesis, [³H] thymidine incorporation, protein synthesis, [³H]leucine incorporation, and increases the number of vascular smooth muscle cells. Uridine 5'-O-thiodiphosphate can be used in the research of cardiovascular diseases such as atherosclerosis^[1][2].

Uridine 5'-O-thiodiphosphate is a potent and selective agonist of the human P2Y₆ receptor expressed in 1321N1 cells, with an EC₅₀ of 28 nM, and shows no activity against human P2Y₁, P2Y₂ or P2Y₄ receptors^[2].
Uridine 5'-O-thiodiphosphate (1-30 μM; 19 h) stimulates DNA synthesis in rat aortic vascular smooth muscle cells, with an E_max that is 73% higher than that of the control group and a pEC₅₀ of 5.42^[2].
Uridine 5'-O-thiodiphosphate (30 μM; 96 h) increases protein synthesis in rat aortic vascular smooth muscle cells by 25% compared with the control group^[2].
Uridine 5'-O-thiodiphosphate (30 μM; 0-120 min) is resistant to degradation by nucleotideases in 1321N1 astrocytoma cells over a 120-minute incubation period^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

420.23

C₉H₁₄N₂O₁₁P₂S

221010-51-1

O[C@H]1[C@@H](O)[C@H](N2C=CC(NC2=O)=O)O[C@@H]1COP(OP(O)(S)=O)(O)=O

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• [1]. Malmsjö, et al. Characterization of contractile P2 receptors in human coronary arteries by use of the stable pyrimidines uridine 5′-O-thiodiphosphate and uridine 5′-O-3-thiotriphosphate. Journal of Pharmacology and Experimental Therapeutics 293.3 (2000): 755-760.  [Content Brief]

  [2]. Hou M, et al. UDP acts as a growth factor for vascular smooth muscle cells by activation of P2Y(6) receptors. Am J Physiol Heart Circ Physiol. 2002;282(2):H784-H792.  [Content Brief]