Uridine 5'-O-thiodiphosphate trisodium
Uridine 5'-O-thiodiphosphate trisodium (UDP-β-S trisodium) is a P2Y6 receptor agonist with an EC50 of 25 nM. Uridine 5'-O-thiodiphosphate trisodium resists degradation by extracellular nucleotidases and stimulates the accumulation of inositol phosphates. Uridine 5'-O-thiodiphosphate trisodium stimulates contractile responses in endothelium-denuded rat mesenteric arteries. As a mitogen, Uridine 5'-O-thiodiphosphate trisodium stimulates DNA synthesis, [3H] thymidine incorporation, protein synthesis, [3H]leucine incorporation, and increases the number of vascular smooth muscle cells. Uridine 5'-O-thiodiphosphate trisodium can be used in the research of cardiovascular diseases such as atherosclerosis.
For research use only. We do not sell to patients.
- Formula: C9H11N2Na3O11P2S
- Molecular Weight:486.17
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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P2Y6 Receptor 25 nM (EC50) |
Uridine 5'-O-thiodiphosphate trisodium is a potent and selective agonist of the human P2Y6 receptor expressed in 1321N1 cells, with an EC50 of 28 nM, and shows no activity against human P2Y1, P2Y2 or P2Y4 receptors[2].
Uridine 5'-O-thiodiphosphate trisodium (1-30 μM; 19 h) stimulates DNA synthesis in rat aortic vascular smooth muscle cells, with an Emax that is 73% higher than that of the control group and a pEC50 of 5.42[2].
Uridine 5'-O-thiodiphosphate trisodium (30 μM; 96 h) increases protein synthesis in rat aortic vascular smooth muscle cells by 25% compared with the control group[2].
Uridine 5'-O-thiodiphosphate trisodium (30 μM; 0-120 min) is resistant to degradation by nucleotideases in 1321N1 astrocytoma cells over a 120-minute incubation period[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:rat aorta vascular smooth muscle cells (VSMC)
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Concentration:1-30 μM
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Incubation Time:19 h
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Result:Induced a concentration-dependent increase in [3H]thymidine incorporation, with an Emax of 73% above control and a pEC50 of 5.42.
Stimulated [3H]thymidine incorporation by 36% above control at 1 μM, and showed an additive effect when combined with 1 μM UTPγS.
Stimulated [3H]thymidine incorporation to levels comparable to UDP at 30 μM.
Chemical Information
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Molecular Weight 486.17
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Formula C9H11N2Na3O11P2S
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SMILES
O[C@H]1[C@@H](O)[C@H](N2C=CC(NC2=O)=O)O[C@@H]1COP(OP(O[Na])(S[Na])=O)(O[Na])=O
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Synonyms
UDP-β-S trisodium
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Malmsjö M, et al. The stable pyrimidines UDPbetaS and UTPgammaS discriminate between the P2 receptors that mediate vascular contraction and relaxation of the rat mesenteric artery. Br J Pharmacol. 2000;131(1):51-56. [Content Brief]
[2]. Hou M, et al. UDP acts as a growth factor for vascular smooth muscle cells by activation of P2Y(6) receptors. Am J Physiol Heart Circ Physiol. 2002;282(2):H784-H792. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Uridine 5'-O-thiodiphosphate trisodium Related Classifications
HY-137608A Related Classifications
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