2. Metabolic Enzyme/Protease
  3. Aldehyde Dehydrogenase (ALDH)
  4. Win 18446

Win 18446 

Cat. No.: HY-W011094 Purity: 98.57%
COA Handling Instructions

Win 18446 is an orally active testes-specific enzyme ALDH1a2 inhibitor, with an IC50 of 0.3 μM. Win 18446 reversibly inhibits spermatogenesis in many species and inhibits Retinoic acid (HY-14649) biosynthesis from Retinol (HY-B1342) within the testes.

For research use only. We do not sell to patients.

Win 18446 Chemical Structure

Win 18446 Chemical Structure

CAS No. : 1477-57-2

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Solution
10 mM * 1 mL in DMSO USD 121 In-stock
Estimated Time of Arrival: December 31
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ready for reconstitution
 USD 121 In-stock
Estimated Time of Arrival: December 31
Solid
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Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Win 18446

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Win 18446 is an orally active testes-specific enzyme ALDH1a2 inhibitor, with an IC50 of 0.3 μM. Win 18446 reversibly inhibits spermatogenesis in many species and inhibits Retinoic acid (HY-14649) biosynthesis from Retinol (HY-B1342) within the testes[1][2].

In Vitro

WIN 18446 (1 μM, 24 h) is able to suppress Stra8 expression in whole-testes cultures that contained both germ and somatic cells[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR[2]

Cell Line: Testes were collected from 2-dpp mice.
Concentration: 1 μM.
Incubation Time: 24 h.
Result: Results in a VAD-like phenotype in the testis.
Alone or in combination with ROL significantly reduced Stra8 expression.
In Vivo

Win 18446 (200 mg/kg, Orally for 4, 8, and 16 weeks) significantly reduces intratesticular concentrations of retinoic acid, severely impaired spermatogenesis, and caused infertility[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Six-month-old male New Zealand white rabbits[1].
Dosage: 200 mg/kg.
Administration: Orally for 4, 8, and 16 weeks.
Result: Resulted in marked suppression of testicular weight over time.
Dramatically suppressed spermatogenesis.
Significantly reduces intratesticular concentrations of retinoic acid and Stra8 expression and very severely suppresses spermatogenesis and fertility.
Molecular Weight

366.11

Appearance

Solid

Formula

C12H20Cl4N2O2
CAS No.
SMILES

O=C(NCCCCCCCCNC(C(Cl)Cl)=O)C(Cl)Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (273.14 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7314 mL 13.6571 mL 27.3142 mL
5 mM 0.5463 mL 2.7314 mL 5.4628 mL
10 mM 0.2731 mL 1.3657 mL 2.7314 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (5.68 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 98.57%

COA (241 KB) HNMR (253 KB) LCMS (261 KB)

Handling Instructions (2659 KB)
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

Win 184461477-57-2Win18446Win-18446Aldehyde Dehydrogenase (ALDH)Stra8TestestestisspermatogenesisInhibitorinhibitorinhibit

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