2. Metabolic Enzyme/Protease
  3. Aldehyde Dehydrogenase (ALDH)
  4. Win 18446

Win 18446 is an orally active testes-specific enzyme ALDH1a2 inhibitor, with an IC50 of 0.3 μM. Win 18446 reversibly inhibits spermatogenesis in many species and inhibits Retinoic acid (HY-14649) biosynthesis from Retinol (HY-B1342) within the testes.

For research use only. We do not sell to patients.

CAS No. : 1477-57-2

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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10 mM * 1 mL in DMSO In-stock
Solid
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100 mg In-stock
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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of Win 18446:

Win 18446 Related Antibodies

Top Publications Citing Use of Products

    Win 18446 purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2026 Jan 29;302(3):111211.  [Abstract]

    Win18446 (Win446, 1 μM). The ATRA content of each fraction is presented as a portion of total levels. Data presented as mean ± S.E. from 5 separate experiments. H–J, mutuDC1940 cells were treated with 9cRA and assayed with 10 μM RAL.

    Win 18446 purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2026 Jan 29;302(3):111211.  [Abstract]

    ROL abundance analysis was performed on mutuDC1940 cells treated with 9cRA, 9cRA + 1 μM Win 18446 (Win446), or 9cRA + 10 μM DEAB.

    Win 18446 purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2026 Jan 29;302(3):111211.  [Abstract]

    4OHRA abundance analysis was performed on mutuDC1940 cells treated with 9cRA, 9cRA + 1 μM Win 18446 (Win446), or 9cRA + 10 μM DEAB.

    Win 18446 purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2026 Jan 29;302(3):111211.  [Abstract]

    mutuDC1940 cells were treated for 20 h with or without 1 μM 9cRA, then subjected to the AldeRed retention assay in the presence or absence of 1 μM Win446 or 10 μM DEAB. The AldeRed retention assay was performed with 0.61 μg/ml AldeRed at either low (0.2 × 106 cells/ml) or high (1 × 106 cells/ml) cell density.

    Win 18446 purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2026 Jan 29;302(3):111211.  [Abstract]

    mutuDC1940 cells were treated as Win18446 (Win446, 1 μM), except Medium B was replaced with the assay buffer from the AldeRed assay kit (Merck, catalogue #SCR150) for the ATRA production assay.

    View All Aldehyde Dehydrogenase (ALDH) Isoform Specific Products:

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    ALDH1 ALDH2 ALDH3

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Win 18446 is an orally active testes-specific enzyme ALDH1a2 inhibitor, with an IC50 of 0.3 μM. Win 18446 reversibly inhibits spermatogenesis in many species and inhibits Retinoic acid (HY-14649) biosynthesis from Retinol (HY-B1342) within the testes[1][2].

    IC50 & Target

    ALDH1

     

    In Vitro

    WIN 18446 (1 μM, 24 h) is able to suppress Stra8 expression in whole-testes cultures that contained both germ and somatic cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Win 18446 Related Antibodies

    RT-PCR[2]

    Cell Line: Testes were collected from 2-dpp mice.
    Concentration: 1 μM.
    Incubation Time: 24 h.
    Result: Results in a VAD-like phenotype in the testis.
    Alone or in combination with ROL significantly reduced Stra8 expression.
    In Vivo

    Win 18446 (200 mg/kg, Orally for 4, 8, and 16 weeks) significantly reduces intratesticular concentrations of retinoic acid, severely impaired spermatogenesis, and caused infertility[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Six-month-old male New Zealand white rabbits[1].
    Dosage: 200 mg/kg.
    Administration: Orally for 4, 8, and 16 weeks.
    Result: Resulted in marked suppression of testicular weight over time.
    Dramatically suppressed spermatogenesis.
    Significantly reduces intratesticular concentrations of retinoic acid and Stra8 expression and very severely suppresses spermatogenesis and fertility.
    Molecular Weight

    366.11

    Formula

    C12H20Cl4N2O2
    CAS No.
    Appearance

    Solid

    Color

    White to light brown

    SMILES

    O=C(NCCCCCCCCNC(C(Cl)Cl)=O)C(Cl)Cl
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, stored under nitrogen

    *In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (273.14 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.7314 mL 13.6571 mL 27.3142 mL
    5 mM 0.5463 mL 2.7314 mL 5.4628 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Volume
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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

    V1

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    C2

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    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (5.68 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 98.94%

    SDS (393 KB)

    COA (241 KB) HNMR (255 KB) LCMS (92 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.7314 mL 13.6571 mL 27.3142 mL 68.2855 mL
    5 mM 0.5463 mL 2.7314 mL 5.4628 mL 13.6571 mL
    10 mM 0.2731 mL 1.3657 mL 2.7314 mL 6.8285 mL
    15 mM 0.1821 mL 0.9105 mL 1.8209 mL 4.5524 mL
    20 mM 0.1366 mL 0.6829 mL 1.3657 mL 3.4143 mL
    25 mM 0.1093 mL 0.5463 mL 1.0926 mL 2.7314 mL
    30 mM 0.0910 mL 0.4552 mL 0.9105 mL 2.2762 mL
    40 mM 0.0683 mL 0.3414 mL 0.6829 mL 1.7071 mL
    50 mM 0.0546 mL 0.2731 mL 0.5463 mL 1.3657 mL
    60 mM 0.0455 mL 0.2276 mL 0.4552 mL 1.1381 mL
    80 mM 0.0341 mL 0.1707 mL 0.3414 mL 0.8536 mL
    100 mM 0.0273 mL 0.1366 mL 0.2731 mL 0.6829 mL
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    Win 18446 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Win 184461477-57-2Win18446Win-18446Aldehyde Dehydrogenase (ALDH)Stra8TestestestisspermatogenesisInhibitorinhibitorinhibit

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