1. Metabolic Enzyme/Protease
  2. Aldehyde Dehydrogenase (ALDH)
  3. Win 18446

Win 18446 

Cat. No.: HY-W011094
Handling Instructions

Win 18446 is an orally active testes-specific enzyme ALDH1a2 inhibitor, with an IC50 of 0.3 μM. Win 18446 reversibly inhibits spermatogenesis in many species and inhibits Retinoic acid (HY-14649) biosynthesis from Retinol (HY-B1342) within the testes.

For research use only. We do not sell to patients.

Win 18446 Chemical Structure

Win 18446 Chemical Structure

CAS No. : 1477-57-2

Size Price Stock Quantity
10 mM * 1  mL in DMSO USD 110 In-stock
Estimated Time of Arrival: December 31
50 mg USD 100 In-stock
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

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Description

Win 18446 is an orally active testes-specific enzyme ALDH1a2 inhibitor, with an IC50 of 0.3 μM. Win 18446 reversibly inhibits spermatogenesis in many species and inhibits Retinoic acid (HY-14649) biosynthesis from Retinol (HY-B1342) within the testes[1][2].

In Vitro

WIN 18446 (1 μM, 24 h) is able to suppress Stra8 expression in whole-testes cultures that contained both germ and somatic cells[2].

RT-PCR[2]

Cell Line: Testes were collected from 2-dpp mice.
Concentration: 1 μM.
Incubation Time: 24 h.
Result: Results in a VAD-like phenotype in the testis.
Alone or in combination with ROL significantly reduced Stra8 expression.
In Vivo

Win 18446 (200 mg/kg, Orally for 4, 8, and 16 weeks) significantly reduces intratesticular concentrations of retinoic acid, severely impaired spermatogenesis, and caused infertility[1].

Animal Model: Six-month-old male New Zealand white rabbits[1].
Dosage: 200 mg/kg.
Administration: Orally for 4, 8, and 16 weeks.
Result: Resulted in marked suppression of testicular weight over time.
Dramatically suppressed spermatogenesis.
Significantly reduces intratesticular concentrations of retinoic acid and Stra8 expression and very severely suppresses spermatogenesis and fertility.
Molecular Weight

366.11

Formula

C₁₂H₂₀Cl₄N₂O₂

CAS No.

1477-57-2

SMILES

O=C(NCCCCCCCCNC(C(Cl)Cl)=O)C(Cl)Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility
In Vitro: 

DMSO : 10.87 mg/mL (29.69 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7314 mL 13.6571 mL 27.3142 mL
5 mM 0.5463 mL 2.7314 mL 5.4628 mL
10 mM 0.2731 mL 1.3657 mL 2.7314 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 1.09 mg/mL (2.98 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Keywords:

Win 18446Win18446Win-18446Aldehyde Dehydrogenase (ALDH)Stra8TestestestisspermatogenesisInhibitorinhibitorinhibit

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Win 18446
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HY-W011094
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