Adavivint (Synonyms: SM04690; Lorecivivint)

Name: Adavivint
Brand: MedChemExpress
SKU: HY-109049
Rating: 5.0 (10 reviews)

Cat. No.: HY-109049 Purity: 98.85%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Adavivint (SM04690; Lorecivivint) is a potent and selective inhibitor of canonical Wnt signaling, with an EC₅₀ of 19.5 nM via a high-throughput TCF/LEF-reporter assay in SW480 colon cancer cells.

For research use only. We do not sell to patients.

CAS No. : 1467093-03-3

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 10 publication(s) in Google Scholar

Other Forms of Adavivint:

Adavivint (Standard) Get quote

10 Publications Citing Use of MCE Adavivint

Cell Proliferation/Viability Assay

RT-PCR

: Adavivint purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2024 Aug 1.; Using the CCK8 assay, the cytotoxic effects of different concentrations of SM04690 (10 nM, 30 nM, 100 nM, 300 nM, 1 μM, 3 μM) on chondrocytes were examined.

: Adavivint purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2024 Aug 1.; After pretreatment with IL-1β, the gene expression of Aggrecan, COL2A1, ADAMTS5, and MMP 13 in chondrocytes was evaluated using various concentrations of SM04690 (30 nM, 0.1 μM, 0.3 μM).

: Adavivint purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2024 Aug 1.; After pretreatment with IL-1β and subsequent addition of SM04690 (30 nM) or KGN (40 μM), the gene expression of Aggrecan, COL2A1, ADAMTS5, and MMP 13 was evaluated.

: Adavivint purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2024 Aug 1.; After pretreatment with IL-1β and subsequent addition of SM04690 (30 nM) or KGN (40 μM), protein expression of Aggrecan, COL2A1, ADAMTS5, and MMP 13 was measured following the aforementioned treatments.

: Adavivint purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2024 Aug 1.; After pretreatment with IL-1β and subsequent addition of SM04690 (30 nM) or KGN (40 μM), immunofluorescence combined with DAPI staining was used to detect the expression of Collagen II.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

EC50: 19.5 nM (Wnt, SW480 cell)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
HT-22	IC₅₀	2.1 μM Compound: 23	Cytotoxicity against mouse HT-22 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay Cytotoxicity against mouse HT-22 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay	[PMID: 36876904]

In Vitro

Adavivint (SM04690) is a potent and selective inhibitor of Wnt signaling, with an EC₅₀ of 19.5 nM via a high-throughput TCF/LEF-reporter assay in SW480 colon cancer cells, and shows no effect on SV40 luciferase reporter. Adavivint enhances aggregation of human mesenchymal stem cells (hMSCs) with an EC₅₀ of 10 nM. Adavivint (30 nM) protects chondrocytes from catabolic breakdown in vitro^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Adavivint (0.3 μg) enhances cartilage repair and protection in the rat acute cruciate ligament tear and partial medial meniscectomy osteoarthritis (ACLT + pMMx OA) model^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

505.55

Formula

C₂₉H₂₄FN₇O

CAS No.

1467093-03-3

Appearance

Solid

Color

Light yellow to gray

SMILES

CC(C)CC(NC1=CC(C2=CC3=C(NN=C3C(N4)=NC5=C4C(C6=CC=CC(F)=C6)=CN=C5)C=C2)=CN=C1)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (49.45 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.9780 mL	9.8902 mL	19.7804 mL
5 mM	0.3956 mL	1.9780 mL	3.9561 mL
10 mM	0.1978 mL	0.9890 mL	1.9780 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (4.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.85%

Select Batch:

Data Sheet (279 KB) SDS (396 KB)

COA (256 KB) HNMR (143 KB) RP-HPLC (251 KB) MS (84 KB)

Handling Instructions (2659 KB)

References

[1]. Deshmukh V, et al. A small-molecule inhibitor of the Wnt pathway (SM04690) as a potential disease modifying agent for the treatment of osteoarthritis of the knee. Osteoarthritis Cartilage. 2018 Jan;26(1):18-27. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.9780 mL	9.8902 mL	19.7804 mL	49.4511 mL
	5 mM	0.3956 mL	1.9780 mL	3.9561 mL	9.8902 mL
	10 mM	0.1978 mL	0.9890 mL	1.9780 mL	4.9451 mL
	15 mM	0.1319 mL	0.6593 mL	1.3187 mL	3.2967 mL
	20 mM	0.0989 mL	0.4945 mL	0.9890 mL	2.4726 mL
	25 mM	0.0791 mL	0.3956 mL	0.7912 mL	1.9780 mL
	30 mM	0.0659 mL	0.3297 mL	0.6593 mL	1.6484 mL
	40 mM	0.0495 mL	0.2473 mL	0.4945 mL	1.2363 mL

Adavivint Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Adavivint1467093-03-3SM04690 LorecivivintSM 04690SM-04690WntInhibitorinhibitorinhibit

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Adavivint (Synonyms: SM04690; Lorecivivint)

Customer Review

Other Forms of Adavivint:

Adavivint Related Antibodies

10 Publications Citing Use of MCE Adavivint

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Wnt Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Adavivint Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Adavivint Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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