Amlodipine besylate (Synonyms: Amlodipine benzenesulfonate)

Name: Amlodipine besylate
Brand: MedChemExpress
SKU: HY-B0317B
Rating: 5.0 (14 reviews)

Cat. No.: HY-B0317B Purity: 99.72%: Data Sheet
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Amlodipine besylate (Amlodipine benzenesulfonate), an antianginal agent and an orally active dihydropyridine calcium channel blocker, works by blocking the voltage-dependent L-type calcium channels, thereby inhibiting the initial influx of calcium. Amlodipine besylate can be used for the research of high blood pressure and cancer.

For research use only. We do not sell to patients.

CAS No. : 111470-99-6

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	Get quote
50 g	Get quote

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Customer Review

Based on 14 publication(s) in Google Scholar

Other Forms of Amlodipine besylate:

14 Publications Citing Use of MCE Amlodipine besylate

Cell Imaging/Staining

Cell Proliferation/Viability Assay

Flow Cytometry

In Vivo Efficacy Study

Histological Imaging/Staining

: Amlodipine besylate purchased from MedChemExpress. Usage Cited in: Food Chem Toxicol. 2025 Feb 10:115317. [Abstract]; A CCK-8 assay was used to detect the effect of AMs on the proliferation of Ishikawa cells. The results showed that the half-maximal inhibitory concentration (IC₅₀) of Amlodipine (AM) against Ishikawa cells (at 72 h) was 12.36 μM.

: Amlodipine besylate purchased from MedChemExpress. Usage Cited in: Food Chem Toxicol. 2025 Feb 10:115317. [Abstract]; Cell apoptosis rate was detected by flow cytometry using Annexin-V/PI staining. The results showed that the apoptotic rate of the Amlodipine (AM) (15 μM; 12 h)-treated Ishikawa cells was 16.8%, which was 2.16-fold higher than that in cells treated with DMSO.

: Amlodipine besylate purchased from MedChemExpress. Usage Cited in: Food Chem Toxicol. 2025 Feb 10:115317. [Abstract]; EdU staining showing the effect of Amlodipine (AM) (15 μM; 48 h) on Ishikawa cells. The EdU staining assay revealed that the EdU-positive rate of the AM-treated Ishikawa cells was significantly reduced compared with that of the control group.

: Amlodipine besylate purchased from MedChemExpress. Usage Cited in: Food Chem Toxicol. 2025 Feb 10:115317. [Abstract]; Amlodipine (AM) (15 or 20 mg/kg; i.p.; every other day for two weeks) inhibited tumor growth in a dose-dependent manner in BALB/c nude mice bearing subcutaneous xenografts established from Ishikawa cells.

: Amlodipine besylate purchased from MedChemExpress. Usage Cited in: Food Chem Toxicol. 2025 Feb 10:115317. [Abstract]; H&E and PCNA staining of sections from mice treated with Amlodipine (AM) (15 or 20 mg/kg; i.p.; every other day for two weeks).

: Amlodipine besylate purchased from MedChemExpress. Usage Cited in: Exp Mol Med. 2021 Apr;53(4):572-590. [Abstract]; Rat GMCs pretreated with the calcium channel blocker Amlodipine (10μM), inhibitors of PKC (Go6983; 10μM), c-Raf (AZ628, 10μM) and MEK1/2 (U0126, 10μM) for 30min were stimulated with sublytic C5b-9 for 4 0min or 2h. The intracellular calcium was determined by Fluo-4 AM. The results showed that Amlodipine effectively blocked the rise in intracellular calcium levels in rat GMCs induced by 40 min of sublytic C5b-9 stimulation.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

L-type calcium channel

Cellular Effect

Cell Line	Type	Value	Description	References
HepG2	EC₅₀	31.6 μM Compound: Amlodipine Besylate	Activation of human PXR expressed in human HepG2 (DPX-2) cells after 24 hrs by luciferase reporter gene based luminescent analysis Activation of human PXR expressed in human HepG2 (DPX-2) cells after 24 hrs by luciferase reporter gene based luminescent analysis	[PMID: 20966043]
HepG2	EC₅₀	31.6 μM Compound: Amlodipine Besylate	Activation of human PXR expressed in human HepG2 (DPX-2) cells assessed as induction of CYP3A4 after 24 hrs by luminescent analysis Activation of human PXR expressed in human HepG2 (DPX-2) cells assessed as induction of CYP3A4 after 24 hrs by luminescent analysis	[PMID: 20966043]

In Vitro

Amlodipine besylate (20-40 μM; 48 h) reduces BrdU incorporation to 68.6% and 26.3% at concentrations of 20 and 30 μM in A431 cells, respectively^[3].
Amlodipine besylate (30 μM; pretreated for 1 h) significantly attenuates the uridine 5′-triphosphate (UTP)-induced increases of [Ca²⁺]_i in A431 cells^[3].
Amlodipine besylate (30 μM) inhibits the store-operated Ca²⁺influx evoked by Thapsigargin in Fluo-3-loaded cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Amlodipine besylate (5 mg/kg/day; s.c. for 2 weeks) significantly decreases systolic blood pressure (SBP) in VSMC ATP2B1 KO mice^[4].
Amlodipine besylate (10 mg/kg; i.p. once daily for 20 days) causes a significant retardation of tumor growth and prolongs the survival of A431 tumor-bearing mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	ATP2B1^loxP/loxP mice^[4]
Dosage:	5 mg/kg/day
Administration:	Subcutaneously implanted osmotic pump for 2 weeks
Result:	Significantly decreased the blood pressure.

Molecular Weight

567.05

Formula

C₂₆H₃₁ClN₂O₈S

CAS No.

111470-99-6

Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=C(COCCN)NC(C)=C(C(OC)=O)C1C2=CC=CC=C2Cl)OCC.O=S(C3=CC=CC=C3)(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : ≥ 45 mg/mL (79.36 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 1 mg/mL (1.76 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7635 mL	8.8176 mL	17.6351 mL
5 mM	0.3527 mL	1.7635 mL	3.5270 mL
10 mM	0.1764 mL	0.8818 mL	1.7635 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.41 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.41 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (4.41 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 2 mg/mL (3.53 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.72%

Select Batch:

Data Sheet (282 KB) SDS (393 KB)

COA (258 KB) HNMR (296 KB) LCMS (331 KB)

Handling Instructions (2659 KB)

References

[1]. Yoshida J, et, al. Antitumor effects of amlodipine, a Ca2+ channel blocker, on human epidermoid carcinoma A431 cells in vitro and in vivo. Eur J Pharmacol. 2004 May 25;492(2-3):103-12. [Content Brief]

[2]. Okuyama Y, et, al. The effects of anti-hypertensive drugs and the mechanism of hypertension in vascular smooth muscle cell-specific ATP2B1 knockout mice. Hypertens Res. 2018 Feb;41(2):80-87. [Content Brief]

[3]. Kishen G. Bulsara, et al. Amlodipine.

[4]. Haria M, et al. Amlodipine. A reappraisal of its pharmacological properties and therapeutic use in cardiovascular disease [published correction appears in Drugs 1995 Nov;50(5):896]. Drugs. 1995;50(3):560-586. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	1.7635 mL	8.8176 mL	17.6351 mL	44.0878 mL
DMSO	5 mM	0.3527 mL	1.7635 mL	3.5270 mL	8.8176 mL
	10 mM	0.1764 mL	0.8818 mL	1.7635 mL	4.4088 mL
	15 mM	0.1176 mL	0.5878 mL	1.1757 mL	2.9392 mL
	20 mM	0.0882 mL	0.4409 mL	0.8818 mL	2.2044 mL
	25 mM	0.0705 mL	0.3527 mL	0.7054 mL	1.7635 mL
	30 mM	0.0588 mL	0.2939 mL	0.5878 mL	1.4696 mL
	40 mM	0.0441 mL	0.2204 mL	0.4409 mL	1.1022 mL
	50 mM	0.0353 mL	0.1764 mL	0.3527 mL	0.8818 mL
	60 mM	0.0294 mL	0.1470 mL	0.2939 mL	0.7348 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Amlodipine besylate Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Amlodipine besylate111470-99-6AmlodipineCalcium ChannelCa2+ channelsCa channelsoraldihydropyridineantianginalhypertensionInhibitorinhibitorinhibit

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Amlodipine besylate (Synonyms: Amlodipine benzenesulfonate)

Customer Review

Other Forms of Amlodipine besylate:

Amlodipine besylate Related Antibodies

14 Publications Citing Use of MCE Amlodipine besylate

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Calcium Channel Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Amlodipine besylate Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Amlodipine besylate Related Classifications

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