1. Dye Reagents
  2. CFSE

CFSE (Synonyms: 5(6)-Carboxyfluorescein diacetate succinimidyl ester; CFDA-SE; 5(6)-CFDA N-succinmidyl ester)

Cat. No.: HY-D0938 Purity: >97.0%
Handling Instructions

CFSE is a fluorescent dye which can track the cell division.

For research use only. We do not sell to patients.

CFSE Chemical Structure

CFSE Chemical Structure

CAS No. : 150347-59-4

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5 mg USD 70 In-stock
Estimated Time of Arrival: December 31
10 mg USD 100 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 6 publication(s) in Google Scholar

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CFSE is a fluorescent dye which can track the cell division.

In Vitro

CFSE is a fluorescent dye which can track the cell division[1]. To examine the ability of immune cells to migrate into live slices, a single-cell suspension of autologous splenocytes or PBMC is isolated, labeling them with CFSE, and adding them to the top of the slices on day 2 of culture. After 6 additional days of culture, the CFSE-labeling immune cells (green) are found to have migrated throughout the slices. No positive cells are found in the slices without the addition of CFSE-labeling immune cells[2].

Molecular Weight









Room temperature in continental US; may vary elsewhere.


-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 62.5 mg/mL (112.12 mM; Need ultrasonic)

Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7939 mL 8.9693 mL 17.9385 mL
5 mM 0.3588 mL 1.7939 mL 3.5877 mL
10 mM 0.1794 mL 0.8969 mL 1.7939 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (3.73 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (3.73 mM); Clear solution

*All of the co-solvents are provided by MCE.
Cell Assay

Single-cell suspensions of splenocytes (or PBMC) are stained with 1 µM CFSE in PBS for 9 min at 37°C, combined with 20 mL of 10% FBS RPMI-1640 medium at RT for 2 min, centrifuged, washed, and counted. 2×106 CFSE-labeling splenocytes are added to the top of the slices in 24-well membrane culture insert[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.


Purity: >97.0%

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The dilution calculator equation

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This equation is commonly abbreviated as: C1V1 = C2V2

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× = ×
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CFSE5(6)-Carboxyfluorescein diacetate succinimidyl esterCFDA-SE5(6)-CFDA N-succinmidyl esterOthersInhibitorinhibitorinhibit

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