1. Fluorescent Dye
  2. Cell staining analysis
  3. Cytoplasm Dyes
    Cell Cycle Assay
    Exosome Track
    Cell Viability
    Cytotoxicity Assay
    Endocytosis And Pinocytosis
  4. CFDA-SE

CFDA-SE  (Synonyms: 5(6)-Carboxyfluorescein diacetate succinimidyl ester; 5(6)-CFDA N-succinmidyl ester)

Cat. No.: HY-D0938 Purity: 99.01%
COA Handling Instructions

CFDA-SE (5(6)-Carboxyfluorescein diacetate succinimidyl ester) is an intracellular fluorescent dye which can track proliferating cells. Covalently bound CFDA-SE is divided equally between daughter cells, allowing discrimination of successive rounds of cell division.

For research use only. We do not sell to patients.

CFDA-SE Chemical Structure

CFDA-SE Chemical Structure

CAS No. : 150347-59-4

Size Price Stock Quantity
Free Sample (0.1 - 0.5 mg)   Apply Now  
5 mg USD 70 In-stock
Estimated Time of Arrival: December 31
10 mg USD 100 In-stock
Estimated Time of Arrival: December 31
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

Customer Review

Based on 39 publication(s) in Google Scholar

Top Publications Citing Use of Products

33 Publications Citing Use of MCE CFDA-SE

IF
Proliferation Assay

    CFDA-SE purchased from MCE. Usage Cited in: Proc Natl Acad Sci U S A. 2022 Aug 9;119(32):e2201899119.  [Abstract]

    Flow cytometry analysis of CD4+ T cell number (B), proliferation rate (C), and representative images of CFSE-based proliferation assay (D) of CD4+ T cells cocultured with AT2 cells from healthy, LLC-bearing GPX3fl/fl, or GPX3cKO mice respectively.

    CFDA-SE purchased from MCE. Usage Cited in: Theranostics. 2021 Feb 20;11(9):4251-4261.  [Abstract]

    Mouse MC38 cells are washed with PBS and labeled with 1 μM CFDA-SE in PBS for 30 minutes at room temperature.

    CFDA-SE purchased from MCE. Usage Cited in: Cancer Lett. 2022 May 17;541:215746.  [Abstract]

    PBMCs are labeled with carboxyfluorescein diacetate succinimidyl ester (CFSE) (2.5 μM). The proliferation of T cells is assessed by CFSE intensity using flow cytometry.

    CFDA-SE purchased from MCE. Usage Cited in: OncoImmunology. 2022 Feb 1;11(1):2032918.  [Abstract]

    Bone marrow cells are incubated with 5 μΜ CFSE (5, 6- carboxyfluorescein diacetate) for 10 min at 37°C.

    CFDA-SE purchased from MCE. Usage Cited in: J Cell Mol Med. 2021 Jul;25(14):7052-7065.  [Abstract]

    THP-1 cells are stained with 1 μM carbox fluorescenceindiacetate succinimidyl ester (CFSE) in 0.1% BSA for 10 minutes at 37°C and ended with PBS for 10 minutes.

    CFDA-SE purchased from MCE. Usage Cited in: Mol Nutr Food Res. 2022 Jan;66(2):e2100619.  [Abstract]

    CFDA-SE (10 μM) is added to bacterial suspension and then incubated at 37°C in the dark for 20 min. The four strains are labeled with fluorescent probe CFDA-SE and were gavaged to baby rats to evaluate their colonization ability. Compared to the control group, the CFDA-SE fluorescence is present in rats gavaged with the four strains.
    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    CFDA-SE (5(6)-Carboxyfluorescein diacetate succinimidyl ester) is an intracellular fluorescent dye which can track proliferating cells. Covalently bound CFDA-SE is divided equally between daughter cells, allowing discrimination of successive rounds of cell division[1][2].

    In Vitro

    CFDA-SE is a fluorescent dye which can track the cell division[1]. To examine the ability of immune cells to migrate into live slices, a single-cell suspension of autologous splenocytes or PBMC is isolated, labeling them with CFDA-SE, and adding them to the top of the slices on day 2 of culture. After 6 additional days of culture, the CFDA-SE-labeling immune cells (green) are found to have migrated throughout the slices. No positive cells are found in the slices without the addition of CFDA-SE-labeling immune cells[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    557.46

    Formula

    C58H38N2O22

    CAS No.
    SMILES

    CC(OC1=CC=C(C2(C(C=CC(C(ON3C(CCC3=O)=O)=O)=C4)=C4C(O2)=O)C(C=CC(OC(C)=O)=C5)=C5O6)C6=C1)=O.CC(OC7=CC=C(C8(C(C=C(C(ON9C(CCC9=O)=O)=O)C=C%10)=C%10C(O8)=O)C(C=CC(OC(C)=O)=C%11)=C%11O%12)C%12=C7)=O

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    -20°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (89.69 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.7939 mL 8.9693 mL 17.9385 mL
    5 mM 0.3588 mL 1.7939 mL 3.5877 mL
    10 mM 0.1794 mL 0.8969 mL 1.7939 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.08 mg/mL (3.73 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.08 mg/mL (3.73 mM); Clear solution

    *All of the co-solvents are available by MCE.
    Purity & Documentation

    Purity: 99.01%

    Dyeing Example
    References
    Cell Assay
    [2]

    Single-cell suspensions of splenocytes (or PBMC) are stained with 1 µM CFSE in PBS for 9 min at 37°C, combined with 20 mL of 10% FBS RPMI-1640 medium at RT for 2 min, centrifuged, washed, and counted. 2×106 CFSE-labeling splenocytes are added to the top of the slices in 24-well membrane culture insert[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

     

    Salutation

    Applicant Name *

     

    Email address *

    Phone number *

     

    Organization name *

    Department *

     

    Requested quantity *

    Country or Region *

         

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    CFDA-SE
    Cat. No.:
    HY-D0938
    Quantity:
    MCE Japan Authorized Agent: