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  2. CFSE

CFSE (Synonyms: 5(6)-Carboxyfluorescein diacetate succinimidyl ester; CFDA-SE; 5(6)-CFDA N-succinmidyl ester)

Cat. No.: HY-D0938 Purity: 98.26%
Handling Instructions

CFSE (5(6)-Carboxyfluorescein diacetate succinimidyl ester) is an intracellular fluorescent dye which can track proliferating cells. Covalently bound CFSE is divided equally between daughter cells, allowing discrimination of successive rounds of cell division.

For research use only. We do not sell to patients.

CFSE Chemical Structure

CFSE Chemical Structure

CAS No. : 150347-59-4

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5 mg USD 70 In-stock
Estimated Time of Arrival: December 31
10 mg USD 100 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 8 publication(s) in Google Scholar

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Description

CFSE (5(6)-Carboxyfluorescein diacetate succinimidyl ester) is an intracellular fluorescent dye which can track proliferating cells. Covalently bound CFSE is divided equally between daughter cells, allowing discrimination of successive rounds of cell division[1][2].

In Vitro

CFSE is a fluorescent dye which can track the cell division[1]. To examine the ability of immune cells to migrate into live slices, a single-cell suspension of autologous splenocytes or PBMC is isolated, labeling them with CFSE, and adding them to the top of the slices on day 2 of culture. After 6 additional days of culture, the CFSE-labeling immune cells (green) are found to have migrated throughout the slices. No positive cells are found in the slices without the addition of CFSE-labeling immune cells[2].

Molecular Weight

557.46

Formula

C₂₉H₁₉NO₁₁

CAS No.

150347-59-4

SMILES

CC(OC1=CC=C(C2(C(C=CC(C(ON3C(CCC3=O)=O)=O)=C4)=C4C(O2)=O)C(C=CC(OC(C)=O)=C5)=C5O6)C6=C1)=O.CC(OC7=CC=C(C8(C(C=C(C(ON9C(CCC9=O)=O)=O)C=C%10)=C%10C(O8)=O)C(C=CC(OC(C)=O)=C%11)=C%11O%12)C%12=C7)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility
In Vitro: 

DMSO : 62.5 mg/mL (112.12 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7939 mL 8.9693 mL 17.9385 mL
5 mM 0.3588 mL 1.7939 mL 3.5877 mL
10 mM 0.1794 mL 0.8969 mL 1.7939 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (3.73 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (3.73 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Cell Assay
[2]

Single-cell suspensions of splenocytes (or PBMC) are stained with 1 µM CFSE in PBS for 9 min at 37°C, combined with 20 mL of 10% FBS RPMI-1640 medium at RT for 2 min, centrifuged, washed, and counted. 2×106 CFSE-labeling splenocytes are added to the top of the slices in 24-well membrane culture insert[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: 98.26%

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Keywords:

CFSE5(6)-Carboxyfluorescein diacetate succinimidyl esterCFDA-SE5(6)-CFDA N-succinmidyl esterOthersInhibitorinhibitorinhibit

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CFSE
Cat. No.:
HY-D0938
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