Quantifying CFSE Label Decay in Flow Cytometry Data

  • Appl Math Lett. 2013 May 1;26(5):571-577. doi: 10.1016/j.aml.2012.12.010.
H T Banks  1 ,  A Choi ,  T Huffman ,  J Nardini ,  L Poag ,  W C Thompson
Affiliations
  • 1. Center for Research in Scientific Computation, Center for Quantitative Sciences in Biomedicine, North Carolina State University, Raleigh, NC.
PMID: 23526173 DOI: 10.1016/j.aml.2012.12.010
Abstract

We developed a series of models for the label decay in cell proliferation assays when the intracellular dye carboxyfluorescein succinimidyl ester (CFSE) is used as a staining agent. Data collected from two healthy patients were used to validate the models and to compare the models with the Akiake Information Criteria. The distinguishing features of multiple decay rates in the data are readily characterized and explained via time dependent decay models such as the logistic and Gompertz models.

Keywords
Akiake Information; Gompertz growth; Michaelis-Menten kinetics; carboxyfluorescein succinimidyl ester (CFSE); exponential decay; inverse problems; logistic growth; ordinary differential equation models.
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