Indisulam (Synonyms: E 7070)

Name: Indisulam
Brand: MedChemExpress
SKU: HY-13650
Rating: 5.0 (15 reviews)

Cat. No.: HY-13650 Purity: 99.80%: Data Sheet
COA

SDS
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Indisulam (E 7070) is a carbonic anhydrase inhibitor with anticancer activity. Indisulam (E 7070) is a sulfonamide agent that targets the G1 phase of the cell cycle. Indisulam (E 7070) causes a blockade in the G1/S transition through inhibition of the activation of both CDK2 and cyclin E. Indisulam (E 7070) targets splicing by inducing RBM39 degradation via recruitment to DCAF15.

For research use only. We do not sell to patients.

CAS No. : 165668-41-7

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	Get quote
1 g	Get quote

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Customer Review

Based on 15 publication(s) in Google Scholar

Other Forms of Indisulam:

Indisulam (Standard) Get quote

15 Publications Citing Use of MCE Indisulam

Cell Imaging/Staining

Apoptosis Analysis

In Vivo Efficacy Study

IHC

: Indisulam purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2024 Jul 24;43(1):205. [Abstract]; Fluorescence microscopy images of the T-ALL cell lines J.gamma1 and Jurkat after 48 h of treatment with 1 μM and 5 μM Indisulam, respectively.

: Indisulam purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2024 Jul 24;43(1):205. [Abstract]; Flow cytometry analysis was performed to assess apoptosis in T-ALL cells after 24 h of treatment with DMSO or various concentrations of Indisulam (1 μM and 5 μM) utilizing Annexin V and PI staining. The percentage of apoptotic cells was subjected to statistical evaluation.

: Indisulam purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2024 Jul 24;43(1):205. [Abstract]; Western blot analysis was performed to validate the protein expression changes occurring in the J.gamma1 and Jurkat cell lines after treatment with Indisulam (1 μM and 5 μM) for 24 and 48 h, respectively.

: Indisulam purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2024 Jul 24;43(1):205. [Abstract]; Compared with the control group, the Indisulam (12.5 mg/kg, i.p.)-treated group displayed a significant decrease in fluorescence intensity at four distinct time points posttreatment, demonstrating a substantial suppression of J.gamma1 cell proliferation in vivo.

: Indisulam purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2024 Jul 24;43(1):205. [Abstract]; Immunohistochemical analyses of the Indisulam (12.5 mg/kg, i.p.)-treated group the mouse bone, liver, and spleen tissues revealed a decreased expression of Ki67 and RBM39.

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CA CA I CA Ⅱ CA IX CA XII CA VII CA VI CA VA CA VB CA XIII CA XIV

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Carbonic anhydrase^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	IC₅₀	0.21 μM Compound: 3	Effective concentration to inhibit cell proliferation by 50% relative to untreated control cell after 72 hr of continuous exposure in HCT116-C9 cell line Effective concentration to inhibit cell proliferation by 50% relative to untreated control cell after 72 hr of continuous exposure in HCT116-C9 cell line	[PMID: 12383017]
HCT-116	IC₅₀	29 μM Compound: 3	Effective concentration to inhibit cell proliferation by 50% relative to untreated control cell after 72 hr of continuous exposure in HCT116-C9 -C1cell line Effective concentration to inhibit cell proliferation by 50% relative to untreated control cell after 72 hr of continuous exposure in HCT116-C9 -C1cell line	[PMID: 12383017]
KB	IC₅₀	4.4 μg/mL Compound: 25(E7070)	Antiproliferative activity against KB human nasopharynx carcinoma in vitro Antiproliferative activity against KB human nasopharynx carcinoma in vitro	[PMID: 10508428]
MC-38	IC₅₀	0.1 μg/mL Compound: 25(E7070)	In vitro antiproliferative activity against colon 38 murine adenocarcinoma In vitro antiproliferative activity against colon 38 murine adenocarcinoma	[PMID: 10508428]
MCF7	IC₅₀	36.3 μM Compound: E7070	Antiproliferative activity against human MCF7 cells assessed as reduction in cell proliferation incubated for 48 hrs by Sulfo-Rhodamine-B stain (SRB) assay Antiproliferative activity against human MCF7 cells assessed as reduction in cell proliferation incubated for 48 hrs by Sulfo-Rhodamine-B stain (SRB) assay	[PMID: 32707525]
P388	IC₅₀	0.45 μg/mL Compound: 25(E7070)	Antiproliferative activity against P388 murine leukemia in vitro Antiproliferative activity against P388 murine leukemia in vitro	[PMID: 10508428]
P388	IC₅₀	0.59 μM Compound: 3	Effective concentration to inhibit cell proliferation by 50% relative to untreated control cell after 72 hr of continuous exposure in P388/4.0 r-M cell line Effective concentration to inhibit cell proliferation by 50% relative to untreated control cell after 72 hr of continuous exposure in P388/4.0 r-M cell line	[PMID: 12383017]
P388	IC₅₀	0.61 μM Compound: 3	Effective concentration to inhibit cell proliferation by 50% relative to untreated control cell after 72 hr of continuous exposure in P388 cell line Effective concentration to inhibit cell proliferation by 50% relative to untreated control cell after 72 hr of continuous exposure in P388 cell line	[PMID: 12383017]

In Vitro

Indisulam (E7070, 0-50 μg/mL, 12-48 h) causes cell cycle perturbation in the G1 phase in a time- and dose-dependent manner, potentially leading to cell death^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cycle Analysis^[1]

Cell Line:	P388 murine leukaemia cells.
Concentration:	0-50 μg/mL.
Incubation Time:	12-48 h.
Result:	Accumulated P388 cells in the G1 phase.

In Vivo

Indisulam (E7070, 12.5, 25, 50 (100) mg/kg) shows an antitumour spectrum in human cancer models^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female BALB/c nu/nu mice aged 7 weeks (HCT116 colon, LX-1 lung, SW620 colon, HCT115 colon, PC-9 lung, DLD-1 colon and WiDr colon models)^[1].
Dosage:	12.5, 25, 50 (100) mg/kg.
Administration:	IV daily for 4 days.
Result:	Exhibited anti-tumor activity.

Clinical Trial

Molecular Weight

385.85

Formula

C₁₄H₁₂ClN₃O₄S₂

CAS No.

165668-41-7

Appearance

Solid

Color

White to pink

SMILES

O=S(C1=CC=C(S(=O)(N)=O)C=C1)(NC2=CC=CC3=C2NC=C3Cl)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	1 year
	-20°C	6 months

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (259.17 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5917 mL	12.9584 mL	25.9168 mL
5 mM	0.5183 mL	2.5917 mL	5.1834 mL
10 mM	0.2592 mL	1.2958 mL	2.5917 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.17 mg/mL (5.62 mM); Clear solution

This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.17 mg/mL (5.62 mM); Clear solution

This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.17 mg/mL (5.62 mM); Clear solution

This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.80%

Select Batch:

Data Sheet (279 KB) SDS (393 KB)

COA (254 KB) HNMR (263 KB) LCMS (87 KB)

Handling Instructions (2659 KB)

References

[1]. Ozawa Y, et al. E7070, a novel sulphonamide agent with potent antitumour activity in vitro and in vivo. Eur J Cancer. 2001 Nov;37(17):2275-82. [Content Brief]

[2]. Abbate F, et al. Carbonic anhydrase inhibitors: E7070, a sulfonamide anticancer agent, potently inhibits cytosolic isozymes I and II, and transmembrane, tumor-associated isozyme IX. Bioorg Med Chem Lett. 2004 Jan 5;14(1):217-23. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.5917 mL	12.9584 mL	25.9168 mL	64.7920 mL
	5 mM	0.5183 mL	2.5917 mL	5.1834 mL	12.9584 mL
	10 mM	0.2592 mL	1.2958 mL	2.5917 mL	6.4792 mL
	15 mM	0.1728 mL	0.8639 mL	1.7278 mL	4.3195 mL
	20 mM	0.1296 mL	0.6479 mL	1.2958 mL	3.2396 mL
	25 mM	0.1037 mL	0.5183 mL	1.0367 mL	2.5917 mL
	30 mM	0.0864 mL	0.4319 mL	0.8639 mL	2.1597 mL
	40 mM	0.0648 mL	0.3240 mL	0.6479 mL	1.6198 mL
	50 mM	0.0518 mL	0.2592 mL	0.5183 mL	1.2958 mL
	60 mM	0.0432 mL	0.2160 mL	0.4319 mL	1.0799 mL
	80 mM	0.0324 mL	0.1620 mL	0.3240 mL	0.8099 mL
	100 mM	0.0259 mL	0.1296 mL	0.2592 mL	0.6479 mL

Indisulam Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Indisulam165668-41-7E 7070E7070E-7070Molecular GluesCarbonic AnhydraseCarbonate dehydrataseInhibitorinhibitorinhibit

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Indisulam (Synonyms: E 7070)

Customer Review

Other Forms of Indisulam:

Indisulam Related Antibodies

15 Publications Citing Use of MCE Indisulam

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Carbonic Anhydrase Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Indisulam Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Indisulam Related Classifications

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