2. Metabolic Enzyme/Protease
    Apoptosis
  3. Proteasome
    Apoptosis
  4. MG-101

MG-101  (Synonyms: Calpain inhibitor I; Ac-LLnL-CHO; ALLN)

Cat. No.: HY-18964 Purity: ≥98.0%
COA Handling Instructions

MG-101 (ALLN) is an inhibitor of cysteine proteases which inhibits calpain I, calpain II, cathepsin B and cathepsin L with Kis of 190, 220, 150 and 500 pM, respectively. MG-101 induces apoptosis and inhibits tumor growth, it can be used for the research of colon cancer.

For research use only. We do not sell to patients.

MG-101 Chemical Structure

MG-101 Chemical Structure

CAS No. : 110044-82-1

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 66 In-stock
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
 USD 66 In-stock
Solid
5 mg USD 60 In-stock
10 mg USD 90 In-stock
25 mg USD 190 In-stock
50 mg USD 300 In-stock
100 mg USD 480 In-stock
200 mg   Get quote  
500 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

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Based on 8 publication(s) in Google Scholar

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Description

MG-101 (ALLN) is an inhibitor of cysteine proteases which inhibits calpain I, calpain II, cathepsin B and cathepsin L with Kis of 190, 220, 150 and 500 pM, respectively. MG-101 induces apoptosis and inhibits tumor growth, it can be used for the research of colon cancer[1].

In Vitro

MG-101 (0-26 μM; 24 h) affects the growth of colon cancer HCT116 cells[1].
MG-101 (0-26 μM; 24 h) induces apoptosis of various cancer cells[1].
MG-101 (0-26 μM; 24 h) induces Bax-dependent apoptosis in HCT116 cells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: HCT116 cell line
Concentration: 0-26 μM
Incubation Time: 24 hours
Result: Anchorage-independently and significantly decreased the viability of HCT116 cells.

Western Blot Analysis[1]

Cell Line: HCT116, RKO, SW480 and HepG2 cell lines
Concentration: 0-26 μM
Incubation Time: 24 hours
Result: Dose-dependently decreased the concentration of pro-caspase 3 and increased the concentration of cleaved-PARP in HCT116 cells. Induced apoptosis of HCT116, RKO, SW480 and HepG2 cells.

Western Blot Analysis[1]

Cell Line: HCT116, HCT116/p53-/- and HCT116/Bax-/- cell lines
Concentration: 0-26 μM
Incubation Time: 24 hours
Result: Induced apoptosis via a Bax-dependent manner, but not via a p53-dependent manner.
In Vivo

MG-101 (10 mg/kg; i.p. once daily for 15 days) inhibits tumor growth in nude mice[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female athymic nude mice with HCT116 cells injection[1]
Dosage: 10 mg/kg
Administration: Intraperitoneal injection; 10 mg/kg once daily; for 15 days
Result: Significantly decreased tumor weight and volume of treated mice at day 26 compared to control mice, and showed no effect on body weights.
Molecular Weight

383.53

Appearance

Solid

Formula

C20H37N3O4
CAS No.
SMILES

O=C(N[[email protected]](C=O)CCCC)[[email protected]@H](NC([[email protected]@H](NC(C)=O)CC(C)C)=O)CC(C)C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 16.67 mg/mL (43.46 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6074 mL 13.0368 mL 26.0736 mL
5 mM 0.5215 mL 2.6074 mL 5.2147 mL
10 mM 0.2607 mL 1.3037 mL 2.6074 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 1.67 mg/mL (4.35 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: 1.67 mg/mL (4.35 mM); Suspended solution; Need ultrasonic

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 1.67 mg/mL (4.35 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: ≥98.0%

COA (250 KB) HNMR (234 KB)

Handling Instructions (2659 KB)
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Keywords:

MG-101110044-82-1Calpain inhibitor I Ac-LLnL-CHO ALLNMG101MG 101ProteasomeApoptosisanti-tumorcolon cancerapoptosisBaxmitochondriaHCT116RKOSW480HepG2Inhibitorinhibitorinhibit

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