Cat. No.: HY-107854

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N-Acetyl-5-hydroxytryptamine

(Synonyms: N-Acetylserotonin; Normelatonin; O-Demethylmelatonin)

2 Cited Publications

Customer Review

Based on 2 publication(s) in Google Scholar

N-Acetyl-5-hydroxytryptamine is a Melatonin precursor, and that it can potently activate TrkB receptor.

For research use only. We do not sell to patients.

Purity: 99.96%
CAS No.: 1210-83-9
Formula: C₁₂H₁₄N₂O₂
Molecular Weight:218.26

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

N-Acetyl-5-hydroxytryptamine Chemical Structure

CAS No.: 1210-83-9

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Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (2)
Solvent & Solubility
- In Vitro
- In Vivo
Protocol
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

N-Acetyl-5-hydroxytryptamine is a Melatonin precursor, and that it can potently activate TrkB receptor.

IC₅₀ & Target^[1]

Human Endogenous Metabolite

TrkB

All Endogenous Metabolite Isoforms :

All Trk Receptor Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
B16	IC₅₀	>30 μM Compound: 16	Antimelanogenic activity in mouse B16 cells assessed as inhibition of intracellular melanin accumulation after 2 days Antimelanogenic activity in mouse B16 cells assessed as inhibition of intracellular melanin accumulation after 2 days	[PMID: 19524439]
B16	IC₅₀	311 μM Compound: 16	Inhibition of catecholase activity of tyrosinase in mouse B16 cells assessed as dopachrome formation Inhibition of catecholase activity of tyrosinase in mouse B16 cells assessed as dopachrome formation	[PMID: 19524439]

In Vitro

N-Acetyl-5-hydroxytryptamine (NAS), a precursor of Melatonin, is acetylated from serotonin by AANAT (arylalkylamine N-acetyltransferase). N-acetylserotonin activates TrkB receptor in a circadian rhythm. N-Acetyl-5-hydroxytryptamine swiftly activates TrkB in a circadian manner and exhibits antidepressant effect in a TrkB-dependent manner. N-Acetyl-5-hydroxytryptamine rapidly activates TrkB, but not TrkA or TrkC, in a neurotrophin- and MT3 receptor-independent manner^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

To explore whether N-Acetyl-5-hydroxytryptamine, can trigger TrkB activation in vivo, TrkB F616A knockin mice are employed, where it has been shown that TrkB F616A activation can be selectively blocked by 1NMPP1, a derivative of kinase inhibitor PP1, leading to TrkB-null phenotypes. To assess whether N-Acetyl-5-hydroxytryptamine can mimic BDNF, cortical neurons from TrkB F616A knockin mice are prepared. In alignment with a previous report, BDNF- and NAS-mediated TrkB phosphorylation are selectively reduced by 1NMPP1 but not by K252a, whereas serotonin or Melatonin had no effect . These findings suggest that NAS strongly provokes both wild-type TrkB and TrkB F616A tyrosine phosphorylation and activation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 1210-83-9
Appearance Solid
Molecular Weight 218.26
Formula C₁₂H₁₄N₂O₂
Color White to light brown
SMILES

CC(NCCC1=CNC2=C1C=C(O)C=C2)=O
Synonyms

N-Acetylserotonin; Normelatonin; O-Demethylmelatonin
Structure Classification
- Alkaloids
- Indole Alkaloids
- Phenols
- Monophenols
Initial Source
- Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (2)

All RT-PCRIn Vivo Efficacy StudyELISA

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Journal Impact Factor

Diabetes

Gut microbiota-tryptophan metabolism-GLP-1 axis participates in β-cell regeneration induced by dapagliflozin. [Abstract]

2024 Jun 1;73(6):926-940. PMID: 38471012

N-Acetyl-5-hydroxytryptamine purchased from MedChemExpress. Usage Cited in: Diabetes. 2024 Jun 1;73(6):926-940. [Abstract]

Relative Gcg and Pcsk1 mRNA levels in the intestinal L-cell line STC1 cells incubated with different concentrations of N-Acetyl-5-hydroxytryptamine (N-acetylserotonin, 1-1000 μM) for 24 h.

N-Acetyl-5-hydroxytryptamine purchased from MedChemExpress. Usage Cited in: Diabetes. 2024 Jun 1;73(6):926-940. [Abstract]

GLP-1 secretion in STC1 cells cultured with different concentrations of indoleacetate or N-Acetyl-5-hydroxytryptamine (N-acetylserotonin, 1-1000 μM) for 60 min.
Phytother Res

Hypoglycemic Effect of Ginsenoside Compound K Mediated by N-Acetylserotonin Derived From Gut Microbiota. [Abstract]

2025 Jan 6. PMID: 39757809

N-Acetyl-5-hydroxytryptamine purchased from MedChemExpress. Usage Cited in: Phytother Res. 2025 Jan 6. [Abstract]

OGTT results showed that N-Acetyl-5-hydroxytryptamine (NAS, 5 mg/kg; p.o.; once daily for 4 weeks) treatment improved the blood glucose values at each time point, with AUC reduced by up to 35.5% in HFD-induced diabetic mice.

N-Acetyl-5-hydroxytryptamine purchased from MedChemExpress. Usage Cited in: Phytother Res. 2025 Jan 6. [Abstract]

N-Acetyl-5-hydroxytryptamine (NAS, 5-50 mM; 2 h) facilitated GLP-1 release from NCI-H716 cells stimulated with glucose in a concentration-increasing manner.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (458.17 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.5817 mL

22.9085 mL

45.8169 mL

5 mM

0.9163 mL

4.5817 mL

9.1634 mL

10 mM

0.4582 mL

2.2908 mL

4.5817 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (11.45 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (9.53 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (9.53 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protocol

Animal Administration^[1]

Mice^[1]
Two-month-old TrkB F616A mice are pretreated with 1NMPP1 in drinking water (50 μM) 1 day before the experiment, followed by administration of N-Acetyl-5-hydroxytryptamine (20 mg/kg, i.p.) or Melatonin (1 mg/kg, i.p.). Mice are killed at 1 h. The brain homogenates are analyzed by immunoblotting with anti-p-TrkB. Two- to three-month-old BDNF forebrain conditional knockout mice are injected i.p. with N-Acetyl-5-hydroxytryptamine or Melatonin. Mice are killed at 0, 0.5, 1, or 2 h following drug administration. Brain lysates are prepared and analyzed by immunoblotting with anti-phospho-TrkB Y816^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Jang SW, et al. N-acetylserotonin activates TrkB receptor in a circadian rhythm. Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3876-81. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	4.5817 mL	22.9085 mL	45.8169 mL	114.5423 mL
	5 mM	0.9163 mL	4.5817 mL	9.1634 mL	22.9085 mL
	10 mM	0.4582 mL	2.2908 mL	4.5817 mL	11.4542 mL
	15 mM	0.3054 mL	1.5272 mL	3.0545 mL	7.6362 mL
	20 mM	0.2291 mL	1.1454 mL	2.2908 mL	5.7271 mL
	25 mM	0.1833 mL	0.9163 mL	1.8327 mL	4.5817 mL
	30 mM	0.1527 mL	0.7636 mL	1.5272 mL	3.8181 mL
	40 mM	0.1145 mL	0.5727 mL	1.1454 mL	2.8636 mL
	50 mM	0.0916 mL	0.4582 mL	0.9163 mL	2.2908 mL
	60 mM	0.0764 mL	0.3818 mL	0.7636 mL	1.9090 mL
	80 mM	0.0573 mL	0.2864 mL	0.5727 mL	1.4318 mL
	100 mM	0.0458 mL	0.2291 mL	0.4582 mL	1.1454 mL

Keywords

N-Acetyl-5-hydroxytryptamine

2 Cited Publications

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N-Acetyl-5-hydroxytryptamine

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Publications Citing Use of MedChemExpress (MCE) N-Acetyl-5-hydroxytryptamine

All Endogenous Metabolite Isoforms

All Trk Receptor Isoforms

Biological Activity

Chemical Information

Publications (2)

Solvent & Solubility

Protocol

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

N-Acetyl-5-hydroxytryptamine Related Classifications

HY-107854 Related Classifications