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NB-598 Maleate 

Cat. No.: HY-16343C Purity: 99.10%
Handling Instructions

NB-598 Maleate is a potent and competitive inhibitor of squalene epoxidase (SE), and suppresses triglyceride biosynthesis through the farnesol pathway.

For research use only. We do not sell to patients.

NB-598 Maleate Chemical Structure

NB-598 Maleate Chemical Structure

CAS No. : 155294-62-5

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Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 299 In-stock
Estimated Time of Arrival: December 31
5 mg USD 240 In-stock
Estimated Time of Arrival: December 31
10 mg USD 360 In-stock
Estimated Time of Arrival: December 31
50 mg USD 1100 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of NB-598 Maleate:

Top Publications Citing Use of Products

    NB-598 Maleate purchased from MCE. Usage Cited in: J Steroid Biochem Mol Biol. 2015 Apr 9;152:34-44.

    GC/MS quantitation of squalene (A) and cholesterol (B) extracted from confluent 6-well plates cell layers from SEBO662 AR+ treated or not (Ctrl) with 10 nM DHT, in the presence or not of the squalene epoxidase inhibitor NB-598 (10 μM). Treatment time is 7 days. Squalene is only detected in NB-598-treated cell layers. DHT significantly increases squalene without significantly modifying cholesterol.
    • Biological Activity

    • Protocol

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    • References

    • Customer Review

    Description

    NB-598 Maleate is a potent and competitive inhibitor of squalene epoxidase (SE), and suppresses triglyceride biosynthesis through the farnesol pathway.

    In Vitro

    NB-598 (10 μM) causes a 36±7% reduction in total cholesterol level of MIN6 cells. NB-598 causes a significant decrease in cholesterol by 49±2%, 46±7%, and 48±2% from PM, ER, and SG, respectively. NB598 at concentrations up to 10 μM does not affect peak outward KV currents or the voltage dependence of activation but increases current inactivation[1]. NB-598 (10 μM) inhibits the synthesis of sterol and sterol ester from [14C]acetate without affecting the synthesis of other lipids such as phospholipids (PL), free fatty acids (FFA) and triacylglycerol (TG). In the absence of exogenous liposomal cholesterol, NB-598 reduces ACAT activity by 31%. NB-598 reduces ACAT activity by 22% even in the presence of a 600 PM concentration of liposomal cholesterol[2]. NB-598 suppresses the secretion of cholesterol and triacylglycerol from HepG2 cells into the medium[3].

    Molecular Weight

    565.74

    Formula

    C₃₁H₃₅NO₅S₂

    CAS No.

    155294-62-5

    SMILES

    O=C(O)/C=C\C(O)=O.CC(C)(C)C#C/C=C/CN(CC)CC1=CC(OCC2=CC(C3=CSC=C3)=CS2)=CC=C1

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    4°C, stored under nitrogen

    *In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (176.76 mM; Need ultrasonic)

    H2O : < 0.1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.7676 mL 8.8380 mL 17.6760 mL
    5 mM 0.3535 mL 1.7676 mL 3.5352 mL
    10 mM 0.1768 mL 0.8838 mL 1.7676 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (4.42 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: 2.5 mg/mL (4.42 mM); Suspended solution; Need ultrasonic

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (4.42 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
    Kinase Assay
    [2]

    Caco-2 cells are grown in a 58-cm2 plastic dish with medium A for 13 days. The cells are washed with medium B, and then cultured with medium B including cholesterol-micelle and each compound. The compound is dissolved in Me2SO, and the final concentration of Me2SO is 0.1%(v/v). After 18 hr of incubation, the cells are washed extensively with phosphate-buffered saline (PBS) to remove the compound. Microsomes are prepared as described above. The reaction mixture (0.2 mL) consisted of 0.1 mg microsomes, 0.25% BSA and 40 PM [14C]oleoyl CoA in buffer A. To avoid the effects of endogenous cholesterol, liposome (2 mol of cholesterol: 1 mol of phosphatidylcholine) [15] is added to the reaction mixture. The microsomes are preincubated for 1 hr with or without exogenous cholesterol, and ACAT activity is determined as described above.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References

    Purity: 99.10%

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    Keywords:

    NB-598NB598NB 598OthersInhibitorinhibitorinhibit

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    Product name:
    NB-598 Maleate
    Cat. No.:
    HY-16343C
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