Cat. No.: HY-10405

Pamapimod

(Synonyms: Ro4402257; R1503)

1 Cited Publications

Customer Review

Based on 1 publication(s) in Google Scholar

Pamapimod (Ro4402257) is a potent, selective and orally active p38 MAPK inhibitor with IC₅₀s of 14 nM and 480 nM and K_is of 1.3 nM and 120 nM for p38α and p38β, respectively. Pamapimod has no activity against p38δ or p38γ isoforms. Pamapimod has the potential for rheumatoid arthritis and other autoimmune diseases treatment.

For research use only. We do not sell to patients.

Purity: 99.71%
CAS No.: 449811-01-2
Formula: C₁₉H₂₀F₂N₄O₄
Molecular Weight:406.38

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Pamapimod Chemical Structure

CAS No.: 449811-01-2

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (1)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

p38α

14 nM (IC₅₀)

p38α

1.3 nM (Ki)

p38β

480 (IC₅₀)

p38β

120 nM (Ki)

All p38 MAPK Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	IC₅₀	>30 μM Compound: 5	Cytotoxicity against HEK293 after 24 hrs by CellTiter-Glo luminescent cell viability assay Cytotoxicity against HEK293 after 24 hrs by CellTiter-Glo luminescent cell viability assay	[PMID: 26704265]
THP-1	IC₅₀	0.025 μM Compound: 2b, Pamapimod	Inhibition of LPS-induced TNFalpha production in human THP1 cells after 2 hrs by ELISA Inhibition of LPS-induced TNFalpha production in human THP1 cells after 2 hrs by ELISA	[PMID: 21375264]
THP-1	IC₅₀	25 nM Compound: 1	Inhibition of LPS-induced TNFalpha production in human THP1 cells by ELISA Inhibition of LPS-induced TNFalpha production in human THP1 cells by ELISA	[PMID: 19950901]

In Vitro

Pamapimod binds to JNK kinases with K_i values of 190 nM, 16 nM and 19 nM for Jnk1, Jnk2 and Jnk3, respectively^[1].
After lipopolysaccharide (LPS) stimulation of the human myelomonocytic cell line (THP-1), secretion of TNF-α is inhibited by Pamapimod, with an EC₅₀ of 25 nM. Pamapimod suppresses TNF-α and IL-1β production in whole blood, with EC₅₀ values of 0.40 and 0.10 μM, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Pamapimod (0-150 mg/kg; oral gavage; once daily; DBA/1J female mice) treatment reduces inflammation and bone loss in murine collagen-induced arthritis^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

DBA/1J female mice (8-10 weeks of age) induced murine collagen^[1]
Dosage:

0 mg/kg, 3 mg/kg, 10 mg/kg, 30 mg/kg, 90 mg/kg, 150 mg/kg
Administration:

Oral gavage; once daily
Result:

Reduced inflammation and bone loss.

Chemical Information

CAS No. 449811-01-2
Appearance Solid
Molecular Weight 406.38
Formula C₁₉H₂₀F₂N₄O₄
Color White to off-white
SMILES

O=C1C(OC2=CC=C(F)C=C2F)=CC3=CN=C(NC(CCO)CCO)N=C3N1C
Synonyms

Ro4402257; R1503
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (1)

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Journal Impact Factor

Nat Commun

PRL3-zumab as an anti-angiogenic therapy in neovascular eye diseases. [Abstract]

2025 May 23;16(1):4791. PMID: 40410189

Solvent & Solubility

In Vitro:

DMSO : ≥ 34 mg/mL (83.67 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.4608 mL

12.3038 mL

24.6075 mL

5 mM

0.4922 mL

2.4608 mL

4.9215 mL

10 mM

0.2461 mL

1.2304 mL

2.4608 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Hill, R. J. et al. Pamapimod, a novel p38 mitogen-activated protein kinase inhibitor: preclinical analysis of efficacy and selectivity. The Journal of pharmacology and experimental therapeutics 327, 610-619, doi:10.1124/jpet.108.139006 (2008). [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.4608 mL	12.3038 mL	24.6075 mL	61.5188 mL
	5 mM	0.4922 mL	2.4608 mL	4.9215 mL	12.3038 mL
	10 mM	0.2461 mL	1.2304 mL	2.4608 mL	6.1519 mL
	15 mM	0.1641 mL	0.8203 mL	1.6405 mL	4.1013 mL
	20 mM	0.1230 mL	0.6152 mL	1.2304 mL	3.0759 mL
	25 mM	0.0984 mL	0.4922 mL	0.9843 mL	2.4608 mL
	30 mM	0.0820 mL	0.4101 mL	0.8203 mL	2.0506 mL
	40 mM	0.0615 mL	0.3076 mL	0.6152 mL	1.5380 mL
	50 mM	0.0492 mL	0.2461 mL	0.4922 mL	1.2304 mL
	60 mM	0.0410 mL	0.2051 mL	0.4101 mL	1.0253 mL
	80 mM	0.0308 mL	0.1538 mL	0.3076 mL	0.7690 mL

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Pamapimod

Immunology/Inflammation Compound Library

Kinase Inhibitor Library

MAPK Compound Library

FDA-Approved Drug Library

Autophagy Compound Library

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Publications Citing Use of MedChemExpress (MCE) Pamapimod

All p38 MAPK Isoforms

Biological Activity

Chemical Information

Publications (1)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Pamapimod Related Classifications

Cat. No.: HY-10405