Podocarpusflavone A

Name: Podocarpusflavone A
Brand: MedChemExpress
SKU: HY-N2198
Rating: 4.5 (0 reviews)

Cat. No.: HY-N2198 Purity: 99.62%: Data Sheet
COA

SDS
Handling Instructions
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Podocarpusflavone A is a biflavonoid present in the leaves of Podocarpus henkelii, with anti-tumor, topoisomerase I inhibitory, antibacterial and antifungal activities. Podocarpusflavone A exhibits antibacterial activity against Enterococcus faecalis and Pseudomonas aeruginosa. Podocarpusflavone A shows weak activity against fungal pathogens. Podocarpusflavone A targets topoisomerase I and induces cell cycle arrest and apoptosis in MCF-7 cells. Podocarpusflavone A can be used for studies on cancer, bacterial and fungal infections.

For research use only. We do not sell to patients.

Podocarpusflavone A Chemical Structure

CAS No. : 22136-74-9

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	Get quote
50 mg	Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[2]

Topoisomerase I

65.98 μg/mL (IC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	1.6 μg/mL Compound: 2	Cytotoxicity against A549 cells Cytotoxicity against A549 cells	[PMID: 17027271]
B16	IC₅₀	1.6 μg/mL Compound: 2	Cytotoxicity against B16 cells Cytotoxicity against B16 cells	[PMID: 17027271]
BGC-823	IC₅₀	1.6 μg/mL Compound: 2	Cytotoxicity against BGC823 cells Cytotoxicity against BGC823 cells	[PMID: 17027271]
BHK-21	IC₅₀	0.75 μM Compound: 12	Cytotoxicity against hamster BHK-21 cells assessed as reduction in cell viability incubated for 24 hrs by Celltiter assay Cytotoxicity against hamster BHK-21 cells assessed as reduction in cell viability incubated for 24 hrs by Celltiter assay	[PMID: 34274831]
Bel-7402	IC₅₀	1.6 μg/mL Compound: 2	Cytotoxicity against BEL-7402 cells Cytotoxicity against BEL-7402 cells	[PMID: 17027271]
DU-145	IC₅₀	1.6 μg/mL Compound: 2	Cytotoxicity against DU145 cells Cytotoxicity against DU145 cells	[PMID: 17027271]
HT-29	IC₅₀	1.6 μg/mL Compound: 2	Cytotoxicity against HT29 cells Cytotoxicity against HT29 cells	[PMID: 17027271]
HT-29	IC₅₀	7.9 μM Compound: Podocarpusflavone A	Antiproliferative activity against human HT-29 cells assessed as decrease in cell viability after 24 hrs by CCK8 assay Antiproliferative activity against human HT-29 cells assessed as decrease in cell viability after 24 hrs by CCK8 assay	[PMID: 30108705]
L6	IC₅₀	0.75 μM Compound: 12	Cytotoxicity against African green monkey COS-7 cells assessed as reduction in cell viability incubated for 24 hrs by Celltiter assay Cytotoxicity against African green monkey COS-7 cells assessed as reduction in cell viability incubated for 24 hrs by Celltiter assay	[PMID: 34274831]
MCF7	IC₅₀	1.6 μg/mL Compound: 2	Cytotoxicity against MCF7 cells Cytotoxicity against MCF7 cells	[PMID: 17027271]
MCF7	IC₅₀	35.1 μM Compound: 5	Cytotoxicity against human MCF7 cells measured after 24 hrs by MTT assay Cytotoxicity against human MCF7 cells measured after 24 hrs by MTT assay	[PMID: 34875389]
MDA-MB-231	IC₅₀	1.6 μg/mL Compound: 2	Cytotoxicity against MDA-MB-231 cells Cytotoxicity against MDA-MB-231 cells	[PMID: 17027271]
PC-3	GI₅₀	> 100 μM Compound: 17	Growth inhibition of human PC3 cells by colorimetric MTT assay Growth inhibition of human PC3 cells by colorimetric MTT assay	[PMID: 25736997]
SGC-7901	IC₅₀	1.6 μg/mL Compound: 2	Cytotoxicity against SGC7901 cells Cytotoxicity against SGC7901 cells	[PMID: 17027271]

In Vitro

Podocarpusflavone A (PFA) (Serial two-fold dilutions from an initial 1 mg/mL; 12-24 h) shows broad-spectrum bactericidal activity against Enterococcus faecalis and Pseudomonas aeruginosa (MIC = 60 μg/mL), with weaker activity against Staphylococcus aureus (MIC = 130 μg/mL) and Escherichia coli (MIC = 250 μg/mL)^[1].
Podocarpusflavone A (Serial two-fold dilutions from an initial 1 mg/mL; 24-48 h) exhibits weak antifungal activity against Candida albicans and Aspergillus fumigatus (MIC = 250 μg/mL) and Cryptococcus neoformans (MIC = 130 μg/mL) at 24 h and 48 h incubation^[1].
Podocarpusflavone A (CC₅₀ threshold of 1000 μg/mL; MIC values as measured in antibacterial and antifungal assays) has favorable selective antimicrobial activity (SI > 15) against Enterococcus faecalis and Pseudomonas aeruginosa, with lower selectivity against other tested bacterial and fungal pathogens^[1].
Podocarpusflavone A (PF) (1-40 µM; 72 h) shows potent antiproliferative activity against KB, MCF-7, DLD and HEp-2 tumor cell lines with ED₅₀ values of 4.67 µg/mL, 15.17 µg/mL, 4.95 µg/mL and 10.87 µg/mL^[2].

Podocarpusflavone A (20-40 µg/mL; 24 h) induces cell cycle arrest at sub-G1/S phase and triggers apoptosis in MCF-7 cells^[2].
Podocarpusflavone A shows moderate Topoisomerase I inhibitory activity with an IC₅₀ value of 65.98 µg/mL^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cycle Analysis^[2]

Cell Line:	MCF-7 cells
Concentration:	20, 40 µg/mL
Incubation Time:	24 h
Result:	Induced cell cycle arrest at sub-G1/S phase in MCF-7 cells.

Apoptosis Analysis^[2]

Cell Line:	MCF-7 cells
Concentration:	20, 40 µg/mL
Incubation Time:	24 h
Result:	Induced apoptosis in MCF-7 cells.

Molecular Weight

552.48

Formula

C₃₁H₂₀O₁₀

CAS No.

22136-74-9

Appearance

Solid

Color

Light yellow to green yellow

SMILES

O=C1C=C(C2=CC=C(OC)C=C2)OC3=C(C4=CC(C5=CC(C6=C(O)C=C(O)C=C6O5)=O)=CC=C4O)C(O)=CC(O)=C13

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (90.50 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.8100 mL	9.0501 mL	18.1002 mL
5 mM	0.3620 mL	1.8100 mL	3.6200 mL
10 mM	0.1810 mL	0.9050 mL	1.8100 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (2.26 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.62%

Select Batch:

Data Sheet (294 KB) SDS (393 KB)

COA (260 KB) HNMR (279 KB) RP-HPLC (251 KB) MS (69 KB)

Handling Instructions (2659 KB)

References

[1]. Bagla VP, et al. Antimicrobial activity, toxicity and selectivity index of two biflavonoids and a flavone isolated from Podocarpus henkelii (Podocarpaceae) leaves. BMC Complement Altern Med. 2014;14:383. Published 2014 Oct 8.

[2]. Yeh PH, et al. Naturally Occurring Cytotoxic [3′→8″]-Biflavonoids from Podocarpus nakaii. Journal of Traditional and Complementary Medicine. 2011;2(3):220-226. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.8100 mL	9.0501 mL	18.1002 mL	45.2505 mL
	5 mM	0.3620 mL	1.8100 mL	3.6200 mL	9.0501 mL
	10 mM	0.1810 mL	0.9050 mL	1.8100 mL	4.5251 mL
	15 mM	0.1207 mL	0.6033 mL	1.2067 mL	3.0167 mL
	20 mM	0.0905 mL	0.4525 mL	0.9050 mL	2.2625 mL
	25 mM	0.0724 mL	0.3620 mL	0.7240 mL	1.8100 mL
	30 mM	0.0603 mL	0.3017 mL	0.6033 mL	1.5084 mL
	40 mM	0.0453 mL	0.2263 mL	0.4525 mL	1.1313 mL
	50 mM	0.0362 mL	0.1810 mL	0.3620 mL	0.9050 mL
	60 mM	0.0302 mL	0.1508 mL	0.3017 mL	0.7542 mL
	80 mM	0.0226 mL	0.1131 mL	0.2263 mL	0.5656 mL