Amentoflavone
Based on 19 publication(s) in Google Scholar
Amentoflavone (Didemethyl-ginkgetin) is a potent and orally active GABA(A) negative modulator. Amentoflavone also shows anti-inflammatory, antioxidative, anti-viral, anti-tumor, anti-radiation, anti-fungal, antibacterial activity. Amentoflavone induces apoptosis and cell cycle arrest at sub-G1 phase.
For research use only. We do not sell to patients.
- Purity: 99.66%
- CAS No.: 1617-53-4
- Formula: C30H18O10
- Molecular Weight:538.46
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
Publications Citing Use of MedChemExpress (MCE) Amentoflavone
More- Acta Pharm Sin B. 2021 Jan;11(1):143-155. [Abstract]
- Pharmacol Res. 2020 May;155:104751. [Abstract]
- Food Chem. 2025 Dec 30:497:146992. [Abstract]
- Food Chem. 2025 May 31:489:144992. [Abstract]
- Phytomedicine. 2023 Aug:117:154922. [Abstract]
- J Med Chem. 2021 Aug 26;64(16):12089-12108. [Abstract]
- Phytother Res. 2026 May;40(5):2380-2396. [Abstract]
- J Agric Food Res. 2026 Jan 16;26:102684.
- J Ethnopharmacol. 2025 Dec 16:359:121061. [Abstract]
- Life Sci. 2020 Apr 15;247:117425. [Abstract]
- J Photochem Photobiol B. 2025 Dec 16:274:113343. [Abstract]
- Naunyn Schmiedebergs Arch Pharmacol. 2024 Nov;397(11):8903-8916. [Abstract]
- Vet Microbiol. 2026 May:316:110992. [Abstract]
- S Afr J Bot. 2025 Sep.
- Cell Biochem Biophys. 2025 Mar;83(1):1299-1312. [Abstract]
- Sex Med. 2025 Aug 6;13(4):qfaf059. [Abstract]
- Anticancer Res. 2021 Mar;41(3):1357-1364. [Abstract]
- SSRN. 2026 Jun 9.
- Research Square Preprint. 2024 Mar 15.
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WB
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Cell Imaging/Staining
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Histological Imaging/Staining
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Cell Proliferation/Viability Assay
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IF
Biological Activity
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Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
1.6 μg/mL
Compound: 1
|
Cytotoxicity against A549 cells
Cytotoxicity against A549 cells
|
[PMID: 17027271] |
| A549 | IC50 |
8.36 μM
Compound: 10
|
Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay
Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay
|
[PMID: 29921475] |
| B16 | IC50 |
1.6 μg/mL
Compound: 1
|
Cytotoxicity against B16 cells
Cytotoxicity against B16 cells
|
[PMID: 17027271] |
| Bel-7402 | IC50 |
1.6 μg/mL
Compound: 1
|
Cytotoxicity against BEL-7402 cells
Cytotoxicity against BEL-7402 cells
|
[PMID: 17027271] |
| BGC-823 | IC50 |
1.6 μg/mL
Compound: 1
|
Cytotoxicity against BGC823 cells
Cytotoxicity against BGC823 cells
|
[PMID: 17027271] |
| Calu-1 | IC50 |
>100 μM
Compound: 6
|
Cytotoxicity against human Calu1 cells after 3 days
Cytotoxicity against human Calu1 cells after 3 days
|
[PMID: 10843573] |
| DU-145 | IC50 |
1.6 μg/mL
Compound: 1
|
Cytotoxicity against DU145 cells
Cytotoxicity against DU145 cells
|
[PMID: 17027271] |
| Fibroblast | IC50 |
1.8 μM
Compound: 4
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Inhibition of UVB irradiation-induced MMP1 production in human dermal fibroblasts after 48 hrs
Inhibition of UVB irradiation-induced MMP1 production in human dermal fibroblasts after 48 hrs
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[PMID: 18029185] |
| HCT-15 | IC50 |
>50 μM
Compound: 8
|
Cytotoxicity against human HCT15 cells assessed as cell viability after 24 hrs by MTT assay
Cytotoxicity against human HCT15 cells assessed as cell viability after 24 hrs by MTT assay
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[PMID: 23206866] |
| HEK293 | IC50 |
23.8 μM
Compound: Amentoflavone
|
Inhibition of sPLA2-2A in HEK293 cell
Inhibition of sPLA2-2A in HEK293 cell
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[PMID: 16504502] |
| HEK293 | IC50 |
>100 μM
Compound: amentoflavone
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Inhibition of human cloned cPLA2 expressed in HEK293 cells
Inhibition of human cloned cPLA2 expressed in HEK293 cells
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[PMID: 17826099] |
| HEK293 | IC50 |
>100 μM
Compound: amentoflavone
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Inhibition of human cloned sPLA2-10 expressed in HEK293 cells
Inhibition of human cloned sPLA2-10 expressed in HEK293 cells
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[PMID: 17826099] |
| HEK293 | IC50 |
>100 μM
Compound: amentoflavone
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Inhibition of rat cloned sPLA2-1B expressed in HEK293 cells
Inhibition of rat cloned sPLA2-1B expressed in HEK293 cells
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[PMID: 17826099] |
| HEK293 | IC50 |
41.8 μM
Compound: amentoflavone
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Inhibition of human cloned sPLA2-5 expressed in HEK293 cells
Inhibition of human cloned sPLA2-5 expressed in HEK293 cells
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[PMID: 17826099] |
| HeLa | IC50 |
>100 μM
Compound: 6
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Cytotoxicity against human HeLa cells after 3 days
Cytotoxicity against human HeLa cells after 3 days
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[PMID: 10843573] |
| HeLa | IC50 |
46.4 μM
Compound: amentoflavone
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Cytotoxicity against human HeLa cells assessed as growth inhibition by sulforhodamine B assay
Cytotoxicity against human HeLa cells assessed as growth inhibition by sulforhodamine B assay
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[PMID: 26722868] |
| HeLa | IC50 |
5.3 μM
Compound: 7
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Antiproliferative activity against human HeLa cells after 48 hrs by CCK8 assay
Antiproliferative activity against human HeLa cells after 48 hrs by CCK8 assay
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[PMID: 29475587] |
| HeLa | IC50 |
>10 μM
Compound: 66
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Antiproliferative activity against human HeLa cells assessed as reduction in cell viability
Antiproliferative activity against human HeLa cells assessed as reduction in cell viability
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[PMID: 30830783] |
| HepG2 | IC50 |
>50 μM
Compound: 8
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Cytotoxicity against human HepG2 cells assessed as cell viability after 24 hrs by MTT assay
Cytotoxicity against human HepG2 cells assessed as cell viability after 24 hrs by MTT assay
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[PMID: 23206866] |
| HepG2 | IC50 |
9.13 μM
Compound: 10
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Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 48 hrs by MTT assay
Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 48 hrs by MTT assay
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[PMID: 29921475] |
| HL-60 | IC50 |
>50 μM
Compound: 8
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Cytotoxicity against human HL60 cells assessed as cell viability after 24 hrs by MTT assay
Cytotoxicity against human HL60 cells assessed as cell viability after 24 hrs by MTT assay
|
[PMID: 23206866] |
| HL-60 | IC50 |
51.2 μM
Compound: 7
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Antiproliferative activity against human HL60 cells after 48 hrs by CCK8 assay
Antiproliferative activity against human HL60 cells after 48 hrs by CCK8 assay
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[PMID: 29475587] |
| HT-29 | IC50 |
1.6 μg/mL
Compound: 1
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Cytotoxicity against HT29 cells
Cytotoxicity against HT29 cells
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[PMID: 17027271] |
| Huh-7 | CC50 |
>50 μM
Compound: 12
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Cytotoxicity against human Huh7.5.1 cells by MTT assay
Cytotoxicity against human Huh7.5.1 cells by MTT assay
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[PMID: 22445328] |
| Jurkat | IC50 |
>50 μM
Compound: 8
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Cytotoxicity against human Jurkat cells assessed as cell viability after 24 hrs by MTT assay
Cytotoxicity against human Jurkat cells assessed as cell viability after 24 hrs by MTT assay
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[PMID: 23206866] |
| K562 | IC50 |
>100 μM
Compound: 6
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Cytotoxicity against human K562 cells after 3 days
Cytotoxicity against human K562 cells after 3 days
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[PMID: 10843573] |
| MCF7 | IC50 |
1.6 μg/mL
Compound: 1
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Cytotoxicity against MCF7 cells
Cytotoxicity against MCF7 cells
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[PMID: 17027271] |
| MCF7 | IC50 |
75.4 μM
Compound: 4
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Cytotoxicity against human MCF7 cells measured after 24 hrs by MTT assay
Cytotoxicity against human MCF7 cells measured after 24 hrs by MTT assay
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[PMID: 34875389] |
| MDA-MB-231 | IC50 |
1.6 μg/mL
Compound: 1
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Cytotoxicity against MDA-MB-231 cells
Cytotoxicity against MDA-MB-231 cells
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[PMID: 17027271] |
| MGC-803 | IC50 |
9.86 μM
Compound: 10
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Cytotoxicity against human MGC803 cells assessed as reduction in cell viability after 48 hrs by MTT assay
Cytotoxicity against human MGC803 cells assessed as reduction in cell viability after 48 hrs by MTT assay
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[PMID: 29921475] |
| Monocyte | IC50 |
3 μM
Compound: amentoflavone
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Inhibition of procoagulant activity in monocyte from human blood assessed as counteraction of IL1-induced tissue factor expression after 18 hrs
Inhibition of procoagulant activity in monocyte from human blood assessed as counteraction of IL1-induced tissue factor expression after 18 hrs
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[PMID: 8882428] |
| Monocyte | IC50 |
3 x 10-6 M
Compound: amentoflavone
|
Inhibition of procoagulant activity in monocyte from human blood assessed as counteraction of IL1-induced tissue factor expression after 18 hrs
Inhibition of procoagulant activity in monocyte from human blood assessed as counteraction of IL1-induced tissue factor expression after 18 hrs
|
[PMID: 8882428] |
| Neutrophil | IC50 |
0.75 μM
Compound: 7
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Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced elastase release pretreated for 5 mins followed by fMLP/CB stimulation using MeO-Suc-Ala-Ala-Pro-Val-p-nitroanilide as substrate
Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced elastase release pretreated for 5 mins followed by fMLP/CB stimulation using MeO-Suc-Ala-Ala-Pro-Val-p-nitroanilide as substrate
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[PMID: 29381070] |
| Neutrophil | IC50 |
1.01 μM
Compound: 7
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Anti-inflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced superoxide anion generation pretreated for 5 mins followed by cytochalasin B addition for 3 mins and subsequent fMLP stimulation for 10 mins by superoxide dismutase
Anti-inflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced superoxide anion generation pretreated for 5 mins followed by cytochalasin B addition for 3 mins and subsequent fMLP stimulation for 10 mins by superoxide dismutase
|
[PMID: 29381070] |
| NIH3T3 | IC50 |
9.2 μM
Compound: Amentoflavone
|
Inhibition of PDGF-induced phosphotidyl inositol turnover in PLCgamma1 expressing mouse NIH3T3 cells
Inhibition of PDGF-induced phosphotidyl inositol turnover in PLCgamma1 expressing mouse NIH3T3 cells
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[PMID: 10869194] |
| Oocyte | EC50 |
3.6 μM
Compound: Amentoflavone
|
Effective concentration against human recombinant GABA-A receptor alpha-1-beta-2-gamma-2 subunits expressed in Xenopus oocytes
Effective concentration against human recombinant GABA-A receptor alpha-1-beta-2-gamma-2 subunits expressed in Xenopus oocytes
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[PMID: 12824018] |
| PBMC | IC50 |
35 μM
Compound: 1
|
Cytotoxicity against uninfected PHA-stimulated human PBMC assessed as thymidine incorporation after 24 hrs by trypan blue exclusion method
Cytotoxicity against uninfected PHA-stimulated human PBMC assessed as thymidine incorporation after 24 hrs by trypan blue exclusion method
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[PMID: 9322359] |
| Raji | IC50 |
>100 μM
Compound: 6
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Cytotoxicity against human Raji cells after 3 days
Cytotoxicity against human Raji cells after 3 days
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[PMID: 10843573] |
| SGC-7901 | IC50 |
1.6 μg/mL
Compound: 1
|
Cytotoxicity against SGC7901 cells
Cytotoxicity against SGC7901 cells
|
[PMID: 17027271] |
| SW480 | IC50 |
111 μM
Compound: 9
|
Cytotoxicity against human SW480 cells after 72 hrs by MTT assay
Cytotoxicity against human SW480 cells after 72 hrs by MTT assay
|
[PMID: 15787435] |
| T-24 | IC50 |
10.67 μM
Compound: 10
|
Cytotoxicity against human T24 cells assessed as reduction in cell viability after 48 hrs by MTT assay
Cytotoxicity against human T24 cells assessed as reduction in cell viability after 48 hrs by MTT assay
|
[PMID: 29921475] |
| THP-1 | IC50 |
>50 μM
Compound: 8
|
Cytotoxicity against human THP1 cells assessed as cell viability after 24 hrs by MTT assay
Cytotoxicity against human THP1 cells assessed as cell viability after 24 hrs by MTT assay
|
[PMID: 23206866] |
| U-937 | IC50 |
>50 μM
Compound: 8
|
Cytotoxicity against human U937 cells assessed as cell viability after 24 hrs by MTT assay
Cytotoxicity against human U937 cells assessed as cell viability after 24 hrs by MTT assay
|
[PMID: 23206866] |
| Vero | IC50 |
>100 μM
Compound: 6
|
Cytotoxicity against african green monkey Vero cells after 3 days
Cytotoxicity against african green monkey Vero cells after 3 days
|
[PMID: 10843573] |
| WISH | IC50 |
>100 μM
Compound: 6
|
Cytotoxicity against human Wish cells after 3 days
Cytotoxicity against human Wish cells after 3 days
|
[PMID: 10843573] |
Amentoflavone (1-60 μM) inhibits the production of nitric oxide in a concentration-dependent manner in RAW 264.7 cells[2].
Amentoflavone (50-200 μM) inhibits the viability of U-87 MG cells with IC50 value of 100 μM at 48 h[3].
Amentoflavone (0, 50, 100 μM; 48 h) induces apoptosis and cell cycle arrest at sub-G1 phase[3].
Amentoflavone (0, 50, 100 μM; 48 h) inhibits NF- B activation and decreases the expression of MCL1 and C-FLIP protein in U-87 MG cells[3].
Amentoflavone (0-32 μg/ml) shows antibacterial activity with MICs of 8, 4, 32, 8, 16, 8 μg/ml for E. faecium ATCC 19434, S. aureus ATCC 25923, S. mutans ATCC 3065, E. coli O-157 ATCC 25922, E. coli ATCC 43895, P. aeruginosa ATCC 27853, respectively[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:U-87 MG cells
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Concentration:0, 50, 75, 100, 200 µM
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Incubation Time:48 h
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Result:Significantly inhibited the viability of U-87 MG cells by 23-71% with an IC50 value of 100 µM at 48 h.
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Cell Line:U-87 MG cells
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Concentration:0, 50, 100 µM
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Incubation Time:48 h
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Result:Significantly induced the accumulation of cells in the sub-G1 population and increased the level of active caspase-3 by 14-52% and 24-42%, respectively, and significantly triggered the loss of Ψm and the expression of active caspase-8 by 23-53% and 25-50%, respectively.
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Cell Line:U-87 MG cells
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Concentration:0, 50, 100 µM
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Incubation Time:48 h
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Result:Significantly reduced NF-ĸB activation in a dose-dependent manner by 25-87% and reduced protein expression of MCL1 and C-FLIP by 50-80% and 38-57%, respectively.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:5-6 weeks, 28-32 g, kunming mice[1]
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Dosage:25 mg/kg
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Administration:P.o.; once a day for 3 consecutive days
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Result:Inhibited activation and nuclear translocation of NF-κB subunits p65, decreased IL-6 and IL-1β production and significantly decreased NO and prostaglandin E2 production.
Chemical Information
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CAS No. 1617-53-4
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Appearance Solid
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Molecular Weight 538.46
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Formula C30H18O10
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Color Light yellow to yellow
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SMILES
O=C1C=C(C2=CC=C(O)C=C2)OC3=C(C4=CC(C5=CC(C6=C(O)C=C(O)C=C6O5)=O)=CC=C4O)C(O)=CC(O)=C13
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Synonyms
Didemethyl-ginkgetin
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (19)
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Journal Impact Factor
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Most Recent
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Acta Pharm Sin B
Chrysin serves as a novel inhibitor of DGK α/FAK interaction to suppress the malignancy of esophageal squamous cell carcinoma (ESCC). [Abstract]2021 Jan;11(1):143-155. PMID: 33532186 -
Pharmacol Res
Cardamonin retards progression of autosomal dominant polycystic kidney disease via inhibiting renal cyst growth and interstitial fibrosis. [Abstract]2020 May;155:104751. PMID: 32151678 -
Food Chem
Effects of sun drying combined with baking processes on the flavor quality of Chongqing Tuocha raw tea. [Abstract]2025 Dec 30:497:146992. PMID: 41285060 -
Food Chem
Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]2025 May 31:489:144992. PMID: 40466530 -
Phytomedicine
Amentoflavone mitigates doxorubicin-induced cardiotoxicity by suppressing cardiomyocyte pyroptosis and inflammation through inhibition of the STING/NLRP3 signalling pathway. [Abstract]2023 Aug:117:154922. PMID: 37321078
Amentoflavone purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2023 Aug:117:154922. [Abstract]
Mice were subjected to a 2-week intraperitoneal injection of saline (vehicle) or DOX with or without the combined administration of Amentoflavone (AMF) (20 mg/kg) and/or nigericin, Representative western blot.
Amentoflavone purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2023 Aug:117:154922. [Abstract]
Mice were subjected to a 2-week intraperitoneal injection of saline (vehicle) or DOX with or without the combined administration of Amentoflavone (AMF) (20 mg/kg) and/or nigericin, Representative images of TUNEL staining.
Amentoflavone purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2023 Aug:117:154922. [Abstract]
Mice were subjected to a 2-week intraperitoneal injection of saline (vehicle) or DOX with/without combined administration of Amentoflavone (AMF) (20 mg/kg), Representative images of the hematoxylineosin (HE) staining .
Amentoflavone purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2023 Aug:117:154922. [Abstract]
Primary cardiomyocytes were subjected to saline (vehicle) or DOX (1 µM, 24 h) with/without combined incubation of Amentoflavone (AMF, 20 µM,24 h) and/or ABZI (1 µM, 3 h). Viability of cardiomyocytes was measured using the CCK-8 assay.
Amentoflavone purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2023 Aug:117:154922. [Abstract]
Primary cardiomyocytes were subjected to saline (vehicle) or DOX (1 µM, 24 h) with/without combined incubation of Amentoflavone (AMF, 20 µM,24 h) and/or ABZI (1 µM, 3 h). Representative immunofluorescence staining of GSDMD-N.
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J Med Chem
Discovery of Novel Apigenin-Piperazine Hybrids as Potent and Selective Poly (ADP-Ribose) Polymerase-1 (PARP-1) Inhibitors for the Treatment of Cancer. [Abstract]2021 Aug 26;64(16):12089-12108. PMID: 34404206 -
Phytother Res
Amentoflavone, a Preferentially TGF-β Receptor 1 Inhibitor, Alleviates Cardiac Remodeling and Dysfunction Through Modulating the TGF-β/Smad and MAPK Signaling Pathways. [Abstract]2026 May;40(5):2380-2396. PMID: 41684292 -
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J Ethnopharmacol
Amentoflavone from Ginkgo biloba inhibits EMT-driven lung cancer metastasis by targeting TGFBR2: Integrative network pharmacology, machine learning, and experimental validation. [Abstract]2025 Dec 16:359:121061. PMID: 41412234 -
Life Sci
Amentoflavone suppresses cell proliferation and induces cell death through triggering autophagy-dependent ferroptosis in human glioma. [Abstract]2020 Apr 15;247:117425. PMID: 32057904 -
J Photochem Photobiol B
Selaginella Tamariscina extract reduces UVA-induced skin photodamage via regulating apoptosis and autophagy by AKT phosphorylation. [Abstract]2025 Dec 16:274:113343. PMID: 41421059 -
Naunyn Schmiedebergs Arch Pharmacol
Amentoflavone alleviated cartilage injury and inflammatory response of knee osteoarthritis through PTGS2. [Abstract]2024 Nov;397(11):8903-8916. PMID: 38856914 -
Vet Microbiol
The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]2026 May:316:110992. PMID: 41865607 -
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Cell Biochem Biophys
Amentoflavone Induces Ferroptosis to Alleviate Proliferation, Migration, Invasion and Inflammation in Rheumatoid Arthritis Fibroblast-like Synoviocytes by Inhibiting PIN1. [Abstract]2025 Mar;83(1):1299-1312. PMID: 39354278 -
Sex Med
Hinokiflavone alleviates high-fat diet-induced erectile dysfunction via the EGFR/PI3K/Akt/eNOS signaling pathway. [Abstract]2025 Aug 6;13(4):qfaf059. PMID: 40808867 -
Anticancer Res
Amentoflavone Induces Cell-cycle Arrest, Apoptosis, and Invasion Inhibition in Non-small Cell Lung Cancer Cells. [Abstract]2021 Mar;41(3):1357-1364. PMID: 33788727 -
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Solvent & Solubility
DMSO : 100 mg/mL (185.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.64 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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-
-
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (288 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Zhang Z, et al. Amentoflavone protects hippocampal neurons: anti-inflammatory, antioxidative, and antiapoptotic effects. Neural Regen Res. 2015 Jul;10(7):1125-33. [Content Brief]
[2]. Woo ER, et al. Amentoflavone inhibits the induction of nitric oxide synthase by inhibiting NF-kappaB activation in macrophages. Pharmacol Res. 2005 Jun;51(6):539-46. [Content Brief]
[3]. Yen TH, et al. Amentoflavone Induces Apoptosis and Inhibits NF-ĸB-modulated Anti-apoptotic Signaling in Glioblastoma Cells. In Vivo. 2018 Mar-Apr;32(2):279-285. [Content Brief]
[4]. Hwang JH, et al. Antibacterial effect of amentoflavone and its synergistic effect with antibiotics. J Microbiol Biotechnol. 2013;23(7):953-8. [Content Brief]
[5]. Hanrahan JR, et al. Semisynthetic preparation of amentoflavone: A negative modulator at GABA(A) receptors. Bioorg Med Chem Lett. 2003 Jul 21;13(14):2281-4. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.8571 mL | 9.2857 mL | 18.5715 mL | 46.4287 mL |
| 5 mM | 0.3714 mL | 1.8571 mL | 3.7143 mL | 9.2857 mL | |
| 10 mM | 0.1857 mL | 0.9286 mL | 1.8571 mL | 4.6429 mL | |
| 15 mM | 0.1238 mL | 0.6190 mL | 1.2381 mL | 3.0952 mL | |
| 20 mM | 0.0929 mL | 0.4643 mL | 0.9286 mL | 2.3214 mL | |
| 25 mM | 0.0743 mL | 0.3714 mL | 0.7429 mL | 1.8571 mL | |
| 30 mM | 0.0619 mL | 0.3095 mL | 0.6190 mL | 1.5476 mL | |
| 40 mM | 0.0464 mL | 0.2321 mL | 0.4643 mL | 1.1607 mL | |
| 50 mM | 0.0371 mL | 0.1857 mL | 0.3714 mL | 0.9286 mL | |
| 60 mM | 0.0310 mL | 0.1548 mL | 0.3095 mL | 0.7738 mL | |
| 80 mM | 0.0232 mL | 0.1161 mL | 0.2321 mL | 0.5804 mL | |
| 100 mM | 0.0186 mL | 0.0929 mL | 0.1857 mL | 0.4643 mL |