2. Immunology/Inflammation
    NF-κB
    Metabolic Enzyme/Protease
    Apoptosis
    Anti-infection
    Neuronal Signaling
    Membrane Transporter/Ion Channel
  3. Reactive Oxygen Species
    Apoptosis
    Bacterial
    Fungal
    RSV
    GABA Receptor
  4. Amentoflavone

Amentoflavone  (Synonyms: Didemethyl-ginkgetin)

Cat. No.: HY-N0662 Purity: 99.72%
COA Handling Instructions

Amentoflavone (Didemethyl-ginkgetin) is a potent and orally active GABA(A) negative modulator. Amentoflavone also shows anti-inflammatory, antioxidative, anti-viral, anti-tumor, anti-radiation, anti-fungal, antibacterial activity. Amentoflavone induces apoptosis and cell cycle arrest at sub-G1 phase.

For research use only. We do not sell to patients.

Amentoflavone Chemical Structure

Amentoflavone Chemical Structure

CAS No. : 1617-53-4

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 91 In-stock
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
 USD 91 In-stock
Solid
5 mg USD 77 In-stock
10 mg USD 121 In-stock
25 mg USD 209 In-stock
50 mg USD 352 In-stock
100 mg USD 572 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 5 publication(s) in Google Scholar

Top Publications Citing Use of Products

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Description

Amentoflavone (Didemethyl-ginkgetin) is a potent and orally active GABA(A) negative modulator. Amentoflavone also shows anti-inflammatory, antioxidative, anti-viral, anti-tumor, anti-radiation, anti-fungal, antibacterial activity. Amentoflavone induces apoptosis and cell cycle arrest at sub-G1 phase[1][2][3][4].

In Vitro

Amentoflavone (1-60 µM) inhibits the production of nitric oxide in a concentration-dependent manner in RAW 264.7 cells[2].
Amentoflavone (50-200 µM) inhibits the viability of U-87 MG cells with IC50 value of 100 µM at 48 h[3].
Amentoflavone (0, 50, 100 µM; 48 h) induces apoptosis and cell cycle arrest at sub-G1 phase[3].
Amentoflavone (0, 50, 100 µM; 48 h) inhibits NF-ĸB activation and decreases the expression of MCL1 and C-FLIP protein in U-87 MG cells[3].
Amentoflavone (0-32 µg/ml) shows antibacterial activity with MICs of 8, 4, 32, 8, 16, 8 µg/ml for E. faecium ATCC 19434, S. aureus ATCC 25923, S. mutans ATCC 3065, E. coli O-157 ATCC 25922, E. coli ATCC 43895, P. aeruginosa ATCC 27853, respectively[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[3]

Cell Line: U-87 MG cells
Concentration: 0, 50, 75, 100, 200 µM
Incubation Time: 48 h
Result: Significantly inhibited the viability of U-87 MG cells by 23-71% with an IC50 value of 100 µM at 48 h.

Apoptosis Analysis[3]

Cell Line: U-87 MG cells
Concentration: 0, 50, 100 µM
Incubation Time: 48 h
Result: Significantly induced the accumulation of cells in the sub-G1 population and increased the level of active caspase-3 by 14-52% and 24-42%, respectively, and significantly triggered the loss of Ψm and the expression of active caspase-8 by 23-53% and 25-50%, respectively.

Western Blot Analysis[3]

Cell Line: U-87 MG cells
Concentration: 0, 50, 100 µM
Incubation Time: 48 h
Result: Significantly reduced NF-ĸB activation in a dose-dependent manner by 25-87% and reduced protein expression of MCL1 and C-FLIP by 50-80% and 38-57%, respectively.
In Vivo

Amentoflavone (25 mg/kg; p.o.; once a day for 3 consecutive days) shows neuroprotective role in epilepsy via anti-inflammatory effects in mouse[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 5-6 weeks, 28-32 g, kunming mice[1]
Dosage: 25 mg/kg
Administration: P.o.; once a day for 3 consecutive days
Result: Inhibited activation and nuclear translocation of NF-κB subunits p65, decreased IL-6 and IL-1β production and significantly decreased NO and prostaglandin E2 production.
Molecular Weight

538.46

Appearance

Solid

Formula

C30H18O10
CAS No.
SMILES

O=C1C=C(C2=CC=C(O)C=C2)OC3=C(C4=CC(C5=CC(C6=C(O)C=C(O)C=C6O5)=O)=CC=C4O)C(O)=CC(O)=C13
Structure Classification
Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (232.14 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8571 mL 9.2857 mL 18.5715 mL
5 mM 0.3714 mL 1.8571 mL 3.7143 mL
10 mM 0.1857 mL 0.9286 mL 1.8571 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (4.64 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: 2.5 mg/mL (4.64 mM); Suspended solution; Need ultrasonic

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 99.72%

COA (260 KB) HNMR (302 KB) LCMS (266 KB)

Handling Instructions (2659 KB)
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Keywords:

Amentoflavone1617-53-4Didemethyl-ginkgetinReactive Oxygen SpeciesApoptosisBacterialFungalRSVGABA ReceptorRespiratory syncytial virusGamma-aminobutyric acid Receptorγ-Aminobutyric acid Receptoranti-inflammatoryantibacterialapoptosisU-87 MG cellsNF-?BMCL1C-FLIPorally activeInhibitorinhibitorinhibit

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