1. Stem Cell/Wnt Apoptosis Autophagy
  2. Wnt Apoptosis Autophagy
  3. Ginkgetin

Ginkgetin, a biflavone, is isolated from Ginkgo biloba leaves. Ginkgetin exhibit anti-tumor, anti-inflammatory, neuroprotective, anti-fungal activities. Ginkgetin is also a potent inhibitor of Wnt signaling, with an IC50 of 5.92 μΜ.

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Ginkgetin Chemical Structure

Ginkgetin Chemical Structure

CAS No. : 481-46-9

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 150 In-stock
Solution
10 mM * 1 mL in DMSO USD 150 In-stock
Solid
1 mg USD 50 In-stock
5 mg USD 120 In-stock
10 mg USD 192 In-stock
25 mg USD 384 In-stock
50 mg USD 615 In-stock
100 mg USD 984 In-stock
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Customer Review

Based on 4 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Ginkgetin, a biflavone, is isolated from Ginkgo biloba leaves. Ginkgetin exhibit anti-tumor, anti-inflammatory, neuroprotective, anti-fungal activities. Ginkgetin is also a potent inhibitor of Wnt signaling, with an IC50 of 5.92 μΜ[1][2][3][4][5].

In Vitro

Ginkgetin (2.5-20 μM; 48 h) inhibits the growth of Daoy and D283 cell lines, and induces G2/M cell cycle arrest in Daoy cells[2].
? Ginkgetin (20-40 μM; 24 h) significantly activates the apoptosis of osteosarcoma cells in a concentration-dependent manner[3].
? Ginkgetin (10-20 μM; 3-24 h) down-regulated the expression of Wnt target genes without affecting the expression of β-catenin in medulloblastoma cells[2].
? Ginkgetin (1-10 μM; 24 or 48 h) significantly inhibits the VEGF-induced endothelial cell proliferation, migration, and wound recovery in a concentration-dependent manner[1].
? Ginkgetin (5-10 μM; 48 h) induces autophagy responsible for cell death in A549[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[2]

Cell Line: Daoy and D283 cell lines
Concentration: 2.5, 5, 10, 20 μM
Incubation Time: 48 hours
Result: Inhibited the cell growth, with IC50s of 14.65 and 15.81 μM for Daoy and D283 cells, respectively.

Apoptosis Analysis[3]

Cell Line: Osteosarcoma cells
Concentration: 20, 30, 40 μM
Incubation Time: 24 hours
Result: Markedly induced the apoptosis of osteosarcoma cells in a concentration-dependent manner.

Cell Cycle Analysis[2]

Cell Line: Daoy cells
Concentration: 2.5, 5, 10, 20 μM
Incubation Time: 24 hours
Result: Increased G2/M phase, compared with that of control, indicating a G2/M cell phase arrest.

Cell Cycle Analysis[2]

Cell Line: Daoy and D283 cell lines
Concentration: 10, 20 μM
Incubation Time: 3, 6, 12, 24 hours
Result: Attenuated the expression of Wnt target genes, Axin2, cyclin D1 and survivin at 20 μM for 24 h in Daoy cells.
Unaffected the level of total β-catenin and diminished the level of β-catenin phosphorylation in a time- and concentration-dependent manner.
In Vivo

Ginkgetin (25-100 mg/kg; i.p. 2 hours after the onset of ischemia) exerts anti-inflammatory effects on cerebral ischemia/reperfusion-induced injury in a rat model via the TLR4/NF-κB signaling pathway[4].
? Ginkgetin (30 mg/kg; intragastrically once per day for 42 d) suppresses tumor growth in A549 cells bearing nude mice[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Sprague-Dawley rats (200-220 g)[4]
Dosage: 25, 50, 100 mg/kg
Administration: I.p. 2 hours after the onset of ischemia
Result: Reduced the neurological deficit score.
Suppressed the expression of NF-κB, TLR4 and IκBαin ischemic penumbra cortex, and inhibited the degradation of IκBα.
Decreased the expressions of ICAM-1, COX-2, and iNOS.
Downregulated downstream inflammatory factor PGE2 and TNF-α expression.
Decreased IL-1β, IL-6, IL-8, and IL-10 protein expression.
Molecular Weight

566.51

Formula

C32H22O10

CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

OC1=CC(O)=C2C(OC(C3=CC=C(O)C=C3)=CC2=O)=C1C4=CC(C(OC5=CC(OC)=CC(O)=C56)=CC6=O)=CC=C4OC

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : ≥ 20.83 mg/mL (36.77 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7652 mL 8.8260 mL 17.6519 mL
5 mM 0.3530 mL 1.7652 mL 3.5304 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  50% PEG300    50% Saline

    Solubility: 2 mg/mL (3.53 mM); Suspended solution; Need ultrasonic and warming

  • Protocol 2

    Add each solvent one by one:  0.5% CMC-Na/saline water

    Solubility: 2 mg/mL (3.53 mM); Suspended solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.86%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.7652 mL 8.8260 mL 17.6519 mL 44.1298 mL
5 mM 0.3530 mL 1.7652 mL 3.5304 mL 8.8260 mL
10 mM 0.1765 mL 0.8826 mL 1.7652 mL 4.4130 mL
15 mM 0.1177 mL 0.5884 mL 1.1768 mL 2.9420 mL
20 mM 0.0883 mL 0.4413 mL 0.8826 mL 2.2065 mL
25 mM 0.0706 mL 0.3530 mL 0.7061 mL 1.7652 mL
30 mM 0.0588 mL 0.2942 mL 0.5884 mL 1.4710 mL
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Ginkgetin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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