Cat. No.: HY-N2360

Hinokiflavone

6 Cited Publications

Customer Review

Based on 6 publication(s) in Google Scholar

Hinokiflavone is a novel modulator of pre-mRNA splicing activity extracted from plants with anti-inflammatory, anti-tumor and antiviral activities. Hinokiflavone is also a potent inhibitor for matrix metalloproteinases (MMPs). Hinokiflavone attenuates the virulence of Methicillin (HY-121544)-resistant staphylococcus aureus by inhibiting caseinolytic protease P (ClpP) with an IC₅₀ value of 34.36 mg/mL. Hinokiflavone induces apoptosis via the reactive oxygen species-mitochondria-mediated apoptotic pathway and inhibits tumor cell migration and invasion. Hinokiflavone is a SUMO protease inhibitor against sentrin-specific protease 1 (SENP1) activity.

For research use only. We do not sell to patients.

Purity: 99.81%
CAS No.: 19202-36-9
Formula: C₃₀H₁₈O₁₀
Molecular Weight:538.46

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Hinokiflavone Chemical Structure

CAS No.: 19202-36-9

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (6)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

All MMP Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	IC₅₀	14.2 μM Compound: Hnk	Antiproliferative activity against human HCT-116 cells assessed as reduction in cell viability incubated for 24 hrs by Celltiter-Glo assay Antiproliferative activity against human HCT-116 cells assessed as reduction in cell viability incubated for 24 hrs by Celltiter-Glo assay	[PMID: 39185935]
HL-60	IC₅₀	11 μM Compound: Hnk	Antiproliferative activity against human HL-60 cells assessed as reduction in cell viability incubated for 24 hrs by Celltiter-Glo assay Antiproliferative activity against human HL-60 cells assessed as reduction in cell viability incubated for 24 hrs by Celltiter-Glo assay	[PMID: 39185935]
MCF7	IC₅₀	16.3 μM Compound: Hnk	Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 24 hrs by Celltiter-Glo assay Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 24 hrs by Celltiter-Glo assay	[PMID: 39185935]
Monocyte	IC₅₀	18 nM Compound: Hinokiflavone	Inhibition of procoagulant activity in monocyte from human blood assessed as counteraction of endotoxin-induced tissue factor expression after 18 hrs Inhibition of procoagulant activity in monocyte from human blood assessed as counteraction of endotoxin-induced tissue factor expression after 18 hrs	[PMID: 8882428]
Monocyte	IC₅₀	48 nM Compound: Hinokiflavone	Inhibition of procoagulant activity in monocyte from human blood assessed as counteraction of IL1-induced tissue factor expression after 18 hrs Inhibition of procoagulant activity in monocyte from human blood assessed as counteraction of IL1-induced tissue factor expression after 18 hrs	[PMID: 8882428]
OCI-AML2	IC₅₀	4.9 μM Compound: Hnk	Antiproliferative activity against human OCI-AML2 cells assessed as reduction in cell viability incubated for 24 hrs by Celltiter-Glo assay Antiproliferative activity against human OCI-AML2 cells assessed as reduction in cell viability incubated for 24 hrs by Celltiter-Glo assay	[PMID: 39185935]
PBMC	IC₅₀	9 μM Compound: 4	Cytotoxicity against uninfected PHA-stimulated human PBMC assessed as thymidine incorporation after 24 hrs by trypan blue exclusion method Cytotoxicity against uninfected PHA-stimulated human PBMC assessed as thymidine incorporation after 24 hrs by trypan blue exclusion method	[PMID: 9322359]

In Vitro

Hinokiflavone (500 μM, 90 min) inhibits splicing of the adenovirus and the HPV18E6 pre-mRNAs, showed a stronger effect on pre-mRNA splicing than Isoginkgetin (HY-N2117) in vitro^[1].
Hinokiflavone (0-30 μM, 24 h) induces either cell cycle arrest or eventual cell death and increases levels of SUMOylated proteins by inhibiting sentrin-specific protease 1 (SENP1) activity in HEK 293 cells^[1].
Hinokiflavone (0-40 μM, 24 h) suppresses colorectal tumor cell proliferation, cell migration and invasion ability but promotes cell Apoptosis via the mitochondria-mediated apoptotic pathway^[2].
Hinokiflavone (0-128 μg/mL, 24 h) reduces the expression of virulence factors in staphylococcus aureus by directly interacting with caseinolytic protease P (ClpP)^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR^[1]

Cell Line:

HEK293 cells
Concentration:

20-100 μM
Incubation Time:

24 h
Result:

Induced changes in alternative splicing patterns and modulated splicing in HEK293 cells.

Cell Cycle Analysis^[1]

Cell Line:

HEK293 cells, HeLa, NB4 cells
Concentration:

0-30 μM
Incubation Time:

24 h
Result:

Showed either cell cycle arrest or eventual cell death, dependent upon concentrations in HEK293 cells, HeLa, NB4 cells.

Apoptosis Analysis^[2]

Cell Line:

Colorectal tumor cells
Concentration:

0-40 μM
Incubation Time:

24 h
Result:

Reduced and shrinked tumor cells with nuclear fragmentation and condensed nuclei formation, decreased the expression of Bcl-2, but increased the expression of Bax and cleaved caspase-3.

Western Blot Analysis^[2]

Cell Line:

Colorectal tumor cells
Concentration:

0-40 μM
Incubation Time:

24 h
Result:

Inhibited the expression of MMP2 and MMP9 and increased the expression of TIMP2 in colorectal tumor cells.

Cell Cytotoxicity Assay^[3]

Cell Line:

HEK 293T cells
Concentration:

0-256 μg/mL
Incubation Time:

24 h
Result:

Did not affect HEK293T cells viability.

In Vivo

Hinokiflavone (25 or 50 mg/kg, i.p., daily for 24 days) has antitumor activity via suppressing tumor proliferation, metastasis and inducing Apoptosis in CT26 tumor-bearing mice^[2].
Hinokiflavone (100 mg/kg, s.c., every 12 h for 96 h) shows a protective effect on pneumonia infection by Improving survival rate in mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

CT26 tumor-bearing mice model^[2]
Dosage:

25 or 50 mg/kg
Administration:

i.p., daily for 24 days
Result:

Resulted in retarded tumor progression and reduction of tumor weight without affecting body weight of mice, suppressed tumor proliferation and invasion ability by staining nuclear Ki-67 and MMP9, induced the apoptosis by staining cleaved caspase-3.

Chemical Information

CAS No. 19202-36-9
Appearance Solid
Molecular Weight 538.46
Formula C₃₀H₁₈O₁₀
Color Light yellow to yellow
SMILES

O=C1C=C(C2=CC=C(O)C=C2)OC3=CC(O)=C(OC4=CC=C(C5=CC(C6=C(O)C=C(O)C=C6O5)=O)C=C4)C(O)=C13
Structure Classification
- Flavonoids
- Biflavones
- Phenols
- Polyphenols
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (6)

All Cell Proliferation/Viability AssayIFFlow CytometryIHCWB

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Sci Rep

Hinokiflavone is a novel CK2 inhibitor promoting apoptosis and synergizing with chemotherapeutic agents in cisplatin resistant bladder cancer cells. [Abstract]

2025 Jul 1;15(1):20922. PMID: 40594996

Hinokiflavone purchased from MedChemExpress. Usage Cited in: Sci Rep. 2025 Jul 1;15(1):20922. [Abstract]

Hinokiflavone (0.625, 1.25, 2.5, 5 μM) exhibits selective anti-proliferative activity in Cisplatin-resistant N/P(14) and J82R cells compared to normal uroepithelial SV-HUC-1 cells following 48 h of treatment.

Hinokiflavone purchased from MedChemExpress. Usage Cited in: Sci Rep. 2025 Jul 1;15(1):20922. [Abstract]

N/P(14) cells were treated with varying concentrations of Hinokiflavone (1.25, 2.5, 5, 10 μM) for 48 h, stained with Annexin V/7-AAD, and analyzed via flow cytometry.

Hinokiflavone purchased from MedChemExpress. Usage Cited in: Sci Rep. 2025 Jul 1;15(1):20922. [Abstract]

The effects of Hinokiflavone (HNK) (1.25, 2.5, 5, 10 μM) on the levels of BER-related proteins in N/P(14) cells were analyzed by western blotting.
J Photochem Photobiol B

Selaginella Tamariscina extract reduces UVA-induced skin photodamage via regulating apoptosis and autophagy by AKT phosphorylation. [Abstract]

2025 Dec 16:274:113343. PMID: 41421059
Sex Med

Hinokiflavone alleviates high-fat diet-induced erectile dysfunction via the EGFR/PI3K/Akt/eNOS signaling pathway. [Abstract]

2025 Aug 6;13(4):qfaf059. PMID: 40808867

Hinokiflavone purchased from MedChemExpress. Usage Cited in: Sex Med. 2025 Aug 6;13(4):qfaf059. [Abstract]

Representative images of EdU assays in RCCECs treated with PA (0.3 mM, 24 h) and Hinokiflavone (HINO) (5 μM, 24 h).

Hinokiflavone purchased from MedChemExpress. Usage Cited in: Sex Med. 2025 Aug 6;13(4):qfaf059. [Abstract]

Immunohistochemical staining and semi-qualification of Ki67 and SOD1 in the penile tissue of rats treated with Hinokiflavone (HINO) (2.5 mg/kg/day, p.o.).
Reprod Domest Anim

Depletion of TIAR impairs embryogenesis via inhibiting zygote genome transcribe. [Abstract]

2023 Oct;58(10):1456-1467. PMID: 37667420
SSRN

2025 Jan 10.
bioRxiv

2024 July 27.

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (92.86 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 2 mg/mL (3.71 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.8571 mL

9.2857 mL

18.5715 mL

5 mM

0.3714 mL

1.8571 mL

3.7143 mL

10 mM

0.1857 mL

0.9286 mL

1.8571 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 0.83 mg/mL (1.54 mM); Clear solution

This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 50% PEG300 50% Saline

Solubility: 10 mg/mL (18.57 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Pawellek A, et al. Characterisation of the biflavonoid hinokiflavone as a pre-mRNA splicing modulator that inhibits SENP. Elife. 2017 Sep 8;6. pii: e27402. [Content Brief]

[2]. Zhou J, et al. Antitumor activity in colorectal cancer induced by hinokiflavone. J Gastroenterol Hepatol. 2019 Sep;34(9):1571-1580. [Content Brief]

[3]. Kong X, et al. Hinokiflavone Attenuates the Virulence of Methicillin-Resistant Staphylococcus aureus by Targeting Caseinolytic Protease P. Antimicrob Agents Chemother. 2022 Aug 16;66(8):e0024022. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
Ethanol / DMSO	1 mM	1.8571 mL	9.2857 mL	18.5715 mL	46.4287 mL
DMSO	5 mM	0.3714 mL	1.8571 mL	3.7143 mL	9.2857 mL
	10 mM	0.1857 mL	0.9286 mL	1.8571 mL	4.6429 mL
	15 mM	0.1238 mL	0.6190 mL	1.2381 mL	3.0952 mL
	20 mM	0.0929 mL	0.4643 mL	0.9286 mL	2.3214 mL
	25 mM	0.0743 mL	0.3714 mL	0.7429 mL	1.8571 mL
	30 mM	0.0619 mL	0.3095 mL	0.6190 mL	1.5476 mL
	40 mM	0.0464 mL	0.2321 mL	0.4643 mL	1.1607 mL
	50 mM	0.0371 mL	0.1857 mL	0.3714 mL	0.9286 mL
	60 mM	0.0310 mL	0.1548 mL	0.3095 mL	0.7738 mL
	80 mM	0.0232 mL	0.1161 mL	0.2321 mL	0.5804 mL

Keywords

Hinokiflavone

6 Cited Publications

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Hinokiflavone

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Publications Citing Use of MedChemExpress (MCE) Hinokiflavone

All MMP Isoforms

Biological Activity

Chemical Information

Publications (6)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Hinokiflavone Related Classifications

Cat. No.: HY-N2360