Berberine chloride (Synonyms: Natural Yellow 18 chloride)

Name: Berberine chloride
Brand: MedChemExpress
SKU: HY-18258
Rating: 5.0 (80 reviews)

Cat. No.: HY-18258 Purity: 99.92%: Data Sheet
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Berberine chloride is an alkaloid that acts as an antibiotic. Berberine chloride induces reactive oxygen species (ROS) generation and inhibits DNA topoisomerase. Antineoplastic properties.

For research use only. We do not sell to patients.

CAS No. : 633-65-8

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	In-stock	Estimated Time of Arrival: December 31
25 g	In-stock	Estimated Time of Arrival: December 31
50 g	In-stock	Estimated Time of Arrival: December 31
100 g	In-stock	Estimated Time of Arrival: December 31
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Based on 80 publication(s) in Google Scholar

Other Forms of Berberine chloride:

80 Publications Citing Use of MCE Berberine chloride

In Vivo Efficacy Study

Bio/Physico-chemical Assay

RT-PCR

Others

Cell Proliferation/Viability Assay

: Berberine chloride purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jan 2;16(1):212. [Abstract]; A549 (1 ×10⁶) cells were subcutaneously inoculated into the right scruff of the female BALB/c nude mouse (n = 8/group). On day 22 after inoculation, mice were given Berberine (p.o.; 40 mg/kg; daily for 12 days) . The tumor volumes on indicated days after gavage were examined

: Berberine chloride purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jan 2;16(1):212. [Abstract]; H1299 cells were treated with or without Berberine (Ber;5 μM; 24 h). Cells were subjected to qPCR (n = 3 independent experiments) analyses.

: Berberine chloride purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2025 Sep;15(9):4789-4806.; The hydroxyproline assay kit was used to detect the secretion of fibroblast collagen in NMCFs by Berberine (BBC; 70 μM）. n=3-4. *P< 0.05, **P< 0.01 vs. CTL #P<0.05, ###P <0.001 vs. TGF-β1.

: Berberine chloride purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2025 Sep;15(9):4789-4806.; Immunofluorescence in determining the expression of P4HA1 after treated with Berberine (BBC; p.o.; 20 mg/kg for 7 days ) and Selonsertib (p.o.; 20 mg/kg for 7 days ) in the heart tissue of MI mice. n=3. *P < 0.05, **P < 0.01 vs. Sham, #P < 0.05, ##P < 0.01 vs. MI. Scale bar, 200 µm.

: Berberine chloride purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2025 Sep;15(9):4789-4806.; Echocardiographic detection of cardiac function changes in MI mice and treated with Berberine (BBC; p.o.; 20 mg/kg for 7 days ) and Selonsertib (p.o.; 20 mg/kg for 7 days ) . n=3-4. *P < 0.05 vs. Sham, #P < 0.05 vs. MI.

: Berberine chloride purchased from MedChemExpress. Usage Cited in: BMC Pharmacol Toxicol. 2023 May 11;24(1):29. [Abstract]; Berberine (BBR; 2.5, 5, 10 µM; 48 h) inhibits EGFR phosphorylation in a dose-dependent matter in A431cells.

: Berberine chloride purchased from MedChemExpress. Usage Cited in: BMC Pharmacol Toxicol. 2023 May 11;24(1):29. [Abstract]; Berberine (BBR; 2.5 µM; 10 days) significantly inhibits NCI-H441 colony formation.

: Berberine chloride purchased from MedChemExpress. Usage Cited in: BMC Pharmacol Toxicol. 2023 May 11;24(1):29. [Abstract]; Berberine (BBR; 0.625, 1.25, 2.5, 5, 10 µM; 48 h) inhibits the viability of A431cells in a dose-dependent matter.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

ROS^[1]
DNA topoisomerase^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
A10	GI₅₀	101.62 μM Compound: 2	Growth inhibition against rat A10 cells after 72 hrs by MTT assay Growth inhibition against rat A10 cells after 72 hrs by MTT assay	[PMID: 21401114]
A2780	IC₅₀	> 50 μM Compound: BBR; 1	Antiproliferative activity against human A2780 cells assessed as reduction in cell viability after 48 hrs by MTT assay Antiproliferative activity against human A2780 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 32996316]
A549	IC₅₀	6.27 μM Compound: 10	Cytotoxicity against human A549 cells by SRB assay Cytotoxicity against human A549 cells by SRB assay	[PMID: 20594848]
A549	IC₅₀	> 50 μM Compound: BBR	Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 38086195]
A549	IC₅₀	> 50 μM Compound: BBR; 1	Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 32996316]
BGC-823	IC₅₀	7.04 μM Compound: 1, Berberine	Cytotoxicity against human BGC823 cells after 48 hrs by MTT assay Cytotoxicity against human BGC823 cells after 48 hrs by MTT assay	10.1007/s00044-013-0796-9
Bel-7402	IC₅₀	16.28 μM Compound: 1, Berberine	Cytotoxicity against human Bel7402 cells after 48 hrs by MTT assay Cytotoxicity against human Bel7402 cells after 48 hrs by MTT assay	10.1007/s00044-013-0796-9
C6	IC₅₀	> 20 μM Compound: 1	Cytotoxicity against rat C6 cells assessed as inhibition of cell proliferation after 24 hrs by MTT assay Cytotoxicity against rat C6 cells assessed as inhibition of cell proliferation after 24 hrs by MTT assay	10.1039/C4MD00264D
CCRF S-180	IC₅₀	25 μM Compound: BBR	Antiproliferative activity against mouse CCRF S-180 cells assessed as inhibition of cell proliferation incubated for 48 hrs by MTT assay Antiproliferative activity against mouse CCRF S-180 cells assessed as inhibition of cell proliferation incubated for 48 hrs by MTT assay	[PMID: 33848698]
CCRF-CEM	CC₅₀	2.09 μM Compound: 1	Cytotoxicity against human CEM cells expressing green fluorescent protein after 48 hrs by MTT assay Cytotoxicity against human CEM cells expressing green fluorescent protein after 48 hrs by MTT assay	[PMID: 21295891]
CCRF-CEM	EC₅₀	0.13 μM Compound: 1	Antiviral activity against 0.05 MOI Human immunodeficiency virus 1 NL4.3 infected in human CEM cells expressing green fluorescent protein assessed as p24 antigen production measured 7 days post infection by ELISA Antiviral activity against 0.05 MOI Human immunodeficiency virus 1 NL4.3 infected in human CEM cells expressing green fluorescent protein assessed as p24 antigen production measured 7 days post infection by ELISA	[PMID: 21295891]
CHO-K1	IC₅₀	336 μM Compound: BBR	Cytotoxicity against CHO-K1 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay Cytotoxicity against CHO-K1 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay	[PMID: 32688201]
DU-145	IC₅₀	> 20 μM Compound: BBR	Antiproliferative activity against human DU145 cells assessed as cell growth inhibition by SRB assay Antiproliferative activity against human DU145 cells assessed as cell growth inhibition by SRB assay	[PMID: 31812467]
G-402	GI₅₀	11.87 μM Compound: 2	Growth inhibition against human G402 cells after 48 hrs by MTT assay Growth inhibition against human G402 cells after 48 hrs by MTT assay	[PMID: 21401114]
HCT-116	IC₅₀	25 μM Compound: BBR	Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell proliferation incubated for 48 hrs by MTT assay Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell proliferation incubated for 48 hrs by MTT assay	[PMID: 33848698]
HCT-116	IC₅₀	39.95 μM Compound: Berberine Chloride	Cytotoxicity against human HCT116 cells after 72 hrs by MTT assay Cytotoxicity against human HCT116 cells after 72 hrs by MTT assay	[PMID: 28359045]
HCT-116	IC₅₀	83.09 μM Compound: Ber	Inhibition of cell proliferation of human HCT116 cells incubated for 15 hrs by MTT assay Inhibition of cell proliferation of human HCT116 cells incubated for 15 hrs by MTT assay	[PMID: 32391701]
HCT-116	IC₅₀	> 10 μM Compound: BBR	Antiproliferative activity against human HCT116 cells assessed as cell growth inhibition by SRB assay Antiproliferative activity against human HCT116 cells assessed as cell growth inhibition by SRB assay	[PMID: 31812467]
HCT-15	IC₅₀	16.59 μM Compound: 10	Cytotoxicity against human HCT15 cells by SRB assay Cytotoxicity against human HCT15 cells by SRB assay	[PMID: 20594848]
HCT-8	IC₅₀	25 μM Compound: BBR	Antiproliferative activity against human HCT-8 cells assessed as inhibition of cell proliferation incubated for 48 hrs by MTT assay Antiproliferative activity against human HCT-8 cells assessed as inhibition of cell proliferation incubated for 48 hrs by MTT assay	[PMID: 33848698]
HL-60	IC₅₀	27.42 μM Compound: Berberine Chloride	Cytotoxicity against human HL60 cells after 72 hrs by MTT assay Cytotoxicity against human HL60 cells after 72 hrs by MTT assay	[PMID: 28359045]
HL-60	IC₅₀	5.83 μM Compound: 1, Berberine	Cytotoxicity against human HL60 cells after 48 hrs by MTT assay Cytotoxicity against human HL60 cells after 48 hrs by MTT assay	10.1007/s00044-013-0796-9
HT-29	IC₅₀	8.45 μM Compound: 1, Berberine	Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay	[PMID: 23182088]
HT-29	IC₅₀	> 10 μM Compound: BBR	Antiproliferative activity against human HT-29 cells assessed as cell growth inhibition by SRB assay Antiproliferative activity against human HT-29 cells assessed as cell growth inhibition by SRB assay	[PMID: 31812467]
HT-29	IC₅₀	> 20 μM Compound: 1, Berberine	Cytotoxicity against human HT-29 cells after 24 hrs by MTT assay Cytotoxicity against human HT-29 cells after 24 hrs by MTT assay	[PMID: 23182088]
HT-29	IC₅₀	> 50 μM Compound: BBR; 1	Antiproliferative activity against human HT29 cells assessed as reduction in cell viability after 48 hrs by MTT assay Antiproliferative activity against human HT29 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 32996316]
HeLa	IC₅₀	138.4 g/mL Compound: Berberine, I	Cytotoxicity against human HeLa cells after 24 hrs by MTT assay Cytotoxicity against human HeLa cells after 24 hrs by MTT assay	[PMID: 18295494]
HepG2	IC₅₀	11.22 μM Compound: 1, Berberine	Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay	[PMID: 23182088]
HepG2	IC₅₀	13.8 μM Compound: BER	Down regulation of PCSK9 mRNA expression in human HepG2 cells incubated for 24 hrs by SYBR green dye-based qRT-PCR analysis Down regulation of PCSK9 mRNA expression in human HepG2 cells incubated for 24 hrs by SYBR green dye-based qRT-PCR analysis	[PMID: 33567826]
HepG2	IC₅₀	146.2 g/mL Compound: Berberine, I	Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay	[PMID: 18295494]
HepG2	IC₅₀	49.01 μM Compound: Berberine Chloride	Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay	[PMID: 28359045]
HepG2	IC₅₀	53.91 μg/mL Compound: Berberine chloride	Cytotoxicity against human HepG2 cells after 12 hrs by CCK-8 assay Cytotoxicity against human HepG2 cells after 12 hrs by CCK-8 assay	[PMID: 31057739]
HepG2	IC₅₀	8.32 μM Compound: 1, Berberine	Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay	[PMID: 23182088]
HepG2	IC₅₀	> 50 μM Compound: BBR	Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 38086195]
HepG2	IC₅₀	> 50 μM Compound: BBR; 1	Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability after 48 hrs by MTT assay Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 32996316]
Hepatocyte	IC₅₀	26.5 μg/mL Compound: Berberine, I	Cytotoxicity against Sprague-Dawley rat primary hepatocytes after 24 hrs by MTT assay Cytotoxicity against Sprague-Dawley rat primary hepatocytes after 24 hrs by MTT assay	[PMID: 18295494]
Huh-7	CC₅₀	> 100 μM Compound: GNF-Pf-4545	NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7) NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7)	[PMID: 18579783]
KB	IC₅₀	6.03 μM Compound: 1, Berberine	Cytotoxicity against human KB cells after 48 hrs by MTT assay Cytotoxicity against human KB cells after 48 hrs by MTT assay	10.1007/s00044-013-0796-9
KB	IC₅₀	7.32 μM Compound: Berberine chloride	Cytotoxicity against human KB cells after 72 hrs Cytotoxicity against human KB cells after 72 hrs	[PMID: 11141105]
KM12C	IC₅₀	76.34 μM Compound: Ber	Inhibition of cell proliferation of human KM12C cells incubated for 15 hrs by MTT assay Inhibition of cell proliferation of human KM12C cells incubated for 15 hrs by MTT assay	[PMID: 32391701]
L02	IC₅₀	71.55 μM Compound: Berberine Chloride	Cytotoxicity against human LO2 cells after 72 hrs by MTT assay Cytotoxicity against human LO2 cells after 72 hrs by MTT assay	[PMID: 28359045]
L02	IC₅₀	> 50 μM Compound: BBR	Cytotoxicity against human HL7702 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human HL7702 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 38086195]
L02	IC₅₀	> 50 μM Compound: BBR; 1	Antiproliferative activity against human L02 cells assessed as reduction in cell viability after 48 hrs by MTT assay Antiproliferative activity against human L02 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 32996316]
LNCaP	IC₅₀	> 50 μM Compound: Berberine chloride	Antiproliferative activity against human LNCaP cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay Antiproliferative activity against human LNCaP cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay	[PMID: 38215590]
LS174T	IC₅₀	25 μM Compound: BBR	Antiproliferative activity against human LS174T cells assessed as inhibition of cell proliferation incubated for 48 hrs by MTT assay Antiproliferative activity against human LS174T cells assessed as inhibition of cell proliferation incubated for 48 hrs by MTT assay	[PMID: 33848698]
MCF-10A	IC₅₀	> 50 μM Compound: Berberine chloride	Antiproliferative activity against human MCF-10A cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay Antiproliferative activity against human MCF-10A cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay	[PMID: 38215590]
MCF7	IC₅₀	75 μM Compound: berberine	Inhibition of telomerase expressed in MCF7 cells by TRAP assay Inhibition of telomerase expressed in MCF7 cells by TRAP assay	[PMID: 17574421]
MCF7	IC₅₀	> 50 μM Compound: Berberine chloride	Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay	[PMID: 38215590]
MDA-MB-231	IC₅₀	20.6 μM Compound: BBR	Antiproliferative activity against human MDA-MB-231 cells assessed as cell growth inhibition by SRB assay Antiproliferative activity against human MDA-MB-231 cells assessed as cell growth inhibition by SRB assay	[PMID: 31812467]
MKN-28	IC₅₀	25 μM Compound: BBR	Antiproliferative activity against human MKN-28 cells assessed as inhibition of cell proliferation incubated for 48 hrs by MTT assay Antiproliferative activity against human MKN-28 cells assessed as inhibition of cell proliferation incubated for 48 hrs by MTT assay	[PMID: 33848698]
MRC5	CC₅₀	74 μM Compound: 1	Cytotoxicity against MRC5 cells Cytotoxicity against MRC5 cells	[PMID: 17239594]
MRC5	IC₅₀	0.68 μM Compound: 1	Antiviral activity against HCMV in MRC5 cells by plaque reduction assay Antiviral activity against HCMV in MRC5 cells by plaque reduction assay	[PMID: 17239594]
NCI-H446	IC₅₀	> 50 μM Compound: BBR	Antiproliferative activity against human NCI-H446 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Antiproliferative activity against human NCI-H446 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 38086195]
PANC-1	IC₅₀	> 50 μM Compound: BBR; 1	Antiproliferative activity against human PANC1 cells assessed as reduction in cell viability after 48 hrs by MTT assay Antiproliferative activity against human PANC1 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 32996316]
PANC-1	IC₅₀	> 50 μM Compound: Berberine chloride	Antiproliferative activity against human PANC-1 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay Antiproliferative activity against human PANC-1 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay	[PMID: 38215590]
PC-12	CC₅₀	> 150 μM Compound: 1	Cytotoxicity against rat PC12 cells assessed as reduction in cell viability incubated for 24 hrs by WST-8 assay Cytotoxicity against rat PC12 cells assessed as reduction in cell viability incubated for 24 hrs by WST-8 assay	[PMID: 33611188]
PC-3	IC₅₀	14.39 μM Compound: BBR	Antiproliferative activity against human PC3 cells assessed as cell growth inhibition by SRB assay Antiproliferative activity against human PC3 cells assessed as cell growth inhibition by SRB assay	[PMID: 31812467]
PC-3	IC₅₀	31.72 μM Compound: BBR; 1	Antiproliferative activity against human PC-3 cells assessed as reduction in cell viability after 48 hrs by MTT assay Antiproliferative activity against human PC-3 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 32996316]
SGC-7901	IC₅₀	25 μM Compound: BBR	Antiproliferative activity against human SGC-7901 cells assessed as inhibition of cell proliferation incubated for 48 hrs by MTT assay Antiproliferative activity against human SGC-7901 cells assessed as inhibition of cell proliferation incubated for 48 hrs by MTT assay	[PMID: 33848698]
SK-MEL-2	IC₅₀	13.76 μM Compound: 10	Cytotoxicity against human SK-MEL-2 cells by SRB assay Cytotoxicity against human SK-MEL-2 cells by SRB assay	[PMID: 20594848]
SK-OV-3	IC₅₀	16.44 μM Compound: 10	Cytotoxicity against human SKOV3 cells by SRB assay Cytotoxicity against human SKOV3 cells by SRB assay	[PMID: 20594848]
SMMC-7721	IC₅₀	25.94 μM Compound: Berberine Chloride	Cytotoxicity against human SMMC7721 cells after 72 hrs by MTT assay Cytotoxicity against human SMMC7721 cells after 72 hrs by MTT assay	[PMID: 28359045]
SW-620	IC₅₀	126.2 μM Compound: Ber	Inhibition of cell proliferation of human SW620 cells incubated for 15 hrs by MTT assay Inhibition of cell proliferation of human SW620 cells incubated for 15 hrs by MTT assay	[PMID: 32391701]
SW-620	IC₅₀	25 μM Compound: BBR	Antiproliferative activity against human SW620 cells assessed as inhibition of cell proliferation incubated for 48 hrs by MTT assay Antiproliferative activity against human SW620 cells assessed as inhibition of cell proliferation incubated for 48 hrs by MTT assay	[PMID: 33848698]
THP-1	IC₅₀	5.1 μM Compound: BBR	Inhibition of LPS + nigericin-induced IL-1beta secretion in PMA-differentiated human THP-1 cells incubated for 1 to 24 hrs by Immunoblot analysis Inhibition of LPS + nigericin-induced IL-1beta secretion in PMA-differentiated human THP-1 cells incubated for 1 to 24 hrs by Immunoblot analysis	[PMID: 33440945]
U-87MG ATCC	IC₅₀	> 20 μM Compound: 1	Cytotoxicity against human U87 cells assessed as inhibition of cell proliferation after 24 hrs by MTT assay Cytotoxicity against human U87 cells assessed as inhibition of cell proliferation after 24 hrs by MTT assay	10.1039/C4MD00264D
U-937	IC₅₀	35 μM Compound: Berberine chloride	Cytotoxicity against human U937 cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay Cytotoxicity against human U937 cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay	10.1039/C0MD00241K
VSMC	GI₅₀	95.1 μM Compound: 2	Antiproliferative activity against rat VSMC assessed as cell growth inhibition Antiproliferative activity against rat VSMC assessed as cell growth inhibition	[PMID: 21401114]
VSMC	GI₅₀	95.14 μM Compound: 2	Growth inhibition against rat VSMC after 72 hrs by MTT assay Growth inhibition against rat VSMC after 72 hrs by MTT assay	[PMID: 21401114]

In Vitro

Berberine (1.25-160 μM; 72 hours) has potential inhibitory effects on the proliferation of four colorectal carcinoma cell lines LoVo, HCT116, SW480, and HT-29^[1].
Berberine (1.25-160 μM; 24-72 hours) induces a time- and dose-dependent inhibition of LoVo cell growth^[1].
LoVo cells are exposure to Berberine (10-80 μM) for 24 h. Cell cycle analysis of 40 μM Berberine-treated LoVo cells by flow cytometry shows accumulation of cells in the G2/M phase^[1].
Berberine (10-80 μM) suppresses cyclin B1, cdc2 and cdc25c protein expression after 24 h, especially at the dose of 80.0 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	Four colorectal carcinoma cell lines LoVo, HCT116, SW480, and HT-29
Concentration:	1.25, 2.5, 5, 10, 20, 40, 80, and 160 μM
Incubation Time:	72 hours
Result:	Inhibited the proliferation of four cell lines. The IC₅₀ ranged from 40.8±4.1 μM (LoVo) to 98.6±2.9 μM (HCT116).

Cell Proliferation Assay^[1]

Cell Line:	Colorectal carcinoma cell lines LoVo
Concentration:	1.25, 2.5, 5, 10, 20, 40, 80, and 160 μM
Incubation Time:	24, 48, 72 hours
Result:	Induced a time- and dose-dependent inhibition of cell growth. By 72 h, 160.0 μM induced 71.1±1.9 % growth inhibitions in LoVo cells.

Cell Cycle Analysis^[1]

Cell Line:	LoVo cells
Concentration:	0, 10, 20, 40, or 80 μM
Incubation Time:	24 hours
Result:	Exposure to 40.0 μM induced G2/M-phase cell cycle arrest, an increase in the G2/M-phase population and a progressive decline in the G1 population.

Western Blot Analysis^[1]

Cell Line:	LoVo cells
Concentration:	10, 20, 40, or 80 μM
Incubation Time:	24 hours
Result:	Suppressed cyclin B1, cdc2 and cdc25c protein expression.

In Vivo

Berberine (10, 30, or 50 mg/kg/day; gastrointestinal gavage; for 10 consecutive days) inhibits the growth of human colorectal adenocarcinoma in vivo. Berberine at doses of 30 and 50 mg/kg/day taken by gastrointestinal gavage shows inhibitory rates of 33.1% and 45.3% on the human colorectal adenocarcinoma xenograft growth in nude mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	5-week-old BALB/c nu/nu mice with human colorectal adenocarcinoma LoVo xenografts^[1]
Dosage:	10, 30, or 50 mg/kg/day
Administration:	Gastrointestinal gavage; for 10 consecutive days
Result:	Showed inhibitory rates of 33.1 % and 45.3 % at doses of 30 and 50 mg/kg/day.

Molecular Weight

371.81

Formula

C₂₀H₁₈ClNO₄

CAS No.

633-65-8

Appearance

Solid

Color

Light yellow to yellow

SMILES

COC1=C(OC)C2=C[N+]3=C(C(C(CC3)=C4)=CC5=C4OCO5)C=C2C=C1.[Cl-]

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 8.33 mg/mL (22.40 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.6895 mL	13.4477 mL	26.8955 mL
5 mM	0.5379 mL	2.6895 mL	5.3791 mL
10 mM	0.2690 mL	1.3448 mL	2.6895 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (3.36 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (3.36 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 0.5% CMC-Na/saline water
Solubility: 11 mg/mL (29.59 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.92%

Select Batch:

Data Sheet (281 KB) SDS (396 KB)

COA (252 KB) RP-HPLC (218 KB)

Handling Instructions (2659 KB)

References

[1]. Cai Y, et al. Berberine inhibits the growth of human colorectal adenocarcinoma in vitro and in vivo. J Nat Med. 2014 Jan;68(1):53-62. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.6895 mL	13.4477 mL	26.8955 mL	67.2386 mL
	5 mM	0.5379 mL	2.6895 mL	5.3791 mL	13.4477 mL
	10 mM	0.2690 mL	1.3448 mL	2.6895 mL	6.7239 mL
	15 mM	0.1793 mL	0.8965 mL	1.7930 mL	4.4826 mL
	20 mM	0.1345 mL	0.6724 mL	1.3448 mL	3.3619 mL

Berberine chloride Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Berberine633-65-8Natural Yellow 18Natural Yellow18Natural Yellow-18TopoisomeraseAutophagyBacterialReactive Oxygen Species (ROS)AntibioticEndogenous MetaboliteParasiteInhibitorinhibitorinhibit

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Berberine chloride (Synonyms: Natural Yellow 18 chloride)

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