1. Cell Cycle/DNA Damage
    Autophagy
    Anti-infection
    NF-κB
    Metabolic Enzyme/Protease
    Immunology/Inflammation
  2. Topoisomerase
    Autophagy
    Bacterial
    Reactive Oxygen Species
  3. Berberine sulfate

Berberine sulfate (Synonyms: Natural Yellow 18 sulfate)

Cat. No.: HY-N0716B
Handling Instructions

Berberine sulfate is an alkaloid isolated from the Chinese herbal medicine Huanglian, as an antibiotic. Berberine sulfate induces reactive oxygen species (ROS) generation and inhibits DNA topoisomerase. Berberine sulfate has antineoplastic properties.

For research use only. We do not sell to patients.

Berberine sulfate Chemical Structure

Berberine sulfate Chemical Structure

CAS No. : 633-66-9

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Estimated Time of Arrival: December 31
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Based on 3 publication(s) in Google Scholar

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Description

Berberine sulfate is an alkaloid isolated from the Chinese herbal medicine Huanglian, as an antibiotic. Berberine sulfate induces reactive oxygen species (ROS) generation and inhibits DNA topoisomerase. Berberine sulfate has antineoplastic properties[1].

IC50 & Target

ROS[1]
DNA topoisomerase[1]

In Vitro

Berberine sulfate (1.25-160 μM; 72 hours) has potential inhibitory effects on the proliferation of four colorectal carcinoma cell lines LoVo, HCT116, SW480, and HT-29[1].
Berberine sulfate (1.25-160 μM; 24-72 hours) induces a time- and dose-dependent inhibition of LoVo cell growth[1].
LoVo cells are exposure to Berberine sulfate (10-80 μM) for 24 h. Cell cycle analysis of 40 μM Berberine-treated LoVo cells by flow cytometry shows accumulation of cells in the G2/M phase[1].
Berberine sulfate (10-80 μM) suppresses cyclin B1, cdc2 and cdc25c protein expression after 24 h, especially at the dose of 80.0 μM[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay[1]

Cell Line: Four colorectal carcinoma cell lines LoVo, HCT116, SW480, and HT-29
Concentration: 1.25, 2.5, 5, 10, 20, 40, 80, and 160 μM
Incubation Time: 72 hours
Result: Inhibited the proliferation of four cell lines. The IC50 ranged from 40.8±4.1 μM (LoVo) to 98.6±2.9 μM (HCT116).

Cell Proliferation Assay[1]

Cell Line: Colorectal carcinoma cell lines LoVo
Concentration: 1.25, 2.5, 5, 10, 20, 40, 80, and 160 μM
Incubation Time: 24, 48, 72 hours
Result: Induced a time- and dose-dependent inhibition of cell growth. By 72 h, 160.0 μM induced 71.1±1.9 % growth inhibitions in LoVo cells.

Cell Cycle Analysis[1]

Cell Line: LoVo cells
Concentration: 0, 10, 20, 40, or 80 μM
Incubation Time: 24 hours
Result: Exposure to 40.0 μM induced G2/M-phase cell cycle arrest, an increase in the G2/M-phase population and a progressive decline in the G1 population.

Western Blot Analysis[1]

Cell Line: LoVo cells
Concentration: 10, 20, 40, or 80 μM
Incubation Time: 24 hours
Result: Suppressed cyclin B1, cdc2 and cdc25c protein expression.
In Vivo

Berberine sulfate (10, 30, or 50 mg/kg/day; gastrointestinal gavage; for 10 consecutive days) inhibits the growth of human colorectal adenocarcinoma in vivo. Berberine at doses of 30 and 50 mg/kg/day taken by gastrointestinal gavage shows inhibitory rates of 33.1% and 45.3% on the human colorectal adenocarcinoma xenograft growth in nude mice[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 5-week-old BALB/c nu/nu mice with human colorectal adenocarcinoma LoVo xenografts[1]
Dosage: 10, 30, or 50 mg/kg/day
Administration: Gastrointestinal gavage; for 10 consecutive days
Result: Showed inhibitory rates of 33.1% and 45.3% at doses of 30 and 50 mg/kg/day.
Molecular Weight

433.43

Formula

C20H19NO8S

CAS No.
SMILES

COC1=C(OC)C2=C[N+]3=C(C(C(CC3)=C4)=CC5=C4OCO5)C=C2C=C1.O=S(O)([O-])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 5 mg/mL (11.54 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3072 mL 11.5359 mL 23.0718 mL
5 mM 0.4614 mL 2.3072 mL 4.6144 mL
10 mM 0.2307 mL 1.1536 mL 2.3072 mL
*Please refer to the solubility information to select the appropriate solvent.
References
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Berberine sulfate
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