2. Cell Cycle/DNA Damage Autophagy Anti-infection Immunology/Inflammation NF-κB Metabolic Enzyme/Protease
  3. Topoisomerase Autophagy Bacterial Reactive Oxygen Species Parasite
  4. Berberine sulfate

Berberine sulfate  (Synonyms: Natural Yellow 18 sulfate)

Cat. No.: HY-N0716B Purity: ≥98.0%
COA Handling Instructions

Molarity Calculator

Berberine sulfate is an alkaloid isolated from the Chinese herbal medicine Huanglian, as an antibiotic. Berberine sulfate induces reactive oxygen species (ROS) generation and inhibits DNA topoisomerase. Berberine sulfate has antineoplastic properties. The sulfate form improves bioavailability.

For research use only. We do not sell to patients.

Berberine sulfate Chemical Structure

Berberine sulfate Chemical Structure

CAS No. : 633-66-9

Size Price Stock Quantity
Free Sample (0.1 - 0.5 mg)   Apply Now  
25 mg USD 50 In-stock
50 mg USD 65 In-stock
100 mg USD 85 In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 35 publication(s) in Google Scholar

Other Forms of Berberine sulfate:

Berberine sulfate Related Antibodies

Top Publications Citing Use of Products

32 Publications Citing Use of MCE Berberine sulfate

WB
Proliferation Assay
Cell Viability Assay

    Berberine sulfate purchased from MedChemExpress. Usage Cited in: BMC Pharmacol Toxicol. 2023 May 11;24(1):29.  [Abstract]

    Berberine (BBR; 0.625, 1.25, 2.5, 5, 10 µM; 48 h) inhibits the viability of A431cells in a dose-dependent matter.

    Berberine sulfate purchased from MedChemExpress. Usage Cited in: BMC Pharmacol Toxicol. 2023 May 11;24(1):29.  [Abstract]

    Berberine (BBR; 2.5, 5, 10 µM; 48 h) inhibits EGFR phosphorylation in a dose-dependent matter in A431cells.

    Berberine sulfate purchased from MedChemExpress. Usage Cited in: BMC Pharmacol Toxicol. 2023 May 11;24(1):29.  [Abstract]

    Berberine (BBR; 2.5 µM; 10 days) significantly inhibits NCI-H441 colony formation.

    View All Topoisomerase Isoform Specific Products:

    View All Isoforms

    View All Parasite Isoform Specific Products:

    View All Isoforms

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Berberine sulfate is an alkaloid isolated from the Chinese herbal medicine Huanglian, as an antibiotic. Berberine sulfate induces reactive oxygen species (ROS) generation and inhibits DNA topoisomerase. Berberine sulfate has antineoplastic properties. The sulfate form improves bioavailability[1][2].

    IC50 & Target

    ROS[1]
    DNA topoisomerase[1]
    In Vitro

    Berberine sulfate (1.25-160 μM; 72 hours) has potential inhibitory effects on the proliferation of four colorectal carcinoma cell lines LoVo, HCT116, SW480, and HT-29[1].
    ? Berberine sulfate (1.25-160 μM; 24-72 hours) induces a time- and dose-dependent inhibition of LoVo cell growth[1].
    ? LoVo cells are exposure to Berberine sulfate (10-80 μM) for 24 h. Cell cycle analysis of 40 μM Berberine-treated LoVo cells by flow cytometry shows accumulation of cells in the G2/M phase[1].
    ? Berberine sulfate (10-80 μM) suppresses cyclin B1, cdc2 and cdc25c protein expression after 24 h, especially at the dose of 80.0 μM[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Berberine sulfate Related Antibodies

    Cell Proliferation Assay[1]

    Cell Line: Four colorectal carcinoma cell lines LoVo, HCT116, SW480, and HT-29
    Concentration: 1.25, 2.5, 5, 10, 20, 40, 80, and 160 μM
    Incubation Time: 72 hours
    Result: Inhibited the proliferation of four cell lines. The IC50 ranged from 40.8±4.1 μM (LoVo) to 98.6±2.9 μM (HCT116).

    Cell Proliferation Assay[1]

    Cell Line: Colorectal carcinoma cell lines LoVo
    Concentration: 1.25, 2.5, 5, 10, 20, 40, 80, and 160 μM
    Incubation Time: 24, 48, 72 hours
    Result: Induced a time- and dose-dependent inhibition of cell growth. By 72 h, 160.0 μM induced 71.1±1.9 % growth inhibitions in LoVo cells.

    Cell Cycle Analysis[1]

    Cell Line: LoVo cells
    Concentration: 0, 10, 20, 40, or 80 μM
    Incubation Time: 24 hours
    Result: Exposure to 40.0 μM induced G2/M-phase cell cycle arrest, an increase in the G2/M-phase population and a progressive decline in the G1 population.

    Western Blot Analysis[1]

    Cell Line: LoVo cells
    Concentration: 10, 20, 40, or 80 μM
    Incubation Time: 24 hours
    Result: Suppressed cyclin B1, cdc2 and cdc25c protein expression.
    In Vivo

    Berberine sulfate (10, 30, or 50 mg/kg/day; gastrointestinal gavage; for 10 consecutive days) inhibits the growth of human colorectal adenocarcinoma in vivo. Berberine at doses of 30 and 50 mg/kg/day taken by gastrointestinal gavage shows inhibitory rates of 33.1% and 45.3% on the human colorectal adenocarcinoma xenograft growth in nude mice[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: 5-week-old BALB/c nu/nu mice with human colorectal adenocarcinoma LoVo xenografts[1]
    Dosage: 10, 30, or 50 mg/kg/day
    Administration: Gastrointestinal gavage; for 10 consecutive days
    Result: Showed inhibitory rates of 33.1% and 45.3% at doses of 30 and 50 mg/kg/day.
    Clinical Trial
    Molecular Weight

    433.43

    Formula

    C20H19NO8S
    CAS No.
    Appearance

    Solid

    Color

    White to yellow

    SMILES

    COC1=C(OC)C2=C[N+]3=C(C(C(CC3)=C4)=CC5=C4OCO5)C=C2C=C1.O=S(O)([O-])=O
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    H2O : 50 mg/mL (115.36 mM; Need ultrasonic)

    DMSO : 0.5 mg/mL (1.15 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.3072 mL 11.5359 mL 23.0718 mL
    5 mM 0.4614 mL 2.3072 mL 4.6144 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: ≥98.0%

    COA (256 KB) HNMR (119 KB) LCMS (240 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 2.3072 mL 11.5359 mL 23.0718 mL 57.6794 mL
    H2O 5 mM 0.4614 mL 2.3072 mL 4.6144 mL 11.5359 mL
    10 mM 0.2307 mL 1.1536 mL 2.3072 mL 5.7679 mL
    15 mM 0.1538 mL 0.7691 mL 1.5381 mL 3.8453 mL
    20 mM 0.1154 mL 0.5768 mL 1.1536 mL 2.8840 mL
    25 mM 0.0923 mL 0.4614 mL 0.9229 mL 2.3072 mL
    30 mM 0.0769 mL 0.3845 mL 0.7691 mL 1.9226 mL
    40 mM 0.0577 mL 0.2884 mL 0.5768 mL 1.4420 mL
    50 mM 0.0461 mL 0.2307 mL 0.4614 mL 1.1536 mL
    60 mM 0.0385 mL 0.1923 mL 0.3845 mL 0.9613 mL
    80 mM 0.0288 mL 0.1442 mL 0.2884 mL 0.7210 mL
    100 mM 0.0231 mL 0.1154 mL 0.2307 mL 0.5768 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Berberine sulfate Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Berberine633-66-9Natural Yellow 18Natural Yellow18Natural Yellow-18TopoisomeraseAutophagyBacterialReactive Oxygen SpeciesParasiteInhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Salutation

    Applicant Name *

    		 

    Email Address *

    Phone Number *

    		 

    Organization Name *

    Department *

    		 

    Requested quantity *

    Country or Region *

    		      

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    Berberine sulfate
    Cat. No.:
    HY-N0716B
    Quantity:
    MCE Japan Authorized Agent:

    Customer Review

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Lot #

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Country or Region *

    Remarks

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.