Cat. No.: HY-N0716A

Berberine hemisulfate

(Synonyms: Natural Yellow 18 hemisulfate)

53 Cited Publications

Customer Review

Based on 53 publication(s) in Google Scholar

Berberine hemisulfate is the hemisulfate form of Berberine (HY-N0716). Berberine hemisulfate is an alkaloid isolated from the Chinese herbal medicine Huanglian. Berberine hemisulfate exhibits anti-inflammatory, antibiobic, antitumor, cardiovascular protective and neuroprotective activity.

For research use only. We do not sell to patients.

Purity: 99.63%
CAS No.: 316-41-6
Formula: C₂₀H₁₈NO₄.1/2O₄S
Molecular Weight:384.40

Storage:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Berberine hemisulfate Chemical Structure

CAS No.: 316-41-6

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Top Publications Citing

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
Publications (53)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All Antibiotic Isoforms :

All Topoisomerase Isoforms :

All PI3K Isoforms :

All Akt Isoforms :

All Caspase Isoforms :

All JNK Isoforms :

All AP-1 Isoforms :

All NF-κB Isoforms :

In Vitro

Berberine hemisulfate exhibits antimicrobial acitivity through inhibition of cell division protein FtsZ, or through DNA/RNA binding and deal thus DNA/RNAdamege^[3].
Berberine hemisulfate exhibts anti-inflammatory activity by inhibiting TNF-α and the activation of its downstream pathway AP-1 and NF-kB^[4].
Berberine hemisulfate exhibits neuroprotective efficacy by inhibiting the reactive oxygen species (ROS) production and caspase activation, and activatiing the PI3K/Akt signaling pathway, and heme oxygenase-1 (HO-1) expression^[5].
Berberine hemisulfate attenuates metabolic diseases through regulations of the lipids composition and inhibition of insulin resistance^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 316-41-6
Appearance Solid
Molecular Weight 384.40
Formula C₂₀H₁₈NO₄.1/2O₄S
Color Light yellow to yellow
SMILES

O=S([O-])([O-])=O.COC1=C(OC)C2=C[N+]3=C(C(C(CC3)=C4)=CC5=C4OCO5)C=C2C=C1.[0.5]
Synonyms

Natural Yellow 18 hemisulfate
Structure Classification
- Alkaloids
- Isoquinoline Alkaloids
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (53)

All WBCell Proliferation/Viability Assay

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Journal Impact Factor

Cell Stem Cell

Vagal pathway activation links chronic stress to decline in intestinal stem cell function. [Abstract]

2025 Mar 20:S1934-5909(25)00084-0. PMID: 40120585
J Hazard Mater

Ammonia-induced exosomal miRNA disrupts autophagy and promotes lung injury: Therapeutic potential of natural compounds. [Abstract]

2026 Mar 15:506:141417. PMID: 41775203
NPJ Biofilms Microbiomes

Berberine chloride hydrate impairs Streptococcus mutans biofilm formation via inhibiting sortase A activity. [Abstract]

2025 Jul 1;11(1):120. PMID: 40595693
Acta Pharmacol Sin

Tetrahydroberberrubine retards heart aging in mice by promoting PHB2-mediated mitophagy. [Abstract]

2023 Feb;44(2):332-344. PMID: 35948750
Phytomedicine

Huang-Lian-Jie-Du decoction enhances Capecitabine-Oxaliplatin efficacy via Akkermansia muciniphila and CD8+ T cells in colorectal cancer. [Abstract]

2025 Sep 4:148:157233. PMID: 40972261
Phytomedicine

Swiprosin-1 participates in the berberine-regulated AMPK/MLCK pathway to attenuate colitis-induced tight junction damage. [Abstract]

2024 Dec:135:156111. PMID: 39369569
Phytomedicine

Modified Hu-lu-ba-wan protects diabetic glomerular podocytes via promoting PKM2-mediated mitochondrial dynamic homeostasis. [Abstract]

2024 Jan:123:155247. PMID: 38128393
Free Radic Biol Med

Redox switch C674 in SERCA2 triggers Ca2+-calcineurin-MCU-Drp1 cascade and pulmonary vascular remodeling. [Abstract]

2025 Dec 16:244:380-394. PMID: 41412527
NPJ Sci Food

Network pharmacology study on the mechanism of berberine in Alzheimer's disease model. [Abstract]

2025 Feb 4;9(1):16. PMID: 39900946
Biomed Pharmacother

Berberine exerts antidepressant effects in vivo and in vitro through the PI3K/AKT/CREB/BDNF signaling pathway. [Abstract]

2024 Jan:170:116012. PMID: 38113631
Int J Nanomedicine

Combined Therapy of Experimental Autoimmune Uveitis by a Dual-Drug Nanocomposite Formulation with Berberine and Dexamethasone. [Abstract]

2023 Jul 31:18:4347-4363. PMID: 37545873
Mol Med

Berberine promotes primordial follicle activation and increases ovulated oocyte quantity in aged mice. [Abstract]

2024 Dec 20;30(1):251. PMID: 39707173
Phytother Res

A Novel Mechanism of Berberine Targeting EIF2AK2 Dimerization Attenuates Methylglyoxal-Induced Endothelial Senescence and Apoptosis. [Abstract]

2026 Mar;40(3):887-906. PMID: 41520368
JCI Insight

Age-related decline in hippocampal tyrosine phosphatase PTPRO is a mechanistic factor in chemotherapy-related cognitive impairment. [Abstract]

2023 Jul 24;8(14):e166306. PMID: 37485875
J Ethnopharmacol

Jiao-tai-wan and its effective component-berberine improve diabetes and depressive disorder through the cAMP/PKA/CREB signaling pathway. [Abstract]

2024 Apr 24:324:117829. PMID: 38296172
J Ethnopharmacol

Exploring the pathogenesis of type 2 diabetes mellitus intestinal damp-heat syndrome and the therapeutic effect of Gegen Qinlian Decoction from the perspective of exosomal miRNA. [Abstract]

2022 Mar 1:285:114786. PMID: 34763043
Pharm Biol

Berberine decreases S100B generation to regulate gut vascular barrier permeability in mice with burn injury. [Abstract]

2024 Dec;62(1):53-61. PMID: 38108311
Int Immunopharmacol

Berberine alleviates neuroinflammation by downregulating NFκB/LCN2 pathway in sepsis-associated encephalopathy: network pharmacology, bioinformatics, and experimental validation. [Abstract]

2024 May 30:133:112036. PMID: 38640713
Hepatol Commun

Oxyberberine sensitizes liver cancer cells to sorafenib via inhibiting NOTCH1-USP7-c-Myc pathway. [Abstract]

2024 Mar 29;8(4):e0405. PMID: 38573832
J Cell Mol Med

Berberine Sensitises Breast Cancer Cells to Radiation via the Attenuation of DNA Ligase III. [Abstract]

2025 Sep;29(17):e70836. PMID: 40916080
J Inflamm Res

Berberine Inhibits Acne-Related Lipid Secretion and Inflammation by Regulating the hsa-miR-3150a-3p/TP53 Pathway. [Abstract]

2025 Aug 21:18:11451-11461. PMID: 40860941
Sci Rep

Ursolic and oleanolic acids suppress MNNG induced malignant transformation of human gastric mucosal epithelium by regulating the PI3 K/AKT pathway. [Abstract]

2025 Jul 1;15(1):21623. PMID: 40594240
Fish Shellfish Immunol

Berberine inhibits SGIV replication by suppressing inflammatory response and oxidative stress. [Abstract]

2024 Jun:149:109522. PMID: 38548190
Sci Rep

Enhanced CHOLESTEROL biosynthesis promotes breast cancer metastasis via modulating CCDC25 expression and neutrophil extracellular traps formation. [Abstract]

2022 Oct 17;12(1):17350. PMID: 36253427
Aquaculture

15 May 2022, 738055.
Microbiol Spectr

The inhibitory effect of berberine chloride hydrate on Streptococcus mutans biofilm formation at different pH values. [Abstract]

2023 Sep 25;11(5):e0217023. PMID: 37747238
Microb Pathog

Berberine inhibits Mycoplasma synoviae infection by suppressing PIK3CA-dependent inflammatory and apoptotic responses in avian macrophages. [Abstract]

2025 Dec:209:108114. PMID: 41115537
Exp Cell Res

Berberine increases the killing effect of pirarubicin on HCC cells by inhibiting ATG4B-autophagy pathway. [Abstract]

2024 May 13:114094. PMID: 38750718
Food Chem Toxicol

Berberine attenuates sunitinib-induced cardiac dysfunction by normalizing calcium regulation disorder via SGK1 activation. [Abstract]

2023 May:175:113743. PMID: 36972840
Mol Med Rep

Triptolide promotes ferroptosis by suppressing Nrf2 to overcome leukemia cell resistance to doxorubicin. [Abstract]

2023 Jan;27(1):17. PMID: 36453238
Exp Cell Res

Network-based analysis with primary cells reveals drug response landscape of acute myeloid leukemia. [Abstract]

2020 Aug 1;393(1):112054. PMID: 32376287
Toxicol Appl Pharmacol

Berberine alleviates acute embolism-induced lung inflammation and injury by suppressing miR-22-5p expression and modulating the TLR4/NF-κB signaling pathway. [Abstract]

2025 Sep 13:505:117563. PMID: 40953642
Appl Biochem Biotechnol

Berberine Protects Against Dihydrotestosterone-Induced Human Ovarian Granulosa Cell Injury and Ferroptosis by Regulating the Circ_0097636/MiR-186-5p/SIRT3 Pathway. [Abstract]

2024 Aug;196(8):5265-5282. PMID: 38153651
J Pharm Pharmacol

Berberine suppresses the migration and invasion of colon cancer cells by inhibition of lipogenesis through modulation of promyelocytic leukemia zinc finger-mediated sterol-regulatory element binding proteins cleavage-activating protein ubiquitination. [Abstract]

2022 Sep 1;74(9):1353-1363. PMID: 35641094
J Appl Microbiol

Enhanced in vitro antimicrobial activity of amphotericin B with berberine against dual-species biofilms of Candida albicans and Staphylococcus aureus. [Abstract]

2021 Apr;130(4):1154-1172. PMID: 32996236
Cardiovasc Drugs Ther

Berberine Reverses Nitroglycerin Tolerance through Suppressing Protein Kinase C Alpha Activity in Vascular Smooth Muscle Cells. [Abstract]

2022 Aug;36(4):633-643. PMID: 34319490
Mol Reprod Dev

Effect of berberine combined with metformin on autophagy in polycystic ovary syndrome by regulating AMPK/AKT/mTOR pathway. [Abstract]

2024 Aug;91(8):e23768. PMID: 39155689
Toxicol In Vitro

Inhibitory potential of phytochemicals on species-specific breast cancer resistance protein transport activity. [Abstract]

2025 Dec:109:106128. PMID: 40796067
BMC Pharmacol Toxicol

Antitumor effects of erlotinib in combination with berberine in A431 cells. [Abstract]

2023 May 11;24(1):29. PMID: 37170144

Berberine hemisulfate purchased from MedChemExpress. Usage Cited in: BMC Pharmacol Toxicol. 2023 May 11;24(1):29. [Abstract]

Berberine (BBR; 2.5, 5, 10 µM; 48 h) inhibits EGFR phosphorylation in a dose-dependent matter in A431cells.

Berberine hemisulfate purchased from MedChemExpress. Usage Cited in: BMC Pharmacol Toxicol. 2023 May 11;24(1):29. [Abstract]

Berberine (BBR; 2.5 µM; 10 days) significantly inhibits NCI-H441 colony formation.

Berberine hemisulfate purchased from MedChemExpress. Usage Cited in: BMC Pharmacol Toxicol. 2023 May 11;24(1):29. [Abstract]

Berberine (BBR; 0.625, 1.25, 2.5, 5, 10 µM; 48 h) inhibits the viability of A431cells in a dose-dependent matter.
Tissue Cell

Overcoming osimertinib resistance in NSCLC: The role of Notch1 pathway inhibition by berberine. [Abstract]

2026 Jun:100:103365. PMID: 41687187
Gene

Pharmacotranscriptomic profiling of resistant triple-negative breast cancer cells treated with lapatinib and berberine shows upregulation of PI3K/Akt signaling under cytotoxic stress. [Abstract]

2022 Mar 30:816:146171. PMID: 35026293
J Bone Miner Metab

Protective effects of berberine on senile osteoporosis in mice. [Abstract]

2021 Sep;39(5):748-756. PMID: 33829324
Mol Genet Genomics

Berberine impedes the DNA damage repair to inhibit colorectal cancer by regulating the SOX17/TCF4/PIM3 axis. [Abstract]

2026 May 27;301(1):124. PMID: 42201413
Arch Oral Biol

Investigating the mechanism of berberine in inhibiting periodontitis progression using machine learning, molecular docking, and experimental validation. [Abstract]

2026 Mar:183:106496. PMID: 41544413
Biol Pharm Bull

Berberine Encapsulated in Exosomes Derived from Platelet-Rich Plasma Promotes Chondrogenic Differentiation of the Bone Marrow Mesenchymal Stem Cells via the Wnt/β-Catenin Pathway. [Abstract]

2022 Oct 1;45(10):1444-1451. PMID: 35858798
Lett Drug Des Discov

2025 Dec 13.
bioRxiv

Mechanistic Language Modeling and Oxygenated 3D Screening Reveal Berberine and Enzalutamide Synergy in Resistant Prostate Cancer. [Abstract]

2026 Jan 26:2026.01.24.701539. PMID: 41659618
Res Sq

2025 Apr 15.
Genome Instab Dis

2025 Mar.
SSRN

2023 Jan 27.
Research Square Preprint

2021 Sep.
Research Square Preprint

2021 Jun.
Evid Based Complement Alternat Med

Effects of Berberine on Circular RNA Expression Profiles in Human Gastric Cancer Cells. [Abstract]

2021 May 4:2021:6688629. PMID: 34055022

Solvent & Solubility

In Vitro:

H₂O : 200 mg/mL (520.29 mM; Need ultrasonic)

DMSO : 2 mg/mL (5.20 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.6015 mL

13.0073 mL

26.0146 mL

5 mM

0.5203 mL

2.6015 mL

5.2029 mL

10 mM

0.2601 mL

1.3007 mL

2.6015 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

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Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (281 KB)
SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Cai Y, et al. Berberine inhibits the growth of human colorectal adenocarcinoma in vitro and in vivo. J Nat Med. 2014 Jan;68(1):53-62. [Content Brief]

[2]. Cui HX, et al. Preparation and Evaluation of Antidiabetic Agents of Berberine Organic Acid Salts for Enhancing the Bioavailability. Molecules. 2018 Dec 28;24(1):103. [Content Brief]

[3]. Boberek JM, et al., Genetic evidence for inhibition of bacterial division protein FtsZ by berberine. PLoS One. 2010 Oct 29;5(10):e13745. [Content Brief]

[4]. Remppis A, et al., Rhizoma Coptidis inhibits LPS-induced MCP-1/CCL2 production in murine macrophages via an AP-1 and NFkappaB-dependent pathway. Mediators Inflamm. 2010;2010:194896. [Content Brief]

[5]. Bae J, et al., Berberine protects 6-hydroxydopamine-induced human dopaminergic neuronal cell death through the induction of heme oxygenase-1. Mol Cells. 2013 Feb;35(2):151-7. [Content Brief]

[6]. Ye Y, et al., Efficacy and Safety of Berberine Alone for Several Metabolic Disorders: A Systematic Review and Meta-Analysis of Randomized Clinical Trials. Front Pharmacol. 2021 Apr 26;12:653887. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO / H₂O	1 mM	2.6015 mL	13.0073 mL	26.0146 mL	65.0364 mL
DMSO / H₂O	5 mM	0.5203 mL	2.6015 mL	5.2029 mL	13.0073 mL
H₂O	10 mM	0.2601 mL	1.3007 mL	2.6015 mL	6.5036 mL
	15 mM	0.1734 mL	0.8672 mL	1.7343 mL	4.3358 mL
	20 mM	0.1301 mL	0.6504 mL	1.3007 mL	3.2518 mL
	25 mM	0.1041 mL	0.5203 mL	1.0406 mL	2.6015 mL
	30 mM	0.0867 mL	0.4336 mL	0.8672 mL	2.1679 mL
	40 mM	0.0650 mL	0.3252 mL	0.6504 mL	1.6259 mL
	50 mM	0.0520 mL	0.2601 mL	0.5203 mL	1.3007 mL
	60 mM	0.0434 mL	0.2168 mL	0.4336 mL	1.0839 mL
	80 mM	0.0325 mL	0.1626 mL	0.3252 mL	0.8130 mL
	100 mM	0.0260 mL	0.1301 mL	0.2601 mL	0.6504 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Berberine hemisulfate

53 Cited Publications

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Berberine hemisulfate

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