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  3. Berberine chloride hydrate

Berberine chloride hydrate (Synonyms: Natural Yellow 18 (chloride hydrate))

Cat. No.: HY-17577 Purity: 99.56%
Handling Instructions

Berberine chloride hydrate is an alkaloid isolated from the Chinese herbal medicine Huanglian, as an antibiotic. Berberine chloride hydrate induces reactive oxygen species (ROS) generation and inhibits DNA topoisomerase. Antineoplastic properties.

For research use only. We do not sell to patients.

Berberine chloride hydrate Chemical Structure

Berberine chloride hydrate Chemical Structure

CAS No. : 68030-18-2

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Based on 1 publication(s) in Google Scholar

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Description

Berberine chloride hydrate is an alkaloid isolated from the Chinese herbal medicine Huanglian, as an antibiotic. Berberine chloride hydrate induces reactive oxygen species (ROS) generation and inhibits DNA topoisomerase. Antineoplastic properties[1].

IC50 & Target

ROS[1]
DNA topoisomerase[1]

In Vitro

Berberine (1.25-160 μM; 72 hours) has potential inhibitory effects on the proliferation of four colorectal carcinoma cell lines LoVo, HCT116, SW480, and HT-29[1].
Berberine (1.25-160 μM; 24-72 hours) induces a time- and dose-dependent inhibition of LoVo cell growth[1].
LoVo cells are exposure to Berberine (10-80 μM) for 24 h. Cell cycle analysis of 40 μM Berberine-treated LoVo cells by flow cytometry shows accumulation of cells in the G2/M phase[1].
Berberine (10-80 μM) suppresses cyclin B1, cdc2 and cdc25c protein expression after 24 h, especially at the dose of 80.0 μM[1].

Cell Proliferation Assay[1]

Cell Line: Four colorectal carcinoma cell lines LoVo, HCT116, SW480, and HT-29
Concentration: 1.25, 2.5, 5, 10, 20, 40, 80, and 160 μM
Incubation Time: 72 hours
Result: Inhibited the proliferation of four cell lines. The IC50 ranged from 40.8±4.1 μM (LoVo) to 98.6±2.9 μM (HCT116).

Cell Proliferation Assay[1]

Cell Line: Colorectal carcinoma cell lines LoVo
Concentration: 1.25, 2.5, 5, 10, 20, 40, 80, and 160 μM
Incubation Time: 24, 48, 72 hours
Result: Induced a time- and dose-dependent inhibition of cell growth. By 72 h, 160.0 μM induced 71.1±1.9 % growth inhibitions in LoVo cells.

Cell Cycle Analysis[1]

Cell Line: LoVo cells
Concentration: 0, 10, 20, 40, or 80 μM
Incubation Time: 24 hours
Result: Exposure to 40.0 μM induced G2/M-phase cell cycle arrest, an increase in the G2/M-phase population and a progressive decline in the G1 population.

Western Blot Analysis[1]

Cell Line: LoVo cells
Concentration: 10, 20, 40, or 80 μM
Incubation Time: 24 hours
Result: Suppressed cyclin B1, cdc2 and cdc25c protein expression.
In Vivo

Berberine (10, 30, or 50 mg/kg/day; gastrointestinal gavage; for 10 consecutive days) inhibits the growth of human colorectal adenocarcinoma in vivo. Berberine at doses of 30 and 50 mg/kg/day taken by gastrointestinal gavage shows inhibitory rates of 33.1% and 45.3% on the human colorectal adenocarcinoma xenograft growth in nude mice[1].

Animal Model: 5-week-old BALB/c nu/nu mice with human colorectal adenocarcinoma LoVo xenografts[1]
Dosage: 10, 30, or 50 mg/kg/day
Administration: Gastrointestinal gavage; for 10 consecutive days
Result: Showed inhibitory rates of 33.1 % and 45.3 % at doses of 30 and 50 mg/kg/day.
Clinical Trial
Molecular Weight

389.83

Formula

C₂₀H₂₀ClNO₅

CAS No.

68030-18-2

SMILES

COC1=C(OC)C2=C[N+]3=C(C(C(CC3)=C4)=CC5=C4OCO5)C=C2C=C1.[Cl-].O

Shipping

Room temperature in continental US; may vary elsewhere

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 3.9 mg/mL (10.00 mM)

H2O : 1.25 mg/mL (3.21 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5652 mL 12.8261 mL 25.6522 mL
5 mM 0.5130 mL 2.5652 mL 5.1304 mL
10 mM 0.2565 mL 1.2826 mL 2.5652 mL
*Please refer to the solubility information to select the appropriate solvent.
References

Purity: 99.56%

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Berberine chloride hydrate
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