1. Cell Cycle/DNA Damage
    Autophagy
    Anti-infection
    NF-κB
    Metabolic Enzyme/Protease
    Immunology/Inflammation
  2. Topoisomerase
    Autophagy
    Bacterial
    Reactive Oxygen Species
  3. Berberine

Berberine (Synonyms: Natural Yellow 18)

Cat. No.: HY-N0716
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Berberine (Natural Yellow 18) is an alkaloid isolated from the Chinese herbal medicine Huanglian, as an antibiotic. Berberine (Natural Yellow 18) induces reactive oxygen species (ROS) generation and inhibits DNA topoisomerase. Berberine (Natural Yellow 18) has antineoplastic properties.

For research use only. We do not sell to patients.

Berberine Chemical Structure

Berberine Chemical Structure

CAS No. : 2086-83-1

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Description

Berberine (Natural Yellow 18) is an alkaloid isolated from the Chinese herbal medicine Huanglian, as an antibiotic. Berberine (Natural Yellow 18) induces reactive oxygen species (ROS) generation and inhibits DNA topoisomerase. Berberine (Natural Yellow 18) has antineoplastic properties[1].

IC50 & Target

ROS[1]
DNA topoisomerase[1]

In Vitro

Berberine (1.25-160 μM; 72 hours) has potential inhibitory effects on the proliferation of four colorectal carcinoma cell lines LoVo, HCT116, SW480, and HT-29[1].
Berberine (1.25-160 μM; 24-72 hours) induces a time- and dose-dependent inhibition of LoVo cell growth[1].
LoVo cells are exposure to Berberine (10-80 μM) for 24 h. Cell cycle analysis of 40 μM Berberine-treated LoVo cells by flow cytometry shows accumulation of cells in the G2/M phase[1].
Berberine (10-80 μM) suppresses cyclin B1, cdc2 and cdc25c protein expression after 24 h, especially at the dose of 80.0 μM[1].

Cell Proliferation Assay[1]

Cell Line: Four colorectal carcinoma cell lines LoVo, HCT116, SW480, and HT-29
Concentration: 1.25, 2.5, 5, 10, 20, 40, 80, and 160 μM
Incubation Time: 72 hours
Result: Inhibited the proliferation of four cell lines. The IC50 ranged from 40.8±4.1 μM (LoVo) to 98.6±2.9 μM (HCT116).

Cell Proliferation Assay[1]

Cell Line: Colorectal carcinoma cell lines LoVo
Concentration: 1.25, 2.5, 5, 10, 20, 40, 80, and 160 μM
Incubation Time: 24, 48, 72 hours
Result: Induced a time- and dose-dependent inhibition of cell growth. By 72 h, 160.0 μM induced 71.1±1.9 % growth inhibitions in LoVo cells.

Cell Cycle Analysis[1]

Cell Line: LoVo cells
Concentration: 0, 10, 20, 40, or 80 μM
Incubation Time: 24 hours
Result: Exposure to 40.0 μM induced G2/M-phase cell cycle arrest, an increase in the G2/M-phase population and a progressive decline in the G1 population.

Western Blot Analysis[1]

Cell Line: LoVo cells
Concentration: 10, 20, 40, or 80 μM
Incubation Time: 24 hours
Result: Suppressed cyclin B1, cdc2 and cdc25c protein expression.
In Vivo

Berberine (10, 30, or 50 mg/kg/day; gastrointestinal gavage; for 10 consecutive days) inhibits the growth of human colorectal adenocarcinoma in vivo. Berberine at doses of 30 and 50 mg/kg/day taken by gastrointestinal gavage shows inhibitory rates of 33.1% and 45.3% on the human colorectal adenocarcinoma xenograft growth in nude mice[1].

Animal Model: 5-week-old BALB/c nu/nu mice with human colorectal adenocarcinoma LoVo xenografts[1]
Dosage: 10, 30, or 50 mg/kg/day
Administration: Gastrointestinal gavage; for 10 consecutive days
Result: Showed inhibitory rates of 33.1 % and 45.3 % at doses of 30 and 50 mg/kg/day.
Molecular Weight

336.36

Formula

C₂₀H₁₈NO₄+

CAS No.

2086-83-1

SMILES

COC1=C(OC)C2=C[N+]3=C(C(C(CC3)=C4)=CC5=C4OCO5)C=C2C=C1

Shipping

Room temperature in continental US; may vary elsewhere

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

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