Cat. No.: HY-17463

Prednisolone

11 Cited Publications

Customer Review

Based on 11 publication(s) in Google Scholar

Prednisolone is a potent, orally active corticosteroid and a glucocorticoid. Prednisolone possesses about four times the anti-inflammatory activity of hydrocortisone while causing less salt and water retention. Prednisolone can be used for ocular, anti-inflammatory research.

For research use only. We do not sell to patients.

Purity: 99.93%
CAS No.: 50-24-8
Formula: C₂₁H₂₈O₅
Molecular Weight:360.44

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months

Prednisolone Chemical Structure

CAS No.: 50-24-8

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (11)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

All Endogenous Metabolite Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	EC₅₀	4.5 nM Compound: prednisolone	Ability to induce human IL-6 assay in A549 lung carcinoma cell line. Ability to induce human IL-6 assay in A549 lung carcinoma cell line.	[PMID: 15115388]
A549	EC₅₀	4.5 nM Compound: Prednisolone	GR-mediated transrepression of IL-6 in human A549 lung carcinoma cells GR-mediated transrepression of IL-6 in human A549 lung carcinoma cells	[PMID: 15808489]
A549	EC₅₀	4.5 nM Compound: Prednisolone	Activity at human glucocorticoid receptor in human A549 cells assessed as repression of human IL6 Activity at human glucocorticoid receptor in human A549 cells assessed as repression of human IL6	[PMID: 17467988]
A549	EC₅₀	13.6 nM Compound: pred	Transrepression activity at GR in IL-1-beta-stimulated human A549 cells assessed as inhibition of NF-kappaB-dependent E-selectin transcription by luciferase reporter gene assay Transrepression activity at GR in IL-1-beta-stimulated human A549 cells assessed as inhibition of NF-kappaB-dependent E-selectin transcription by luciferase reporter gene assay	[PMID: 19321341]
A549	EC₅₀	15.8 nM Compound: pred	Transrepression activity at GR in PMA-stimulated human A549 cells assessed as inhibition of AP1 response element-induced luciferase reporter gene activity Transrepression activity at GR in PMA-stimulated human A549 cells assessed as inhibition of AP1 response element-induced luciferase reporter gene activity	[PMID: 19321341]
A549	EC₅₀	0.002 μM Compound: 2, pred	Agonist activity at mineralocorticoid receptor in human A549 cells Agonist activity at mineralocorticoid receptor in human A549 cells	[PMID: 21073190]
A549	EC₅₀	13.6 nM Compound: 2, pred	Transrepression activity of glucocorticoid receptor in human A549 cells assessed as inhibition of IL1beta-stimulated NFkappaB-dependent E-selectin transcription by luciferase reporter gene assay Transrepression activity of glucocorticoid receptor in human A549 cells assessed as inhibition of IL1beta-stimulated NFkappaB-dependent E-selectin transcription by luciferase reporter gene assay	[PMID: 21073190]
A549	EC₅₀	15.8 nM Compound: 2, pred	Transrepression activity of glucocorticoid receptor in human A549 cells expressing AP-1 assessed as inhibition of PMA-induced AP-1 activity by luciferase reporter gene assay Transrepression activity of glucocorticoid receptor in human A549 cells expressing AP-1 assessed as inhibition of PMA-induced AP-1 activity by luciferase reporter gene assay	[PMID: 21073190]
A549	EC₅₀	0.002 nM Compound: 2, pred	Agonist activity at human mineralocorticoid receptor expressed in human A549 cells by fluorescence polarization assay Agonist activity at human mineralocorticoid receptor expressed in human A549 cells by fluorescence polarization assay	[PMID: 21899328]
A549	EC₅₀	14.4 nM Compound: 2, pred	Transrepression activity at glucocorticoid receptor alpha in IL-1beta-stimulated human A549 cells assessed as inhibition of NFkappaB-dependent E-selectin transcription by luciferase reporter gene assay Transrepression activity at glucocorticoid receptor alpha in IL-1beta-stimulated human A549 cells assessed as inhibition of NFkappaB-dependent E-selectin transcription by luciferase reporter gene assay	[PMID: 21899328]
A549	EC₅₀	16.1 nM Compound: 2, pred	Transrepression activity at glucocorticoid receptor alpha in phorbol myristate acetate-stimulated human A549 cells assessed as inhibition of AP1 response element by luciferase reporter gene assay Transrepression activity at glucocorticoid receptor alpha in phorbol myristate acetate-stimulated human A549 cells assessed as inhibition of AP1 response element by luciferase reporter gene assay	[PMID: 21899328]
A549	IC₅₀	5.8 nM Compound: Prednisolone	Antiinflammatory activity in human A549 cells assessed as inhibition of IL1beta-induced IL6 production preincubated for 30 mins followed by IL1beta-stimulation and measured after 20 hrs by AlphaLisa assay Antiinflammatory activity in human A549 cells assessed as inhibition of IL1beta-induced IL6 production preincubated for 30 mins followed by IL1beta-stimulation and measured after 20 hrs by AlphaLisa assay	[PMID: 30384041]
C2C12	EC₅₀	3.4 nM Compound: Prednisolone	Inhibition of mouse glutamine synthetase by GR-mediated transactivation in C2C12 cells Inhibition of mouse glutamine synthetase by GR-mediated transactivation in C2C12 cells	[PMID: 15808489]
C2C12	EC₅₀	3.4 nM Compound: Prednisolone	Activity at human glucocorticoid receptor in C2C12 cells assessed as mouse glutamine synthetase activity Activity at human glucocorticoid receptor in C2C12 cells assessed as mouse glutamine synthetase activity	[PMID: 17467988]
CV-1	IC₅₀	8 nM Compound: prednisolone	Transcriptional activation in CV-1 cells expressing glucocorticoid receptor Transcriptional activation in CV-1 cells expressing glucocorticoid receptor	[PMID: 11520196]
CV-1	EC₅₀	3.3 nM Compound: prednisolone	The effective concentration in CV-1 cells for glucocorticoid response element activation (GRE). The effective concentration in CV-1 cells for glucocorticoid response element activation (GRE).	[PMID: 11728194]
CV-1	EC₅₀	8 nM Compound: Prednisolone	Transcriptional activation in CV-1 cells expressing human glucocorticoid receptor Transcriptional activation in CV-1 cells expressing human glucocorticoid receptor	[PMID: 12620078]
CV-1	IC₅₀	2.1 nM Compound: Prednisolone	Inhibition of transcriptional repression in CV-1 cells expressing glucocorticoid receptor Inhibition of transcriptional repression in CV-1 cells expressing glucocorticoid receptor	[PMID: 15026058]
CV-1	IC₅₀	8 nM Compound: Prednisolone	Inhibition of transcriptional activation in CV-1 cells expressing glucocorticoid receptor Inhibition of transcriptional activation in CV-1 cells expressing glucocorticoid receptor	[PMID: 15026058]
CV-1	ED₅₀	4 nM Compound: prednisolone	Activity at human glucocorticoid receptor expressed in CV1 cells assessed as repression of TNF and IL-1-beta-induced E-selectin gene expression by luciferase reporter gene assay Activity at human glucocorticoid receptor expressed in CV1 cells assessed as repression of TNF and IL-1-beta-induced E-selectin gene expression by luciferase reporter gene assay	[PMID: 18032610]
CV-1	EC₅₀	5.3 nM Compound: 1	Agonist activity at GR expressed in african green monkey CV1 cells transfected with luciferase gene linked to MMTV promoter assessed as induction of luciferase transactivation activity relative to Dexamethasone Agonist activity at GR expressed in african green monkey CV1 cells transfected with luciferase gene linked to MMTV promoter assessed as induction of luciferase transactivation activity relative to Dexamethasone	[PMID: 21316964]
CV-1	EC₅₀	5.3 nM Compound: 1	Agonist activity at GR expressed in african green monkey CV1 cells transfected with luciferase gene linked to MMTV promoter assessed as induction of luciferase transactivation activity Agonist activity at GR expressed in african green monkey CV1 cells transfected with luciferase gene linked to MMTV promoter assessed as induction of luciferase transactivation activity	[PMID: 21349714]
HeLa	EC₅₀	16 nM Compound: 1b	Activation of MMTV in HeLa cells measured by luciferase activity Activation of MMTV in HeLa cells measured by luciferase activity	[PMID: 17181172]
HeLa	EC₅₀	82.7 nM Compound: 2, pred	Transactivation activity of glucocorticoid receptor ligand binding domain expressed in human NP-1 Hela cells co-expressing GAL4 DNA binding domain by luciferase reporter gene assay Transactivation activity of glucocorticoid receptor ligand binding domain expressed in human NP-1 Hela cells co-expressing GAL4 DNA binding domain by luciferase reporter gene assay	[PMID: 21073190]
HeLa	EC₅₀	21 nM Compound: 2, pred	Transactivation activity at GR-alpha in human NP1 Hela cells assessed as inhibition of GAL4-DBD after 20 hrs by luciferase reporter gene assay Transactivation activity at GR-alpha in human NP1 Hela cells assessed as inhibition of GAL4-DBD after 20 hrs by luciferase reporter gene assay	[PMID: 21899328]
HeLa	EC₅₀	73.9 nM Compound: 2, pred	Transactivation activity at GR-alpha in human NP1 Hela cells assessed as induction of GAL4-DBD after 20 hrs by luciferase reporter gene assay Transactivation activity at GR-alpha in human NP1 Hela cells assessed as induction of GAL4-DBD after 20 hrs by luciferase reporter gene assay	[PMID: 21899328]
HeLa	EC₅₀	16 nM Compound: Pred	Agonist activity at glucocorticoid receptor in human HeLa cells transfected with luciferase gene linked to MMTV promoter assessed as luciferase transactivation activity Agonist activity at glucocorticoid receptor in human HeLa cells transfected with luciferase gene linked to MMTV promoter assessed as luciferase transactivation activity	[PMID: 21963986]
HepG2	IC₅₀	2.1 nM Compound: prednisolone	Transcriptional repression activity in HEP G2 cells expressing glucocorticoid receptor compared to Dexamethasone Transcriptional repression activity in HEP G2 cells expressing glucocorticoid receptor compared to Dexamethasone	[PMID: 11520196]
HepG2	EC₅₀	2.6 nM Compound: prednisolone	Effective concentration in HepG2 cells transfected with LUC gene (E-sel-Luc). Effective concentration in HepG2 cells transfected with LUC gene (E-sel-Luc).	[PMID: 11728194]
HepG2	EC₅₀	2.1 nM Compound: Prednisolone	Transcriptional repression in HepG2 cells expressing human glucocorticoid receptor Transcriptional repression in HepG2 cells expressing human glucocorticoid receptor	[PMID: 12620078]
HepG2	EC₅₀	211 nM Compound: prednisolone	Glucocorticoid induced transactivation of human tyrosine amino transferase in HepG2 cells Glucocorticoid induced transactivation of human tyrosine amino transferase in HepG2 cells	[PMID: 15115388]
HepG2	EC₅₀	24 nM Compound: Prednisolone	Inhibition of GR-mediated tyrosine amino transferase activity in human HepG2 cells Inhibition of GR-mediated tyrosine amino transferase activity in human HepG2 cells	[PMID: 15808489]
HepG2	EC₅₀	24 nM Compound: Prednisolone	Activity at human glucocorticoid receptor in human HepG2 cells assessed as tyrosine amino transferase activity Activity at human glucocorticoid receptor in human HepG2 cells assessed as tyrosine amino transferase activity	[PMID: 17467988]
HepG2	EC₅₀	5.3 nM Compound: 1	Agonist activity at glucocorticoid receptor in human HepG2 cells co-transfected with GRE assessed as transactivation activity by luciferase reporter gene assay Agonist activity at glucocorticoid receptor in human HepG2 cells co-transfected with GRE assessed as transactivation activity by luciferase reporter gene assay	[PMID: 21115247]
HepG2	IC₅₀	23 nM Compound: 1	Transrepression activity at glucocorticoid receptor in IL-1beta-stimulated human HepG2 cells assessed as inhibition of AP1 response element-induced IL-6 production by ELISA Transrepression activity at glucocorticoid receptor in IL-1beta-stimulated human HepG2 cells assessed as inhibition of AP1 response element-induced IL-6 production by ELISA	[PMID: 21115247]
HepG2	IC₅₀	4.1 nM Compound: 1	Transrepression activity at glucocorticoid receptor in TNFalpha/IL1beta-stimulated human HepG2 cells assessed as inhibition of NFkappaB-dependent E-selectin transcription by luciferase reporter gene assay Transrepression activity at glucocorticoid receptor in TNFalpha/IL1beta-stimulated human HepG2 cells assessed as inhibition of NFkappaB-dependent E-selectin transcription by luciferase reporter gene assay	[PMID: 21115247]
HepG2	IC₅₀	4.1 nM Compound: 1	Agonist activity at GR expressed in IL-1beta- and TNFalpha-stimulated HepG2 cells assessed as inhibition of NFKB- or AP-1 mediated E-selectin transcription by luciferase reporter gene assay relative to Dexamethasone Agonist activity at GR expressed in IL-1beta- and TNFalpha-stimulated HepG2 cells assessed as inhibition of NFKB- or AP-1 mediated E-selectin transcription by luciferase reporter gene assay relative to Dexamethasone	[PMID: 21316964]
HepG2	EC₅₀	26 nM Compound: 1	Agonist activity at glucocorticoid receptor in human HepG2 cells co-transfected with PEPCK assessed as GRE activation by luciferase reporter gene assay Agonist activity at glucocorticoid receptor in human HepG2 cells co-transfected with PEPCK assessed as GRE activation by luciferase reporter gene assay	[PMID: 21324689]
HepG2	EC₅₀	5.3 nM Compound: 1	Agonist activity at glucocorticoid receptor in human HepG2 cells co-transfected with GRE assessed as GRE activation by luciferase reporter gene assay Agonist activity at glucocorticoid receptor in human HepG2 cells co-transfected with GRE assessed as GRE activation by luciferase reporter gene assay	[PMID: 21324689]
HepG2	IC₅₀	4.1 nM Compound: 1	Transrepression activity at glucocorticoid receptor in human HepG2 cells assessed as inhibition of TNFalpha/IL1beta-stimulated NFkappaB-dependent E-selectin repression by luciferase reporter gene assay Transrepression activity at glucocorticoid receptor in human HepG2 cells assessed as inhibition of TNFalpha/IL1beta-stimulated NFkappaB-dependent E-selectin repression by luciferase reporter gene assay	[PMID: 21324689]
HepG2	IC₅₀	4.1 nM Compound: 1	Transrepression activity at GR expressed in IL-1beta- and TNFalpha-stimulated HepG2 cells assessed as inhibition of NFKB- or AP-1 mediated E-selectin transcription by luciferase reporter gene assay Transrepression activity at GR expressed in IL-1beta- and TNFalpha-stimulated HepG2 cells assessed as inhibition of NFKB- or AP-1 mediated E-selectin transcription by luciferase reporter gene assay	[PMID: 21349714]
HepG2	IC₅₀	340 nM Compound: 2	Transactivation of glucocorticoid receptor in human HepG2 cells assessed as induction of TAT measuring degradation of tyrosine to p-hydroxy phenyl pyruvate Transactivation of glucocorticoid receptor in human HepG2 cells assessed as induction of TAT measuring degradation of tyrosine to p-hydroxy phenyl pyruvate	[PMID: 22197391]
HFF	EC₅₀	19 nM Compound: 1b	Induction of aromatase activity in HFF cells assessed as production of beta estradiol by aromatase assay Induction of aromatase activity in HFF cells assessed as production of beta estradiol by aromatase assay	[PMID: 17181172]
HFF	IC₅₀	6.6 nM Compound: 1b	Inhibition of IL1-stimulated IL6 production in HFF cells Inhibition of IL1-stimulated IL6 production in HFF cells	[PMID: 17181172]
HL-60	IC₅₀	0.76 μM Compound: Prednisolone	Inhibitory concentration against interleukin-1 beta production in HL-60 cells stimulated with lipopolysaccharide (LPS). Inhibitory concentration against interleukin-1 beta production in HL-60 cells stimulated with lipopolysaccharide (LPS).	[PMID: 11527733]
HL-60	IC₅₀	0.76 μM Compound: Prednisolone	Inhibitory concentration against interleukin-1 beta production in HL-60 cells stimulated with lipopolysaccharide (LPS). Inhibitory concentration against interleukin-1 beta production in HL-60 cells stimulated with lipopolysaccharide (LPS).	[PMID: 11527734]
Huh-7	EC₅₀	57.4 nM Compound: 2, pred	Transactivation activity at glucocorticoid receptor in human 13D3/Huh7 cells assessed as induction of tyrosine aminotransferase activity after 24 hrs Transactivation activity at glucocorticoid receptor in human 13D3/Huh7 cells assessed as induction of tyrosine aminotransferase activity after 24 hrs	[PMID: 21073190]
Huh-7	EC₅₀	64 nM Compound: 2, pred	Transactivation activity at glucocorticoid receptor alpha human 13D3/Huh7 cells assessed as induction of TAT activity after 4 hrs by spectrophotometry Transactivation activity at glucocorticoid receptor alpha human 13D3/Huh7 cells assessed as induction of TAT activity after 4 hrs by spectrophotometry	[PMID: 21899328]
L1210	ED₅₀	100 μM Compound: Prednisolone	Cytotoxicity against mouse L1210 cells assessed as growth inhibition measured at 72 hrs by trypan blue exclusion assay Cytotoxicity against mouse L1210 cells assessed as growth inhibition measured at 72 hrs by trypan blue exclusion assay	[PMID: 118258]
Lymphocyte	IC₅₀	0.078 μM Compound: Prednisolone	Immunosuppressive activity in rat lymphocytes assessed as inhibition of mixed-lymphocyte reaction Immunosuppressive activity in rat lymphocytes assessed as inhibition of mixed-lymphocyte reaction	[PMID: 19179074]
MG-63	EC₅₀	19 nM Compound: 2, pred	Induction of glutamine synthase expression in human MG63 cells after overnight incubation by spectrophotometry Induction of glutamine synthase expression in human MG63 cells after overnight incubation by spectrophotometry	[PMID: 21899328]
NHDF	IC₅₀	23 nM Compound: 1	Agonist activity at human GR expressed in NHDF cells assessed as inhibition of IL-6 production by ELISA relative to Dexamethasone Agonist activity at human GR expressed in NHDF cells assessed as inhibition of IL-6 production by ELISA relative to Dexamethasone	[PMID: 21316964]
NHDF	IC₅₀	23 nM Compound: 1	Transrepression activity at glucocorticoid receptor in human NHDF cells assessed as inhibition of IL-1beta-stimulated AP1 dependent IL-6 repression by ELISA Transrepression activity at glucocorticoid receptor in human NHDF cells assessed as inhibition of IL-1beta-stimulated AP1 dependent IL-6 repression by ELISA	[PMID: 21324689]
NHDF	IC₅₀	23 nM Compound: 1	Transrepression activity at GR expressed in NHDF cells assessed as IL-1beta-mediated IL-6 transcription by ELISA Transrepression activity at GR expressed in NHDF cells assessed as IL-1beta-mediated IL-6 transcription by ELISA	[PMID: 21349714]
OVCAR-3	IC₅₀	1.1 μM Compound: 35	Cytotoxicity against human OVCAR-3 cells incubated for 72 hrs by SRB assay Cytotoxicity against human OVCAR-3 cells incubated for 72 hrs by SRB assay	[PMID: 32283298]
OVCAR-4	IC₅₀	1.1 μM Compound: 35	Cytotoxicity against human OVCAR-4 cells incubated for 72 hrs by SRB assay Cytotoxicity against human OVCAR-4 cells incubated for 72 hrs by SRB assay	[PMID: 32283298]
PBMC	IC₅₀	13 nM Compound: Pred	Antiinflammatory activity in Wistar rat PBMC assessed as inhibition of LPS-induced TNFalpha release preincubated for 45 mins followed by LPS addition after 18 hrs by ELISA Antiinflammatory activity in Wistar rat PBMC assessed as inhibition of LPS-induced TNFalpha release preincubated for 45 mins followed by LPS addition after 18 hrs by ELISA	[PMID: 27810243]
PBMC	IC₅₀	45 nM Compound: Pred	Antiinflammatory activity in human primary PBMC assessed as inhibition of LPS-induced TNFalpha release preincubated for 45 mins followed by LPS addition after 18 hrs by ELISA Antiinflammatory activity in human primary PBMC assessed as inhibition of LPS-induced TNFalpha release preincubated for 45 mins followed by LPS addition after 18 hrs by ELISA	[PMID: 27810243]
RAW264.7	IC₅₀	25.4 μM Compound: Prednisolone	Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced TNFalpha production after 4 hrs by ELISA Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced TNFalpha production after 4 hrs by ELISA	[PMID: 11000020]
T47D	IC₅₀	>1000 nM Compound: Prednisolone	Displacement of [3H]progesterone from progesterone receptor in human T47D cells after 20 hrs by scintillation counting method Displacement of [3H]progesterone from progesterone receptor in human T47D cells after 20 hrs by scintillation counting method	[PMID: 30384041]
U-937	IC₅₀	8.2 nM Compound: pred	Antiinflammatory activity in PMA-differentiated human U937 cells assessed as inhibition of LPS-induced TNFalpha production incubated for 60 mins prior to LPS challenge measured after 24 hrs Antiinflammatory activity in PMA-differentiated human U937 cells assessed as inhibition of LPS-induced TNFalpha production incubated for 60 mins prior to LPS challenge measured after 24 hrs	[PMID: 24446728]
U-937	IC₅₀	0.44 μM Compound: Prednisolone	Inhibition of LPS-induced IL-1beta expression in human U-937 cells preincubated for 1 hr followed by LPS addition and measured after 24 hrs by ELISA Inhibition of LPS-induced IL-1beta expression in human U-937 cells preincubated for 1 hr followed by LPS addition and measured after 24 hrs by ELISA	[PMID: 31883489]
U-937	IC₅₀	0.64 μM Compound: Prednisolone	Inhibition of LPS-induced TNF-alpha expression in human U-937 cells preincubated for 1 hr followed by LPS addition and measured after 24 hrs by ELISA Inhibition of LPS-induced TNF-alpha expression in human U-937 cells preincubated for 1 hr followed by LPS addition and measured after 24 hrs by ELISA	[PMID: 31883489]
U-937	IC₅₀	0.033 μM Compound: Prednisolone	Antiinflammatory activity in human U937 cells assessed as inhibition of PMA/LPS-stimulated TNFalpha production after 24 hrs by ELISA Antiinflammatory activity in human U937 cells assessed as inhibition of PMA/LPS-stimulated TNFalpha production after 24 hrs by ELISA	[PMID: 32631542]

In Vitro

Prednisolone (0.002-10 μg/mL; 3 days) inhibits human leukocyte mitosis^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Prednisolone (5 mg/kg/day; p.o.; 6 days a week for 72 weeks) significantly prevents deaths from renal disease in mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

NZB/NZW mice, immune nephritis model^[3]
Dosage:

5 mg/kg/day
Administration:

Oral, 6 days a week for 72 weeks
Result:

Significantly lowered mortality rate and prolonged life significantly.

Chemical Information

CAS No. 50-24-8
Appearance Solid
Molecular Weight 360.44
Formula C₂₁H₂₈O₅
Color White to off-white
SMILES

C[C@@]1([C@@]2(O)C(CO)=O)[C@](CC2)([H])[C@@](CCC3=CC4=O)([H])[C@]([C@]3(C=C4)C)([H])[C@@H](O)C1
Structure Classification
- Steroids
Initial Source
- Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Publications (11)

All RT-PCRHistological Imaging/StainingIFCell Imaging/Staining

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Journal Impact Factor

Adv Sci (Weinh)

Hepatocyte BDNF Acts as a Novel Immune Checkpoint to Restrain TLR4-Mediated Acute Hepatitis. [Abstract]

2026 Mar 25:e21164. PMID: 41881032
Gut Microbes

Targeted modulation of intestinal barrier and mucosal immune-related microbiota attenuates IgA nephropathy progression. [Abstract]

2025 Dec;17(1):2458184. PMID: 39875350

Prednisolone purchased from MedChemExpress. Usage Cited in: Gut Microbes. 2025 Dec;17(1):2458184. [Abstract]

Positive (Prednisolone) group (5 mg/kg BW). Lomerular IgA, C3, and IgG immunofluorescence analysis.

Prednisolone purchased from MedChemExpress. Usage Cited in: Gut Microbes. 2025 Dec;17(1):2458184. [Abstract]

Positive (Prednisolone) group (5 mg/kg BW). H&E staining of colon tissue (scale bars = 100 μm and 50 μm, respectively) (n = 3).
Cell Commun Signal

Increased HERV-E clone 4-1 expression contributes to DNA hypomethylation and IL-17 release from CD4+ T cells via miR-302d/MBD2 in systemic lupus erythematosus. [Abstract]

2019 Aug 14;17(1):94. PMID: 31412880

Prednisolone purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2019 Aug 14;17(1):94. [Abstract]

CD4⁺ T cells from SLE patient were treated with TNF-α, IL-6, E2, LPS, UVB, prednisolone (10 ng/ml, 24 h) in vitro.
Int J Biol Macromol

Targeting local glucocorticoid metabolism with a silk fibroin microneedle for more effective hypertrophic scar repair. [Abstract]

2025 Jul 4;319(Pt 4):145753. PMID: 40617420

Prednisolone purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Jul 4;319(Pt 4):145753. [Abstract]

H&E and Masson's trichrome staining analysis showed that post 7 days treatment, the arrangement of collagen fibers became looser, and the thickness of epidermis was found thinner in either prednisone or prednisolone (650-1000 μm) SF-MN groups compared to the control with no treatment.

Prednisolone purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Jul 4;319(Pt 4):145753. [Abstract]

prednisolone (650-1000 μm). CCK-8 assay of MSF cells and human HaCaT cells.
Cell Rep Methods

RECOVER identifies synergistic drug combinations in vitro through sequential model optimization. [Abstract]

2023 Oct 23;3(10):100599. PMID: 37797618
Cancers (Basel)

Association between Dysfunction of the Nucleolar Stress Response and Multidrug Resistance in Pediatric Acute Lymphoblastic Leukemia. [Abstract]

2022 Oct 19;14(20):5127. PMID: 36291909
Environ Toxicol Pharmacol

Assessment of the toxicity of acrolein on an alveolus microphysiological system. [Abstract]

2026 Mar:122:104954. PMID: 41644025
Chem Biol Drug Des

Potential Modulatory Effects of Hesperidin on the JAK/STAT Pathway in Mesangial Proliferative Glomerulonephritis. [Abstract]

2025 Feb;105(2):e70032. PMID: 39928525
Drug Test Anal

High-throughput liquid chromatography tandem mass spectrometry assay as initial testing procedure for analysis of total urinary fraction. [Abstract]

2021 Feb;13(2):283-298. PMID: 32852861
Chem Biodivers

Integration of Zebrafish Model and Network Pharmacology to Explore Possible Action Mechanisms of Morinda officinalis for Treating Osteoporosis. [Abstract]

2020 May;17(5):e2000056. PMID: 32190963
bioRxiv

Closing the loop: Teaching single-cell foundation models to learn from perturbations. [Abstract]

2025 Jul 12:2025.07.08.663754. PMID: 40672312

Solvent & Solubility

In Vitro:

DMSO : 62.5 mg/mL (173.40 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 0.1 mg/mL (0.28 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.7744 mL

13.8719 mL

27.7439 mL

5 mM

0.5549 mL

2.7744 mL

5.5488 mL

10 mM

0.2774 mL

1.3872 mL

2.7744 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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Volume _(start) Volume _(start)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (5.77 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (5.77 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (5.77 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (761 KB)
- English - EN (761 KB)
- Français - FR (761 KB)
- Deutsch - DE (761 KB)
- Norwegian - NO (761 KB)
- Español - ES (761 KB)
- Swedish - SV (761 KB)
- Italian - IT (761 KB)
- Portuguese - PT (761 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Prednisolone. J Am Pharm Assoc. 1976;16(3):143-146. [Content Brief]

[2]. Musson DG, et al. Assay methodology for prednisolone, prednisolone acetate and prednisolone sodium phosphate in rabbit aqueous humor and ocular physiological solutions. J Chromatogr. 1991;565(1-2):89-102. [Content Brief]

[3]. Hahn B H, et al. Comparison of therapeutic and immunosuppressive effects of azathioprine, prednisolone and combined therapy in nzp/nzw mice. Arthritis & Rheumatism: Official Journal of the American College of Rheumatology, 1973, 16(2): 163-170.

[4]. NOWELL PC. Inhibition of human leukocyte mitosis by prednisolone in vitro. Cancer Res. 1961 Dec;21:1518-21. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.7744 mL	13.8719 mL	27.7439 mL	69.3597 mL
	5 mM	0.5549 mL	2.7744 mL	5.5488 mL	13.8719 mL
	10 mM	0.2774 mL	1.3872 mL	2.7744 mL	6.9360 mL
	15 mM	0.1850 mL	0.9248 mL	1.8496 mL	4.6240 mL
	20 mM	0.1387 mL	0.6936 mL	1.3872 mL	3.4680 mL
	25 mM	0.1110 mL	0.5549 mL	1.1098 mL	2.7744 mL
	30 mM	0.0925 mL	0.4624 mL	0.9248 mL	2.3120 mL
	40 mM	0.0694 mL	0.3468 mL	0.6936 mL	1.7340 mL
	50 mM	0.0555 mL	0.2774 mL	0.5549 mL	1.3872 mL
	60 mM	0.0462 mL	0.2312 mL	0.4624 mL	1.1560 mL
	80 mM	0.0347 mL	0.1734 mL	0.3468 mL	0.8670 mL
	100 mM	0.0277 mL	0.1387 mL	0.2774 mL	0.6936 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Prednisolone

11 Cited Publications

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Prednisolone

Immunology/Inflammation Compound Library

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Publications Citing Use of MedChemExpress (MCE) Prednisolone

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Publications (11)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Prednisolone Related Classifications

Cat. No.: HY-17463