Prednisolone

Name: Prednisolone
Brand: MedChemExpress
SKU: HY-17463
Rating: 5.0 (11 reviews)

Cat. No.: HY-17463 Purity: 99.93%: Data Sheet
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Prednisolone is a potent, orally active corticosteroid and a glucocorticoid. Prednisolone possesses about four times the anti-inflammatory activity of hydrocortisone while causing less salt and water retention. Prednisolone can be used for ocular, anti-inflammatory research.

For research use only. We do not sell to patients.

CAS No. : 50-24-8

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	In-stock	Estimated Time of Arrival: December 31
25 g	In-stock	Estimated Time of Arrival: December 31
50 g	Get quote

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Customer Review

Based on 11 publication(s) in Google Scholar

Other Forms of Prednisolone:

Prednisolone-d₇ In-stock
Prednisolone disodium phosphate In-stock
Prednisolone hemisuccinate In-stock
Prednisolone acetate In-stock
Prednisolone (Standard) In-stock
Prednisolone-d₈ Get quote
Prednisolone-d₆ Get quote
Prednisolone-d₄-1 Get quote

11 Publications Citing Use of MCE Prednisolone

RT-PCR

Histological Imaging/Staining

Cell Imaging/Staining

: Prednisolone purchased from MedChemExpress. Usage Cited in: Gut Microbes. 2025 Dec;17(1):2458184. [Abstract]; Positive (Prednisolone) group (5 mg/kg BW). Lomerular IgA, C3, and IgG immunofluorescence analysis.

: Prednisolone purchased from MedChemExpress. Usage Cited in: Gut Microbes. 2025 Dec;17(1):2458184. [Abstract]; Positive (Prednisolone) group (5 mg/kg BW). H&E staining of colon tissue (scale bars = 100 μm and 50 μm, respectively) (n = 3).

: Prednisolone purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Jul 4;319(Pt 4):145753. [Abstract]; H&E and Masson's trichrome staining analysis showed that post 7 days treatment, the arrangement of collagen fibers became looser, and the thickness of epidermis was found thinner in either prednisone or prednisolone (650-1000 μm) SF-MN groups compared to the control with no treatment.

: Prednisolone purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Jul 4;319(Pt 4):145753. [Abstract]; prednisolone (650-1000 μm). CCK-8 assay of MSF cells and human HaCaT cells.

: Prednisolone purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2019 Aug 14;17(1):94. [Abstract]; CD4⁺ T cells from SLE patient were treated with TNF-α, IL-6, E2, LPS, UVB, prednisolone (10 ng/ml, 24 h) in vitro.

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Biological Activity
Purity & Documentation
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	EC₅₀	0.002 nM Compound: 2, pred	Agonist activity at human mineralocorticoid receptor expressed in human A549 cells by fluorescence polarization assay Agonist activity at human mineralocorticoid receptor expressed in human A549 cells by fluorescence polarization assay	[PMID: 21899328]
A549	EC₅₀	0.002 μM Compound: 2, pred	Agonist activity at mineralocorticoid receptor in human A549 cells Agonist activity at mineralocorticoid receptor in human A549 cells	[PMID: 21073190]
A549	EC₅₀	13.6 nM Compound: 2, pred	Transrepression activity of glucocorticoid receptor in human A549 cells assessed as inhibition of IL1beta-stimulated NFkappaB-dependent E-selectin transcription by luciferase reporter gene assay Transrepression activity of glucocorticoid receptor in human A549 cells assessed as inhibition of IL1beta-stimulated NFkappaB-dependent E-selectin transcription by luciferase reporter gene assay	[PMID: 21073190]
A549	EC₅₀	13.6 nM Compound: pred	Transrepression activity at GR in IL-1-beta-stimulated human A549 cells assessed as inhibition of NF-kappaB-dependent E-selectin transcription by luciferase reporter gene assay Transrepression activity at GR in IL-1-beta-stimulated human A549 cells assessed as inhibition of NF-kappaB-dependent E-selectin transcription by luciferase reporter gene assay	[PMID: 19321341]
A549	EC₅₀	14.4 nM Compound: 2, pred	Transrepression activity at glucocorticoid receptor alpha in IL-1beta-stimulated human A549 cells assessed as inhibition of NFkappaB-dependent E-selectin transcription by luciferase reporter gene assay Transrepression activity at glucocorticoid receptor alpha in IL-1beta-stimulated human A549 cells assessed as inhibition of NFkappaB-dependent E-selectin transcription by luciferase reporter gene assay	[PMID: 21899328]
A549	EC₅₀	15.8 nM Compound: 2, pred	Transrepression activity of glucocorticoid receptor in human A549 cells expressing AP-1 assessed as inhibition of PMA-induced AP-1 activity by luciferase reporter gene assay Transrepression activity of glucocorticoid receptor in human A549 cells expressing AP-1 assessed as inhibition of PMA-induced AP-1 activity by luciferase reporter gene assay	[PMID: 21073190]
A549	EC₅₀	15.8 nM Compound: pred	Transrepression activity at GR in PMA-stimulated human A549 cells assessed as inhibition of AP1 response element-induced luciferase reporter gene activity Transrepression activity at GR in PMA-stimulated human A549 cells assessed as inhibition of AP1 response element-induced luciferase reporter gene activity	[PMID: 19321341]
A549	EC₅₀	16.1 nM Compound: 2, pred	Transrepression activity at glucocorticoid receptor alpha in phorbol myristate acetate-stimulated human A549 cells assessed as inhibition of AP1 response element by luciferase reporter gene assay Transrepression activity at glucocorticoid receptor alpha in phorbol myristate acetate-stimulated human A549 cells assessed as inhibition of AP1 response element by luciferase reporter gene assay	[PMID: 21899328]
A549	EC₅₀	4.5 nM Compound: Prednisolone	GR-mediated transrepression of IL-6 in human A549 lung carcinoma cells GR-mediated transrepression of IL-6 in human A549 lung carcinoma cells	[PMID: 15808489]
A549	EC₅₀	4.5 nM Compound: Prednisolone	Activity at human glucocorticoid receptor in human A549 cells assessed as repression of human IL6 Activity at human glucocorticoid receptor in human A549 cells assessed as repression of human IL6	[PMID: 17467988]
A549	EC₅₀	4.5 nM Compound: prednisolone	Ability to induce human IL-6 assay in A549 lung carcinoma cell line. Ability to induce human IL-6 assay in A549 lung carcinoma cell line.	[PMID: 15115388]
A549	IC₅₀	5.8 nM Compound: Prednisolone	Antiinflammatory activity in human A549 cells assessed as inhibition of IL1beta-induced IL6 production preincubated for 30 mins followed by IL1beta-stimulation and measured after 20 hrs by AlphaLisa assay Antiinflammatory activity in human A549 cells assessed as inhibition of IL1beta-induced IL6 production preincubated for 30 mins followed by IL1beta-stimulation and measured after 20 hrs by AlphaLisa assay	[PMID: 30384041]
C2C12	EC₅₀	3.4 nM Compound: Prednisolone	Inhibition of mouse glutamine synthetase by GR-mediated transactivation in C2C12 cells Inhibition of mouse glutamine synthetase by GR-mediated transactivation in C2C12 cells	[PMID: 15808489]
C2C12	EC₅₀	3.4 nM Compound: Prednisolone	Activity at human glucocorticoid receptor in C2C12 cells assessed as mouse glutamine synthetase activity Activity at human glucocorticoid receptor in C2C12 cells assessed as mouse glutamine synthetase activity	[PMID: 17467988]
CV-1	EC₅₀	3.3 nM Compound: prednisolone	The effective concentration in CV-1 cells for glucocorticoid response element activation (GRE). The effective concentration in CV-1 cells for glucocorticoid response element activation (GRE).	[PMID: 11728194]
CV-1	EC₅₀	5.3 nM Compound: 1	Agonist activity at GR expressed in african green monkey CV1 cells transfected with luciferase gene linked to MMTV promoter assessed as induction of luciferase transactivation activity relative to Dexamethasone Agonist activity at GR expressed in african green monkey CV1 cells transfected with luciferase gene linked to MMTV promoter assessed as induction of luciferase transactivation activity relative to Dexamethasone	[PMID: 21316964]
CV-1	EC₅₀	5.3 nM Compound: 1	Agonist activity at GR expressed in african green monkey CV1 cells transfected with luciferase gene linked to MMTV promoter assessed as induction of luciferase transactivation activity Agonist activity at GR expressed in african green monkey CV1 cells transfected with luciferase gene linked to MMTV promoter assessed as induction of luciferase transactivation activity	[PMID: 21349714]
CV-1	EC₅₀	8 nM Compound: Prednisolone	Transcriptional activation in CV-1 cells expressing human glucocorticoid receptor Transcriptional activation in CV-1 cells expressing human glucocorticoid receptor	[PMID: 12620078]
CV-1	ED₅₀	4 nM Compound: prednisolone	Activity at human glucocorticoid receptor expressed in CV1 cells assessed as repression of TNF and IL-1-beta-induced E-selectin gene expression by luciferase reporter gene assay Activity at human glucocorticoid receptor expressed in CV1 cells assessed as repression of TNF and IL-1-beta-induced E-selectin gene expression by luciferase reporter gene assay	[PMID: 18032610]
CV-1	IC₅₀	2.1 nM Compound: Prednisolone	Inhibition of transcriptional repression in CV-1 cells expressing glucocorticoid receptor Inhibition of transcriptional repression in CV-1 cells expressing glucocorticoid receptor	[PMID: 15026058]
CV-1	IC₅₀	8 nM Compound: Prednisolone	Inhibition of transcriptional activation in CV-1 cells expressing glucocorticoid receptor Inhibition of transcriptional activation in CV-1 cells expressing glucocorticoid receptor	[PMID: 15026058]
CV-1	IC₅₀	8 nM Compound: prednisolone	Transcriptional activation in CV-1 cells expressing glucocorticoid receptor Transcriptional activation in CV-1 cells expressing glucocorticoid receptor	[PMID: 11520196]
HFF	EC₅₀	19 nM Compound: 1b	Induction of aromatase activity in HFF cells assessed as production of beta estradiol by aromatase assay Induction of aromatase activity in HFF cells assessed as production of beta estradiol by aromatase assay	[PMID: 17181172]
HFF	IC₅₀	6.6 nM Compound: 1b	Inhibition of IL1-stimulated IL6 production in HFF cells Inhibition of IL1-stimulated IL6 production in HFF cells	[PMID: 17181172]
HL-60	IC₅₀	0.76 μM Compound: Prednisolone	Inhibitory concentration against interleukin-1 beta production in HL-60 cells stimulated with lipopolysaccharide (LPS). Inhibitory concentration against interleukin-1 beta production in HL-60 cells stimulated with lipopolysaccharide (LPS).	[PMID: 11527733]
HL-60	IC₅₀	0.76 μM Compound: Prednisolone	Inhibitory concentration against interleukin-1 beta production in HL-60 cells stimulated with lipopolysaccharide (LPS). Inhibitory concentration against interleukin-1 beta production in HL-60 cells stimulated with lipopolysaccharide (LPS).	[PMID: 11527734]
HeLa	EC₅₀	16 nM Compound: 1b	Activation of MMTV in HeLa cells measured by luciferase activity Activation of MMTV in HeLa cells measured by luciferase activity	[PMID: 17181172]
HeLa	EC₅₀	16 nM Compound: Pred	Agonist activity at glucocorticoid receptor in human HeLa cells transfected with luciferase gene linked to MMTV promoter assessed as luciferase transactivation activity Agonist activity at glucocorticoid receptor in human HeLa cells transfected with luciferase gene linked to MMTV promoter assessed as luciferase transactivation activity	[PMID: 21963986]
HeLa	EC₅₀	21 nM Compound: 2, pred	Transactivation activity at GR-alpha in human NP1 Hela cells assessed as inhibition of GAL4-DBD after 20 hrs by luciferase reporter gene assay Transactivation activity at GR-alpha in human NP1 Hela cells assessed as inhibition of GAL4-DBD after 20 hrs by luciferase reporter gene assay	[PMID: 21899328]
HeLa	EC₅₀	73.9 nM Compound: 2, pred	Transactivation activity at GR-alpha in human NP1 Hela cells assessed as induction of GAL4-DBD after 20 hrs by luciferase reporter gene assay Transactivation activity at GR-alpha in human NP1 Hela cells assessed as induction of GAL4-DBD after 20 hrs by luciferase reporter gene assay	[PMID: 21899328]
HeLa	EC₅₀	82.7 nM Compound: 2, pred	Transactivation activity of glucocorticoid receptor ligand binding domain expressed in human NP-1 Hela cells co-expressing GAL4 DNA binding domain by luciferase reporter gene assay Transactivation activity of glucocorticoid receptor ligand binding domain expressed in human NP-1 Hela cells co-expressing GAL4 DNA binding domain by luciferase reporter gene assay	[PMID: 21073190]
HepG2	EC₅₀	2.1 nM Compound: Prednisolone	Transcriptional repression in HepG2 cells expressing human glucocorticoid receptor Transcriptional repression in HepG2 cells expressing human glucocorticoid receptor	[PMID: 12620078]
HepG2	EC₅₀	2.6 nM Compound: prednisolone	Effective concentration in HepG2 cells transfected with LUC gene (E-sel-Luc). Effective concentration in HepG2 cells transfected with LUC gene (E-sel-Luc).	[PMID: 11728194]
HepG2	EC₅₀	211 nM Compound: prednisolone	Glucocorticoid induced transactivation of human tyrosine amino transferase in HepG2 cells Glucocorticoid induced transactivation of human tyrosine amino transferase in HepG2 cells	[PMID: 15115388]
HepG2	EC₅₀	24 nM Compound: Prednisolone	Inhibition of GR-mediated tyrosine amino transferase activity in human HepG2 cells Inhibition of GR-mediated tyrosine amino transferase activity in human HepG2 cells	[PMID: 15808489]
HepG2	EC₅₀	24 nM Compound: Prednisolone	Activity at human glucocorticoid receptor in human HepG2 cells assessed as tyrosine amino transferase activity Activity at human glucocorticoid receptor in human HepG2 cells assessed as tyrosine amino transferase activity	[PMID: 17467988]
HepG2	EC₅₀	26 nM Compound: 1	Agonist activity at glucocorticoid receptor in human HepG2 cells co-transfected with PEPCK assessed as GRE activation by luciferase reporter gene assay Agonist activity at glucocorticoid receptor in human HepG2 cells co-transfected with PEPCK assessed as GRE activation by luciferase reporter gene assay	[PMID: 21324689]
HepG2	EC₅₀	5.3 nM Compound: 1	Agonist activity at glucocorticoid receptor in human HepG2 cells co-transfected with GRE assessed as transactivation activity by luciferase reporter gene assay Agonist activity at glucocorticoid receptor in human HepG2 cells co-transfected with GRE assessed as transactivation activity by luciferase reporter gene assay	[PMID: 21115247]
HepG2	EC₅₀	5.3 nM Compound: 1	Agonist activity at glucocorticoid receptor in human HepG2 cells co-transfected with GRE assessed as GRE activation by luciferase reporter gene assay Agonist activity at glucocorticoid receptor in human HepG2 cells co-transfected with GRE assessed as GRE activation by luciferase reporter gene assay	[PMID: 21324689]
HepG2	IC₅₀	2.1 nM Compound: prednisolone	Transcriptional repression activity in HEP G2 cells expressing glucocorticoid receptor compared to Dexamethasone Transcriptional repression activity in HEP G2 cells expressing glucocorticoid receptor compared to Dexamethasone	[PMID: 11520196]
HepG2	IC₅₀	23 nM Compound: 1	Transrepression activity at glucocorticoid receptor in IL-1beta-stimulated human HepG2 cells assessed as inhibition of AP1 response element-induced IL-6 production by ELISA Transrepression activity at glucocorticoid receptor in IL-1beta-stimulated human HepG2 cells assessed as inhibition of AP1 response element-induced IL-6 production by ELISA	[PMID: 21115247]
HepG2	IC₅₀	340 nM Compound: 2	Transactivation of glucocorticoid receptor in human HepG2 cells assessed as induction of TAT measuring degradation of tyrosine to p-hydroxy phenyl pyruvate Transactivation of glucocorticoid receptor in human HepG2 cells assessed as induction of TAT measuring degradation of tyrosine to p-hydroxy phenyl pyruvate	[PMID: 22197391]
HepG2	IC₅₀	4.1 nM Compound: 1	Transrepression activity at glucocorticoid receptor in TNFalpha/IL1beta-stimulated human HepG2 cells assessed as inhibition of NFkappaB-dependent E-selectin transcription by luciferase reporter gene assay Transrepression activity at glucocorticoid receptor in TNFalpha/IL1beta-stimulated human HepG2 cells assessed as inhibition of NFkappaB-dependent E-selectin transcription by luciferase reporter gene assay	[PMID: 21115247]
HepG2	IC₅₀	4.1 nM Compound: 1	Agonist activity at GR expressed in IL-1beta- and TNFalpha-stimulated HepG2 cells assessed as inhibition of NFKB- or AP-1 mediated E-selectin transcription by luciferase reporter gene assay relative to Dexamethasone Agonist activity at GR expressed in IL-1beta- and TNFalpha-stimulated HepG2 cells assessed as inhibition of NFKB- or AP-1 mediated E-selectin transcription by luciferase reporter gene assay relative to Dexamethasone	[PMID: 21316964]
HepG2	IC₅₀	4.1 nM Compound: 1	Transrepression activity at glucocorticoid receptor in human HepG2 cells assessed as inhibition of TNFalpha/IL1beta-stimulated NFkappaB-dependent E-selectin repression by luciferase reporter gene assay Transrepression activity at glucocorticoid receptor in human HepG2 cells assessed as inhibition of TNFalpha/IL1beta-stimulated NFkappaB-dependent E-selectin repression by luciferase reporter gene assay	[PMID: 21324689]
HepG2	IC₅₀	4.1 nM Compound: 1	Transrepression activity at GR expressed in IL-1beta- and TNFalpha-stimulated HepG2 cells assessed as inhibition of NFKB- or AP-1 mediated E-selectin transcription by luciferase reporter gene assay Transrepression activity at GR expressed in IL-1beta- and TNFalpha-stimulated HepG2 cells assessed as inhibition of NFKB- or AP-1 mediated E-selectin transcription by luciferase reporter gene assay	[PMID: 21349714]
Huh-7	EC₅₀	57.4 nM Compound: 2, pred	Transactivation activity at glucocorticoid receptor in human 13D3/Huh7 cells assessed as induction of tyrosine aminotransferase activity after 24 hrs Transactivation activity at glucocorticoid receptor in human 13D3/Huh7 cells assessed as induction of tyrosine aminotransferase activity after 24 hrs	[PMID: 21073190]
Huh-7	EC₅₀	64 nM Compound: 2, pred	Transactivation activity at glucocorticoid receptor alpha human 13D3/Huh7 cells assessed as induction of TAT activity after 4 hrs by spectrophotometry Transactivation activity at glucocorticoid receptor alpha human 13D3/Huh7 cells assessed as induction of TAT activity after 4 hrs by spectrophotometry	[PMID: 21899328]
L1210	ED₅₀	100 μM Compound: Prednisolone	Cytotoxicity against mouse L1210 cells assessed as growth inhibition measured at 72 hrs by trypan blue exclusion assay Cytotoxicity against mouse L1210 cells assessed as growth inhibition measured at 72 hrs by trypan blue exclusion assay	[PMID: 118258]
Lymphocyte	IC₅₀	0.078 μM Compound: Prednisolone	Immunosuppressive activity in rat lymphocytes assessed as inhibition of mixed-lymphocyte reaction Immunosuppressive activity in rat lymphocytes assessed as inhibition of mixed-lymphocyte reaction	[PMID: 19179074]
MG-63	EC₅₀	19 nM Compound: 2, pred	Induction of glutamine synthase expression in human MG63 cells after overnight incubation by spectrophotometry Induction of glutamine synthase expression in human MG63 cells after overnight incubation by spectrophotometry	[PMID: 21899328]
NHDF	IC₅₀	23 nM Compound: 1	Agonist activity at human GR expressed in NHDF cells assessed as inhibition of IL-6 production by ELISA relative to Dexamethasone Agonist activity at human GR expressed in NHDF cells assessed as inhibition of IL-6 production by ELISA relative to Dexamethasone	[PMID: 21316964]
NHDF	IC₅₀	23 nM Compound: 1	Transrepression activity at glucocorticoid receptor in human NHDF cells assessed as inhibition of IL-1beta-stimulated AP1 dependent IL-6 repression by ELISA Transrepression activity at glucocorticoid receptor in human NHDF cells assessed as inhibition of IL-1beta-stimulated AP1 dependent IL-6 repression by ELISA	[PMID: 21324689]
NHDF	IC₅₀	23 nM Compound: 1	Transrepression activity at GR expressed in NHDF cells assessed as IL-1beta-mediated IL-6 transcription by ELISA Transrepression activity at GR expressed in NHDF cells assessed as IL-1beta-mediated IL-6 transcription by ELISA	[PMID: 21349714]
OVCAR-3	IC₅₀	1.1 μM Compound: 35	Cytotoxicity against human OVCAR-3 cells incubated for 72 hrs by SRB assay Cytotoxicity against human OVCAR-3 cells incubated for 72 hrs by SRB assay	[PMID: 32283298]
OVCAR-4	IC₅₀	1.1 μM Compound: 35	Cytotoxicity against human OVCAR-4 cells incubated for 72 hrs by SRB assay Cytotoxicity against human OVCAR-4 cells incubated for 72 hrs by SRB assay	[PMID: 32283298]
PBMC	IC₅₀	13 nM Compound: Pred	Antiinflammatory activity in Wistar rat PBMC assessed as inhibition of LPS-induced TNFalpha release preincubated for 45 mins followed by LPS addition after 18 hrs by ELISA Antiinflammatory activity in Wistar rat PBMC assessed as inhibition of LPS-induced TNFalpha release preincubated for 45 mins followed by LPS addition after 18 hrs by ELISA	[PMID: 27810243]
PBMC	IC₅₀	45 nM Compound: Pred	Antiinflammatory activity in human primary PBMC assessed as inhibition of LPS-induced TNFalpha release preincubated for 45 mins followed by LPS addition after 18 hrs by ELISA Antiinflammatory activity in human primary PBMC assessed as inhibition of LPS-induced TNFalpha release preincubated for 45 mins followed by LPS addition after 18 hrs by ELISA	[PMID: 27810243]
RAW264.7	IC₅₀	25.4 μM Compound: Prednisolone	Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced TNFalpha production after 4 hrs by ELISA Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced TNFalpha production after 4 hrs by ELISA	[PMID: 11000020]
T47D	IC₅₀	> 1000 nM Compound: Prednisolone	Displacement of [3H]progesterone from progesterone receptor in human T47D cells after 20 hrs by scintillation counting method Displacement of [3H]progesterone from progesterone receptor in human T47D cells after 20 hrs by scintillation counting method	[PMID: 30384041]
U-937	IC₅₀	0.033 μM Compound: Prednisolone	Antiinflammatory activity in human U937 cells assessed as inhibition of PMA/LPS-stimulated TNFalpha production after 24 hrs by ELISA Antiinflammatory activity in human U937 cells assessed as inhibition of PMA/LPS-stimulated TNFalpha production after 24 hrs by ELISA	[PMID: 32631542]
U-937	IC₅₀	0.44 μM Compound: Prednisolone	Inhibition of LPS-induced IL-1beta expression in human U-937 cells preincubated for 1 hr followed by LPS addition and measured after 24 hrs by ELISA Inhibition of LPS-induced IL-1beta expression in human U-937 cells preincubated for 1 hr followed by LPS addition and measured after 24 hrs by ELISA	[PMID: 31883489]
U-937	IC₅₀	0.64 μM Compound: Prednisolone	Inhibition of LPS-induced TNF-alpha expression in human U-937 cells preincubated for 1 hr followed by LPS addition and measured after 24 hrs by ELISA Inhibition of LPS-induced TNF-alpha expression in human U-937 cells preincubated for 1 hr followed by LPS addition and measured after 24 hrs by ELISA	[PMID: 31883489]
U-937	IC₅₀	8.2 nM Compound: pred	Antiinflammatory activity in PMA-differentiated human U937 cells assessed as inhibition of LPS-induced TNFalpha production incubated for 60 mins prior to LPS challenge measured after 24 hrs Antiinflammatory activity in PMA-differentiated human U937 cells assessed as inhibition of LPS-induced TNFalpha production incubated for 60 mins prior to LPS challenge measured after 24 hrs	[PMID: 24446728]

In Vitro

Prednisolone (0.002-10 μg/mL; 3 days) inhibits human leukocyte mitosis^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Prednisolone (5 mg/kg/day; p.o.; 6 days a week for 72 weeks) significantly prevents deaths from renal disease in mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	NZB/NZW mice, immune nephritis model^[3]
Dosage:	5 mg/kg/day
Administration:	Oral, 6 days a week for 72 weeks
Result:	Significantly lowered mortality rate and prolonged life significantly.

Molecular Weight

360.44

Formula

C₂₁H₂₈O₅

CAS No.

50-24-8

Appearance

Solid

Color

White to off-white

SMILES

C[C@@]1([C@@]2(O)C(CO)=O)[C@](CC2)([H])[C@@](CCC3=CC4=O)([H])[C@]([C@]3(C=C4)C)([H])[C@@H](O)C1

Structure Classification

Steroids

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Solvent & Solubility

In Vitro:

DMSO : 62.5 mg/mL (173.40 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 0.1 mg/mL (0.28 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7744 mL	13.8719 mL	27.7439 mL
5 mM	0.5549 mL	2.7744 mL	5.5488 mL
10 mM	0.2774 mL	1.3872 mL	2.7744 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.77 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.77 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (5.77 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.93%

Select Batch:

Data Sheet (278 KB) SDS (761 KB)

COA (252 KB) HNMR (269 KB) RP-HPLC (261 KB) LCMS (102 KB)

Handling Instructions (2659 KB)

References

[1]. Prednisolone. J Am Pharm Assoc. 1976;16(3):143-146. [Content Brief]

[2]. Musson DG, et al. Assay methodology for prednisolone, prednisolone acetate and prednisolone sodium phosphate in rabbit aqueous humor and ocular physiological solutions. J Chromatogr. 1991;565(1-2):89-102. [Content Brief]

[3]. Hahn B H, et al. Comparison of therapeutic and immunosuppressive effects of azathioprine, prednisolone and combined therapy in nzp/nzw mice. Arthritis & Rheumatism: Official Journal of the American College of Rheumatology, 1973, 16(2): 163-170.

[4]. NOWELL PC. Inhibition of human leukocyte mitosis by prednisolone in vitro. Cancer Res. 1961 Dec;21:1518-21. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.7744 mL	13.8719 mL	27.7439 mL	69.3597 mL
	5 mM	0.5549 mL	2.7744 mL	5.5488 mL	13.8719 mL
	10 mM	0.2774 mL	1.3872 mL	2.7744 mL	6.9360 mL
	15 mM	0.1850 mL	0.9248 mL	1.8496 mL	4.6240 mL
	20 mM	0.1387 mL	0.6936 mL	1.3872 mL	3.4680 mL
	25 mM	0.1110 mL	0.5549 mL	1.1098 mL	2.7744 mL
	30 mM	0.0925 mL	0.4624 mL	0.9248 mL	2.3120 mL
	40 mM	0.0694 mL	0.3468 mL	0.6936 mL	1.7340 mL
	50 mM	0.0555 mL	0.2774 mL	0.5549 mL	1.3872 mL
	60 mM	0.0462 mL	0.2312 mL	0.4624 mL	1.1560 mL
	80 mM	0.0347 mL	0.1734 mL	0.3468 mL	0.8670 mL
	100 mM	0.0277 mL	0.1387 mL	0.2774 mL	0.6936 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Prednisolone50-24-8Glucocorticoid ReceptorEndogenous Metabolitecorticosteroidglucocorticoidanti-inflammatoryorally activeInhibitorinhibitorinhibit

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