1. Fluorescent Dye
  2. Cell staining analysis
    Fluorescence Wavelength Division
  3. Mitochondria Dyes
    Ex 495-570 nm Green
    Em 495-570 nm Green
  4. Rhodamine 123

Rhodamine 123  (Synonyms: RH-123; R-22420)

Cat. No.: HY-D0816 Purity: 99.73%
COA Handling Instructions

Rhodamine 123 (RH-123; R-22420) is a fluorescent dye (λex=503 nm, λem=527 nm).

For research use only. We do not sell to patients.

Rhodamine 123 Chemical Structure

Rhodamine 123 Chemical Structure

CAS No. : 62669-70-9

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 73 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 73 In-stock
Estimated Time of Arrival: December 31
Solid
5 mg USD 66 In-stock
Estimated Time of Arrival: December 31
10 mg USD 86 In-stock
Estimated Time of Arrival: December 31
25 mg USD 119 In-stock
Estimated Time of Arrival: December 31
50 mg USD 198 In-stock
Estimated Time of Arrival: December 31
100 mg USD 330 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

Customer Review

Based on 6 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

  • Protocol

  • Purity & Documentation

  • Customer Review

Description

Rhodamine 123 (RH-123; R-22420) is a fluorescent dye (λex=503 nm, λem=527 nm).

In Vitro

The intensity of R123 fluorescence has a peak at concentration of 50 μM, and decreases to zero at higher concentrations due to self-quenching[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

380.82

Formula

C21H17ClN2O3

CAS No.
Emission (Em)
Excitation (Ex)
SMILES

O=C(C1=CC=CC=C1C2=C3C=CC(N)=CC3=[O+]C4=C2C=CC(N)=C4)OC.[Cl-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 62.5 mg/mL (164.12 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6259 mL 13.1296 mL 26.2591 mL
5 mM 0.5252 mL 2.6259 mL 5.2518 mL
10 mM 0.2626 mL 1.3130 mL 2.6259 mL
*Please refer to the solubility information to select the appropriate solvent.
Purity & Documentation

Purity: 99.73%

Kinase Assay
[1]

Measurements are made at room temperature with continuous stirring of the mitochondrial suspension using spectrophotometer equipped with a magnetic stirrer with fluorescent cation R123 as probe. Excitation and emission wavelengths are 503 nm and 527 nm, respectively. The incubation medium is the respiration buffer. R123 and sodium pyruvate are added to final concentrations of 50 nM and 10 mM, respectively. Isolated mitochondria maintain a steady membrane potential (±5%) throughout the duration of the recording[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

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Rhodamine 123
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