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  4. Rhodamine 123

Rhodamine 123  (Synonyms: RH-123; R-22420)

Cat. No.: HY-D0816 Purity: 99.47%
COA
SDS
Handling Instructions Technical Support

Rhodamine dyes are membrane-permeable cationic fluorescent probes that specifically recognize mitochondrial membrane potentials, thereby attaching to mitochondria and producing bright fluorescence, and at certain concentrations, rhodamine dyes have low toxicity to cells, so they are commonly used to detect mitochondria in animal cells, plant cells, and microorganisms.

For research use only. We do not sell to patients.

CAS No. : 62669-70-9

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
5 mg In-stock
10 mg In-stock
25 mg In-stock
50 mg In-stock
100 mg In-stock
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 27 publication(s) in Google Scholar

Other Forms of Rhodamine 123:

Rhodamine 123 Related Antibodies

Top Publications Citing Use of Products

    Rhodamine 123 purchased from MedChemExpress. Usage Cited in: Cancer Res. 2025 Sep 3.  [Abstract]

    FACS detection of mitochondrial mass using MitoTracker Green and assessment of mitochondrial membrane potential using Rhodamine-123 (50 μM; 30 min).

    Rhodamine 123 purchased from MedChemExpress. Usage Cited in: J Control Release. 2025 Jan 8:379:45-58.  [Abstract]

    Evaluation of P-gp function using Rhod-123 efflux assay. The fluorescent images for the uptake of Rhod-123 (3 μM) (red channel, Ex/Em = 507/529 nm) by A549-DDP and A549 at 0.5, 1, 2, and 4 h were obtained by the HCA System.

    Rhodamine 123 purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2025 Sep 25:102371.  [Abstract]

    Mitochondrial membrane potential assessment in A375 cells after 1 h treatments, using Rhodamine 123 (250 nM), with MitoTracker Red (mitochondrial marker). Scale bars, 20 μm.

    Rhodamine 123 purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2025 Nov 28;23(1):514.  [Abstract]

    Rhodamine 123 (5 μM; 30 min) staining and BODIPY 493/503 staining for analyzing mitochondrial membrane potential and lipid droplets.

    Rhodamine 123 purchased from MedChemExpress. Usage Cited in: J Adv Res. 2024 Sep 11:S2090-1232(24)00390-4.  [Abstract]

    OE-LAMC2-Miapaca‐2-GR and OE-LAMC2-Capan‐2-GR cells were pretreated with or without Rh2 (20 μM) for 24 hours, and then incubated in the dark with 10 μM Rhodamine 123 for 30 minutes.

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Rhodamine dyes are membrane-permeable cationic fluorescent probes that specifically recognize mitochondrial membrane potentials, thereby attaching to mitochondria and producing bright fluorescence, and at certain concentrations, rhodamine dyes have low toxicity to cells, so they are commonly used to detect mitochondria in animal cells, plant cells, and microorganisms[1].

    In Vitro

    Guide (The following is the experimental plan we recommend. This plan serves only as a reference guide. The specific operations should be adjusted according to your actual needs.)
    1. Preparation of Rhodamine 123 Solution
    1.1 Preparation of Reserve Solution
    Dissolve Rhodamine 123 in DMSO to obtain a 5 mM reserve solution.
    1.2 Preparation of Rhodamine 123 Working Solution
    Dilute the reserve solution with serum-free cell culture medium or PBS to obtain a working solution of 1 - 20 μM.
    Note: Please adjust the concentration of Rhodamine 123 working solution according to the actual situation.
    2. Cell Staining
    2.1 Suspended Cells (6-well plate)
    a. Centrifuge at 1000g for 3-5 minutes at 4℃, discard the supernatant. Wash twice with PBS, each time for 5 minutes. The cell density is 1×106/mL.
    b. Add 1 mL of the working solution, incubate at room temperature for 5 - 30 minutes.
    c. Centrifuge at 400g for 3 - 4 minutes, discard the supernatant.
    d. Wash twice with PBS, each time for 5 minutes.
    e. Resuspend the cells with serum-free cell culture medium or PBS. Observe with a fluorescence microscope or flow cytometer.
    2.2 Attached Cells
    a. Cultivate attached cells on a sterile cover glass.
    b. Remove the cover glass from the culture medium and aspirate the excess medium.
    c. Add 100 μL of the working solution, gently shake to completely cover the cells, then incubate at room temperature for 30 - 60 minutes.
    d. Wash twice with the culture medium, each time for 5 minutes. Observe with a fluorescence microscope or flow cytometer.
    Note: If using a flow cytometer for detection, the cells need to be resuspended before staining.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Rhodamine 123 Related Antibodies
    Molecular Weight

    380.82

    Formula

    C21H17ClN2O3
    CAS No.
    Appearance

    Solid

    Color

    Brown to red

    Emission (Em)

    529
    Excitation (Ex)

    507
    SMILES

    O=C(C1=CC=CC=C1C2=C3C=CC(N)=CC3=[O+]C4=C2C=CC(N)=C4)OC.[Cl-]
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 41.67 mg/mL (109.42 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.6259 mL 13.1296 mL 26.2591 mL
    5 mM 0.5252 mL 2.6259 mL 5.2518 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.55%

    SDS (393 KB)

    COA (253 KB) HNMR (256 KB) RP-HPLC (259 KB) MS (203 KB)

    Handling Instructions (2659 KB)
    References
    Kinase Assay
    [1]

    Measurements are made at room temperature with continuous stirring of the mitochondrial suspension using spectrophotometer equipped with a magnetic stirrer with fluorescent cation R123 as probe. Excitation and emission wavelengths are 503 nm and 527 nm, respectively. The incubation medium is the respiration buffer. R123 and sodium pyruvate are added to final concentrations of 50 nM and 10 mM, respectively. Isolated mitochondria maintain a steady membrane potential (±5%) throughout the duration of the recording[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.6259 mL 13.1296 mL 26.2591 mL 65.6478 mL
    5 mM 0.5252 mL 2.6259 mL 5.2518 mL 13.1296 mL
    10 mM 0.2626 mL 1.3130 mL 2.6259 mL 6.5648 mL
    15 mM 0.1751 mL 0.8753 mL 1.7506 mL 4.3765 mL
    20 mM 0.1313 mL 0.6565 mL 1.3130 mL 3.2824 mL
    25 mM 0.1050 mL 0.5252 mL 1.0504 mL 2.6259 mL
    30 mM 0.0875 mL 0.4377 mL 0.8753 mL 2.1883 mL
    40 mM 0.0656 mL 0.3282 mL 0.6565 mL 1.6412 mL
    50 mM 0.0525 mL 0.2626 mL 0.5252 mL 1.3130 mL
    60 mM 0.0438 mL 0.2188 mL 0.4377 mL 1.0941 mL
    80 mM 0.0328 mL 0.1641 mL 0.3282 mL 0.8206 mL
    100 mM 0.0263 mL 0.1313 mL 0.2626 mL 0.6565 mL
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    Rhodamine 123 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Rhodamine 12362669-70-9RH-123 R-22420Rhodamine123Rhodamine-123RH123RH 123R22420R 22420R-22420Fluorescent DyeInhibitorinhibitorinhibit

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