1. Metabolic Enzyme/Protease
  2. Phosphodiesterase (PDE)
  3. Vesnarinone

Vesnarinone  (Synonyms: OPC-8212)

Cat. No.: HY-15297 Purity: 98.07%
COA Handling Instructions

Vesnarinone (OPC-8212) is an orally active phosphodiesterase 3 (PDE3) inhibitor. Vesnarinone can increase in calcium flux and decrease in potassium flux. Vesnarinone shows dose-dependent positive inotropic activity. Vesnarinone can be used in heart failure research.

For research use only. We do not sell to patients.

Vesnarinone Chemical Structure

Vesnarinone Chemical Structure

CAS No. : 81840-15-5

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10 mM * 1 mL in DMSO USD 87 In-stock
Estimated Time of Arrival: December 31
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ready for reconstitution
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10 mg USD 150 In-stock
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25 mg USD 250 In-stock
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50 mg USD 450 In-stock
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100 mg USD 750 In-stock
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Based on 1 publication(s) in Google Scholar

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Description

Vesnarinone (OPC-8212) is an orally active phosphodiesterase 3 (PDE3) inhibitor. Vesnarinone can increase in calcium flux and decrease in potassium flux. Vesnarinone shows dose-dependent positive inotropic activity. Vesnarinone can be used in heart failure research[1][2][3][4].

In Vitro

Vesnarinone (60 and 100 μg/mL; 48 h) inhibits the cell growth in a dose-dependent manner[3].
Vesnarinone (60 μg/mL; 48 h) induces G1 arrest and apoptosis[3].
Vesnarinone (60 μg/mL; 0, 12, 24, and 48 h) treatment increases p21-mRNA expression and decreases p21 protein slightly[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[3]

Cell Line: OSC3, OSC4, and OSC5 cells
Concentration: 60 μg/mL and 100 μg/mL
Incubation Time: 48 hours
Result: Observed more than 50% inhibition in cells treated with 100 μg/mL, and reduced 30±40% the production of MTT formazan in cells treated with 60 μg/mL.

Apoptosis Analysis[3]

Cell Line: OSC3, OSC4, and OSC5 cells
Concentration: 60 μg/mL
Incubation Time: 48 hours
Result: Decreased the tumor cell population in the S phase and increased in the G1 phase.

Western Blot Analysis[3]

Cell Line: OSC3, OSC4, and OSC5 cells
Concentration: 60 μg/mL
Incubation Time: 0, 12, 24, and 48 hours
Result: Increased the expression of p21-mRNA after 12±24 h vesnarinone treatment.
Decreased p21 protein slightly after 24 h treatment in OSC4.
In Vivo

Vesnarinone (oral gavage; 300 mg/kg; once daily; 6 w) binds covalently to rat liver in vivo[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female Lewis rats (200 g)[4]
Dosage: 300 mg/kg
Administration: Oral gavage; 300 mg/kg; once daily; 6 weeks
Result: Observed covalently modified liver proteins.
Clinical Trial
Molecular Weight

395.45

Formula

C22H25N3O4

CAS No.
SMILES

O=C1NC2=C(C=C(N3CCN(C(C4=CC=C(OC)C(OC)=C4)=O)CC3)C=C2)CC1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 16.67 mg/mL (42.15 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5288 mL 12.6438 mL 25.2876 mL
5 mM 0.5058 mL 2.5288 mL 5.0575 mL
10 mM 0.2529 mL 1.2644 mL 2.5288 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 1.67 mg/mL (4.22 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 1.67 mg/mL (4.22 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 1.67 mg/mL (4.22 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Vesnarinone
Cat. No.:
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