Vitamin K1
Based on 8 publication(s) in Google Scholar
Vitamin K1 a naturally occurring vitamin required for blood coagulation and bone and vascular metabolism.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 98.60%
- No. CAS: 84-80-0
- Fòrmula: C31H46O2
- Peso molecular:450.70
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Almacenamiento:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Vitamin K1
More- Cell Rep Med. 2025 Oct 21;6(10):102410. [Abstract]
- J Pharm Anal. 2025 May;15(5):101092. [Abstract]
- Mater Today Bio. 2025 Oct 1:35:102378. [Abstract]
- Acta Pharmacol Sin. 2025 Jun 23. [Abstract]
- J Med Chem. 2021 Oct 14;64(19):14344-14357. [Abstract]
- Int J Mol Sci. 2023 Apr 28;24(9):8012. [Abstract]
- Neurochem Res. 2025 Nov 26;51(1):2. [Abstract]
- bioRxiv. 2025 Feb 19:2025.02.18.638829. [Abstract]
Customer Validation & Images
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Cell Proliferation/Viability Assay
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Cell Imaging/Staining
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WB
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RT-PCR
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IF
Actividad biológica
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Human Endogenous Metabolite |
Phylloquinone (Vitamin K1) is a prenylated naphthoquinone that is synthesized exclusively by plants, green algae, and some species of cyanobacteria, where it serves as a vital electron carrier in photosystem I and as an electron acceptor for the formation of protein disulfide bonds. In humans and other vertebrates, phylloquinone plays the role of a vitamin (vitamin K1) that is required for blood coagulation and bone and vascular metabolism. Phylloquinone from green leafy vegetables and vegetable oil represents the major dietary source of vitamin K for humans[1]. Vitamin K1 treatment causes a significant antiproliferative effect and induces apoptosis in Caco-2, HT-29, and SW480 cell lines, with the involvement of the MAPK pathway. A concomitant and significant decrease in the polyamine biosynthesis occurr[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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No. CAS 84-80-0
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Appearance Liquid (Density: 0.984 g/cm3)
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Peso molecular 450.70
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Fòrmula C31H46O2
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Color Light yellow to yellow
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SMILES
O=C1C(C)=C(C/C=C(C)/CCC[C@H](C)CCC[C@H](C)CCCC(C)C)C(C2=C1C=CC=C2)=O
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Synonyms
Phylloquinone; Phytomenadione
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Structure Classification
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (8)
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Journal Impact Factor
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Most Recent
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Cell Rep Med
Intestinal Lachnospiraceae bacterium-derived propionate inhibits the progression of clear cell renal cell carcinoma. [Abstract]2025 Oct 21;6(10):102410. PMID: 41067230 -
J Pharm Anal
Screen of FDA-approved drug library identifies vitamin K as anti-ferroptotic drug for osteoarthritis therapy through Gas6. [Abstract]2025 May;15(5):101092. PMID: 40496065
Vitamin K1 purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 May;15(5):101092. [Abstract]
Cell viability of chondrocytes treated with ferroptosis inducers ((1S,3R)-RSL3 (RSL3; 0.5 μM), vitamin K1 (VK1,10 μM) and ferroptosis inhibitor Ferrostatin-1 (Fer-1, 1 μM) for 24 h was detected by Cell Counting Kit-8 (CCK-8).
Vitamin K1 purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 May;15(5):101092. [Abstract]
Bright-field and fluorescent images of live/dead staining of chondrocytes treated with ferroptosis inducers (1S,3R)-RSL3 (RSL3; 0.5 μM), vitamin K1 (VK1;10 μM) and ferroptosis inhibitor Ferrostatin-1 (Fer-1; 1 μM) for 24 h. Propidium iodide (PI) stained (Red) for dead cells, and Calcein-AM (Green) stained for live cells.
Vitamin K1 purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 May;15(5):101092. [Abstract]
The protein levels of MMP13, ADAMTS4, COL II and Aggrecan in chondrocytes treated with ferroptosis inducers erastin (10 μM), vitamin K1 (VK1;10 μM) and ferroptosis inhibitor Ferrostatin-1 (Fer-1; 1 μM) for 24 h were determined by Western blotting assay
Vitamin K1 purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 May;15(5):101092. [Abstract]
The mRNA levels of catabolic enzymes (Mmp13 and Adamts4) and matrix components (Col2a1 and Acan) in chondrocytes treated with ferroptosis inducers erastin (10 μM), vitamin K1 (VK1; 10 μM) and ferroptosis inhibitor Ferrostatin-1 (Fer-1; 1 μM) for 24 h were determined by RT-qPCR.
Vitamin K1 purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 May;15(5):101092. [Abstract]
Immunofluorescence of MMP13, ADAMTS4, COL II and Aggrecan in chondrocytes treated with ferroptosis inducers erastin (10 μM), vitamin K1 (VK1; 10 μM) and ferroptosis inhibitor Ferrostatin-1 (Fer-1; 1 μM) for 24 h.
Vitamin K1 purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 May;15(5):101092. [Abstract]
Representative images of IHC of matrix metalloproteinase 13 (MMP13), collagen type II (COL II), GPX4 and Gas6 in cartilage sections of mice injected intraarticularly with VK1 (1 mg/kg, DMM + VK1 + AAV-NC and DMM + VK1 + AAV-shGas6 groups) or vehicle (Sham + AAV-NC groups) twice a week for two weeks.
Vitamin K1 purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 May;15(5):101092. [Abstract]
Representative images of H&E, safranin O fast green and toluidine blue staining of cartilage sections of mice injected intraarticularly with VK1 (1 mg/kg, DMM + VK1 + AAV-NC and DMM + VK1 + AAV-shGas6 groups), Ferrostatin-1 (Fer-1; 0.1 mg/kg, DMM + Fer-1 + AAV-NC group) or vehicle (Sham + AAV-NC and DMM + AAV-NC groups) twice a week for two weeks.
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Mater Today Bio
Selective targeting of coagulation factor X Gla domain by negatively charged gold nanoparticles: a novel method for controlled antithrombotic therapy. [Abstract]2025 Oct 1:35:102378. PMID: 41111485 -
Acta Pharmacol Sin
Ginsenoside (20)S-APPT induces ferroptosis in hepatocellular carcinoma and cholangiocarcinoma by targeting FSP1. [Abstract]2025 Jun 23. PMID: 40550962 -
J Med Chem
Repositioning of the Anthelmintic Drugs Bithionol and Triclabendazole as Transthyretin Amyloidogenesis Inhibitors. [Abstract]2021 Oct 14;64(19):14344-14357. PMID: 34547896 -
Int J Mol Sci
Transcriptomic and Functional Analyses Reveal the Different Roles of Vitamins C, E, and K in Regulating Viral Infections in Maize. [Abstract]2023 Apr 28;24(9):8012. PMID: 37175719 -
Neurochem Res
Phylloquinone Attenuates Oxygen-Glucose Deprivation-induced Neuronal Injury by Inhibiting Ferroptosis via the xCT/GPX4 Pathway. [Abstract]2025 Nov 26;51(1):2. PMID: 41296108 -
bioRxiv
Structural and Functional Insights into GGCX-FIX Interaction: Implications for Vitamin K-Dependent Bleeding Disorders. [Abstract]2025 Feb 19:2025.02.18.638829. PMID: 40027771
Solvente y solubilidad
Ethanol : ≥ 50 mg/mL (110.94 mM)
DMSO : 6.67 mg/mL (14.80 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.55 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (5.55 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.55 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocolo
Caco-2, HT-29, and SW480 cells are treated with increasing concentrations of vitamin K1 (10, 50, 100, and 200 μM) for 24 h, 48 h, and 72 h. MTT is added to each dish and incubated for 2 h at 37°C. At the end of the incubation period, the medium is removed. The plate is read at 570 nM[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Rats: For inducing full-thickness wound in rats, the excisional wound model is used. Five groups consisting of 8 rats each are used. Vitamin K cream (1% and 2%, w/w) is prepared in eucerin base and applied on the wound once a day until complete healing had occurred. Healing is defined by decreased wound margin (wound contraction), re-epithelialization, tensile strength and hydroxyproline content. Histopathological examination is also done[5].
Mice: Four-week-old C57BL/6J male mice are fed a 10% fat normal diet group or a 45% kcal high-fat diet group, with or without 200 mg/1000 g vitamin K1 (Normal diet + K1, high-fat diet + K1) and 200 mg/1000 g vitamin K2 (Normal diet + K2, high-fat diet + K2) for 12 weeks[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Pureza y Documentación
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Ficha de datos (275 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Basset GJ, et al. Phylloquinone (Vitamin K1): Occurrence, Biosynthesis and Functions. Mini Rev Med Chem. 2016 Jun 22. [Content Brief]
[2]. Orlando A, et al. Vitamin K1 exerts antiproliferative effects and induces apoptosis in three differently graded human colon cancer cell lines. Biomed Res Int. 2015;2015:296721. [Content Brief]
[3]. Ibarrola-Jurado N, et al. Dietary phylloquinone intake and risk of type 2 diabetes in elderly subjects at high risk of cardiovascular disease. Am J Clin Nutr. 2012 Nov;96(5):1113-8. [Content Brief]
[4]. Kim M, et al. Vitamin K1 (phylloquinone) and K2 (menaquinone-4) supplementation improves bone formation in a high-fat diet-induced obese mice. J Clin Biochem Nutr. 2013 Sep;53(2):108-13. [Content Brief]
[5]. Hemmati AA, et al. Topical vitamin K1 promotes repair of full thickness wound in rat. Indian J Pharmacol. 2014 Jul-Aug;46(4):409-12. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO / Ethanol | 1 mM | 2.2188 mL | 11.0939 mL | 22.1877 mL | 55.4693 mL |
| 5 mM | 0.4438 mL | 2.2188 mL | 4.4375 mL | 11.0939 mL | |
| 10 mM | 0.2219 mL | 1.1094 mL | 2.2188 mL | 5.5469 mL | |
| Ethanol | 15 mM | 0.1479 mL | 0.7396 mL | 1.4792 mL | 3.6980 mL |
| 20 mM | 0.1109 mL | 0.5547 mL | 1.1094 mL | 2.7735 mL | |
| 25 mM | 0.0888 mL | 0.4438 mL | 0.8875 mL | 2.2188 mL | |
| 30 mM | 0.0740 mL | 0.3698 mL | 0.7396 mL | 1.8490 mL | |
| 40 mM | 0.0555 mL | 0.2773 mL | 0.5547 mL | 1.3867 mL | |
| 50 mM | 0.0444 mL | 0.2219 mL | 0.4438 mL | 1.1094 mL | |
| 60 mM | 0.0370 mL | 0.1849 mL | 0.3698 mL | 0.9245 mL | |
| 80 mM | 0.0277 mL | 0.1387 mL | 0.2773 mL | 0.6934 mL | |
| 100 mM | 0.0222 mL | 0.1109 mL | 0.2219 mL | 0.5547 mL |
Vitamin K1 Related Classifications
HY-N0684 Related Classifications
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