Cat. No.: HY-N0684

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Vitamin K1

(Synonyms: Phylloquinone; Phytomenadione)

8 Cited Publications

Customer Review

Based on 8 publication(s) in Google Scholar

Vitamin K1 a naturally occurring vitamin required for blood coagulation and bone and vascular metabolism.

For research use only. We do not sell to patients.

Purity: 98.60%
CAS No.: 84-80-0
Formula: C₃₁H₄₆O₂
Molecular Weight:450.70

Storage:

Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Vitamin K1 Chemical Structure

CAS No.: 84-80-0

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (8)
Solvent & Solubility
- In Vitro
- In Vivo
Protocol
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Vitamin K1 a naturally occurring vitamin required for blood coagulation and bone and vascular metabolism.

IC₅₀ & Target

Human Endogenous Metabolite

In Vitro

Phylloquinone (Vitamin K1) is a prenylated naphthoquinone that is synthesized exclusively by plants, green algae, and some species of cyanobacteria, where it serves as a vital electron carrier in photosystem I and as an electron acceptor for the formation of protein disulfide bonds. In humans and other vertebrates, phylloquinone plays the role of a vitamin (vitamin K1) that is required for blood coagulation and bone and vascular metabolism. Phylloquinone from green leafy vegetables and vegetable oil represents the major dietary source of vitamin K for humans^[1]. Vitamin K1 treatment causes a significant antiproliferative effect and induces apoptosis in Caco-2, HT-29, and SW480 cell lines, with the involvement of the MAPK pathway. A concomitant and significant decrease in the polyamine biosynthesis occurr^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Subjects who increase their dietary intake of vitamin K during the follow-up had a 51% reduced risk of incident diabetes compared with subjects who decrease or does not change the amount of phylloquinone intake^[3]. Vitamin K supplementation reverses the high fat diet induced bone deterioration by modulating osteoblast and osteoclast activities and prevent bone loss in a high-fat diet-induced obese mice^[4]. Application of vitamin K1 to the skin has been used for suppression of pigmentation and resolution of bruising. The effects produced by the topical vitamin K1 shows significant healing when compared with control group in parameters such as wound contraction, epithelialization period, hydroxyproline content and tensile strength^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 84-80-0
Appearance Liquid (Density: 0.984 g/cm³)
Molecular Weight 450.70
Formula C₃₁H₄₆O₂
Color Light yellow to yellow
SMILES

O=C1C(C)=C(C/C=C(C)/CCC[C@H](C)CCC[C@H](C)CCCC(C)C)C(C2=C1C=CC=C2)=O
Synonyms

Phylloquinone; Phytomenadione
Structure Classification
- Quinones
- Naphthalene Quinones
Initial Source
- Plants
- other families
- Endogenous metabolite
- Human Gut Microbiota Metabolites
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (8)

All Cell Proliferation/Viability AssayCell Imaging/StainingWBRT-PCRIFIHCHistological Imaging/Staining

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Journal Impact Factor

Cell Rep Med

Intestinal Lachnospiraceae bacterium-derived propionate inhibits the progression of clear cell renal cell carcinoma. [Abstract]

2025 Oct 21;6(10):102410. PMID: 41067230
Mater Today Bio

Selective targeting of coagulation factor X Gla domain by negatively charged gold nanoparticles: a novel method for controlled antithrombotic therapy. [Abstract]

2025 Oct 1:35:102378. PMID: 41111485
J Pharm Anal

Screen of FDA-approved drug library identifies vitamin K as anti-ferroptotic drug for osteoarthritis therapy through Gas6. [Abstract]

2025 May;15(5):101092. PMID: 40496065

Vitamin K1 purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 May;15(5):101092. [Abstract]

Cell viability of chondrocytes treated with ferroptosis inducers ((1S,3R)-RSL3 (RSL3; 0.5 μM), vitamin K1 (VK1,10 μM) and ferroptosis inhibitor Ferrostatin-1 (Fer-1, 1 μM) for 24 h was detected by Cell Counting Kit-8 (CCK-8).

Vitamin K1 purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 May;15(5):101092. [Abstract]

Bright-field and fluorescent images of live/dead staining of chondrocytes treated with ferroptosis inducers (1S,3R)-RSL3 (RSL3; 0.5 μM), vitamin K1 (VK1;10 μM) and ferroptosis inhibitor Ferrostatin-1 (Fer-1; 1 μM) for 24 h. Propidium iodide (PI) stained (Red) for dead cells, and Calcein-AM (Green) stained for live cells.

Vitamin K1 purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 May;15(5):101092. [Abstract]

The protein levels of MMP13, ADAMTS4, COL II and Aggrecan in chondrocytes treated with ferroptosis inducers erastin (10 μM), vitamin K1 (VK1;10 μM) and ferroptosis inhibitor Ferrostatin-1 (Fer-1; 1 μM) for 24 h were determined by Western blotting assay

Vitamin K1 purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 May;15(5):101092. [Abstract]

The mRNA levels of catabolic enzymes (Mmp13 and Adamts4) and matrix components (Col2a1 and Acan) in chondrocytes treated with ferroptosis inducers erastin (10 μM), vitamin K1 (VK1; 10 μM) and ferroptosis inhibitor Ferrostatin-1 (Fer-1; 1 μM) for 24 h were determined by RT-qPCR.

Vitamin K1 purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 May;15(5):101092. [Abstract]

Immunofluorescence of MMP13, ADAMTS4, COL II and Aggrecan in chondrocytes treated with ferroptosis inducers erastin (10 μM), vitamin K1 (VK1; 10 μM) and ferroptosis inhibitor Ferrostatin-1 (Fer-1; 1 μM) for 24 h.

Vitamin K1 purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 May;15(5):101092. [Abstract]

Representative images of IHC of matrix metalloproteinase 13 (MMP13), collagen type II (COL II), GPX4 and Gas6 in cartilage sections of mice injected intraarticularly with VK1 (1 mg/kg, DMM + VK1 + AAV-NC and DMM + VK1 + AAV-shGas6 groups) or vehicle (Sham + AAV-NC groups) twice a week for two weeks.

Vitamin K1 purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 May;15(5):101092. [Abstract]

Representative images of H&E, safranin O fast green and toluidine blue staining of cartilage sections of mice injected intraarticularly with VK1 (1 mg/kg, DMM + VK1 + AAV-NC and DMM + VK1 + AAV-shGas6 groups), Ferrostatin-1 (Fer-1; 0.1 mg/kg, DMM + Fer-1 + AAV-NC group) or vehicle (Sham + AAV-NC and DMM + AAV-NC groups) twice a week for two weeks.
Acta Pharmacol Sin

Ginsenoside (20)S-APPT induces ferroptosis in hepatocellular carcinoma and cholangiocarcinoma by targeting FSP1. [Abstract]

2025 Jun 23. PMID: 40550962
J Med Chem

Repositioning of the Anthelmintic Drugs Bithionol and Triclabendazole as Transthyretin Amyloidogenesis Inhibitors. [Abstract]

2021 Oct 14;64(19):14344-14357. PMID: 34547896
Int J Mol Sci

Transcriptomic and Functional Analyses Reveal the Different Roles of Vitamins C, E, and K in Regulating Viral Infections in Maize. [Abstract]

2023 Apr 28;24(9):8012. PMID: 37175719
Neurochem Res

Phylloquinone Attenuates Oxygen-Glucose Deprivation-induced Neuronal Injury by Inhibiting Ferroptosis via the xCT/GPX4 Pathway. [Abstract]

2025 Nov 26;51(1):2. PMID: 41296108
bioRxiv

Structural and Functional Insights into GGCX-FIX Interaction: Implications for Vitamin K-Dependent Bleeding Disorders. [Abstract]

2025 Feb 19:2025.02.18.638829. PMID: 40027771

Solvent & Solubility

In Vitro:

Ethanol : ≥ 50 mg/mL (110.94 mM)

DMSO : 6.67 mg/mL (14.80 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.2188 mL

11.0939 mL

22.1877 mL

5 mM

0.4438 mL

2.2188 mL

4.4375 mL

10 mM

0.2219 mL

1.1094 mL

2.2188 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (5.55 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)

Solubility: 2.5 mg/mL (5.55 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% EtOH 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (5.55 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protocol

Cell Assay^[2]

Caco-2, HT-29, and SW480 cells are treated with increasing concentrations of vitamin K1 (10, 50, 100, and 200 μM) for 24 h, 48 h, and 72 h. MTT is added to each dish and incubated for 2 h at 37°C. At the end of the incubation period, the medium is removed. The plate is read at 570 nM^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration^[4]^[5]

Rats: For inducing full-thickness wound in rats, the excisional wound model is used. Five groups consisting of 8 rats each are used. Vitamin K cream (1% and 2%, w/w) is prepared in eucerin base and applied on the wound once a day until complete healing had occurred. Healing is defined by decreased wound margin (wound contraction), re-epithelialization, tensile strength and hydroxyproline content. Histopathological examination is also done^[5].

Mice: Four-week-old C57BL/6J male mice are fed a 10% fat normal diet group or a 45% kcal high-fat diet group, with or without 200 mg/1000 g vitamin K1 (Normal diet + K1, high-fat diet + K1) and 200 mg/1000 g vitamin K2 (Normal diet + K2, high-fat diet + K2) for 12 weeks^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Basset GJ, et al. Phylloquinone (Vitamin K1): Occurrence, Biosynthesis and Functions. Mini Rev Med Chem. 2016 Jun 22. [Content Brief]

[2]. Orlando A, et al. Vitamin K1 exerts antiproliferative effects and induces apoptosis in three differently graded human colon cancer cell lines. Biomed Res Int. 2015;2015:296721. [Content Brief]

[3]. Ibarrola-Jurado N, et al. Dietary phylloquinone intake and risk of type 2 diabetes in elderly subjects at high risk of cardiovascular disease. Am J Clin Nutr. 2012 Nov;96(5):1113-8. [Content Brief]

[4]. Kim M, et al. Vitamin K1 (phylloquinone) and K2 (menaquinone-4) supplementation improves bone formation in a high-fat diet-induced obese mice. J Clin Biochem Nutr. 2013 Sep;53(2):108-13. [Content Brief]

[5]. Hemmati AA, et al. Topical vitamin K1 promotes repair of full thickness wound in rat. Indian J Pharmacol. 2014 Jul-Aug;46(4):409-12. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO / Ethanol	1 mM	2.2188 mL	11.0939 mL	22.1877 mL	55.4693 mL
	5 mM	0.4438 mL	2.2188 mL	4.4375 mL	11.0939 mL
	10 mM	0.2219 mL	1.1094 mL	2.2188 mL	5.5469 mL
Ethanol	15 mM	0.1479 mL	0.7396 mL	1.4792 mL	3.6980 mL
	20 mM	0.1109 mL	0.5547 mL	1.1094 mL	2.7735 mL
	25 mM	0.0888 mL	0.4438 mL	0.8875 mL	2.2188 mL
	30 mM	0.0740 mL	0.3698 mL	0.7396 mL	1.8490 mL
	40 mM	0.0555 mL	0.2773 mL	0.5547 mL	1.3867 mL
	50 mM	0.0444 mL	0.2219 mL	0.4438 mL	1.1094 mL
	60 mM	0.0370 mL	0.1849 mL	0.3698 mL	0.9245 mL
	80 mM	0.0277 mL	0.1387 mL	0.2773 mL	0.6934 mL
	100 mM	0.0222 mL	0.1109 mL	0.2219 mL	0.5547 mL

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Vitamin K1

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Publications Citing Use of MedChemExpress (MCE) Vitamin K1

Biological Activity

Chemical Information

Publications (8)

Solvent & Solubility

Protocol

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Vitamin K1 Related Classifications

HY-N0684 Related Classifications