1. GPCR/G Protein Metabolic Enzyme/Protease
  2. Adenosine Receptor Endogenous Metabolite
  3. Adenosine-2'-monophosphate

Adenosine-2'-monophosphate  (Synonyms: 2'-AMP; Adenosine 2'-phosphate; AMP 2'-phosphate)

Cat. No.: HY-124151 Purity: ≥98.0%
COA Handling Instructions

Adenosine-2'-monophosphate (2'-AMP) is converted by extracellular 2’,3'-CAMP. Adenosine-2'-monophosphate is further metabolized to extracellular adenosine (a mechanism called the extracellular 2’,3’-cAMP-adenosine pathway). Adenosine-2'-monophosphate inhibits LPS-induced TNF-α and CXCL10 production via A2A receptor activation.

For research use only. We do not sell to patients.

Adenosine-2'-monophosphate Chemical Structure

Adenosine-2'-monophosphate Chemical Structure

CAS No. : 130-49-4

Size Price Stock Quantity
5 mg USD 109 In-stock
10 mg USD 170 In-stock
25 mg USD 320 In-stock
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Based on 1 publication(s) in Google Scholar

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Description

Adenosine-2'-monophosphate (2'-AMP) is converted by extracellular 2’,3'-CAMP. Adenosine-2'-monophosphate is further metabolized to extracellular adenosine (a mechanism called the extracellular 2’,3’-cAMP-adenosine pathway). Adenosine-2'-monophosphate inhibits LPS-induced TNF-α and CXCL10 production via A2A receptor activation[1][2].

IC50 & Target

A2A adenosine receptor

 

A2B adenosine receptor

 

Human Endogenous Metabolite

 

In Vitro

Adenosine-2'-monophosphate (2'-AMP) (0-100 µM; daily for 4 days) inhibits proliferation of preglomerular vascular smooth muscle cells and glomerular mesangial cells via A2B receptors[1].
Adenosine-2'-monophosphate (30 μM; 24 hours) inhibits LPS induced (100 ng/ml) TNF-α and CXCL10 production in primary murine microglia[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

347.22

Formula

C10H14N5O7P

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=[P](O)(O)O[C@H]([C@@H]1O)[C@@](N2C3=NC=NC(N)=C3N=C2)([H])O[C@@H]1CO

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 62.5 mg/mL (180.00 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 50 mg/mL (144.00 mM; ultrasonic and adjust pH to 7 with NaOH)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8800 mL 14.4001 mL 28.8002 mL
5 mM 0.5760 mL 2.8800 mL 5.7600 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (7.20 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (7.20 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: ≥98.0%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 2.8800 mL 14.4001 mL 28.8002 mL 72.0005 mL
5 mM 0.5760 mL 2.8800 mL 5.7600 mL 14.4001 mL
10 mM 0.2880 mL 1.4400 mL 2.8800 mL 7.2000 mL
15 mM 0.1920 mL 0.9600 mL 1.9200 mL 4.8000 mL
20 mM 0.1440 mL 0.7200 mL 1.4400 mL 3.6000 mL
25 mM 0.1152 mL 0.5760 mL 1.1520 mL 2.8800 mL
30 mM 0.0960 mL 0.4800 mL 0.9600 mL 2.4000 mL
40 mM 0.0720 mL 0.3600 mL 0.7200 mL 1.8000 mL
50 mM 0.0576 mL 0.2880 mL 0.5760 mL 1.4400 mL
60 mM 0.0480 mL 0.2400 mL 0.4800 mL 1.2000 mL
80 mM 0.0360 mL 0.1800 mL 0.3600 mL 0.9000 mL
100 mM 0.0288 mL 0.1440 mL 0.2880 mL 0.7200 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Adenosine-2'-monophosphate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Adenosine-2'-monophosphate
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