Aldosterone
Based on 10 publication(s) in Google Scholar
Aldosterone is the primary mineralocorticoid. Aldosterone is a steroid hormone, and it is synthesized and secreted in response to renin-angiotensin system activation (RAS) or high dietary potassium by the zona glomerulosa (ZG) of the adrenal cortex. Aldosterone activity is dependent by the binding and activation of the cytoplasmic/nuclear mineralocorticoid receptor (MR) at cellular level.
For research use only. We do not sell to patients.
- Purity: 99.56%
- CAS No.: 52-39-1
- Formula: C21H28O5
- Molecular Weight:360.44
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Aldosterone
More- Science. 2026 Feb 5;391(6785):eadz4075. [Abstract]
- Nat Chem Biol. 2022 Nov;18(11):1196-1203. [Abstract]
- Acta Pharmacol Sin. 2022 Sep;43(9):2429-2438. [Abstract]
- J Med Chem. 2026 Jan 22;69(2):1507-1529. [Abstract]
- J Med Chem. 2022 Dec 8;65(23):15710-15724. [Abstract]
- Eur J Med Chem. 2022 Jul 5:237:114382. [Abstract]
- Cell Signal. 2024 Aug 13:111346. [Abstract]
- Int J Endocrinol. 2021 Jun 18:2021:5575927. [Abstract]
- Int Urol Nephrol. 2025 Feb;57(2):613-623. [Abstract]
- Gene Rep. 2025 Oct 4.
Customer Validation & Images
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Bio/Physico-chemical Assay
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ELISA
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RT-PCR
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WB
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WB
All Endogenous Metabolite Isoforms
More
Biological Activity
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Human Endogenous Metabolite |
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Cell Line
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Type | Value | Description | References |
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| COS-7 | EC50 |
3.8 nM
Compound: Aldosterone
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Agonist activity at mineralocorticoid receptor ligand binding domain expressed in african green monkey COS7 cells co-transfected with Gal4-LBD by luciferase reporter gene assay
Agonist activity at mineralocorticoid receptor ligand binding domain expressed in african green monkey COS7 cells co-transfected with Gal4-LBD by luciferase reporter gene assay
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[PMID: 19863083] |
| HEK293 | EC50 |
0.18 nM
Compound: Ald
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Agonist activity at human MCR expressed in HEK293 cells by luciferase reporter gene assay
Agonist activity at human MCR expressed in HEK293 cells by luciferase reporter gene assay
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[PMID: 25305688] |
Aldosterone (1-1000 nM; 24 hours) inhibits interleukin-1β-stimulated nitrite production by vascular smooth muscle cells in a dose-dependent manner[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Aldosterone (0.72 mg/kg/day; 14 days) causes a small increase ( 14 mmHg) in blood pressure in male mice[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Forty male Wistar rats[4]
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Dosage:1 mg/Kg (+1% NaCl)
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Administration:i.h.; once daily for 3 weeks
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Result:Systolic blood pressure (SBP), diastolic blood pressure (DBP), left ventricular systolic pressure (LVSP) and left ventricular end-diastolic pressure (LVEDP) were significantly higher in aldosterone-salt-treated animals.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 52-39-1
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Appearance Solid
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Molecular Weight 360.44
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Formula C21H28O5
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Color White to off-white
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SMILES
C[C@@]12[C@@]3([H])[C@@H](O)C[C@]4(C=O)[C@@H](C(CO)=O)CC[C@@]4([H])[C@]3([H])CCC1=CC(CC2)=O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (10)
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Journal Impact Factor
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Most Recent
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Science
2026 Feb 5;391(6785):eadz4075. PMID: 41643022 -
Nat Chem Biol
2022 Nov;18(11):1196-1203. PMID: 35982227 -
Acta Pharmacol Sin
Discovery of a novel nonsteroidal selective glucocorticoid receptor modulator by virtual screening and bioassays. [Abstract]2022 Sep;43(9):2429-2438. PMID: 35110698
Aldosterone purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2022 Sep;43(9):2429-2438. [Abstract]
For MR selectivity, cells were treated with 10 nM Aldosterone or indicated compounds and cultured for 16 h.
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J Med Chem
Discovery of Novel Nonsteroidal SGRMs of Sulfonamide-2-Oxo-Tetrahydroquinoline Derivatives by Carbonyl Migration. [Abstract]2026 Jan 22;69(2):1507-1529. PMID: 41490153 -
J Med Chem
Discovery and Optimization of N-Acyl-6-sulfonamide-tetrahydroquinoline Derivatives as Novel Non-Steroidal Selective Glucocorticoid Receptor Modulators. [Abstract]2022 Dec 8;65(23):15710-15724. PMID: 36399795 -
Eur J Med Chem
Discovery of novel non-steroidal selective glucocorticoid receptor modulators by structure- and IGN-based virtual screening, structural optimization, and biological evaluation. [Abstract]2022 Jul 5:237:114382. PMID: 35483323 -
Cell Signal
Inhibitory effects of Eplerenone on angiogenesis via modulating SGK1/TGF-β pathway in contralateral kidney of CKD pregnancy rats. [Abstract]2024 Aug 13:111346. PMID: 39147296 -
Int J Endocrinol
G Protein-Coupled Estrogen Receptor 1 (GPER1) Mediates Aldosterone-Induced Endothelial Inflammation in a Mineralocorticoid Receptor-Independent Manner. [Abstract]2021 Jun 18:2021:5575927. PMID: 34239558
Aldosterone purchased from MedChemExpress. Usage Cited in: Int J Endocrinol. 2021 Jun 18:2021:5575927. [Abstract]
Aldosterone (1 h). The level of cell culture medium IL-1β was detected.
Aldosterone purchased from MedChemExpress. Usage Cited in: Int J Endocrinol. 2021 Jun 18:2021:5575927. [Abstract]
Aldosterone (1 h). ICAM-1 and VCAM-1 mRNA expressions were detected.
Aldosterone purchased from MedChemExpress. Usage Cited in: Int J Endocrinol. 2021 Jun 18:2021:5575927. [Abstract]
Aldosterone (1 h). Western blot of IL-1β and NLRP-3 was detected.
Aldosterone purchased from MedChemExpress. Usage Cited in: Int J Endocrinol. 2021 Jun 18:2021:5575927. [Abstract]
Aldosterone (1 h). The protein level of IL-1β from PBS to 10-9 M G15 was detected.
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Int Urol Nephrol
Aldosterone promotes calcification of vascular smooth muscle cells in mice through the AIF-1/Wnt/β-catenin signaling pathway. [Abstract]2025 Feb;57(2):613-623. PMID: 39312016 -
Solvent & Solubility
DMSO : 50 mg/mL (138.72 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Ethanol : 33.33 mg/mL (92.47 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.94 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.94 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.94 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.94 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (3.47 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (282 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Nanba K, et al. Aging and Adrenal Aldosterone Production. Hypertension. 2018 Feb;71(2):218-223. [Content Brief]
[2]. Cannavo A, et al. Aldosterone and Mineralocorticoid Receptor System in Cardiovascular Physiology and Pathophysiology. Oxid Med Cell Longev. 2018 Sep 19;2018:1204598. [Content Brief]
[3]. Ikeda U, et al. Aldosterone inhibits nitric oxide synthesis in rat vascular smooth muscle cells induced by interleukin-1 beta. Eur J Pharmacol. 1995 Jul 18;290(2):69-73. [Content Brief]
[4]. Martín-Fernández B, et al. Beneficial effects of proanthocyanidins in the cardiac alterations induced by aldosterone in ratheart through mineralocorticoid receptor blockade. PLoS One. 2014 Oct 29;9(10):e111104. [Content Brief]
[5]. Dinh QN, et al. Aldosterone-induced oxidative stress and inflammation in the brain are mediated by the endothelial cell mineralocorticoid receptor. Brain Res. 2016 Apr 15;1637:146-153. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Ethanol / DMSO | 1 mM | 2.7744 mL | 13.8719 mL | 27.7439 mL | 69.3597 mL |
| 5 mM | 0.5549 mL | 2.7744 mL | 5.5488 mL | 13.8719 mL | |
| 10 mM | 0.2774 mL | 1.3872 mL | 2.7744 mL | 6.9360 mL | |
| 15 mM | 0.1850 mL | 0.9248 mL | 1.8496 mL | 4.6240 mL | |
| 20 mM | 0.1387 mL | 0.6936 mL | 1.3872 mL | 3.4680 mL | |
| 25 mM | 0.1110 mL | 0.5549 mL | 1.1098 mL | 2.7744 mL | |
| 30 mM | 0.0925 mL | 0.4624 mL | 0.9248 mL | 2.3120 mL | |
| 40 mM | 0.0694 mL | 0.3468 mL | 0.6936 mL | 1.7340 mL | |
| 50 mM | 0.0555 mL | 0.2774 mL | 0.5549 mL | 1.3872 mL | |
| 60 mM | 0.0462 mL | 0.2312 mL | 0.4624 mL | 1.1560 mL | |
| 80 mM | 0.0347 mL | 0.1734 mL | 0.3468 mL | 0.8670 mL | |
| DMSO | 100 mM | 0.0277 mL | 0.1387 mL | 0.2774 mL | 0.6936 mL |
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