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  3. BAM7

BAM7 

Cat. No.: HY-15341 Purity: 99.18%
Handling Instructions

BAM7 is a direct and selective activator of proapoptotic BAX with an IC50 of 3.3 μM.

For research use only. We do not sell to patients.

BAM7 Chemical Structure

BAM7 Chemical Structure

CAS No. : 331244-89-4

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 149 In-stock
Estimated Time of Arrival: December 31
10 mg USD 135 In-stock
Estimated Time of Arrival: December 31
50 mg USD 650 In-stock
Estimated Time of Arrival: December 31
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Based on 2 publication(s) in Google Scholar

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Description

BAM7 is a direct and selective activator of proapoptotic BAX with an IC50 of 3.3 μM.

IC50 & Target[1]

Bax

3.3 μM (IC50)

In Vitro

BAM7 is selective for the BH3-binding site on BAX. BAM7 activates BAX and BAX-dependent cell death. Whereas treatment with BAX or BAM7 alone has no effect on the liposomes, the combination of BAM7 and BAX yields dose-responsive liposomal release of entrapped fluorophore. BAM7 dose- and time-responsively impairs the viability of Bak-/- MEFs that exclusively express BAX but has no effect on Bak-/- MEFs that contain BAK but lack BAX. In contrast, standard proapoptotic stimuli such as serum withdrawal, Staurosporine and Etoposide induces an equivalent apoptotic response in Bax-/- and Bak-/- MEFs. As further evidence of BAM7 specificity of action, (i) BAM7 does not affect the viability of Bax-/- Bak-/- MEFs; (ii) ANA-BAM16, which does not bind or activate BAX, has no effect on Bak-/- MEFs; and (iii) BAM7 selectively induces cell death of Bax-/- Bak-/- MEFs reconstituted with wild-type BAX but not BAXK21E , which bears the mutation that abrogates BAM7 binding[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

405.47

Formula

C₂₁H₁₉N₅O₂S

CAS No.

331244-89-4

SMILES

CCOC1=CC=CC=C1N/N=C2C(C)=NN(C3=NC(C4=CC=CC=C4)=CS3)C\2=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 5 mg/mL (12.33 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4663 mL 12.3314 mL 24.6627 mL
5 mM 0.4933 mL 2.4663 mL 4.9325 mL
10 mM 0.2466 mL 1.2331 mL 2.4663 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 0.5 mg/mL (1.23 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Cell Assay
[1]

MEF cells are maintained in DMEM high glucose supplemented with 10% (v/v) FBS, 100 U/mL Penicillin, 100 μg/mL Streptomycin, 2 mM L-glutamine, 50 mM HEPES, 0.1 mM MEM nonessential amino acids and 50 μM β-mercaptoethanol. MEFs (2.5×103 cells per well) are seeded in 96-well opaque plates for 18-24 h and then incubated with serial dilutions of BAM7 (3.75, 5, 7.5, 10 and 15 μM), ANA-BAM16 or vehicle (0.15% (v/v) DMSO) in DMEM at 37°C in a final volume of 100 μL. Cell viability is assayed at 24 h by addition of CellTiter-Glo reagent, and luminescence is measured using a SpectraMax M5 microplate reader. Viability assays are performed in at least triplicate, and the data are normalized to vehicle-treated control wells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
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Keywords:

BAM7BAM 7BAM-7Bcl-2 FamilyInhibitorinhibitorinhibit

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