1. Apoptosis
  2. Bcl-2 Family

Bax inhibitor peptide V5 (Synonyms: Val-Pro-Met-Leu-Lys; BIP-V5; BAX Inhibiting Peptide V5)

Cat. No.: HY-P0081 Purity: 99.79%
Handling Instructions

Bax inhibitor peptide V5 is a Bax-mediated apoptosis inhibitor, used for cancer treatment.

For research use only. We do not sell to patients.
Bax inhibitor peptide V5 Chemical Structure

Bax inhibitor peptide V5 Chemical Structure

CAS No. : 579492-81-2

Size Price Stock Quantity
5 mg USD 108 In-stock
10 mg USD 168 In-stock
25 mg USD 360 In-stock
50 mg USD 600 In-stock
100 mg   Get quote  
200 mg   Get quote  

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Customer Review

    Bax inhibitor peptide V5 purchased from MCE. Usage Cited in: Sci Rep. 2017 Jul 3;7(1):4546.

    Pretreatment with BIP-V5 significantly rescues cells from apoptosis induced by HDAC6 inhibition, whereas no effect is detected with a negative control peptide.

    Bax inhibitor peptide V5 purchased from MCE. Usage Cited in: Université Laval. 2017-08.

    Apoptosis is measured using Annexin V staining (green) in control (n=3 cell lines) and PAH-PASMCs (n=3 cell lines) pre-treated or not with Bax inhibitory peptides (BIP-V5, 200μM) or control peptides before being exposed to indicated HDAC6 inhibitors for 48 hours. Representative immunofluorescence images of apoptotic Annexin-V-positive cells (green) stained for DAPI (blue) and corresponding quantification are shown.
    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References

    Description

    Bax inhibitor peptide V5 is a Bax-mediated apoptosis inhibitor, used for cancer treatment.

    In Vitro

    Bax inhibitor peptide V5 (0-50 μM) reduces cell death in STF-cMyc cells but not in SW620 or NCI-H23 cells. BIPV5 does not result in any significant effect on cell cycle arrest at the G2/M phase[1]. V5 treatment upregulates expression of anti-apoptotic proteins Bcl-2 and XIAP by more than 3- and 11-fold and downregulates expression of apoptosis-inducing proteins Bax, Bad, and nuclear factor-κB-p65 by 10, 30, and nearly 50%, respectively[2].

    In Vivo

    V5 (100 μM) significantly improves islet function following isolation and improves islet graft function following transplantation in mice model[2].

    References
    Preparing Stock Solutions
    Concentration Volume Mass 1 mg 5 mg 10 mg
    1 mM 1.7042 mL 8.5209 mL 17.0419 mL
    5 mM 0.3408 mL 1.7042 mL 3.4084 mL
    10 mM 0.1704 mL 0.8521 mL 1.7042 mL
    Please refer to the solubility information to select the appropriate solvent.
    Cell Assay
    [1]

    Bax inhibitor peptide V5 is dissolved in DMSO.

    Cells (2.5×104 cells/mL) are grown in 96-well plates in a final volume of 100 μL/well. After 24 h, cells are incubated with small molecules or vehicle (DMSO) for 48-96 h prior to harvest. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a tetrazole (MTT) reagent (0.5 mg/mL), is added to each well during the final 2 h, and absorbance is measured. Cell growth is calculated as the ratio of absorbance obtained upon compound treatment to that obtained with vehicle (DMSO) treatment. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    Molecular Weight

    586.79

    Formula

    C₂₇H₅₀N₆O₆S

    CAS No.

    579492-81-2

    SMILES

    O=C([[email protected]](CCC1)N1C([[email protected]@H](N)C(C)C)=O)N[[email protected]@H](CCSC)C(N[[email protected]@H](CC(C)C)C(N[[email protected]](C(O)=O)CCCCN)=O)=O

    Storage
    Powder -80°C 2 years
      -20°C 1 year
    In solvent -80°C 6 months
      -20°C 1 month
    Shipping

    Room temperature in continental US; may vary elsewhere

    Solvent & Solubility

    DMSO: ≥ 30 mg/mL

    * "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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    Inquiry Information

    Product Name:
    Bax inhibitor peptide V5
    Cat. No.:
    HY-P0081
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