1. Apoptosis
  2. Bcl-2 Family Apoptosis
  3. Bax inhibitor peptide V5

Bax inhibitor peptide V5  (Synonyms: BIP-V5; BAX Inhibiting Peptide V5)

Cat. No.: HY-P0081 Purity: 98.28%
COA Handling Instructions

Bax inhibitor peptide V5 (BIP-V5) is a Bax-mediated apoptosis inhibitor, used for cancer treatment.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Bax inhibitor peptide V5 Chemical Structure

Bax inhibitor peptide V5 Chemical Structure

CAS No. : 579492-81-2

Size Price Stock Quantity
5 mg USD 119 In-stock
10 mg USD 185 In-stock
25 mg USD 396 In-stock
50 mg USD 660 In-stock
100 mg   Get quote  
200 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 19 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Bax inhibitor peptide V5 purchased from MedChemExpress. Usage Cited in: Toxicol Appl Pharmacol. 2018 Sep 15;355:269-285.  [Abstract]

    Bax inhibition ameliorates mitochondria dysfunction through modulating translocation and expression of Bax. H9c2 cells are treated with 160 nM TP for 24 h after pretreatment with BIP V5 (100 μM) or vehicle for 1 h. The cytoplasmic levels of Bax, Bcl-2, Cyt C, cleaved-caspase-9, cleaved-caspase-3 and β-actin are measured by immunoblot assays in H9c2 cells.

    Bax inhibitor peptide V5 purchased from MedChemExpress. Usage Cited in: Toxicol Appl Pharmacol. 2018 Sep 15;355:269-285.  [Abstract]

    Bax inhibition ameliorates mitochondria dysfunction through modulating translocation and expression of Bax. H9c2 cells are treated with 160 nM TP for 24 h after pretreatment with BIP V5 (100 μM) or vehicle for 1 h. The mitochondrial levels of Bax, Bak, Bcl-2, Cyt C, and COX VI are measured by western blot analysis in H9c2 cells.

    Bax inhibitor peptide V5 purchased from MedChemExpress. Usage Cited in: Sci Rep. 2017 Jul 3;7(1):4546.  [Abstract]

    Pretreatment with BIP-V5 significantly rescues cells from apoptosis induced by HDAC6 inhibition, whereas no effect is detected with a negative control peptide.

    Bax inhibitor peptide V5 purchased from MedChemExpress. Usage Cited in: Université Laval. 2017-08.

    Apoptosis is measured using Annexin V staining (green) in control (n=3 cell lines) and PAH-PASMCs (n=3 cell lines) pre-treated or not with Bax inhibitory peptides (BIP-V5, 200μM) or control peptides before being exposed to indicated HDAC6 inhibitors for 48 hours. Representative immunofluorescence images of apoptotic Annexin-V-positive cells (green) stained for DAPI (blue) and corresponding quantification are shown.

    View All Bcl-2 Family Isoform Specific Products:

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Bax inhibitor peptide V5 (BIP-V5) is a Bax-mediated apoptosis inhibitor, used for cancer treatment.

    IC50 & Target[1]

    Bax

     

    In Vitro

    Bax inhibitor peptide V5 (BIP-V5; 0-50 μM) reduces cell death in STF-cMyc cells but not in SW620 or NCI-H23 cells. BIPV5 does not result in any significant effect on cell cycle arrest at the G2/M phase[1]. V5 treatment upregulates expression of anti-apoptotic proteins Bcl-2 and XIAP by more than 3- and 11-fold and downregulates expression of apoptosis-inducing proteins Bax, Bad, and nuclear factor-κB-p65 by 10, 30, and nearly 50%, respectively[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    In Vivo

    Bax inhibitor peptide V5 (BIP-V5; 100 μM) significantly improves islet function following isolation and improves islet graft function following transplantation in mice model[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    586.79

    Formula

    C27H50N6O6S

    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    Sequence Shortening

    VPMLK

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    Sealed storage, away from moisture

    Powder -80°C 2 years
    -20°C 1 year

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 100 mg/mL (170.42 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 100 mg/mL (170.42 mM; Need ultrasonic)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.7042 mL 8.5209 mL 17.0419 mL
    5 mM 0.3408 mL 1.7042 mL 3.4084 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (4.26 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (4.26 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (170.42 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation
    References
    Cell Assay
    [1]

    Cells (2.5×104 cells/mL) are grown in 96-well plates in a final volume of 100 μL/well. After 24 h, cells are incubated with small molecules or vehicle (DMSO) for 48-96 h prior to harvest. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a tetrazole (MTT) reagent (0.5 mg/mL), is added to each well during the final 2 h, and absorbance is measured. Cell growth is calculated as the ratio of absorbance obtained upon compound treatment to that obtained with vehicle (DMSO) treatment.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 1.7042 mL 8.5209 mL 17.0419 mL 42.6047 mL
    5 mM 0.3408 mL 1.7042 mL 3.4084 mL 8.5209 mL
    10 mM 0.1704 mL 0.8521 mL 1.7042 mL 4.2605 mL
    15 mM 0.1136 mL 0.5681 mL 1.1361 mL 2.8403 mL
    20 mM 0.0852 mL 0.4260 mL 0.8521 mL 2.1302 mL
    25 mM 0.0682 mL 0.3408 mL 0.6817 mL 1.7042 mL
    30 mM 0.0568 mL 0.2840 mL 0.5681 mL 1.4202 mL
    40 mM 0.0426 mL 0.2130 mL 0.4260 mL 1.0651 mL
    50 mM 0.0341 mL 0.1704 mL 0.3408 mL 0.8521 mL
    60 mM 0.0284 mL 0.1420 mL 0.2840 mL 0.7101 mL
    80 mM 0.0213 mL 0.1065 mL 0.2130 mL 0.5326 mL
    100 mM 0.0170 mL 0.0852 mL 0.1704 mL 0.4260 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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