1. Apoptosis
  2. Bcl-2 Family
    Apoptosis
  3. Bax inhibitor peptide V5

Bax inhibitor peptide V5 (Synonyms: BIP-V5; BAX Inhibiting Peptide V5)

Cat. No.: HY-P0081 Purity: 99.62%
Handling Instructions

Bax inhibitor peptide V5 is a Bax-mediated apoptosis inhibitor, used for cancer treatment.

For research use only. We do not sell to patients.

Bax inhibitor peptide V5 Chemical Structure

Bax inhibitor peptide V5 Chemical Structure

CAS No. : 579492-81-2

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5 mg USD 108 In-stock
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10 mg USD 168 In-stock
Estimated Time of Arrival: December 31
25 mg USD 360 In-stock
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50 mg USD 600 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 8 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Bax inhibitor peptide V5 purchased from MCE. Usage Cited in: Toxicol Appl Pharmacol. 2018 Sep 15;355:269-285.

    Bax inhibition ameliorates mitochondria dysfunction through modulating translocation and expression of Bax. H9c2 cells are treated with 160 nM TP for 24 h after pretreatment with BIP V5 (100 μM) or vehicle for 1 h. The mitochondrial levels of Bax, Bak, Bcl-2, Cyt C, and COX VI are measured by western blot analysis in H9c2 cells.

    Bax inhibitor peptide V5 purchased from MCE. Usage Cited in: Toxicol Appl Pharmacol. 2018 Sep 15;355:269-285.

    Bax inhibition ameliorates mitochondria dysfunction through modulating translocation and expression of Bax. H9c2 cells are treated with 160 nM TP for 24 h after pretreatment with BIP V5 (100 μM) or vehicle for 1 h. The cytoplasmic levels of Bax, Bcl-2, Cyt C, cleaved-caspase-9, cleaved-caspase-3 and β-actin are measured by immunoblot assays in H9c2 cells.

    Bax inhibitor peptide V5 purchased from MCE. Usage Cited in: Sci Rep. 2017 Jul 3;7(1):4546.

    Pretreatment with BIP-V5 significantly rescues cells from apoptosis induced by HDAC6 inhibition, whereas no effect is detected with a negative control peptide.

    Bax inhibitor peptide V5 purchased from MCE. Usage Cited in: Université Laval. 2017-08.

    Apoptosis is measured using Annexin V staining (green) in control (n=3 cell lines) and PAH-PASMCs (n=3 cell lines) pre-treated or not with Bax inhibitory peptides (BIP-V5, 200μM) or control peptides before being exposed to indicated HDAC6 inhibitors for 48 hours. Representative immunofluorescence images of apoptotic Annexin-V-positive cells (green) stained for DAPI (blue) and corresponding quantification are shown.
    • Biological Activity

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    Description

    Bax inhibitor peptide V5 is a Bax-mediated apoptosis inhibitor, used for cancer treatment.

    IC50 & Target[1]

    Bax

     

    In Vitro

    Bax inhibitor peptide V5 (0-50 μM) reduces cell death in STF-cMyc cells but not in SW620 or NCI-H23 cells. BIPV5 does not result in any significant effect on cell cycle arrest at the G2/M phase[1]. V5 treatment upregulates expression of anti-apoptotic proteins Bcl-2 and XIAP by more than 3- and 11-fold and downregulates expression of apoptosis-inducing proteins Bax, Bad, and nuclear factor-κB-p65 by 10, 30, and nearly 50%, respectively[2].

    In Vivo

    V5 (100 μM) significantly improves islet function following isolation and improves islet graft function following transplantation in mice model[2].

    Molecular Weight

    586.79

    Formula

    C₂₇H₅₀N₆O₆S

    CAS No.

    579492-81-2

    Sequence

    Val-Pro-Met-Leu-Lys

    Sequence Shortening

    VPMLK

    SMILES

    O=C([[email protected]](CCC1)N1C([[email protected]@H](N)C(C)C)=O)N[[email protected]@H](CCSC)C(N[[email protected]@H](CC(C)C)C(N[[email protected]](C(O)=O)CCCCN)=O)=O

    Shipping

    Room temperature in continental US; may vary elsewhere

    Storage
    Powder -80°C 2 years
      -20°C 1 year
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 30 mg/mL (51.13 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.7042 mL 8.5209 mL 17.0419 mL
    5 mM 0.3408 mL 1.7042 mL 3.4084 mL
    10 mM 0.1704 mL 0.8521 mL 1.7042 mL
    *Please refer to the solubility information to select the appropriate solvent.
    References
    Cell Assay
    [1]

    Cells (2.5×104 cells/mL) are grown in 96-well plates in a final volume of 100 μL/well. After 24 h, cells are incubated with small molecules or vehicle (DMSO) for 48-96 h prior to harvest. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a tetrazole (MTT) reagent (0.5 mg/mL), is added to each well during the final 2 h, and absorbance is measured. Cell growth is calculated as the ratio of absorbance obtained upon compound treatment to that obtained with vehicle (DMSO) treatment.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
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    Mass   Concentration   Volume   Molecular Weight *
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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2

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    Product Name:
    Bax inhibitor peptide V5
    Cat. No.:
    HY-P0081
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