Cat. No.: HY-N6587

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Betulinic acid methyl ester

(Synonyms: Methyl betulinate)

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Betulinic acid methyl ester, a betulinic acid derivative, possesses antiprotozoal activity.

For research use only. We do not sell to patients.

Purity: 99.76%
CAS No.: 2259-06-5
Formula: C₃₁H₅₀O₃
Molecular Weight:470.73

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Betulinic acid methyl ester Chemical Structure

CAS No.: 2259-06-5

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Betulinic acid methyl ester, a betulinic acid derivative, possesses antiprotozoal activity^[1].

All Parasite Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
518A2	IC₅₀	16.93 μM Compound: 15	Cytotoxicity against human 518A2 cells assessed as cell viability after 96 hrs by SRB assay Cytotoxicity against human 518A2 cells assessed as cell viability after 96 hrs by SRB assay	[PMID: 23973824]
518A2	IC₅₀	28.75 μM Compound: 3	Cytotoxicity against human 518A2 cells after 96 hrs by SRB assay Cytotoxicity against human 518A2 cells after 96 hrs by SRB assay	[PMID: 20451375]
8505C	IC₅₀	17.17 μM Compound: 15	Cytotoxicity against human 8505C cells assessed as cell viability after 96 hrs by SRB assay Cytotoxicity against human 8505C cells assessed as cell viability after 96 hrs by SRB assay	[PMID: 23973824]
8505C	IC₅₀	23.67 μM Compound: 3	Cytotoxicity against human 8505C cells after 96 hrs by SRB assay Cytotoxicity against human 8505C cells after 96 hrs by SRB assay	[PMID: 20451375]
A253 cell line	IC₅₀	18.51 μM Compound: 3	Cytotoxicity against human A253 cells after 96 hrs by SRB assay Cytotoxicity against human A253 cells after 96 hrs by SRB assay	[PMID: 20451375]
A2780	IC₅₀	16.27 μM Compound: 15	Cytotoxicity against human A2780 cells assessed as cell viability after 96 hrs by SRB assay Cytotoxicity against human A2780 cells assessed as cell viability after 96 hrs by SRB assay	[PMID: 23973824]
A2780	IC₅₀	29.31 μM Compound: 3	Cytotoxicity against human A2780 cells after 96 hrs by SRB assay Cytotoxicity against human A2780 cells after 96 hrs by SRB assay	[PMID: 20451375]
A-431	IC₅₀	26.99 μM Compound: 3	Cytotoxicity against human A431 cells after 96 hrs by SRB assay Cytotoxicity against human A431 cells after 96 hrs by SRB assay	[PMID: 20451375]
A549	IC₅₀	17.17 μM Compound: 15	Cytotoxicity against human A549 cells assessed as cell viability after 96 hrs by SRB assay Cytotoxicity against human A549 cells assessed as cell viability after 96 hrs by SRB assay	[PMID: 23973824]
A549	IC₅₀	20.63 μM Compound: 3	Cytotoxicity against human A549 cells after 96 hrs by SRB assay Cytotoxicity against human A549 cells after 96 hrs by SRB assay	[PMID: 20451375]
B16	ED₅₀	2.5 μM Compound: 12	Induction of melanogenesis in mouse B16 2F2 cells assessed as intracellular melanin content after 3 days Induction of melanogenesis in mouse B16 2F2 cells assessed as intracellular melanin content after 3 days	[PMID: 12027734]
B16	IC₅₀	4.9 μM Compound: 12	Growth inhibition of mouse B16 2F2 cells after 3 days Growth inhibition of mouse B16 2F2 cells after 3 days	[PMID: 12027734]
CCRF-CEM	IC₅₀	150 μM Compound: 2	Cytotoxicity against human CEM cells after 72 hrs by MTT assay Cytotoxicity against human CEM cells after 72 hrs by MTT assay	[PMID: 15270560]
CHO	EC₅₀	>10 μM Compound: 34	Agonist activity at TGR5 expressed in CHO cells by CRE-driven luciferase reporter gene assay Agonist activity at TGR5 expressed in CHO cells by CRE-driven luciferase reporter gene assay	[PMID: 19911773]
COS-1	EC₅₀	0 μM Compound: 34	Agonist activity at human FXR expressed in COS1 cells by luciferase reporter gene assay Agonist activity at human FXR expressed in COS1 cells by luciferase reporter gene assay	[PMID: 19911773]
DLD-1	IC₅₀	45.86 μM Compound: 3	Cytotoxicity against human DLD1 cells after 96 hrs by SRB assay Cytotoxicity against human DLD1 cells after 96 hrs by SRB assay	[PMID: 20451375]
FaDu	IC₅₀	20.33 μM Compound: 3	Cytotoxicity against human FADU cells after 96 hrs by SRB assay Cytotoxicity against human FADU cells after 96 hrs by SRB assay	[PMID: 20451375]
HCT-116	IC₅₀	20.47 μM Compound: 3	Cytotoxicity against human HCT116 cells after 96 hrs by SRB assay Cytotoxicity against human HCT116 cells after 96 hrs by SRB assay	[PMID: 20451375]
HCT-8	IC₅₀	17.25 μM Compound: 3	Cytotoxicity against human HCT8 cells after 96 hrs by SRB assay Cytotoxicity against human HCT8 cells after 96 hrs by SRB assay	[PMID: 20451375]
HT-29	IC₅₀	30.72 μM Compound: 3	Cytotoxicity against human HT-29 cells after 96 hrs by SRB assay Cytotoxicity against human HT-29 cells after 96 hrs by SRB assay	[PMID: 20451375]
HT-29	IC₅₀	61 μM Compound: 2	Cytotoxicity against human HT-29 cells after 72 hrs by MTT assay Cytotoxicity against human HT-29 cells after 72 hrs by MTT assay	[PMID: 15270560]
K562	IC₅₀	65 μM Compound: 2	Cytotoxicity against paclitaxel-resistant human K562 cells after 72 hrs by MTT assay Cytotoxicity against paclitaxel-resistant human K562 cells after 72 hrs by MTT assay	[PMID: 15270560]
K562	IC₅₀	71 μM Compound: 2	Cytotoxicity against human K562 cells after 72 hrs by MTT assay Cytotoxicity against human K562 cells after 72 hrs by MTT assay	[PMID: 15270560]
KB	ED₅₀	11.8 μg/mL Compound: 16	Cytotoxicity against human epidermoid carcinoma of the mouth (KB) cell line. Cytotoxicity against human epidermoid carcinoma of the mouth (KB) cell line.	[PMID: 9873420]
MCF7	IC₅₀	16.93 μM Compound: 15	Cytotoxicity against human MCF7 cells assessed as cell viability after 96 hrs by SRB assay Cytotoxicity against human MCF7 cells assessed as cell viability after 96 hrs by SRB assay	[PMID: 23973824]
MCF7	IC₅₀	24.39 μM Compound: 3	Cytotoxicity against human MCF7 cells after 96 hrs by SRB assay Cytotoxicity against human MCF7 cells after 96 hrs by SRB assay	[PMID: 20451375]
PC-3	IC₅₀	109 μM Compound: 2	Cytotoxicity against human PC3 cells after 72 hrs by MTT assay Cytotoxicity against human PC3 cells after 72 hrs by MTT assay	[PMID: 15270560]
SK-MEL-2	ED₅₀	8.3 μg/mL Compound: 16	Cytotoxicity against cultured human melanoma (MEL-2) cell line. Cytotoxicity against cultured human melanoma (MEL-2) cell line.	[PMID: 9873420]
SW-1736	IC₅₀	32.1 μM Compound: 3	Cytotoxicity against human SW1736 cells after 96 hrs by SRB assay Cytotoxicity against human SW1736 cells after 96 hrs by SRB assay	[PMID: 20451375]
SW480	IC₅₀	17.92 μM Compound: 3	Cytotoxicity against human SW480 cells after 96 hrs by SRB assay Cytotoxicity against human SW480 cells after 96 hrs by SRB assay	[PMID: 20451375]

Chemical Information

CAS No. 2259-06-5
Appearance Solid
Molecular Weight 470.73
Formula C₃₁H₅₀O₃
Color White to off-white
SMILES

COC([C@]12[C@@]([C@@H](CC2)C(C)=C)([H])[C@]3([H])[C@@](CC1)([C@]4([C@]([C@@]5([C@@](C(C)([C@H](CC5)O)C)([H])CC4)C)([H])CC3)C)C)=O
Synonyms

Methyl betulinate
Structure Classification
- Terpenoids
- Triterpenes
Initial Source
- Plants
- other families
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 5 mg/mL (10.62 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.1244 mL

10.6218 mL

21.2436 mL

5 mM

0.4249 mL

2.1244 mL

4.2487 mL

10 mM

0.2124 mL

1.0622 mL

2.1244 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration Concentration

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Molecular Weight Molecular Weight

Dilution Calculator

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (271 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. D B Domínguez-Carmona, et al. Antiprotozoal activity of betulinic acid derivatives. Phytomedicine. 2010 Apr;17(5):379-82. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.1244 mL	10.6218 mL	21.2436 mL	53.1090 mL
	5 mM	0.4249 mL	2.1244 mL	4.2487 mL	10.6218 mL
	10 mM	0.2124 mL	1.0622 mL	2.1244 mL	5.3109 mL

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Betulinic acid methyl ester

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All Parasite Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Betulinic acid methyl ester

Bioactive Compound Library Max

Bioactive Compound Library

Anti-Infection Compound Library

Natural Product Library

Terpenoids Library

Traditional Chinese Medicine Active Compound Library

Antiparasitic Compound library

Plant-Sourced Natural Product Library

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Ancient Chinese Classical Formulas Traditional Chinese Medicine Active Compound Library

Antitussive and Antiasthmatic Traditional Chinese Medicine Active Compound Library

Diarrhea-Related Traditional Chinese Medicine Active Compound Library

Mongolian Medicine Compound Library

Mass Spectrometry Natural Product Library

All Parasite Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Betulinic acid methyl ester Related Classifications

HY-N6587 Related Classifications