Art. -Nr.: HY-15300

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Skepinone-L

(Synonyms: CBS3830)

2 Cited Publications

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Skepinone-L (CBS3830), a chemical probe, is a selective p38 mitogen-activated protein kinase inhibitor.

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Reinheit: 99.79%
CAS. Nr.: 1221485-83-1
Formel: C₂₄H₂₁F₂NO₄
Molecular Weight:425.42

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Skepinone-L Chemical Structure

CAS. Nr.: 1221485-83-1

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (2)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Skepinone-L (CBS3830), a chemical probe, is a selective p38 mitogen-activated protein kinase inhibitor.

IC₅₀ & Target

p38MAPK^[1].

Alle p38 MAPK Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
PBMC	IC₅₀	0.03 μM Compound: Chemical Probe: Compound 5	Anti-inflammatory activity against human derived high respond PBMC cells assessed as inhibition in stimulated IL-1beta cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay Anti-inflammatory activity against human derived high respond PBMC cells assessed as inhibition in stimulated IL-1beta cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay	[PMID: 22198732]
PBMC	IC₅₀	0.05 μM Compound: Chemical Probe: Compound 5	Anti-inflammatory activity against human derived high respond PBMC cells assessed as inhibition in stimulated IL-10 cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay Anti-inflammatory activity against human derived high respond PBMC cells assessed as inhibition in stimulated IL-10 cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay	[PMID: 22198732]
PBMC	IC₅₀	0.05 μM Compound: Chemical Probe: Compound 5	Anti-inflammatory activity against human derived high respond PBMC cells assessed as inhibition in stimulated TNF-alpha cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay Anti-inflammatory activity against human derived high respond PBMC cells assessed as inhibition in stimulated TNF-alpha cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay	[PMID: 22198732]
PBMC	IC₅₀	0.06 μM Compound: Chemical Probe: Compound 5	Anti-inflammatory activity against human derived high respond PBMC cells assessed as inhibition in stimulated INFgamma cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay Anti-inflammatory activity against human derived high respond PBMC cells assessed as inhibition in stimulated INFgamma cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay	[PMID: 22198732]
PBMC	IC₅₀	0.06 μM Compound: Chemical Probe: Compound 5	Anti-inflammatory activity against human derived medium respond PBMC cells assessed as inhibition in stimulated IL-10 cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay Anti-inflammatory activity against human derived medium respond PBMC cells assessed as inhibition in stimulated IL-10 cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay	[PMID: 22198732]
PBMC	IC₅₀	0.06 μM Compound: Chemical Probe: Compound 5	Anti-inflammatory activity against human derived medium respond PBMC cells assessed as inhibition in stimulated IL-8 cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay Anti-inflammatory activity against human derived medium respond PBMC cells assessed as inhibition in stimulated IL-8 cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay	[PMID: 22198732]
PBMC	IC₅₀	0.09 μM Compound: Chemical Probe: Compound 5	Anti-inflammatory activity against human derived high respond PBMC cells assessed as inhibition in stimulated IL-8 cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay Anti-inflammatory activity against human derived high respond PBMC cells assessed as inhibition in stimulated IL-8 cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay	[PMID: 22198732]
PBMC	IC₅₀	0.09 μM Compound: Chemical Probe: Compound 5	Anti-inflammatory activity against human derived medium respond PBMC cells assessed as inhibition in stimulated MCP-1 chemokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay Anti-inflammatory activity against human derived medium respond PBMC cells assessed as inhibition in stimulated MCP-1 chemokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay	[PMID: 22198732]
PBMC	IC₅₀	0.12 μM Compound: Chemical Probe: Compound 5	Anti-inflammatory activity against human derived high respond PBMC cells assessed as inhibition in stimulated IL-1alpha cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay Anti-inflammatory activity against human derived high respond PBMC cells assessed as inhibition in stimulated IL-1alpha cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay	[PMID: 22198732]
PBMC	IC₅₀	0.2 μM Compound: Chemical Probe: Compound 5	Anti-inflammatory activity against human derived high respond PBMC cells assessed as inhibition in stimulated IL-1ra cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay Anti-inflammatory activity against human derived high respond PBMC cells assessed as inhibition in stimulated IL-1ra cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay	[PMID: 22198732]
PBMC	IC₅₀	0.3 μM Compound: Chemical Probe: Compound 5	Anti-inflammatory activity against human derived medium respond PBMC cells assessed as inhibition in stimulated INFgamma cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay Anti-inflammatory activity against human derived medium respond PBMC cells assessed as inhibition in stimulated INFgamma cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay	[PMID: 22198732]
PBMC	IC₅₀	0.37 μM Compound: Chemical Probe: Compound 5	Anti-inflammatory activity against human derived medium respond PBMC cells assessed as inhibition in stimulated IL-1alpha cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay Anti-inflammatory activity against human derived medium respond PBMC cells assessed as inhibition in stimulated IL-1alpha cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay	[PMID: 22198732]
PBMC	IC₅₀	0.42 μM Compound: Chemical Probe: Compound 5	Anti-inflammatory activity against human derived high respond PBMC cells assessed as inhibition in stimulated IL-17A cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay Anti-inflammatory activity against human derived high respond PBMC cells assessed as inhibition in stimulated IL-17A cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay	[PMID: 22198732]
PBMC	IC₅₀	0.65 μM Compound: Chemical Probe: Compound 5	Anti-inflammatory activity against human derived high respond PBMC cells assessed as inhibition in stimulated IL-6 cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay Anti-inflammatory activity against human derived high respond PBMC cells assessed as inhibition in stimulated IL-6 cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay	[PMID: 22198732]
PBMC	IC₅₀	0.78 μM Compound: Chemical Probe: Compound 5	Anti-inflammatory activity against human derived medium respond PBMC cells assessed as inhibition in stimulated IL-6 cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay Anti-inflammatory activity against human derived medium respond PBMC cells assessed as inhibition in stimulated IL-6 cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay	[PMID: 22198732]
PBMC	IC₅₀	0.86 μM Compound: Chemical Probe: Compound 5	Anti-inflammatory activity against human derived medium respond PBMC cells assessed as inhibition in stimulated TNF-alpha cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay Anti-inflammatory activity against human derived medium respond PBMC cells assessed as inhibition in stimulated TNF-alpha cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay	[PMID: 22198732]
PBMC	IC₅₀	0.87 μM Compound: Chemical Probe: Compound 5	Anti-inflammatory activity against human derived medium respond PBMC cells assessed as inhibition in stimulated IL-1ra cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay Anti-inflammatory activity against human derived medium respond PBMC cells assessed as inhibition in stimulated IL-1ra cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay	[PMID: 22198732]
PBMC	IC₅₀	1.59 μM Compound: Chemical Probe: Compound 5	Anti-inflammatory activity against human derived medium respond PBMC cells assessed as inhibition in stimulated IL-1beta cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay Anti-inflammatory activity against human derived medium respond PBMC cells assessed as inhibition in stimulated IL-1beta cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay	[PMID: 22198732]
PBMC	IC₅₀	1.94 μM Compound: Chemical Probe: Compound 5	Anti-inflammatory activity against human derived high respond PBMC cells assessed as inhibition in stimulated IL-13 cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay Anti-inflammatory activity against human derived high respond PBMC cells assessed as inhibition in stimulated IL-13 cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay	[PMID: 22198732]
PBMC	IC₅₀	4.38 μM Compound: Chemical Probe: Compound 5	Anti-inflammatory activity against human derived medium respond PBMC cells assessed as inhibition in stimulated IL-13 cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay Anti-inflammatory activity against human derived medium respond PBMC cells assessed as inhibition in stimulated IL-13 cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay	[PMID: 22198732]
PBMC	IC₅₀	5.9 μM Compound: Chemical Probe: Compound 5	Anti-inflammatory activity against human derived medium respond PBMC cells assessed as inhibition in stimulated IP-10 chemokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay Anti-inflammatory activity against human derived medium respond PBMC cells assessed as inhibition in stimulated IP-10 chemokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay	[PMID: 22198732]
PBMC	IC₅₀	7.85 μM Compound: Chemical Probe: Compound 5	Anti-inflammatory activity against human derived medium respond PBMC cells assessed as inhibition in stimulated IL-17A cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay Anti-inflammatory activity against human derived medium respond PBMC cells assessed as inhibition in stimulated IL-17A cytokine release incubated for 2 hrs in the presence of LPS/SEB/aCD28 by Luminex based sandwich immunoassay	[PMID: 22198732]

In Vitro

Skepinone-L (CBS3830), the first ATP-competitive p38 MAPK inhibitor with excellent in vivo efficacy and selectivity. Therefore, Skepinone-L (CBS3830) is a valuable probe for chem. biol. research, and it may foster the development of a unique class of kinase inhibitors.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 1221485-83-1
Appearance Solid
Molecular Weight 425.42
Formel C₂₄H₂₁F₂NO₄
Color Light yellow to yellow
SMILES

O=C1C2=CC(OC[C@@H](CO)O)=CC=C2CCC3=C1C=CC(NC4=CC=C(C=C4F)F)=C3
Synonyms

CBS3830
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (2)

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Journal Impact Factor

Nat Commun

Human iPSC-based Modeling of Pulmonary Fibrosis Reveals p300/CBP Inhibition Suppresses Alveolar Transitional Cell State. [Abstract]

2026 Feb 12;17(1):1214. PMID: 41680175
Nat Commun

Attenuated growth factor signaling during cell death initiation sensitizes membranes towards peroxidation. [Abstract]

2025 Feb 25;16(1):1774. PMID: 40000627

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : ≥ 50 mg/mL (117.53 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.3506 mL

11.7531 mL

23.5062 mL

5 mM

0.4701 mL

2.3506 mL

4.7012 mL

10 mM

0.2351 mL

1.1753 mL

2.3506 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (5.88 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (5.88 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Koeberle, Solveigh C.; Romir, Johannes; Fischer, Stefan et al. Skepinone-L is a selective p38 mitogen-activated protein kinase inhibitor. Nature Chemical Biology (2012), 8(2), 141-143. [Content Brief]

[2]. Koeberle, Solveigh C.; Fischer, Stefan; Schollmeyer, Dieter et al. Design, Synthesis, and Biological Evaluation of Novel Disubstituted Dibenzosuberones as Highly Potent and Selective Inhibitors of p38 Mitogen Activated Protein Kinase. Journal of Medicinal Chemistry (2012), 55(12), 5868-5877. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.3506 mL	11.7531 mL	23.5062 mL	58.7655 mL
	5 mM	0.4701 mL	2.3506 mL	4.7012 mL	11.7531 mL
	10 mM	0.2351 mL	1.1753 mL	2.3506 mL	5.8765 mL
	15 mM	0.1567 mL	0.7835 mL	1.5671 mL	3.9177 mL
	20 mM	0.1175 mL	0.5877 mL	1.1753 mL	2.9383 mL
	25 mM	0.0940 mL	0.4701 mL	0.9402 mL	2.3506 mL
	30 mM	0.0784 mL	0.3918 mL	0.7835 mL	1.9588 mL
	40 mM	0.0588 mL	0.2938 mL	0.5877 mL	1.4691 mL
	50 mM	0.0470 mL	0.2351 mL	0.4701 mL	1.1753 mL
	60 mM	0.0392 mL	0.1959 mL	0.3918 mL	0.9794 mL
	80 mM	0.0294 mL	0.1469 mL	0.2938 mL	0.7346 mL
	100 mM	0.0235 mL	0.1175 mL	0.2351 mL	0.5877 mL

Keywords

Skepinone-L

2 Cited Publications

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Publications Citing Use of MedChemExpress (MCE) Skepinone-L

Alle p38 MAPK Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (2)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Skepinone-L Related Classifications

HY-15300 Related Classifications